• 한국환경과학회지
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• 제25권1호
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• pp.173-179
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• 2016

Leaf water and osmotic potential, chlorophyll content, photosynthetic rate, and electrolyte leakage were measured to evaluate tolerance to water stress in wild-type (WT) and transgenic tobacco plants (TR) expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts. Leaf water potential of both WT and TR plants decreased similarly under water stress condition. However, leaf osmotic potential of TR plants more negatively decreased in the process of dehydration, compared with WT plants, suggesting osmotic adjustment. Stomatal conductance (Gs) in WT plants markedly decreased from the Day 4 after withholding water, while that in TR plants retained relatively high values. Relatively low chlorophyll content and photosynthetic rate under water stress were shown in WT plants since $4^{th}$ day after treatment. In particular, damage indicated by electrolyte leakage during water stress was higher in WT plants than in TR plants. On the other hand, SOD and APX activity was remarkably higher in TR plants. These results indicate that transgenic tobacco plants expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts improve tolerance to water stress.

• 농업과학연구
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• 제12권1호
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• pp.17-21
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• 1985

고추종자(種子)의 발아(發芽)와 출아기간(出芽期間)을 단축(短縮)시키고자 PEG 6000(polyethylene glycol 6000) 20, 25, 30% 용액(溶液)에 공시품종(供試品種)인 새로나와 킹건고추종자(種子)를 6일(日)과 12일간(日間) 침지처리(浸漬處理)하여 고추종자(種子)의 발아(發芽)와 출아촉진(出芽促進)에 미치는 효과(效果)를 조사(調査)하였다. 1. 새로나와 킹건고추는 PEG 20%와 25% 용액(溶液)에 12일간(日間) 종자(種子)를 침지(浸漬)하므로써 발아속도(發芽速度)가 촉진(促進)되었으며 최종발아율(最終發芽率)은 처리간(處理間)에 큰 차이(差異)를 인정(認定)할 수 없었다. 2. PEG 용액(溶液)에 침지(浸漬)한 종자(種子)를 건조(乾操)한 다음 파종(播種)한 경우 PEG 25% 용액(溶液)에 12일간(日間) 침지(浸漬)하므로써 새로나품종(品種)은 약(約)5일(日), 킹건고추는 약(約)4일(日)씩 출아속도(出芽速度)가 촉진(促進)되었다. PEG 처리(處理)에 따른 최종출아율(最終出芽率)은 새로나품종(品種)에서 증가(增加)된 반면 킹건고추에서는 처리간(處理間)에 차이(差異)가 인정(認定)되지 않았다. 3. 따라서 고추종자9種子)를 PEG20, 25% 용액(溶液)에 6일(日) 또는 12일간(日間) 침지처리(浸漬處理)한 후(後) 파종(播種)하므로써 불량(不良)한 저온환경조건(低溫環境條件)에서도 발아(發芽) 및 출아속도(出芽速度)를 촉진(促進)시킬 수 있을 것으로 사료(思料)된다.

• 미생물학회지
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• 제24권2호
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• pp.154-160
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• 1986

Cellulose분해력이 높은 Cellulomonas속 세균의 원형질체 융합을 위한 원형질체 형성조건을 조사하여 본 결과 같은 Cellulomonas 속인 Cellulomonas sp. C S 1- 1과 Cellulomonas flavigena NCIB 12901 균주일지라도 그 형성조건에 현저한 차이가 있어 CS1-l균주는 lysozyme농도 $100{\mu}g/ml$ 30분정도의 처리로 99.9%의 높은 원형질체 형성을 얻을 수 있는 반면 C.flavigena 균주는 lysozyme 농도도 훨씬 높고 장시간이 소요되어 $600{\mu}g/ml$. 6시간 정도의 처리에 약80%의 원형질체 형성을 보였다. 또한 세균 배양기간이 미치는 영향도 CS1-l균주는 별 영향을 받지 않고 원형질체화 되었으나 C. flavigena균주는 매우 민감하게 영향을 받아 대수증식기 중기의 세포가 말기의 세포보다 원형 질체가 잘 이루어 졌다. 원형질체 형성확인방법에서도 CS-1 균주는 osmotic shock을 주어 원형질체를 계수하거나 SEM으로 확인하거나 같은 결과를 얻었으나 C. flavigena 균주는 osmotic shock에 의한 원형질체의 계수결과와 SEM으로의 결과가 서로 달라 확인방법으로 두 방법이 병행되어져야 함을 보여주었다.

• 한국식품영양과학회지
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• 제33권9호
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• pp.1573-1579
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• 2004

단호박을 이용하여 새로운 가공제품을 개발하고자 여러가지 잇점을 줄 수 있는 삼투건조를 최적조건에서 처리하고 산업적으로 많이 사용되고 있는 열풍, 진공건조와 새로운 건조방법의 적용을 위해 선정한 냉풍건조를 행한 후 품질특성을 비교 평가하였다. 삼투처리 후 열풍, 진공, 냉풍건조된 단호박의 수분함량은 sucrose로 삼투처리한 경우가 모든 건조 방법에서 glucose 처리구보다 낮았으며, 건조방법에 따른 비타민 C의 함량은 glucose로 삼투처리 후 냉풍건조한 경우가 35.69 ㎎/100 g으로 가장 높게 측정되었다. 전반적으로 침지 용액에 따른 L, a, b값은 유사한 경향이었으며, 냉풍건조가 색도변화가 가장 작게 일어나 생단호박의 색상과 유사하였다. 건조 단호박의 표면구조는 삼투용액과 건조방법에 따라 상이하였는데, 삼투처리 후 냉풍건조된 단호박을 제외하고 열풍 및 진공건조된 단호박의 조직 손상이 크게 나타났다. 건조방법 및 건조시간에 따른 수분함량과 비타민 C함량을 살펴보면 수분함량의 경우 건조효율성을 고려할 때 sucrose로 삼투처리 후 진공건조한 단호박이 가장 우수하였으나, 비타민 C함량에서는 glucose로 삼투처리 후 냉풍건조한 단호박이 가장 양호하였다. 삼투용액에 따른 건조방법별 건조속도는 sucrose로 삼투처리한 단호박의 건조속도가 glucose 처리구보다 빠름을 알 수 있었다.

• 한국물환경학회지
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• 제33권4호
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• pp.403-408
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• 2017

In these days, wastewater reclamation and seawater desalination play essential role in addressing the challenge of worldwide water scarcity. Particularly, reverse osmosis (RO) for seawater desalination process is commonly used due to less energy consumption than conventional thermodynamic systems. However, membrane fouling and electrical energy consumption during operation of RO system for seawater desalination haver continued to be a obstruction to its application. In this study, therefore, wastewater secondary effluent is used for osmotic dilution of seawater. Firstly, fouling behaviour of RO by simulating wastewater effluent in osmotic dilution process was measured and we calculated energy consumption of overall desalination process by theoretical equations and commercial program. Our results reveal that RO membrane fouling can be efficiently controlled by pre-treatment systems such as nano filtration (NF) or forward osmosis (FO) process. Especially FO system for osmotic dilution process is a non-pressurized membrane system and, therefore, the operating energy consumption of overall desalination system was the lowest. Moreover, fouling layer on FO membrane is comparatively weak and reversible enough to be disrupted by physical cleaning. Thus, RO system with low salinity feed water through FO process is possible as a less energy consuming desalination system with efficient membrane fouling control.

• 산업식품공학
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• 제13권2호
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• pp.130-137
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• 2009

This study evaluated the potential of utilizing the osmotic solution from dried mango processing as alternative raw material for mango wine making. Fermentation was carried out using two kinds of yeast strains Saccharomyces bayanus, Lalvin EC-1118 and Saccharomyces cerevisiae, Lalvin D-47 at 20$^{\circ}C$ for 28 days. Physicochemical analysis during fermentation was performed for each treatment and the resulting wine samples were analyzed for color, volatiles and sensory properties. Results of physicochemical analysis between the two fermenting samples as well as the wine samples show almost similar results regardless of the yeast strains. Wine color of sample wines after storage were not significantly different at p<0.05 and when compared with a commercial mango wine. From the volatile analysis, esters and alcohols constituted majority of the compounds. Production of several esters, alcohols, acids and terpenes were affected by yeast strain used in fermentation. Results of sensory analysis showed that wines fermented by S. bayanus EC-1118 strain was more acceptable although sensory scores between the treatments and the reference wine showed significant differences in all the attributes evaluated, except for bitterness. The utilization of osmotic solution from dried mango process could produce similar properties with existing commercial mango wines although there is still need for further work on the improvement of some sensory attributes of the mango wines.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.60-69
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• 2007

Ruminants eating dry forage secrete large volumes of saliva which results in decreased plasma volume (hypovolemia) and the loss of $NaHCO_3$ from the blood. The present research investigated whether or not hypovolemia and the loss of $NaHCO_3$ from the blood in goats brought about by dry forage feeding actually depresses feed intake and saliva secretion, respectively. The present experiment consisted of three treatments (NI, ASI, MI). In the control treatment (NI), a solution was not infused. In the ASI treatment, i.v. infusion of artificial parotid saliva was initiated 1 h before feeding and continued for the entire 2 h feeding period. In the MI treatment, iso-osmotic mannitol solution was infused. The NI treatment showed that hematocrit and plasma total protein concentration were increased due to decreased circulating plasma volume brought about by feeding. In the ASI treatment, the fluid and $NaHCO_3$ that were lost from the blood because of a feeding-induced acceleration of saliva secretion was replenished with an intravenous infusion of artificial parotid saliva. This replenishment lessened the levels of suppression on both feeding and parotid saliva secretion. When only the lost fluid was replenished with an intravenous infusion of iso-osmotic mannitol solution in the MI treatment, the degree of feeding suppression was lessened but the level of saliva secretion suppression was not affected. These results indicate that the marked suppression of feed intake during the initial stages of dry forage feeding was caused by a feeding-induced hypovolemia while the suppression of saliva secretion was brought about by the loss of $NaHCO_3$ from the blood due to increased saliva secretion during the initial stages of feeding.

• Food Science and Biotechnology
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• 제14권4호
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• pp.543-546
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• 2005

Optimized conditions for protoplast preparation and regeneration from young hyphae of Monascus ruber were established. Heat shock treatment of spores gave rapid and synchronized germination. Spores collected from cultures grown for 7-8 days at $30^{\circ}C$ were germinated until over 70% germ tubes reached to 3-5 spore length. Enzymatic digestion of young hyphae was optimal with 50 mg/mL Glucanex in 0.1 M sodium citrate buffer containing 0.8 M mannitol as an osmotic stabilizer. Regeneration rate was around 10% when 0.8 M sorbitol was used as an osmotic stabilizer in regeneration medium. These conditions will be applied in genetic study of M. ruber that produces citrinin at high level and thus is good model strain for molecular genetic dissection of citrinin biosynthesis.

• Journal of Ginseng Research
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• 제16권1호
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• pp.13-17
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• 1992

The present experiments were performed to investigate the effects of the ginseng total saponin on the development of physical dependence on morphine via intracerebroventricular (i.c.v) route. Morphine (10 $\mu\textrm{g}$/${mu}ell$/hr) was continuously infused via osmotic minipumps into lateral cerebral ventricle of male Sprague Dawley rats for 7 days. Concurrent ginseng total saponin (100, 200 $\mu\textrm{g}$/10${mu}ell$/hr) was infused intraperitoneally (i.p) via osmotic pumps for 7 days. Treatment with ginseng total saponin (200$\mu\textrm{g}$/10${mu}ell$/hr) significantly diminished jumping, teeth chattering, hypothermia and weight loss precipitated by naloxone, compared with those animals received only morphine infusion. These results suggest that ginseng total saponin has central effect on the inhibition of physical dependence on morphine, as systemic ginseng total saponin inhibits the development of physical dependence in rats infused with morphine intracerebroventricularly.

• 한국미생물·생명공학회지
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• 제13권3호
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• pp.311-314
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• 1985

Novozym 234를 사용하여 Ganoderma lucidum의 원형질체 형성 및 재생 실험을 실시하였다. 원형질체 형성에는 Mannitol을 위시한 여러 가지 삼투압 조절제를 사용하여 $10^{6}$$m\ell$의 원형질체 형성이 가능하였다. 재생 실험에서는 Inositol과 Sucrose를 삼투압 조절제로 하여 0.26%까지의 재생율을 얻을 수 있었으나 MgSO$_4$ㆍ7$H_2O$는 재생용 배지의 삼투압 조절제로서는 부적합하였다. 재생 실험에서는 Bacteria등의 오염을 억제하기 위하여 Streptomycin sulfate를 첨가한 배지를 overlay 하였다.