• Reproductive and Developmental Biology
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• 제39권4호
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• pp.127-132
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• 2015

To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred ${\alpha}$-1,3-galactosyltransferase knock-out ($GalT^{-/-}$) pigs. In this study, the somatic cells and tissues from the $GalT^{-/-}$ pigs were characterized by an analysis of the expression of Gal${\alpha}$-1,3-Gal (${\alpha}-Gal$) epitope. Briefly, ear fibroblast cell lines of 19 homozygous $GalT^{-/-}$ pigs were established and cryopreserved. The expression of ${\alpha}-Gal$ epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous ($GalT^{-/-}$) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of ${\alpha}-Gal$ epitope expression. The results showed that the expression of ${\alpha}-Gal$ epitope in $GalT^{-/-}$ cells (0.2 %) were significantly (p<0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous ($GalT^{-/+}$) (9.3 %) and wild type ($GalT^{+/+}$) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of ${\alpha}-Gal$ epitope was detected a partly in $GalT^{-/+}$ cells and mostly in $GalT^{+/+}$ cells, it was almost not detected in the $GalT^{-/-}$ cells. Also, immunostaining results from various tissues of the $GalT^{-/-}$ pig showed that the expression of ${\alpha}-Gal$ epitope was not detectable, whereas various tissues from $GalT^{+/+}$ pig showed a strong expression of ${\alpha}-Gal$ epitope. Our results demonstrated that ${\alpha}-Gal$ epitope expressions from $GalT^{-/-}$ pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.

• 한국수정란이식학회지
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• 제32권3호
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• pp.87-94
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• 2017

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at $37^{\circ}C$. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

• 멤브레인
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• 제19권4호
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• pp.317-323
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• 2009

본 연구는 전자 및 반도체 산업의 각종 에칭공정에서 발생되는 중금속 함유 산성 폐에칭액을 NF 막분리법을 이용하여 에칭액 회수와 중금속 처리를 효율적으로 수행하기 위한 NF 막공정의 운전조건을 설정하기 위한 기본 자료를 확보하는데 있다. 이를 위해 3가지 종류의 상용 NF 막(General Electric Co. Duraslick NF-4040 막, Dow Co. Filmtec LP-4040 막 및 Koch Co. SelRO MPS-34 4040 막)을 대상으로 $Pb^{+2}$ 중금속을 함유한 모의 질산 폐에칭액의 회분식(dead-end) 막여과 실험을 수행하여 폐에칭액의 투과 플럭스와 $Pb^{+2}$ 중금속 이온의 총괄 배제도를 측정하여 폐에칭액 처리에 우수한 NF 막을 선정하였다. 실험결과 질산용액에의 막 보관기간이 길수록, 질산용액의 pH가 낮을수록 산에 의한 막의 손상이 심해졌으며, 질산에 의한 막의 손상은 SelRO MPS-34 4040 < Duraslick NF-4040 < Filmtec LP-4040 막의 순서로 심하게 일어났다. 또한 질산용액에의 막 보관기간이 길수록, 질산용액의 pH가 낮을수록 $Pb^{+2}$ 이온의 배제도가 낮아졌으며, 배제도 값은 Duraslick NF-4040 막의 경우에는 95% 수준의 초기 배제도 값에서 질산용액에의 4달 보관 후에는 20% 수준으로, SelRO MPS-34 4040 막의 경우에는 초기 85% 수준에서 4달 후 65% 수준으로, Filmtec LP-4040 막의 경우에는 초기 90% 수준에서 4달 후 10% 이하 수준으로까지 감소하였다. 3종류의 상용 NF 막 중 내산성 용도로 개발된 SelRO MPS-34 4040 막이 중금속 함유 산성 폐에칭액의 재생에 가장 적합하였다.

• 오토저널
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• 제2권1호
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• pp.39-50
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• 1980

This paper presents the variations obtained in heat flow rate and engine performance of a four-stroke cycle Diesel engine when there were changes in the temperature of cooling water, compression ratio, injection timing of fuel, and other factors. Heat dissipation of engine cylinder was calculated by the heat transfer coefficient of Nusselt's empirical equation and the analysis of distribution of temperature in cylinder barrel was obtained by the finite element method of two-dimensional steady state heat conduction. In this experiment, the out side temperature of cylinder liner was measured by the data logger, and the temperature distribution of liner was computed by the analysis of triangular finite element model under the assumption due to surface heat flux of cylinder inner surface. The results obtained by this study are as follows. Under the given operating condition, the temperature distribution of cylinder liner by using finite element method shows that the mean temperature of barrel is in accordance with the experimental results of Eichelberg and temperature difference is lower than 4.23.deg. C. The heat dissipation of engine decrease in accordance with the decrease of piston mean velocity, compression ratio, and the increase of coolant temperature. Influence on the delay of injection timing of fuel brings about the decrease of heat rejection over the cylinder at constant test conditions.

• 패션비즈니스
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• 제7권6호
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• pp.10-20
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• 2003

The object of this research is to develop the designs with aesthetics and function for making the uniforms of specialty restaurants of Korean dishes in pursuit of the image of excellent dignity and its result is as follows: As for designs, this research chose the traditional image as the basic concept and made visual Korean lines, colors, and patterns. As for lines, it made visual the curve of the eaves, the straight line of polls, and the fret of windows and doors represented in architecture and applied them, as for color tones, it chose traditional 'Obangsaek', five direction colors. As for the patterns, it symbolized 4 trigrams( Geon, Gon, Gam, and Yi), the cloud pattern, also it tried to get the formative beauty from traditional patchwork wrapping cloth and windows and doors. The expectant effects on the design of Uniform are as follows: First, it offered basic clothes for male and female employees working in the hall and suggested two kinds of skirt and pants for the latter. It tried to find out both the function of pants and the female beauty of skirts by wrapping on pants to eliminate the feeling of rejection towards the style of them, the use of which have been recognized for man only in spite of many merits of them. Second, it sought for the characteristics of shape on collar, breast-tie, and fold etc. of Korean clothes and designed clothes according to each employee's role and finally emphasized their traditional aesthetics.

• Asia Marketing Journal
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• 제11권2호
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• pp.97-119
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• 2009

The growth of international trade and the formation of supranational economic and political trading blocks have noticeably widened the presence on the market of products of different national origins. This has stimulated interest in explaining the Country-of-Origin (COO) role in domestic and international markets and its consequences on consumer behaviour. Since the consumer purchasing decisions can be decisive to the success of a company's strategy in domestic and foreign markets, the objective of this study is to present empirical evidence on the extent to which reputation of firms associated to a certain COO are related to consumer purchase intention. Additionally our study considers ethnocentrism as a variable that partially explains the rejection of imports products based on its foreign origin. The empirical application of the proposed model is related to the purchase of Korean automobiles which represents 5.7% of the national market share in Spain. Structural equation modelling was used to analyse the data collected from 202 personal interviews carried out in a large Spanish region. The results show that reputation of firms associated to a certain COO in an important factor to establish business relationships involving consumers and firms from different countries and increase intentions to purchase Korean products. Additionally, ethnocentric consumers prefer to purchase domestic products rather than foreign imports as an attempt to protect national economy however the negative effect of ethnocentrism is weaker than positive effect of firms reputation of a COO.

• Journal of the Optical Society of Korea
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• 제16권3호
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• pp.288-291
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• 2012

We designed an ultra-wide band-pass filter applicable to microwave reflectometry for KSTAR (Korea Superconducting Tokamak Advanced Research) and to microwave photonics. The proposed ultra-wide band-pass filter exhibits a metamaterial structure characterized by a wide band, low insertion loss, and high skirt selectivity. The proposed filter is applied to enhance the linearity of reflectometry at the output of a VCO (voltage controlled oscillator). The pass-band of the proposed filter is observed at 18~28 GHz, and the out-of-band rejection is below 20 dB. Further, we constructed an unwrapped negative phase of S(2, 1) to verify the characteristics of the metamaterial. The under- and upper-band at lower limits of the pass-band are left- and right-handed, respectively. The group delay of the filter is less than 0.5 ns.

• 보건의료산업학회지
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• 제4권2호
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• pp.107-117
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• 2010

In this paper, the researcher looked into major issues in the "Act (Draft) on Remedy for Damage from Medical Accident and Medical Dispute Mediation, etc." which was proposed by the Health & Welfare Committee, the National Assembly of the Republic of Korea, and which was pending with the Legislation & Judiciary Committee. Then the researcher pointed out worrisome problems therein and presented suggestion" to improve problematic situations. First of all, the researcher examined the following items which are major points in the aforementioned Act: 1) Establishment of Korea Medical Dispute Mediation and Arbitration Center, 2) Procedures for mediation and arbitration of medical disputes, 3) Establishment of Medical Injury Compensation Association, 4) Introduction of proxy payment for damages, 5) Compensation for no-fault medical accidents, 6) A system concerned with special cases on criminal punishment. Next, the researcher closely reviewed the following possible issues: 1) Limit of arbitrary mediation, 2) Postponement of the system concerned with special case on criminal punishment, 3) Examination of reasons for rejection, 4) Function and role of the Appraisal department, 5) A possibility of being reduced to an evidence collection procedure for lawsuit, 6) A possibility of no-fault compensation rather than injury compensation, 7) Operational issues related proxy payment for damages. Lastly, the researcher presented suggestions on how to improve each problematic issue.

• 정보학연구
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• 제2권2호
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• pp.139-144
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• 1999

본 논문은 음성인식 시스템에서 정확도를 높이기 위해 후처리 단계에서 후보 단어들의 엔트로피 정보를 이용하였다. 기존의 우도비 검출방법은 음성 데이터에 따라 음성인식 시스템의 성능이 변하고 N개의 후보단어들의 우도값이 비슷하여 오인식 발생확률이 높았다. 그러나 본 눈문에서는 각 후보 단어들의 엔트로피 값보다 인식대상 단어 외의 단어들의 엔트로피 값이 상대적으로 낮은 후보를 거절하는 후처리 방법을 사용하여 음성 데이터에 독립적이면서도 변별력을 높인 정확한 음성인식 시스템을 얻을 수 있었다. 실험 결과 본 논문에서 제안하는 엔트로피에 의한 후처리 방법은 우도비에 의한 방법보다 인식 시스템의 성능을 false alarm이 20%일 때 최대 3.6% 향상시킬 수 있었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제8권2호
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• pp.36-43
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• 2003

질산성 질소 제거를 위해 미셀 형성을 이용한 한외여과(MEUF)공정의 타당성을 양이온성 계면활성제인 octadecylamine acetate(ODA)와 cetylpyrinidium chloride(CPC)를 사용하여 조사하였다. 3 몰비의 계면활성제를 가지고 최소 80%의 질산성 질소를 제거할 수 있었으며, 10 몰비의 계면활성제로는 98％ 이상의 제거율을 얻을 수 있었다. ODA가 CPC 보다 높은 질산성 질소와 계면활성제 제거율을 보였으며, 이는 계면활성제 구조상 ODA의 머리부분이 CPC의 머리부분보다 질산성 질소의 접근이 용이하기 때문이다. MEUF공정은 질산성 질소를 효과적으로 제거할 수 있으며, CPC보다 ODA가 질산성 질소를 제거하기 위해 더 바람직한 계면활성제이다.