• KSBB Journal
• /
• v.17 no.2
• /
• pp.142-147
• /
• 2002

The decomposition of hydrogen peroxide was used for supplementing the oxygen during batch xanthan fermentations in a bubble column bioreactor in order to escape the oxygen transfer limitation that occurred at the high viscosity of culture broths. The xanthan production, however, was inhibited reversibly by dosing hydrogen peroxide. On the other hand, fluidized particles of glass beads with 8 mm diameter led to high gas-liquid oxygen transfer rates in three-phase fluidized beds, which resulted in higher space-time yields of the xanthan production compared to in the bubble column bioreactors.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.475-478
• /
• 2000

For an integrated nitrification-denitrification process, nitrite formation in the aerobic stage leads to big savings. Dissolved oxygen concentration, temperature, pH and free ammonia concentration have been meet for nitrite accumulation. Also their effects over the ammonia oxidizers and nitrite oxidizers have been studied. Dissolved oxygen limitation and free ammonia inhibition led to slow nitrification and nitrite build up. In this study batch kinetics of ammonium and nitrite oxidations were performed with free ammonia accumulated nitrifiers. From the results it is likely the nitrite oxidizers are inhibited by oxygen limitation rather than free ammonia.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.5
• /
• pp.593-603
• /
• 1999

Poly(3-hydroxybutyrate) (PHB) production by fermentation was examined under both restricted- and ample-oxygen supply conditions in a single fed-batch fermentation. Recombinant Escherichia coli transformed with the PHB production plasmid pSYLl07 was grown to reach high cell density (227 g/l dry cell weight) with a high PHB content (78％ of dry cell weight), using a glucose-based minimal medium. A simple flux model containing 12 fluxes was developed and applied to the fermentation data. A superior closure (95％) of the carbon mass balance was achieved. When the data were put into use, the results demonstrated a surprisingly large excretion of formate and lactate. Even though periods of severe oxygen limitation coincided with rapid acetate and lactate excretion, PHB productivity and carbon utilization efficiency were not significantly impaired. These results are very positive in reducing oxygen demand in an industrial PHA fermentation without sacrificing its PHA productivity, thereby reducing overall production costs.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.3 no.2
• /
• pp.109-111
• /
• 1998

The effect of dissolved oxygen concentration on the metabolism of glucose in Pseudomonas putida BM014 was investigated. Glucose was completely converted to 2-ketogluconate via extracellular oxidative pathway and then taken up for cell growth under the condition of sufficient dissolved oxygen concentration. On the other hand, oxygen limitation below dissolved oxygen tension (DOT) value of 20% of air saturation caused the shift of glucose metabolism from the extracellular oxidative pathway to the intracellular phosphorylative pathway. Specific activities of hexokinase and gluconate kinase in intracellular phosphorylation pathway decreased as the DOT increased, while 2-ketogluconokinase activity in extracellular oxidative pathway increased under the same condition. This result can be usefully applied to microbial transformation of glucose to 2-ketogluconate, the synthetic precursor for iso-vitamine C, with almost 100% yield via extracellular oxidation by simple DOT control.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.269-270
• /
• 2002

Oxygen is a key substrate in animal cell metabolism and its consumption is thus a parameter of great interest for monitoring and control in animal cell culture bioreactor. The use of a gas-permeable membrane offered the possibility to provide the required quantity of oxygen into the culture. while avoiding problems of foaming or shear damage generally linked to sparging. For determining the optimum DO control strategy of this gas-permeable membrane aeration bioreactor, the oxygen transfer rate coefficient was measured with varying $N_2$ ratio in inlet air. The results showed that an increasing mass flow rate of nitrogen reduced the $K_La$ value. and 5% nitrogen in air did not result in any oxygen limitation.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.11
• /
• pp.1690-1698
• /
• 2006

In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

• Journal of Korean Society on Water Environment
• /
• v.26 no.1
• /
• pp.156-159
• /
• 2010

Peroxide is used frequently to provide electron acceptors to aerobes for the purpose of in situ bioremediation of contaminated soil/sediment. In this study, oxygen release rate of peroxides and factors affecting on dissolution and diffusion of oxygen into pore water were evaluated. Peroxides studied in this study were magnesium peroxide ($MgO_2$), calcium peroxide ($CaO_2$), and sodium percarbonate ($Na_2CO_3{\cdot}1.5H_2O_2$). $Na_2CO_3{\cdot}1.5H_2O_2$ showed the highest oxygen release rate per unit mass and the shortest release duration time among three peroxides. A simple first-order decay model for predicting the release rate of oxygen from peroxide into pore water was presented and used to fit the experimental data. The first order oxygen release rate constants k for $MgO_2$, $CaO_2$ and $Na_2CO_3{\cdot}1.5H_2O_2$ were 0.45 /hr, 3.22 /hr and 134 /hr, respectively. If $MgO_2$ was mixed with clay, oxygen release rate was lowered significantly mainly due to limitation of contact area and diffusion, implying that oxygen can be provided to the indigenous aerobes for the extended period of time.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.12 no.2
• /
• pp.275-284
• /
• 1992

A generated problem in treated highly concentrated organic wastewater by activated sludge process is the limitation of biomass concentration and oxygen transfer capability in aeration tank. To overcome the limitation, the deep shaft activated sludge process which has high oxygen transfer capability was applied to the wastewater treatment process. This paper investigated the characteristics of liquid circulation, oxygen transfer and biological treatment of paper mill wastewater by the deep shaft activated sludge process. From the obtained results, it was found that the oxygen transfer capability in the deep shaft system was much greater than those in the conventional aeration systems and almost tantamount to the pure oxygen system. The deep shaft system could treat highly concentrated organic wastewater by higher biomass concentration and organic loading rate.

• Journal of Microbiology and Biotechnology
• /
• v.5 no.2
• /
• pp.100-104
• /
• 1995

Production of $poly-{\beta}-hydroxybutyrate$ (PHB) in a strain of Azotobacter chroococcum, a nitrogen-fixing bacteria, was investigated at various levels of nitrogen and oxygen. Feeding nitrogen source increased both cell growth and PHB accumulation. Oxygen supply appeared to be one of the most important operating parameters for PHB production. Both cell growth and PHB accumulation increased with the sufficient supply of air in the fed-batch fermentation of the strain. However, it was also noted that keeping the oxygen level under limited condition was critical to achieve high PHB productivity. A high titer of PHB (52 g/l) with a high cellular content (60%) was obtained after 48 hr of fed-batch operation by controlling the oxygen supply. Dual limitation of nitrogen and oxygen did not further increase the PHB accumulation probably due to the greater demand for reducing power and ATP for nitrogen fixation.

• Environmental Engineering Research
• /
• v.12 no.4
• /
• pp.148-154
• /
• 2007

The purpose of this research was to investigate the effects of low concentration of oxygen on reduction of perchlorate, especially low perchlorate influent concentrations in a biofilm reactor, as well as the effect of flow pattern in a biofilm reactor. Dissolved oxygen averaging 1 mg/L did not inhibit reduction of influent perchlorate from 23 to $426\;{\mu}g/L$ in the biofilm reactors when sufficient acetate was added, probably due to limitation of oxygen diffusion into the biofilm. Influent perchlorate ranging from 23 to $426\;{\mu}g/L$ was reduced to below detection level ($4\;{\mu}g/L$) in the presence of 1 mg/L dissolved oxygen (DO). Chloride was produced in a ratio of $0.37gCl^-/g{ClO_4}^-$ and $0.35gCl^-/g{ClO_4}^-$ in plug flow and recirculation biofilm reactor which is similar to stoichiometric amount ($0.36gCl^-/g{ClO_4}^-$) indicating complete perchlorate reduction at $426\;{\mu}g/L$ of ${ClO_4}^-$ feeding. At $23\;{\mu}g/L$L influent perchlorate, total biomass solids were 3.18 g and 2.81 g in the plug flow and recirculation biofilm reactors. The most probable number(MPN) analysis for perchlorate-reducing bacteria showed $10^4$ to $10^5\;cells/cm^2$ in both biofilm reactors throughout the experiments. The effluent perchlorate concentrations were not significantly different in the two different flow regimes, plug flow and recirculation biofilm reactors.