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http://dx.doi.org/10.5352/JLS.2003.13.5.684

Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Liver Membranes of Rats  

최진호 (부경대학교 생화학교실)
김대익 (부경대학교 생화학교실)
백승진 (부경대학교 생화학교실)
박시향 (부경대학교 생화학교실)
김남주 (부경대학교 생화학교실)
최민경 (최민경 요리아카데미)
조원기 (㈜조아제약)
김창목 (한국산업정보기술연구원 생화학실)
Publication Information
Journal of Life Science / v.13, no.5, 2003 , pp. 684-691 More about this Journal
Abstract
This study was designed to investigate the effects of ethyl acetate (EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle extract on membrane fluidity (MF), basal and induced oxygen radical (BOR and IOR), lipid peroxide(LPO) and oxidized protein (OP) as an oxidative stress, and lipofuscin(LF) in liver membranes of male Sprague-Dawley rats. Rats were fed basic diets (control) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45days. MFs were significantly increased (about 10%) in mitochondria of EtOAc-100 group compared with control group. BOR and IOR formations in mitochondria were significantly inhibited (about 12∼18% and 9 ∼l2%, respectively) in EtOAc-50 and EtOAc-100 groups, while BOR and IOR formations in microsomes were significantly inhibited (about 9∼l3% and 18∼19%, respectively) compared with control group. LPO levels were significantly inhibited (about 10% and 12∼13%, respectively) in mitochondria of EtOAc-100 and microsomes of EtOAc-50 and EtOAc-100 groups, whereas OP levels were significantly inhibited (about 13∼14%) in mitochondria of EtOAc-50 and EtOAc.-100 groups compared with control group. LF formations were significantly inhibited (about 10∼14%) in these three EtOAc groups. These results suggest that ethyl acetate fraction of pine needle may play an effective role in attenuating an oxidative stress and increasing a membrane fluidity.
Keywords
Pine (Pinus densiflora Sieb et Zucc); Lipid peroxide (LPO); Oxidized protein; Carbonyl group; Membrane fluidity (MF); Lipofuscin (LF);
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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