• Animal Systematics, Evolution and Diversity
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• 제8권2호
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• pp.177-182
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• 1992

1986년 4월부터 1991년 2월까지 한국의 서남해로 유입하는 하천과 인접 도서지방에 서식하는 송사리 Oryzias latipes의 표본을 조사한 바, 조사수역의 모든 표본은 척추골 수가 28-31개이고, 가슴지느러미 기조수는 9개이며, 2n 염색체 수가 46개 이었다. 이러한 특징은 동해로 유입하는 하천과 남해의 인접 도서지방에 분포하는 O. latipes latipes와는 구별되고, 중국산 O.latipes sinensis와 일치되어 한국 미기록 아종으로 보고하고 이 아종의 국명은 대륙송사리로 명명한다.

• 한국환경농학회지
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• 제6권2호
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• pp.66-72
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• 1987

잉어(C. carpio), 송사리(O. latipes), 일본산 송사리(O. latipes)등 3종의 담수산어 류에 대한 30개 농약의 급성독성$(48hr-LC_{50})$을 평가하여 각 어종간 농약에 대한 감수성의 상관관계를 비교하였다. 어종간 농약감수성의 상관관계는 잉어와 송사리는 r=0.80, 잉어와 일본산 송사리는 r=0.89로 비교적 높은 편이나, 송사리와 일본산 송사리의 상관관계는 r=0.93으로 매우 높았다. 따라서 일본산 송사리의 실험결과로 잉어 및 송사리의 농약 감수성을 예측할 수 있을 것으로 생각되었다. 그리고 우리나라의 잉어에 대한 어독성실험 지침은 실험수조내의 DO가 2mg/1 정도까지 떨어지므로 실험지침에 대한 검토가 있어야 할 것이다.

• 한국어류학회지
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• 제24권4호
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• pp.247-252
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• 2012

한국산 송사리속 2종에 대한 섭이기관의 구조적 차이를 알아보기 위하여 악골 및 이빨 구조를 조사하였다. 악골에서 송사리 Oryzias latipes는 전상악골 중앙부의 등 쪽 가장자리에 움푹 들어가는 홈이 존재하였으나 대륙송사리 O. sinensis는 일직선이었다. 후반부의 위치에서 송사리는 중앙부와 일직선상에 존재하였으나 대륙송사리는 배 쪽에 존재하였다. 또한 송사리는 하악골의 구상돌기 아래로 이어지는 치골 뒷가장자리에 움푹 들어가는 홈이 존재한 반면에 대륙 송사리에서는 뒷가장자리가 일직선 모양을 하였다. 송사리는 성적이형으로서 수컷에서만 나타나는 큰 이빨이 하악골의 측면 방향으로 발달하였고, 대륙송사리는 등 쪽 방향으로 발달하였다. 한편 송사리의 이빨형태는 원뿔모양과 화살촉모양 2 types이 나타난 반면에 대륙송사리에서는 원뿔 모양 1 type만 나타나는 특징을 보였다. 이상과 같이 한국에 분포하는 2종의 송사리는 섭이기관에서 뚜렷한 종간의 특징을 잘 보여 주었다.

• Journal of Ecology and Environment
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• 제36권3호
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• pp.159-166
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• 2013

Oryzias latipes and Oryzias sinensis are indigenous species found in Japan, China, and other East Asian countries, including Korea. Based on morphological differences, the species have been classified distinctly. However, the range of morphological characters such as the number of gill rakers, vertebrae, and spots on the lateral body overlaps and is too vague for clear identification, so their classification based on their morphological characteristics remains uncertain. In this study, the mitochondrial cytochrome oxidase subunit I (COI) gene, which is used for DNA barcoding, was applied to clarify interspecific variation of O. latipes and O. sinensis. Intraspecific genetic diversity was calculated to identify correlations with geographic distributions. We studied two species collected from 55 locations in Korea. All individuals carried a 679-base pair gene without deletion or insertion. Between species, 525 base pairs of the gene were shared. The Kimura two parameter (K2P) distance of O. latipes and O. sinensis was 0.41% and 1.39%, respectively. Mean divergence within genera was 23.5%. Therefore, the species were clearly different. The distance between O. latipes and O. sinensis was 14.0%, which is the closest within genera. Interestingly O. latipes from the Japanese and Korean group represented 16.5% distant. These results were derived from geohistorical and anthropogenic environmental factors. The O. latipes haplotypes were joined in only one group, but O. sinensis was divided into two groups, one is found in the Han River and upper Geum River watershed; the other is found in the remaining South Korean watersheds. Further studies will address the causes for geographic speciation of O. sinensis haplotypes.

• Environmental Engineering Research
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• 제22권4호
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• pp.426-431
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• 2017

Some phototoxicity of titanium dioxide nanoparticles ($TiO_2$ NPs) has been reported in recent years in studies with fish embryos or larvae. However, it is necessary to focus on the potential effects of embryonic exposure due to irreversible abnormalities and mortalities observed in sac-fry, and to expand various fish embryos to generate multiple test species. The aim of this study was to evaluate the effects of $TiO_2$ NPs under different irradiation conditions in exposed Oryzias latipes (O. latipes) at the embryonic and sac-fry stages. The effects of different irradiation conditions were observed using ultra-violet (UV) and visible light, and the corresponding effects were monitored by determining cumulative mortality and abnormality. O. latipes were exposed for 8 d to 0, 1, 5, 10, or 50 mg/L $TiO_2$ NPs under UV ($4,818.86mW/m^2$ at the bottom of clear vials) or visible light, after which the embryos were transferred to NP-free embryo-rearing solution until 16 days post fertilization (dpf). Abnormalities of embryos and sac-fry increased at high $TiO_2$ NP concentrations under UV irradiation, compared to control samples treated with visible light or UV irradiation alone. This work provides information regarding the phototoxicity of $TiO_2$ NPs using O. latipes at the embryonic and sac-fry stages.

• Environmental Analysis Health and Toxicology
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• 제20권2호
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• pp.187-194
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• 2005

Octachlorostyrene (OCS) is a primarily concerning chemical in many countries because of its persistent and bioaccumulative properties in the environment. OCS is not commercially manufactured or used but it may be produced during incineration or chemical synthetic processes involving chlorinated compounds. There are several reports that OCS was found in the waters, sediments, fish, mussels, and also in human tissues. However, systematic studies on the OCS toxicities are scarce in literature. In this study, we tried to get the gene expression data using medaka DNA chip to identify biomarkers of OCS exposure. Medaka (Oryzias latipes.) was exposed to OCS 1 ppm for 2 days and 10 days, respectively. Total RNA was extracted and purified by guanidine thiocyanate method and the Cy3- and Cy5-labelled cDNAs produced by reverse trancription of the RNA were hybridized to medaka microarray. As results, eighty five genes were found to be down-or up regulated by OCS. Some of the genes were listed and confirmed by real-time PCR.

• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.173-178
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• 2014

In order to monitor the developmental features of embryos, larvae, and juveniles of Oryzias latipes (Temminck and Schlegel), Oryzias latipes was caught in river of Shinduck-dong, Yeosu-si, Jeollanam-do, on May 2011, and experiments were carried out in Ichthyology laboratory at Chonnam National University. The blastodisc step was the first level for natural spawning. The optic vesicle, Kupffer's vesicle, myotome began to appear 75 hours 57 minutes later. After blastodisc development, the pectoral fins were made at 143 hours 37 minutes and the tail was separated started at the same time. Hatching was observed at 167 hours 27 minutes after blastodisc. The total length of the hatched larvae was 4.95~5.10 mm (mean, 5.01 mm), the mouth and anus were opened. Larvae used yolk completely after 3 days after hatching. The total length larvae was 5.45~5.56 mm (mean, 5.52 mm) after 8 days after hatching, and appeared the stems for tail. The stems pectoral, anal fin were showed after 14 days and the stems dorsal, ventral fin were appeared after 19 days. For 35 days after hatching, the total length of larvae 13.95~15.30 mm (mean, 14.64 mm), and at this time, fins and body were transferred like the adult Oryzias latipes.

• Environmental Analysis Health and Toxicology
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• 제25권2호
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• pp.145-151
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• 2010

Perfluorinated chemicals (PFCs) is a kinds of persistent organic pollutants, and have the potential toxicity of which is causing great concern. In this study, we employed Oryzias latipes embryos to investigate the developmental toxicity of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA)s compound using flowthrow system for 14 day. O. latipes embryos were exposed to solvent control, 20, 40 and 80 mg/L of PFOS and 62.5, 130, 260 mg/L of PFOA respectively. After exposure, hatchability, mortality, total length and heart beats were examined. Hatching rates were reduced approximately 27% in the 80 mg/L PFOS-treated group and 17% in the 62.5, 130 mg/L PFOA-treated groups. Heart beats in the PFOS-treated groups were reduced at 7 day but, PFOA-treated groups were increased heart beats. 80 mg/L PFOS treated group showed significant reduction in growth (total length) level to 90% of control. But PFOA did not showed significant effect on growth. In the 14 days $LC_{50}$ of PFOS and PFOA was 22.74 mg/L and 173 mg/L, respectively. The overall results indicated that the early stage of O. latipes might be a reliable model for the testing of developmental toxicity to perfluorinated chemicals.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.155.1-155
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• 1997

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2005년도 Proceedings of KSEH.Minamata Forum
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• pp.54-57
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• 2005

Endocrine disrupting effects of several pharmaceutical products were evaluated with fish. The test pharmaceuticals(caffeine, ketoconazole, acetaminophen and diltiazem) have been often detected in aquatic environment of Korea(from on going study of this research team). We analyzed vitellogenin induction by qualitative (Western blot) and quantitative (ELISA) assay. $17{\beta}$ -estradiol was used as a positive control. Some pharmaceuticals could give effects to male Oryzias latipes. They could induced vitellogenin under exposure of chemicals at male Oryzias latipes.