• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.20 no.4
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• pp.236-240
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• 2010

This study demonstrates the use of a chemical containing potassium superoxide (KO2) to convert carbon dioxide (CO$_2$) in air to oxygen (O$_2$). A oxygen generating closed-circuit SCBA (self contained breathing apparatus) removes carbon dioxide by a chemical reaction with potassium dioxide that consumes the carbon dioxide and produces oxygen. Considering the disasters, there is a need to develop strategies to enable the introduction of self-contained self rescuers (SCSR). The potassium superoxide reacts with the wears breath to produce oxygen and absorb carbon dioxide. If the respiration rate of a person is 5 MET (metabolic equivalent), to say 30 L/min, at disaster such as fire, mass of potassium superoxide was evaluated as 33.3 g with yield and safety factor. Four researchers tested on a laboratory treadmill breathing through SCSRs in a closed circuit, it appears useable for 9 minutes.

• KSBB Journal
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• v.13 no.3
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• pp.223-230
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• 1998

Experiments for lignin peroxidase production have been conducted by aerobic fermentation of Phanerochaete chrysosporium under low shear rate and enriched oxygen environment. The result of flask cultures of white rot fungus indicated that high oxygen concentration and low shear force were essential for enhancement of lignin peroxidase production. Pentachlorophenol was readily degraded by lignin peroxidase produced in nutrient limited flask cultures. Polyurethane foam was fond to be an effective immobilization matrix of P. chrysosporium.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.3
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• pp.186-190
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• 2000

It has been reported that flashing light enhances microalgal biomass productivity and overall photosynthetic efficiency. The algal growth kinetics and oxygen production rates under flashing light with various flashing frequencies (5Hz-37 kHz) were compared with those under equivalent continuous light in photobioreactors. A positive flashing light effect was observed with flashing frequencies over 1kHz. The oxygen production rate under conditions of flashing light was slightly higher than that under continuius ligth. The cells under the hight, particularly at higher cell concentrations. When 37kHz flashing light was applied to an LED-based photobioreactor, the concentration was higher than that obtained under continuous light by about 20%. Flashing light may be a reasonable solution to overcome mutual shading, particularly in high-density algal cultures.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.172-176
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• 1996

Effect of culture conditions such as pH, temperature, agitation speed and oxygen transfer rate on xylitol production from xylose by Candide parapsilosis ATCC 21019 mutant was investigated in a jar fermentor. The initial concentration of xylosr was fixed at 50 g/l in this experiment. When pH was increased, cell growth and xylose consumption rate were increased, but maximum xylitol production was shown in the range of pH 4.5 and 5.5 with a yield of 0.68 g/g-xylose. The optimal temperature for xylitol production was determined to be $30^{\circ}C$. Considering the importance of dissolved oxygen tension, for xylitol production, the effect of oxygen transfer rate coefficient $(k_La)$ on fermentation parameters was carefully evaluated in the range of $20{\sim}85\;hr{-1}\;of\;k_La$ (corresponding to $100{\sim}300$rpm of agitation speed). The xylitol production was maximized at $30\;hr^{-1}\;of\;k_La$(150 rpm). A higher oxygen transfer rate supported better cell growth with lower xylitol yield. It was determined that maximum xylitol concentration, xylitol yield and productivity was 35.8 g/l, 71.6% and $0.58\;g/l{\sim}hr^{-1}$, respectively, at $30\;hr^{-1}\;of\;k_La$ In order to further increase xylitol productivity, ferementation using the concentrated biomass(20 g/l) was carried out at the conditions of pH 4.5, $30^{\circ}C$ and $30\;hr\;1$ of oxygen transfer rate. The final xylitol concentration of 40 g/l was obtained at 18 hours of culture time. From this result, it was calculated that xylitol yield was 80ft on the basis of xylose consumption and volumetric productivity was $2.22\;g/l{\sim}hr$ which was increased by $3{\sim}4$ fold compared with $0.5{\sim}0.7\;g/l-hr$ obtained in a normal fermentation condition.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.45-50
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• 2000

For the maximum production of pullulan from glucose as a carbon source, the effects of glucose concentration, pH and dissolved oxygen concentration on the cell growth and mass production of high-molecular weight pullulan by A. pullulans ATCC 42023 were evaluated. A. pullulans showed optimum pullulan productivity when glucose concentration was 0.3M (54g/L). And inhibitory effects on the cell growth and the pullulan production were observed at the glucose concentration higher than 0.3M (54g/L). The influence of pH control and dissolved oxygen on the pullulan production and growth of A. pullulans was studied. In shake-flasks, maximum pullulan production was obtained with $11.98g/{\ell}$ when initial pH was 6.5. In the batch fermentation, the maximum pullulan production of $13.31g/{\ell}$ was obtained with constant pH 4.5. And it was found that pullulan yield and synthesis rate increased with oxygen availability. For the production of commercially useful pullulan with high-molecular weight, a mixed carbon source, which was a mixture of glucose and glucosamine, was used for the pullulan fermentation with A. pullulans. On the basis of 5% mixed carbon source, culture with 3% glucosamine with 2% glucose was optimum condition for the production of high (M.W.> 1,000,000) and medium (M.W.> 200,000) molecular weight pullulan with considerable yields of cell mass and product. And the influence of pH control on the molecular weight of pullulan was studied in batch fermentation. It was found that the productivity of high-molecular weight pullulan with pH control at 6.5 was higher than that with no pH control.

• Journal of Life Science
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• v.20 no.10
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• pp.1433-1442
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• 2010

Parameters related to dissolved oxygen for the production of pullulan by Aureobasidium pullulans HP-2001 were optimized in 7 l and 100 l bioreactors. The optimal concentrations of glucose and yeast extract for the production of pullulan were 50.0 and 2.5 g/l, respectively, and its conversion rate from glucose was 37% at a flask scale. The optimal initial pH of the medium and temperature for cell growth were 7.5 and $30^{\circ}C$, whereas those for the production of pullulan were 6.0 and $25^{\circ}C$. The optimal agitation speed and aeration rate for cell growth were 600 rpm and 2.0 vvm in a 7 l bioreactor, whereas those for the production of pullulan were 500 rpm and 1.0 vvm. The production of pullulan with an optimized agitation speed of 500 rpm and aeration rate of 1.0 vvm was 18.13 g/l in a 7 l bioreactor. Maximal cell growth occurred without inner pressure, whereas the optimal inner pressure for the production of pullulan was 0.4 kgf/$cm^2$ in a 100 l bioreactor. The production of pullulan under optimized conditions in this study was 22.89 g/l in a 100 l bioreactor, which was 1.38 times higher than that without inner pressure.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.292-297
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• 1993

Aspergillus niger No. PFST-38 was grown on complex media in 30L agitated fermentors at various aeration rates and stirrer speeds. We could correlate the mixing time as a function of the Reynolds number and the apparent viscosity, as follows. ${\theta}_M=2.95\;\NRe^{-0.52},\;{\theta}_M=1.88\;{\eta_a}^{0.57}$ Also, the effects of the apparent viscosity (${\theta}_a$), the impeller rotational speed (N), the air flow rate ($V_s$), and the mixing time (${\theta}_M$) on the oxygen transfer coefficient, $K_L a$ were determined experimentally, and equated as follows. $K_La=12.04N^{0.88}Vs^{0.71}{n_a}^{-0.83},\;K_La=30.2N^{0.88}Vs^{0.71}{\theta_M}^{-1.45}$ $K_La$ increased as the agitation speed and the air flow rate increased. The rate of $K_La$ increase was dependent more on the rotational speed of impeller than on the air flow rate. The glucoamylase production increased with the increase of the agitation speed upto at 500 rpm and increased with the increase of air flow rate upto at 1.0 vvm. The values calculated from the above equation confirmed that the experimental maximum production of glucoamylase was achieved when the $K_La$ and the apparent viscosity of the broth were $260\;hr^{-1}$ and 1800 cps, respectively.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.8
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• pp.497-503
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• 2017

A theoretical study on oxygen flow is fundamental to comprehend the practical production of an oxygen respirator and its stability. In this study, an orifice-type pressure-reducing component was designed for the newly developed oxygen respirator, using the commercial CFD tool, COMSOL Multiphysics, which increases its operational time compared to the existing component. The orifice was optimized by changing the length by 3, 6, and 9 mm within the entire computational domain of the oxygen respirator. Based on an oxygen flow rate of 0.028 kg/s, the oxygen respirator equipped with the newly developed orifice satisfied the flow rate within 33% for a respirator inlet pressure of 300 bar, and within 32.7% for 50, 75, and 100 bar. In terms of component manufacturing, the orifice length was selected as 3 mm, which removes additional changes to the existing component.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.1
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• pp.48-56
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• 2018

Due to the growing concerns of energy resource depletion and environmental destruction, the mass production of microalgae has been studied. The scale-up of a photobioreactor (PBR) is required for the mass production of biomass. In this paper, the geometric parameters and oxygen transfer rate (OTR) are considered, to scale up a flat panel photobioreactor (FP PBR). The PBR is designed using the goal-driven optimization (GDO) method to accomplish the scale-up. The local sensitivity of each output parameter with respect to the input parameter is analyzed through the design of experiment (DOE), and the design candidates are evaluated with the screening sampling method. The volumetric mass transfer coefficient is measured by the dynamic method.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.284-284
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• 2010

Currently, hydrogen has been produced by Steam Reforming or partial oxidation reforming processes mainly from oil, coal, and natural gas and results in the production of $CO_2$. However, these are influenced greatly on the green house effect of the earth. so it is important to find the new way to produce hydrogen utilizing water without producing any environmentally harmful by-products. In our research, we use microwave water plasma and photocatalyst to improve dissociation rate of water. At low pressure plasma, electron have high energy but density is low, so temperature of reactor is low. This may cause of recombination in the generated hydrogen and oxygen from splitting water. If it want to high dissociation rate of water, it is necessary to control of recombination of the hydrogen and oxygen using photocatalyst. We utilize the photocatalytic material($TiO_2$, ZnO) coated plasma reactor to use UV in the plasma. The quantity of hydrogen generated was measured by a Residual Gas Analyzer.