• 생명과학회지
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• 제13권5호
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• pp.692-698
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• 2003

뇌 세포막 유동성의 촉진 및 LPO생성의 억제효과는 중풍 등 뇌혈관질환을 억제할 수 있을 뿐만 아니라 혈관성치매의 예방에도 크게 기여할 것으로 기대된다[26,18]. 따라서 솔잎 EtOAc획분을 하루 25, 50, 100 mg/kg BW로써 SD계 랫트에 45일동안 투여하여 뇌조직중의 세포막 유동성 (membrane fluidity : MF), 기초 및 유도활성산소(BOR 및 IOR), 산화적 스트레스로서 과산화지질(lipid peroxide : LPO) 및 산화단백질(oxidized protein : OP), 그리고 리포푸신(lipofuscin : LF)의 축적에 미치는 영향을 평가하여 보았다. 솔잎 EtOAc획분의 투여에 의하여 뇌조직의 mitochondria 및 microsome획분에서 용량의존적으로 MF가 증가효과가 나타났지만, EtOAc-100 투여그룹의 mitochondria 획분에서만 약 10%의 유의적인 MF의 증가효과가 인정되었다. 뇌조직의 mitochondria에서 기초 및 유도활성산소로서 BOR 및 IOR의 생성은 EtOAc-50 및 EtOAc-100투여그룹에서 각각 9.2∼10.2% 및 17.1∼24.0%의 유의적인 억제효과가 인정되었고, microsomes에서 BOR 및 IOR의 생성은 EtOAc-50및 EtOAc-100투여그룹에서 각각 11.6∼16.6% 및 12.1∼16.1%의 유의적인 억제효과가 인정되었다. 산화적 스트레스에 미치는 영향은 mitochondria 및 microsomes획분에서 EtOAc-50 및 EtOAc-100투여그룹에서 각각 8.5∼12.0% 및 11.6∼18.5%의 유의적인 LPO의 생성 억제효과가 인정되었지만, 뇌조직의 두 획분에서 OP의 생성은 EtOAc획분의 용량의존적으로 억제되었지만, 유의성은 인정할 수 없었다. 또한 뇌조직에서 LF의 축적도 EtOAc획분의 용량 의존적으로 억제효과가 나타났지만, 유의성은 인정할 수 없었다. 이상의 결과에서 평가하여 볼 때 솔잎 EtOAc획분의 투여는 뇌조직의 유동성을 촉진하고 LPO의 생성을 효과적으로 억제할 수 있지만, 현재의 투여농도에서는 OP 및 LF의 생성을 방지할 수 없을 것으로 생각된다.

• 생명과학회지
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• 제13권5호
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• pp.684-691
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• 2003

솔잎 EtOAc획분을 하루 25, 50, 100 mg/kg체중으로서 SD계 랫트에 45일동안 투여하여 간장획분의 세포막 유동성(membrane fluidity MF), 기초 및 유도활성산소(BOR 및 IOR), 산화적 스트레스로서 과산화지질(lipid peroxide : LPO) 및 산화단백질(oxidized protein : OP), 그리고 리포푸신(lipofuscin : LF)의 축적에 미치는 영향을 평가하여 보았다. 솔잎 EtOAc획분의 투여에 의하여 간장의 mitochondria 및 microsome획분에서 어느 정도 MF가 증가하였지만, 유의성은 EtOAc-100 투여그룹의 mitochondria획분에서만 인정되었다. 기초 및 유도활성산소로서 BOR 및 IOR의 생성 억제효과는 간장의 두 획분의 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 활성산소의 생성 억제 효과가 인정되었다. 산화적 스트레스에 미치는 영향에서는 mitochondria획 분에서 는 EtOAc-100투여그룹에서, 그리고 microsome획분에서는 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 LPO의 생성 억제효과가 인정되었고, mitochondria 획분에서는 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 OP의 생성 억제효과가 인정되었지만, microsome 획분에서는 아무런 유의성도 인정할 수 없었다. 그렇지만, 간장의 클로로포름층에서 측정한 LF의 축적은 EtOAc-25, EtOAc-50 및 EtOAc-100 투여그룹에서 다같이 유의적인 LF의 축적 억제효과가 인정되었다. 이상의 결과에서 평가하여 볼 때 솔잎 EtOAc획분의 투여는 간장의 유동성을 촉진하고 산화적 스트레스를 효과적으로 억제할 수 있을 것으로 기대된다.

• 생명과학회지
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• 제11권2호
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• pp.103-110
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• 2001

This study was designed to investigate the effects of silkworm(Bombyx moril L.) powder on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10 g) were fed basic diet(control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400mg/kg BW/day for 6 weeks. There were no significant differences in cholesterol levels of brain memberanes by administration of silkworm powder (SWP). Membrane fluidities were significantly increased(21.5% and 30.8%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but significant difference between brain microsomes could not obtained. Basal oxygen radicals (BORs) in brain mitochondria and mircrosomes were significantly inhibited(8.5% and 16.5%, 16.8%and 24.8%, respectively) by SWP-200 and SEP-400 groups compared with control group. Induced oxygen radicals(IORs) in brain mitochondria were significantly inhibited(16.6% and 21.4%, respectively)by sWP-200 and SWP-400 groups compared with control group, but IOR in brain microsome were significantly inhibited about 16.0% by SWP-400 groups only compared with control group. Lipid peroxide(LPO) levels were significantly decreaed(14.8%and 22.4%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but LPO level was significantly decreased about 16.0% in brain microsome of SWP-400 group only. Oxidized protein(OP) levels were remarkably decreased(about 14.8% and 16.5%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups, but OP level was significantly decreased about 13.0% in brain microsome of SWP-400 group only compared with control group, Theses results suggest that administration of in brain microsome of SWP-400 group only compared with control group. These results suggest that administration of SWP may play effective role in attenuating an oxidative stress and increasing a membrane fluidity in brain membranes.

• 생명과학회지
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• 제10권5호
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• pp.496-503
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• 2000

This study was designed to investigate the effects of silkworm (Bombyx mori L.) powder on oxidative stress and membrane fluidity in liver membranes of rats. Sprague-Dawley (SD) male rats (160±10 g) were fed basic diet (control group), and experimental diets (SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. A significant differences between liver mitochondria and microsomes of SWP-200 and SWP-400 groups could not be obtained. Membrane fluidities were dose-dependently increased (14.8% and 28.5%, 20.0% and 29.9%) in liver mitochondria and microsomes of SWP-200 and SWP-400 groups compared with control group. Basal oxygen radicals (BOR) in liver mitochondria and mocrosomes were significantly inhibited (15.2% and 21.7%, 12.6% and 18.6%, respectively) by SWP-200 and SWP-400 groups compared with control group. Induced oxygen radicals (IOR) in liver microsomes were significantly inhibited (15.5% and 16.1%, respectively) by SWP-200 and SWP-400 groups compared with control group, but IOR in liver mitochondria was significantly inhibited about 12.0% by SWP-400 group only compared with control group. Lipid peroxide (LPO) levels were significantly decreased (14.4% and 9.1%, respectively) in liver mitochondria and microsomes of SWP-400 group only compared with control group. Oxidized protein (OP) levels were remarkably decreased about 12.7% and 16.3% in liver microsomes only of SWP-200 and SWP-400 groups, but significant difference between liver motochondria could not obtained. These results suggest that administration of SWP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in liver membranes.

• 생명과학회지
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• 제10권5호
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• pp.504-510
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• 2000

This study was designed to investigate the effects of mulberry (Morus alba L.) leaf extract (MLE) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (MLE-100 and MLE-300 groups) added 100 and 300 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a significant decreases (15.2% and 18.1%, 5.6% and 8.0%, respectively) in liver mitochondria and microsomes could be not obtained. These are no significant differences in superoxide radical ($O_2$) levels of liver cytosol in MLE-100 and MLE-300 groups compared with control group. Lipid peroxide (LPO) levels were slightly decreased about 13.6% and 6.1% in liver mitochondria and microsomes of MLE-300 group compared with control group. Oxidized protein (OP) levels were remarkably decreased about 16.9% and 27.2% in liver microsomes only of MLE-100 and MLE-300 group compared with control group. Mn-SOD activities in liver mitochondria were remarkably increased (18.2% and 28.7%, respectively) in MLE-100 and MLE-300 groups, and Cu,Zn-SOD activities in liver cytosol were also significantly increased (11.3% and 20.2%, respectively) in MLE-100 and MLE-300 groups compared with control group. Mn-SOD activities in liver mitochondria were remarkably increased (18.2% and 28.7%, respectively) in MLE-100 and MLE-300 groups, and Cu,Zn-SOD activities in liver cytosol were also significantly increased (11.3% and 20.2%, respectively) in MLE-100 and MLE-300 groups compared with control group, but significant difference between GSHPx activities in liver cytosol could be not obtained. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various age-related changes.

• 생명과학회지
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• 제10권5호
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• pp.511-518
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• 2000

This study was designed to investigate the effects of silk fibroin powder (SFP : Mw 500) on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Cholesterol level was significantly decreased about 8.0% in brain microsomes of SFP-5.0 group only compared with control group. Membrane fluidities were significantly increased (12.9% and 15.2%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups, but significant difference between in brain mitochondria of these two groups could be not obtained. Basal oxygen radicals (BOR) in brain mitochondria and microsomes were significantly ingibited (10.4%, and 24.0%, 7.9% and 14.9%, respectively) by SFP-2.5 and SFP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in brain mitochondria and microsomes were significantly inhibited (11.8% and 14.1%, respectively) by SFP-5.0 groups compared with control group compared with control group. Lipid peroxide (LPO) levels were dose-dependently decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (15.7% and 17.1%, 16.7% and 15.7%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in brain membranes.

• 한국잠사곤충학회지
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• 제42권1호
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• pp.58-64
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• 2000

This study was designed to investigate the effects of silk fibroin powder (Mw 500) on oxidative stress and membrane fluidity in liver membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10g) were fed basic diet (control group), and experimental diets (SEP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Cholesterol levels resulted in a significant decrease (12.1% and 9.0%, respectively) in the liver mitochondria and microsomes of SEP-5.0 group compared with control group. Membrane fluidity as significantly increased (16.1% and 16.5%, 5.8% and 17.4%) in the liver mitochondria and microsomes were significantly inhibited (16.1% and 18.3%, 8.1% and 15.1%, respectively) at the SFP-2.5 and SEP-5.0 groups compared with control group. Induced oxygen radicals (BOR) in liver mitochondria and microsomes were significantly inhibited (16.1% and 18.3%, 8.1% and 15.1%, respectively) at the SFP-2.5 and SEP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in liver microsomes were significantly inhibited (17.0% and 26.6%, respectively) at the SFP-2.5 and SFP-5.0 groups compared with control group, but IOR in liver mitochondria was significantly inhibited about 12.3% at the SWP-400 group only compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in the liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were dose-dependently decreased (5.4% and 11.6%, 19.0% and 24.4%, respaectively) in the iver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in attenuating an oxidative stress and increasing a membrane fluidity in liver membranes.

• 생명과학회지
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• 제10권4호
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• pp.354-361
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• 2000

The effect of mulberry (Morus alba L.) leaf extract(MLE) on oxidative stress and membrane fluidity in brain membranes of SD rats fed with 100 and 300 mg/kg BW/day were carried out for 6 weeks. Cholesterol accumulations resulted in a consistent decreases (4.6% and 5.6%, respectively) in brain mitochondria and microsomes of MLE-300 group compared with control group. Membrane fluidities were dose-dependently increased (2.2% and 5.1%, 5.0% and 15.2%) in brain mitochondria and microsomes of MLE-100 and MLE-300 groups compared with control group. Basal oxygen radicals(BORs) in brain mitochondria and microsomes were significantly inhibited (15.7% and 25.1%, 9.0% and 12.4%, respectively) by MLE-100 and MLE-300 groups compared with control group. Induced oxygen radicals(IORs) in brain mitochondria and microsomes were significantly inhibited (8.9% and 13.1%, 16.5% and 23.2%, respectively) by MLE-100 and MLE-300 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.5% and 18.1%, 7.6% and 12.3%) in brain mitochondria and microsomes of MLE-100 and MLE-300 groups compared with control group. Oxidized protein (OP) levels were dose-dependently decreased (4.3% and 14.2%, 10.0% and 10.9%, respectively) in brain microsomes of MLE-100 and MLE-300 groups compared with control group. These results suggest that MLE may play an effective role in an attenuating an oxidative stress and increasing a membrane fluidity in brain membranes.

• 생명과학회지
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• 제10권6호
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• pp.570-576
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• 2000

This study was designed to investigate the effects of mulberry (Morus alba L.) leaf extract (MLE) on oxygen radicals and their scavenger enzymes in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (MLE-100 and MLE-300 groups) added 100 and 300 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) lecels resulted in significant decreases (13.4% and 21.1%, 12.0% and 13.4%, respectively) in brain mitochondria and microsome of MLE-100 and MLE-300 groups compared with control group. Superoxide radical ($O_2$) levels were significantly decreased about 12% in brain cytosol of MLE-300 group compared with control group. Lipid peroxide (LPO) levels were effectively inhibited (18.1% and 12.3%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (14.2%, and 10.9%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Mn-SOD activities in brain mitochondria were significantly increased (13.5% and 18.6%, respectively) in MLE-100 and MLE-300 groups, and Cu/Zn-SOD activities in brain cytosol were also effectively increased (about 17.7%) in MLE-300 groups compared with control group. GSHPx activities in brain cytosol were remarkably increased (17.2% and 23.9%, respectively) in MLE-100 and MLE-300 groups compared with control group. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various age-related changes in brain.

• 생명과학회지
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• 제10권6호
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• pp.577-583
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• 2000

This study was designed to investigate the effect of silk fibroin (Mw 500)power (SFP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a considerable decreases (5.8% and 8.4%, 3.7% and 11.1%, respectively) in liver motochondria and micorsomes of SFP-2.5 and SFP-5.0 groups compared with control group, and $O_2$radical level was remarkably decreased about 15% and 20% in liver cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were remarkably decreased about 11.6% in liver mitochondria of SFP-5.0 group compared with control group. Mn-SOD activities were remarkably in creased (17.6% and 28.8%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups, and Cu/Zn-SOD activities were also effectively in creased (about 14.4%) in liver cytosol of SFP-5.0 groups, but significant difference between GSHPx activity in liver cytosol of these two groups could be not obtained. These results suggest that anti-aging effect of silk fibroin may play an effective anti-aging role in a aattenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.