• Title/Summary/Keyword: ovicidal effect

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Screening for Herbs Having Inhibitory Effect on Egg Hatching of Strongyloides venezuelensis

  • Kim, Byeong-Soo;Baek, Byeong-Kirl
    • Biomedical Science Letters
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    • v.8 no.4
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    • pp.223-228
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    • 2002
  • In vitro bioassays of aqueous extracts of 25 herbal species were performed by ovicidal activity against Strongvloides venuelensis. 500 mg of feces from Mongorian gerbil infected with S. venuelensis were placed into polyvinyl culture with extracts of herb and anthelmintic drugs in triplicates. Larval activity inside the polyvinyl bag was recorded microscopically at 12 hours, and every 24 hours thereafter for up to 72 hrs incubation. Eggs were not hatched within 72 hrs and degeneration was evident in the presence of extracts. The ovicidal activity characterized by the absence of L$_3$ in fecal cultures inside the polyvinyl bag. 1. Among 25 herbs, 12 herbs had ovicidal effects and caused degeneration of larvae in egg. S. angustifolia, I. helenium, A. korenum, P. tenuifolia, F. virridissima, A. asiatica, S. chinensis, A. tataricus, S. baicalensis, M. rimosa, S. glabra and Z. piperitum were the most ovicidal effect. However, P. thunbergiana, T. chinensis, C. flos, G. uralensis, C. obutusifolia, C. fortunei, A. koreana, P. grandiflorum, P. tenuifolia (Radix), P. mume and A. asphodelodes showed no ovicidal effects in vinyl bag culture. 2. Of 25 hrebs, 4 herbs had ovicidal and wormicidal effects, P. thunbergiana, C. flos, B. Striata and T. chinensis were characterized by degeneration of larvae.

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Ovicidal Effect of Plant Extract Mixture Against Seven Major Insect Pests (7종의 주요 해충에 대한 식물추출물의 살란 효과)

  • Hee-A Lee;Young Su Lee
    • Korean journal of applied entomology
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    • v.63 no.1
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    • pp.75-76
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    • 2024
  • The ovicidal effect of plant extract mixture (5%cinnamon extract + 10% citronella oil + 30% citrus oil + 10% derris extract + 20% neem extract + 25% penetrating surfactant) against several major insect pests was tested using the spraying method. In the case of stink bugs, eggs tended to die during hatching. When treated with a plant extract mixture (500-times solution), mortality for Halyomorpha halys, Riptortus clavatus, Eurydema dominulus, Trialeurodes vaprarorium, Bemisia tabaci, Spodoptera exigua, and Agrotis ipsilon reached as high as 100%. Therefore, it is believed that industrialization will be feasible in the future.

Development of a Bioassay Method Using Aluminium Foil Sheet for Screening Ovicidal Activity Against Diamondback Moth Egg, Plutella xylostella L. and Selection of Plant Extracts with High Ovicidal Activity (알루미늄호일 이용 배추좀나방(Plutella xylostella L.) 살란활성 검정법 개발 및 살란활성 식물추출물의 선발)

  • Kwon, Min;Kim, Ju-Il;Lee, Seung-Hwan;Lin, Mei-Ying
    • The Korean Journal of Pesticide Science
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    • v.15 no.2
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    • pp.194-200
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    • 2011
  • Diamondback moth (DBM, Plutella xylostella L.) is known as the most destructive pest of cruciferous crops worldwide. As most insecticides targeted to mainly larval stage, new insecticides which have hatching-inhibitory or ovicidal activity could be more efficient to control DBM. Therefore, we developed an easy and efficient method for screening ovicidal activity of DBM eggs using aluminum foil. The aluminum foil ($4{\times}12$ cm) coated with filtered juice of chinese cabbage leave (hereinafter called oviposition foil) exposed to 300 newly-emerged adults for 24 hours inside the rearing container. The oviposition foils were replaced every 4 days consecutively after mating, but it was better to discarded over then. Oviposition foil were divided into 6 to 12 pieces depending on egg mass volume. After dipping into test solutions for 10 seconds using faucet, oviposition foil pieces were placed into common petri dish, and then investigated hatchability. The effect of methanol solvent (50%) for 10 seconds dipping on the toxicity against DBM eggs was negligible. In addition, whether covering the petri dish or not should be dependent on nature of active compounds tested. With applying the new bioassay method, methanol extracts from 50 plants were tested the ovicidal activity to DBM eggs. Among them, four plant extracts; Angelica tenuissima root, Lycium chinense root, Cnidium officinale root and Polygala tenuifolia root, showed high ovicidal activity of over 90% control efficacy, against DBM eggs.

Acaricidal Activity of S-Aryl Arenethiosulfonates (S-Aryl Arenethiosulfonate 유도체의 살응애력과 살란력)

  • Lee, Sang-Ik;Park, Chang-Kyu;Kuem, Yuong-Soo
    • Applied Biological Chemistry
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    • v.40 no.3
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    • pp.249-253
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    • 1997
  • The acaricidal and ovicidal activity against red spider mite (Tetranychus urticae) was examined for a series of S-aryl arenethiosulfonates and compared with sulfonate acaricide, ovex and senile. Substituted diphenyl disulfides were obtained from substituted thiophenols, chloronitrobenzene and aniline derivatives. The S-aryl arenesulfonothioates were synthesised from the oxidation of disulfides. Three kind of para substituted compounds were found to have good ovicidal effect but acaricidal activity against adult mite was negligible in all S-aryl arenethiosulfonates.

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Susceptibility of greenhouse whitefly, Trialeurodes vaporariorum (Homoptera: Aleyrodidae) against commercially registered insecticides in Korea (국내등록사용중인 살충제에 대한 온실가루이의 감수성)

  • Kim, Chang-Woo;Kim, Jeong-Wha;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.4 no.3
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    • pp.75-81
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    • 2000
  • These studies were carried out to investigate the toxicities of 38 registered insecticides to the sweetpotato whitefly (Trialeurodes vaporariorum). Insecticide activities were evaluated by testing systemic action and residual effect in the laboratory, and control efficacy in the greenhouse. All experiments were tested at the recommended concentration(ppm) of each insecticides. Insect growth regulators (IGRs), only pyriproxyfen showed over 90% of ovicidal effect. The insecticides that showed over 90% of larvicidal activity oil 3rd nymphal instars were abamectin, acetamiprid, chlorpyrifos-methyl, imidacloprid, pyripoxyfen, and acetamiprid+ethofenprox. Insecticides with 100% adulticidal activity were abamectin, acephate, acetamiprid, benfurcarb, bifenthrin, furathiocarb, endosulfan, fenitrothion, imidacloprid, phenthoate, pymetrozine, acetamiprid + ethofenprox, ethofenprox + diazinon, furathiocarb + difluberlzuron, and triazamate+${\alpha}$-cypermethrin. Abamectin, acetamiprid, imidacloprid, pyriproxyfen, and acetamiprid + ethofenpox showed both residual effect and systemic activity. In tile control efficacy test on B. tabaci, 90% control values were obtained at 11th day after treatment of the insecticides including abamectin, acetamiprid, imidacloprid, pyripoxyfen and acetamiprid + ethofenprox. These results indicate that abamectin, acetamiprid, imidacloprid, pyriproxyfen and acetamiprid + ethofenprox can be used for tile control of B. tabaci in field.

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Effect of tebufenozide on embryonic and postembryonic development of sycamore lace bug, Corythucha ciliata(Hemiptera: Tingidae) (버즘나무방패벌레(Corythucha ciliata)의 배자발육과 후배자발육에 미치는 tebufenozide의 영향)

  • Choi, Mi-Hyun;Kim, Jeong-Wha;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.3 no.2
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    • pp.74-80
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    • 1999
  • These studies were carried out to investigate the effects of nonsteroidal ecdysone agonist, tebufenozide on embryonic and postembryonic development of sycamore lace bug, Corythucha ciliata. Tebufenozide exhibited ovicidal activity($LC_{50}=4.0$ ppm). Susceptibility of each instar nymphs to tebufenozide was nearly same($LC_{50}=2.3{\sim}6.0$ ppm). The tebufenozide treatment in the final instar affected the emergence, longevity and fecundity of surving adults significantly. Tebufenozide applied at high concentration (10 ppm) to younger adults delayed prioviposition period, and decreased adult longevity, the number of eggs laid per female and hatchability. The treatment also inhibited the ovarian development of the female adults.

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Comparative Toxicities of Selected Acaricides against the Twospotted Spider Mite(Tetranychus urticae Koch) to Establish the Screeing System for New Acaricidal Chemical Compounds (스크리닝체제 확립을 위한 점박이응애에 대한 몇가지 살비제의 약효 비교)

  • 조점래;최용호;박노중;조광연
    • Korean journal of applied entomology
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    • v.32 no.2
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    • pp.123-128
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    • 1993
  • The comparative toxicities of selected acaricides against the twospotted spider mite, Tetranychus urticae, were investigated. The ovicidal and adulticidal activities of selected acaricides were examined under different developmental stages. Fenpyroximate showed high activity throughout all developmental stages of mites. The oviposition inhibition effect of fenpyroximate was more highly effective than that of cyhexatin. At 100 ppm concentration, the effect of fenpyroximate on the residual oviposition inhibition was persistant during 25 days with 85% level, while the effect of cyhexatin gradually decreased, and then decreased to 40% at 25 days after treatment. Fenpyroximate at 100 ppm showed 100% knockdown activity within 3 hour while cyhexatin showed only 91% knockdown activity within 24 hours after treatment. Most of selected acaricides had no systemic activity, but metasystox at 800 ppm had 100% of the systemic activity at 24 hours after treatment. For the influence of temperature on the activity, fenpyroximate showed stable activity and no temperature-dependent in comparison with other selected adaricides.

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Toxicities and Control Effect of Three Insecticides to Greenhouse Whitefly, Trialeurodes vaporariorum and Sweetpotato Whitefly Bemisia tabaci (Homoptera: Aleyrodidae) (몇 가지 살충제의 온실가루이와 담배가루이의 생육 단계별 살충효과 및 방제효과)

  • Ha, Tae-Ki;Hwang, In-Cheon;Kim, Jong-Kwan;Song, Yoo-Han;Kim, Gil-Hah;Yu, Yong-Man
    • The Korean Journal of Pesticide Science
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    • v.7 no.3
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    • pp.207-215
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    • 2003
  • This study was carried out to evaluate toxicities of 3 registered insecticides to greenhouse whitefly(GWF), Trialeurodes vaporariorum and sweetpotato whitefly(SWF), Bemisia tabaci, B-biotype. Insecticide activities were evaluated by testing systemic action, residual effect in the laboratory, and control efficacy in the greenhouse. All experiments were tested at the recommended concentration(RC), half and a quarter concentrations of RC of each insecticides. Acetamiprid showed 45%, 42% ovicidal effect to greenhouse whitefly and sweetpotato whitefly at 40 ppm, respectively. Acetamiprid showed more than 97% larvicidal activities on the 3rd instars larvae of GWF and SWF at the recommended and its half concentrations. On the adults of the two whitefly species, acetamiprid and acetamiprid+ethofenprox showed more than 92% mortality even at half of recommended concentrations. Acetamiprid and acetamiprid+ethofenprox showed both residual effect and systemic activity. In the control efficacy test on GWF and SWF, 90% control values were obtained at the 3th day after treatments of acetamiprid and acetamiprid + ethofenprox by application with recommended concentration. These results indicate that acetamiprid and acetamiprid+ethofenprox can be used in the control of the two whitefly species in field.

Effect of COY(Cooking Oil and Yolk Mixture) on Control of Tetranychus urticae (난황유의 점박이응애(Tetranychus urticae) 방제효과)

  • Park, Jong-Ho;Ryu, Kyoung-Yul;Lee, Byung-Mo;Jee, Hyeong-Jin
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.249-254
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    • 2008
  • This study was conducted to develop an organic control method of two spotted spider mite(Tetranychus urticae) by using cooking oil and yolk mixture(COY) through evaluating its acaricidal activity in laboratory and green house. In laboratory, there is no significant difference in acaricidal activity against T. urticae within the COY including soybean, canola(rape seed), sunflower and olive oil. The acaricidal activity against T. urticae. increased from 17.6% to 94.1% as the COY became concentrated between 0.1% to 1%. The COY acricidal activity was effected by the quantity of treatment according to application methods. The COY dealt with T. urticae eggs presented 95% of the ovicidal activity. In rose greenhouse damaged by T. urticae, the COY(0.3%) was sprayed three times and resulted in the high control value of mites between 69.0% to 89.6%.

Susceptibility of Embryonic and Postembryonic Developmental Stages of Riptortus clavatus(Hemiptera : Alydidae) to Diflubenzuron (톱다리개미허리노린재의 배자발육에 미치는 Diflubenzuron의 영향)

  • 안용준;김길하;조광연
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.480-485
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    • 1992
  • Laboratory studies were done to evaluate the effects of diflubenzuron on embryonic and postembryonic development of Riptortus clavatus Thunberg. Diflubenzuron prevented egg hatch; younger eggs(O-12 h old) were 2 times as susceptible as older(48-60 h old) eggs, but embryos of both younger and older eggs developed normally. Susceptibility of nymphs to diflubenzuron decreased with each successive molt. Compared with the first instar, relative tolerance to diflubenzuron was 1.5 times for the second instar, 18.2 times for the third instar, 39. 4 times for the fourth ins tar and 42.4 times for the fifth instar. Even as low concentrations, diflubenzuron prevented significant numbers of third instar nymphs from developing to fourth and fifth instar nymphs or adults. Weight, longevity and fecundity of adults surviving treatment in the final (fifth) instar were also adversely affected.

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