• The Korean Journal of Physiology and Pharmacology
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• 제17권5호
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• pp.427-433
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• 2013

Receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclastogenesis is accompanied by intracellular $Ca^{2+}$ mobilization in a form of oscillations, which plays essential roles by activating sequentially $Ca^{2+}$/calmodulin-dependent protein kinase, calcineurin and NFATc1, necessary in the osteoclast differentiation. However, it is not known whether $Ca^{2+}$ mobilization which is evoked in RANKL-independent way induces to differentiate into osteoclasts. In present study, we investigated $Ca^{2+}$ mobilization induced by aluminum fluoride ($AlF_4^-$), a G-protein activator, with or without RANKL and the effects of $AlF_4^-$ on the osteoclastogenesis in primary cultured mouse bone marrow-derived macrophages (BMMs). We show here that $AlF_4^-$ induces intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) oscillations, which is dependent on extracellular $Ca^{2+}$ influx. Notably, co-stimulation of $AlF_4^-$ with RANKL resulted in enhanced NFATc1 expression and formation of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells. Additionally, we confirmed that mitogen-activated protein kinase (MAPK) is also activated by $AlF_4^-$. Taken together, these results demonstrate that G-protein would be a novel modulator responsible for $[Ca^{2+}]_i$ oscillations and MAPK activation which lead to enhancement of RANKL-mediated osteoclastogenesis.

• 물과 미래
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• 제29권5호
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• pp.139-150
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• 1996

본 논문에서는 수리학적 유역추적 모형인 선형 Muskingum-Cunge(M-C)법에 있어서 격자간격과 같은 수치적 인자의 변화가 단위폭사면에서의 유출수문곡선에 미치는 영향을 소개한다. 수치계산의 결과에 의하면, 유출특성은 수치적 또는 물리적으로 의미를 갖는 Courant 수 C 및 cell Reynolds 수 D의 값에 좌우되는데, C 값은 1에 접근할수록 D 값은 증가할수록 수치분산에 의한 진동은 발생하기 어렵다. C<1인 경우는 수치진동이 이동파의 전방에 발생한다. C>1인 경우는 파의 후방에 발생하는데, 이 때는 수치확산의 효과로 인해 수치진동은 작아지거나 사라진다. 특성구간길이 L의 값이 작은 사면(예, 급경사사면)의 경우, M-C법은 kinematic 법과 마찬가지로 파의 감쇠를 보이지 않는다. 한편 L의 값이 큰 사면(예, 완경사 사면)에서는 M-C법은 큰 거리격자간격 (Δx)에서도 큰 D(= L/ΔX)의 값을 갖게 되어 C에 거의 관계없이 diffusion wave를 잘 재현한다. 따라서 완경사 유역의 추적에 있어 M-C 법의 적용은 매우 유효하리라고 생각된다.

• 한국항공우주학회지
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• 제42권1호
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• pp.29-36
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• 2014

하이브리드 로켓 연소에서는 다양한 종류의 저주파수 연소 압력진동이 나타난다. 10Hz 대역의 저주파수 압력진동은 고체연료와 연소가스의 열 관성 차이 때문에 발생하지만 그외의 저주파수 진동은 고체로켓에서 관찰되는 헬름홀츠 및 $L^*$ 모드에 의해 발생하는 것으로 연소실 부피 변화와 밀접한 관련이 있다. 따라서 유동 특성이 고체로켓과 유사한 하이브리드 로켓 연소에서 연소실 부피 변화는 저주파수 특성에 영향을 미치는 중요한 인자이다. 본 연구에서는 연소실과 후연소실의 형상 변화에 따른 연소 압력의 저주파수 특성 변화를 관찰하였다. 특히 주 연소실과 후연소실의 부피 비가 특정한 값이 되면 연소 도중에 10~30Hz 연소 압력 진동의 진폭이 갑자기 증폭되는 연소불안정 현상이 나타났다. 산화제 유량 조절 및 연료 변경에 의한 O/F 비 변화는 연소 압력의 저주파수 증폭과 무관한 것으로 밝혀졌다. 후연소실로 연소가스가 팽창할 때 발생하는 와류 흘림 현상이 저주파수 불안정 현상과 직접적인 관련 있는 것으로 판단되며 이에 관한 연구가 더 필요하다.

• 천문학회보
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• 제39권2호
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• pp.98-98
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• 2014

Due to the simple vertical structure of magnetic field, pores can be exploited to study the transport of mechanical energy by waves along the magnetic field to the chromosphere and corona. For a better understanding of physics of pores, we have investigated chromospheric traveling features running across two merged pores from their centers at the speed about 55 km s-1, in the active region AR 11828. The pores were observed on 2013 August 24 by using high time, spatial, and spectral resolution data from the Fast Imaging Solar Spectrograph (FISS) of the 1.6 meter New Solar Telescope (NST). We infer a LOS velocity by applying the bisector method to the Ca II $8542{\AA}$ band and $H{\alpha}$ band, and investigate intensity and the line-of-sight velocity changes at different wavelengths and different positions at the pores. We find that they have 3 minutes oscillations, and the intensity oscillation from the line center is preceded by that from the core ($-0.3{\AA}$) of the bands. There is no phase difference between the intensity and the LOS velocity oscillations at a given wavelength. The amplitude of LOS velocity from near the core spectra is greater than that from the far core spectra. These results support the interpretation of the observed wave as a slow magnetoacoustic wave propagating along the magnetic field lines in the pores. The apparent horizontal motion and a sudden decrease of its speed beyond the pores can be explained by the projection effect caused by inclination of the magnetic field with a canopy.

• International Journal of Fluid Machinery and Systems
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• 제8권2호
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• pp.102-112
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• 2015

In the Nordic grid, a trend observed the recent years is the increase in grid frequency variations, which means the frequency is outside the normal range (49.9-50.1 Hz) more often. Variations in the grid frequency leads to changes in the speed of rotation of all the turbines connected to the grid, since the speed of rotation is closely related to the grid frequency for synchronous generators. When the speed of rotation changes, this implies that the net torque acting on the rotating masses are changed, and the material of the turbine runners must withstand these changes in torque. Frequency variations thus leads to torque oscillations in the turbine, which become dynamical loads that the runner must be able to withstand. Several new Francis runners have recently experienced cracks in the runner blades due to fatigue, obviously due to the runner design not taking into account the actual loads on the runner. In this paper, the torque oscillations and dynamic loads due to the variations in grid frequency are simulated in a 1D MATLAB program, and measured grid frequency is used as input to the simulation program. The maximum increase and decrease in the grid frequency over a 440 seconds interval have been investigated, in addition to an extreme event where the frequency decreased far below the normal range within a few seconds. The dynamic loading originating from grid frequency variations is qualitatively found by a constructed variable $T_{stress}$, and for the simulations presented here the variations in $T_{stress}$ are found to be around 3 % of the mean value, which is a relatively small dynamic load. The important thing to remember is that these dynamic loads come in addition to all other dynamic loads, like rotor-stator interaction and draft tube surges, and should be included in the design process, if not found to be negligible.

• International Journal of Oral Biology
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• 제45권2호
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• pp.58-63
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• 2020

The salivary glands secrete saliva, which plays a role in the maintenance of a healthy oral environment. Under physiological conditions, saliva secretion within the acinar cells of the gland is regulated by stimulation of specific calcium (Ca²⁺) signaling mechanisms such as increases in the intracellular Ca²⁺ concentration ([Ca²⁺]_i) via storeoperated Ca²⁺ entry, which involves components such as Orai1, transient receptor potential (TRP) canonical 1, stromal interaction molecules, and inositol 1,4,5-triphosphate (IP₃) receptors (IP₃Rs). Homer proteins are scaffold proteins that bind to G protein-coupled receptors, IP₃Rs, ryanodine receptors, and TRP channels. However, their exact role in Ca²⁺ signaling in the salivary glands remains unknown. In the present study, we investigated the role of Homer2 in Ca²⁺ signaling and saliva secretion in parotid gland acinar cells under physiological conditions. Deletion of Homer2 (Homer2^-/-) markedly decreased the amplitude of [Ca²⁺]_i oscillations via the stimulation of carbachol, which is physiologically concentrated in parotid acinar cells, whereas the frequency of [Ca²⁺]_i oscillations showed no difference between wild-type and Homer2^-/- mice. Homer2^-/- mice also showed a significant decrease in amylase release by carbachol in the parotid gland in a dose-dependent manner. These results suggest that Homer2 plays a critical role in maintaining [Ca²⁺]_i concentration and secretion of saliva in mouse parotid gland acinar cells.

• Ocean and Polar Research
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• 제31권2호
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• pp.177-187
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• 2009

This study examined the multi-scale variabilities of sea surface temperature (SST) and salinity in the Japan/East Sea (JES) based on statistical analyses of observational data, with a focus on the northwestern part of the sea. The regionality of JES SST variability was estimated for different frequency ranges on semimonthly (11-17 days), monthly to seasonal (30-90 days), quasi-semiannual (157-220 days), and quasi-biennial (1.5-3 years) time scales using cluster analyses of daily gridded SST data for 1996 to 2007 from the Japan Meteorological Agency (JMA). Several significant peaks and regional cores were found in each frequency range of the SST anomaly (SSTA) oscillations. Quasi-semiannual SSTA oscillations with high amplitude were found in the south-southwestern part of the Japan Basin ($41-43^{\circ}N$) and were amplified in the area adjacent to Peter the Great Bay. Oscillations with periods of 79 and 55 days also prevailed over the southwest Japan Basin between the Yamato Rise and the continental slope. A similar method was applied to classify SST and the annual cycle of surface salinity using Generalized Digital Environmental Model (GDEM) gridded data. The Tatarskii Strait and adjacent area showed the most specific annual cycles and variability in salinity on interannual to interdecadal time scales. The most significant inverse relationship between surface salinity in the Tatarskii Strait and southern JES areas was found on the interdecadal time scale. Linkages of sea water salinity in the Tatarskii Strait with Amur River discharge and wind velocity over Amurskii Liman were also revealed.

• 대한기계학회논문집B
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• 제34권7호
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• pp.729-738
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• 2010

질소로 희석된 부탄 층류 부상 화염에서 발생할 수 있는 화염진동 메커니즘을 살펴보기 위하여 실험적 연구를 수행하였다. 화염 진동은 층류 자유제트 부상 화염에서 5가지 영역으로 구분되었다: 화염 안정화 영역 (I), 열손실에 의한 진동 (II), 열손실에 의한 진동과 부력에 의한 진동이 혼재된 영역 (III),열손실에 의한 진동과 화염날림 직전의 진동이 혼재된 영역 (IV), 그리고 열손실에 의한 진동, 부력에 의한 진동 및 화염날림 직전의 진동이 모두 혼재된 영역(V). 각각의 화염진동의 특성을 규명하기 위해 화염의 시간에 따른 부상 높이 변화에 대한 FFT분석을 수행하였고 각 영역에 관련된 무차원 변수와 스트라훌 수의 조합으로 특성화 작업을 수행하였다.

• Clinical and Experimental Reproductive Medicine
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• 제44권1호
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• pp.15-21
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• 2017

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

• 한국전산구조공학회논문집
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• 제26권6호
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• pp.463-470
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• 2013

본 연구에서는 풍진동에 대한 현수교의 거동을 예측하기 위하여 바닥판의 비틀림강성을 고려하여 Mckenna and Tuama 모델(2001)을 개선한 2D 간단해석 방법을 제안하였다. 기존의 모델은 풍속이 증가할수록, 진동수가 낮아질수록 비정상적인 값을 나타내고, 비틀림모드의 공진현상을 묘사할 수 없었다. 이에 본 연구에서는 비틀림강성을 고려하여 풍속에 따른, 진동수에 따른 비틀림진동을 분석하였다. 해석결과 진동 초기의 수직모드는 점차 비틀림모드로 전이되며 수직모드는 안정적으로 진동하는 것을 확인하였다. 또한 비틀림강성 효과를 고려하여 해석을 수행한 결과 수직모드는 시간이 경과함에 따라 안정화되는 모습을 보이나 비틀림 진폭은 일정시간(약 200초) 이후 나타나기 시작하여 비틀림각을 지속적으로 유지하였으며 맥놀이 주기는 풍속이 증가하면서 점차 감소하였다. 비틀림 강성에 따라 서로 다른 풍하중의 풍속과 진동수에 비틀림모드의 공진현상을 나타내므로 실제 설계에는 반드시 이러한 영향이 고려되어야 할 것이다.