• Journal of Nutrition and Health
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• 제36권2호
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• pp.167-174
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• 2003

This study investigated associations between nutrient intake, lumbar bone mineral density (BMD) , and bone mineral content (BMC) among 33 ovariectomized women (mean age =47.2 y) . Forty-five premenopausal women participated as a control group. The BMD and BMC of the lumbar spine (L$_2$-L$_4$) were measured by dual energy x-ray absorptiometry. Nutrient intake was estimated by the convenient method and a quantitative food frequency questionnaire was designed for this study that included the most commonly consumed foods sources of calcium. Participants were asked to identify all daily physical activities, and the number of hours per activity. The participants were also grouped by calcium intake. The total calcium intake of all participants was estimated by dietary calcium intake and then the subjects were divided into quartiles to assess the lumbar BMD and BMC of the highest quartile and the lowest quartile of calcium intake. The ovariectomized women consumed 602 mg/d of calcium which is 86% of RDA. There were significant differences in lumbar BMD and BMC between control and ovariectomized group. Within ovariectomized group the highest quartile calcium intake group had significantly greater lumbar bone mineral density and bone mineral content than the lowest quartile calcium intake group. Correlation analysis revealed that the ALP was positively associated with calcium index in control women, while ALP was positively associated with energy intake in ovariectomized women. And body weight was positively correlated with the spinal BMD and BMC in all women. The spinal BMD was negatively associated with menarche age, number of child, and the age of last child delivery, and age in control women. However, neither menarche age nor the age of last child delivery were associated with both spinal BMD in ovariectomized women. These results confirmed that ovariectomized and low calcium intake is associated with poor bone mineral density. Energy and calcium intake and adequate body weight should be recommended in ovariectomized women to prevent osteoporosis.

• 동아시아식생활학회지
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• 제27권4호
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• pp.442-449
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• 2017

Adenophora triphylla var. japonica extract has been reported to have estrogenic activity and beneficial effects in postmenopausal women requiring estrogen replacement therapy. This study investigated the effects of A. triphylla extract on cognitive function in ovariectomized mice. Water extracts from A. triphylla were administrated to ovariectomized mice fed a high fat diet for 6 weeks. The step through latency time in the passive avoidance test was shortened in ovariectomized mice as compared with sham-operated mice. However, administration of A. triphylla extracts had a tendency to prolong the latency time in ovariectomized mice. Moreover, A. triphylla supplementation caused a significant decrease in mean escape latency in the water maze test. These results indicate that A. triphylla could be used to improve estrogen deficiency-related learning and memory impairments in postmenopausal women.

• Journal of Nutrition and Health
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• 제31권4호
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• pp.716-728
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• 1998

To investigate the effect of dietary Ca levels on metabolic changes of Ca and skeleton in postmenopausal women, 10-month-old ovariectomized female rats were compared with 2 month old rats. The rats were fed either 0.2% or 1.2% Ca diets for 16 weeks. Food intake and weight gain as higher in rats fed high Ca diets and in ovariectomized rats. Apparent Ca absorption as higher, and Ca balance was lower in the low Ca groups. Vertebrae density was higher in old rats or those fed a high Ca diets. The old rats and ovariectomized rats showed decreased bone formation, increased bone resorption and kidney function deterioration resulting in increased urinary Ca excretion. Contradictory to the above observation, old rats and ovariectomized rats still showed higher bone mass and bone ash content. Therefore aging was not fully onging in 10-month-old rats. Bone weights, mineral contents, and mineral/wt ratio were lower in ovariectomized rats. Dietary Ca level did not affect urinary Ca excretion, urinary protein excretion, GFR, serum alkaline phosphatase, or urinary hydroxyporline excretion. This means that dietary Ca level did not influence kidney function or bone turnover. However Ca content and the ash content of femur, 4th vertebra, and scapula were increased in high Ca groups. Therefore, it is considered that decreased bone formation and accelerated bone resorption may account for the increased osteoporotic risk in women in menopause after middle age. However, Ca metabolism can be improved and bone components can be maintained if Ca is supplemented.

• 대한의생명과학회지
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• 제28권2호
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• pp.101-108
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• 2022

High-fat diet (HFD)-fed ovariectomized (OVX) female mice were used as an animal model of obese postmenopausal women. We investigated the effects of ascorbic acid on the histological changes induced in the liver. Plasma alanine aminotransferase levels and liver weights were higher in mice fed an HFD for 18 weeks than in mice fed a low-fat diet, effects that were inhibited by ascorbic acid. Similarly, mice fed an ascorbic acid-supplemented HFD had less hepatic lipid accumulation than did mice fed an HFD alone. Moreover, administration of ascorbic acid reduced inflammatory cells, including mast cells and CD68-positive cells, and inflammatory foci in the liver and inhibited hepatocyte ballooning. Hepatic collagen levels were lower in ascorbic acid-treated versus non-treated mice. These results suggest that ascorbic acid inhibits hepatic steatosis, inflammation, and fibrosis in obese OVX mice. Thus, ascorbic acid intake may be useful for postmenopausal women with nonalcoholic fatty liver disease.

• 생명과학회지
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• 제15권6호
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• pp.1028-1033
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• 2005

여성의 폐경에 의한 eskogen의 감소는 심혈관계질환을 빠르게 진행시킨다고 알려져 있다. 최근 새로운 식품으로 각광받고 있는 매생이 추출물을 갱년기장애를 일으킨 횐쥐에 투여하여 혈 중 지질 수준변화와 항 혈소판 응집 능을 통해서 그 효과를 검토해 보았다. 매생이 추출물을 갱년기를 유도한 횐쥐에 투여하여 혈소판 응집 능에 미치는 영향을 검토한 결과 난소절제에 의하여 혈소판응집이 활성화되었는데, 매생이 추출물을 투여한 군에서 혈소판응집이 억제되는 것을 확인할 수 있었다. 또한 난소 절제에 의해 total-cholesterol의 함량이 증가되었는데, 이는 매생이 추출물을 투여함으로써 감소하는 경향을 보였다. 혈 중 중성 지방에서는 매생이을 투여한 모든 군에서 난소를 절제하지 않는 군과 비슷한 함량으로 감소하였고, 또한 혈중 HDL-cholesterol은 매생이 추출물 모두 난소 절제한 군과 비교해서 높은 경향을 나타내어 매생이 추출물이 혈액과 관련된 심혈관계 질환에 유효한 효과를 나타낼 것으로 사료된다.

• 동의생리병리학회지
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• 제21권1호
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• pp.111-116
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• 2007

The aim of this study was to evaluate the effect of Lycii fructus (LF) on serum lipid and the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rat (Sham), ovariectomized control rat (OVX-control), and ovariectomized rats supplemented with the LF 50mg/kg bw/day (OVX-LF). The LF ethanol extract were orally administrated 1ml per day. Body weight gain was not significantly different in groups. Although total-cholesterol and triglyceride were increased in the ovariectomized control, supplementation with the LF extract decreased the levels. Moreover, the serum HDL-cholesterol levels were significantly increased after supplementation with the LF extract(p<0.05). Supplementation with the LF extract prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of LF. Therefore, it may be used to possibly improve the quality of life in menopausal women.

• 생약학회지
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• 제37권4호
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• pp.324-330
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• 2006

The aim of this study was to evaluate the effects of Artemisia Princeps Var. Orientalis (AP) on serum lipids and the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with the AP 50 mg/kg bw/day (OVX-AP). The AP ethanol extracts were orally administrated 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats at all time points, but supplementation with the AP extracts tended to gain weight less than OVX-control. Although total-cholesterol was increased at OVX-control, supplementation with the AP extracts tended to result in less than OVX-control. Triglyceride was significantly decreased after supplemented with the AP extracts (p<0.05). HDL-cholesterol is appeared higher AP extracts group than OVX-control. According to the results, we could know the fact that AP extracts were effective on serum lipids content throughout decreasing total-cholesterol, triglyceride and increasing HDL-cholesterol in ovariectomized rats. Supplementation with the AP extracts prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of AP. Therefore, it may be used to possibly improve the quality of life in menopausal women.

• 한국약용작물학회지
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• 제20권1호
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• pp.42-46
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• 2012

Soy isoflavones have been suggested to improve bone loss and lipid profile in postmenopausal women and ovariectomized rats. In present study, we investigated the hypothesis that consumption of soybean, mung bean, cowpea and azuki bean has a beneficial effect on lipid profile which associates with bone mass in ovariectomized rats. Forty two female Sprague-Dawley rats were either sham-operated (Sham) or surgically ovariectomized (OVX). Sham and OVX groups were fed a regular AIN-93M diet, but ovariectomized rats with soybean (OS), mung bean (OM), cowpea (OC) or adzuki bean (OA) were fed AIN-93M diet replacing 35% of corn starch with powdered OS, OM, OC or OA for 10 weeks. Total- and LDL-cholesterol concentrations were significantly lower in Sham and OC groups than other OVX groups. There was significant negative association between total cholesterol concentration and bone mineral density (BMD) of tibia in only OC group. In conclusion, total-cholesterol concentration was significantly and negatively correlated with BMD in rats consumed cowpea, suggesting that the reduced cholesterol concentration may have a beneficial effect on bone mass.

• 생명과학회지
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• 제16권7호
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• pp.1219-1224
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• 2006

여성의 폐경에 의한 estrogen의 감소는 골다공증을 유발시킨다고 알려져 있다. 최근 새로운 식품으로 각광받고 있는 매생이 추출물을 인위적 갱년기 장애를 유발시킨 흰쥐에 투여하여 혈 중 ALP활성 변화와 골 중의 collagen의 함량 및 collagen 가교물질 pyridinoline과 deoxypyridinoline 함량 변화를 측정하여 그 효과를 검토해 보았다. 매생이 추출물을 갱년기를 유도한 흰쥐에 투여하여 혈 중 ALP활성 변화에 미치는 영향을 검토한 결과 난소절제에 의하여 ALP활성이 증가하였으나, 매생이 추출물 투여한 군에서 ALP활성이 억제되는 것을 확인할 수 있었다. 또한 난소절제에 의해 연골의 collagen 함량이 감소되었는데, 이는 매생이 추출물을 투여함으로써 정상적인 수치로 증가하는 경향을 나타내어 매생이 추출물이 골 대사와 관련된 골 대사질환에 유효한 효과를 나타낼 것으로 추측된다. 또한 collagen 가교물질로써 골대사의 biomaker인 pyridinoline 및 deoxypyridinoline의 연골 중의 함량은 난소절제에 의해 감소하였고, 매생이 추출물 투여에 의해 증가하여 estrogen부족으로 인한 골 손실에 매생이 추출물이 유익한 효과를 가지는 것으로 보이며, 이는 매생이 중에 함유되어 있는 phytoestrogen에 의한 것으로 추측되어 앞으로 구체적인 검토가 필요한 것으로 사료된다.

• 한국식품영양과학회지
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• 제33권2호
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• pp.324-330
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• 2004

난소 절제시술 및 폐경으로 인한 여성 호르몬인 estrogen의 감소는 지속되는 골 손실 가속화 등으로 인한 골 질환의 문제점을 가지고 있다. 그러므로 본 연구에서 흰쥐의 난소를 절제하여 인위적인 폐경을 유도하여 골손실의 정도를 결합조직 중의 collagen 함량 및 collagen성숙가교인 pyridinoline함량을 측정하여 그 효과를 검토하였다 난소 절제 후 결합조직 중의 collagen함량은 연골과 피부에서 난소를 절제하지 않은 Sham군에 비하여 낮았으며, 김 추출물 투여군에서 유의적이지는 않으나 높은 경향을 나타내었다. 골 중 collagen성숙가교인 pyrldinoline 함량은 난소절제에 의해 감소하였으나 골에서는 김 추출물 투여에 의해 난소를 절제하지 않은 Sham군과 비슷한 수준으로 증가하였고, 연골에서는 김 추출물 투여에 의해 Sham군과 비슷한 수준으로 감소하는 경향을 나타내어 pyridinoline 생성에 유익한 효과를 나타내었다. 김 추출물이 collagen합성 및 성숙가교인 pyridinoline 생성을 회복시킨 본 실험의 결과로 보아 폐경기여성에게 김 추출물의 섭취는 폐경으로 인한 골손실을 감소시켜 줄 것으로 사료되어지며, 이러한 작용을 나타낸 김의 성분 및 그 기전에 대해서는 앞으로 더 많은 연구가 필요할것으로 생각된다.