• Journal of Plant Biotechnology
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• v.1 no.2
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• pp.91-95
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• 1999

Genetic engineering of stone fruit species like apricot, plum, peach and cherry are hampered by the inefficient and low-level regeneration processes in tissue culture. The first transgenic stone fruit species have emerged from transformed hypocotyls. These great achievements were applauded by the scientific community contrary the fact that hypocotyl derived transgenic plants have no real brooding value. Tissue culture of different organs of valuable cultivars are recorded with an extremely low-level of regeneration in the literature. To improve the tissue culture basis of stone fruit plants an extensive tissue culture programme were launched and dozens of different media were compared including a series of hormone concentration in the tissue culture systems. Our continuous efforts were crowned by a very efficient method for achieving up to 30-40% regenerable petioles. Usually on a single petiole several well-separated meristems were induced. After 3-4 weeks of cultivation shoots were developed. The basic media $K_2$ were supplemented with 10g/L saccharose, 10g/L glucose and 10g/L maltose. The following plant hormones were used BAP 1mg/L, TDZ 1mg/L, 2-iP 1mg/L and IAA 0,1 mg/L concentrations. The Petri dishes were kept for 3 weeks in dark at a temperature 22$^{\circ}C$ for 8 hours and 22-24$^{\circ}C$ for 16 hours. The Petri dishes were sealed with Parafilm. The regeneration of the petioles were genotype independent and we were able to regenerate different plum cultivars with almost the same efficiency.

• Korean Journal of Plant Resources
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• v.30 no.2
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• pp.191-213
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• 2017

This study was conducted to elucidate the distribution of vascular plants and their value of Mt. Samyeong. The vascular plants that were collected 10 times from October 2013 to July 2015 consisted of a total of 538 taxa; 92 families, 308genera, 466 species, 3 subspecies, 62 varieties and 7 forms. The observed rare plant species as designated by Korea Forest Service were 24 taxa. Endemic species were 25 taxa. 28 taxa were recorded as naturalized plants and their naturalized rate and urban index were noted as 5.3% and 8.7%. Among the surveyed 538 taxa, edible, medicinal, pasturing, ornamental, timber, dye, fiber and industrial plants included 220 taxa, 195 taxa, 167 taxa, 65 taxa, 25 taxa, 8 taxa, 6 taxa and 3taxa, respectively.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.557-563
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• 2016

To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the temperature for seed germination increased, up to $30^{\circ}C$, while the mean germination time (MGT) and the mean number of days to 30% germination ($T_{30}$) decreased when seeds were germinated at $25-30^{\circ}C$. The optimum germination temperature of S. fritschiana seeds is approximately $30^{\circ}C$ considering FGP, MGT, and $T_{30}$ together. FGP increased with the duration of cold stratification up to a period of 6 weeks, but declined after 8 weeks of cold stratification, as prolonged cold stratification can induce dormancy with a resultant decline in germination. Pretreatment with 6-8 weeks of cold stratification or soaking seeds in distilled water or $500mg{\cdot}L^{-1}$ $GA_3$ for 24 h accelerated and increased the germination of S. fritschiana seeds, regardless of temperature. However, further study might be required to evaluate the effect of $GA_3$ concentrations lower than $500mg{\cdot}L^{-1}$ on the promotion of germination in S. fritschiana seeds.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.157-163
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• 2010

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.564-583
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• 2011

The flora of Gitdae-bong area were investigated from March, 2009 to September, 2010. The vascular plants were summarized as 435 taxa; 90 families, 272 genera, 380 species, 3 subspecies, 45 varieties, and 7 forma. Among the investigated 435 taxa, twelve Korean endemic, eight rare plants and 68 specially designated plants by Ministry of Environment were also included. The naturalized plants were 15 taxa and percent of naturalized plant species was 3.4%. Usefulness of plants of 435 taxa listed consists of 203 taxa (46.7%) of edible plants, 141 taxa (32.4%) of medicinal plants, 64 taxa (14.7%) of pasture plants, 44 taxa (10.1%) of ornamental plants, 18 taxa (4.1%) of timber plants, 15 taxa (3.4%) of fiber plants and 4 taxa (0.9%) of industrial plants.

• Korean Journal of Plant Resources
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• v.29 no.2
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• pp.171-188
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• 2016

This study was carried out to investigate the flora of Mt. Baegam (1,099 m) from March, 2014 to July, 2015. The vascular plants were summarized as 506 taxa, including 90 families, 293 genera, 435 species, 5 subspecies, 58 varieties and 8 forms. Among the investigated 506 taxa, 14 Korean endemic, 16 rare plants and 80 specially designated plants (V degree: 3 taxa, IV degree: 7 taxa, III degree: 22 taxa, II degree: 21 taxa, I degree: 27 taxa) by the Ministry of Environment were also included. The naturalized plants were 36 taxa including 3 ecosystem disturbance wild plant. The urbanization index and percent of naturalized plants species were estimated as 11.2% and 7.1%, respectively. Useful plants of 506 taxa listed consists of 207 taxa (40.9%) of edible plants, 200 taxa (39.5%) of pasture plants, 146 taxa (28.9%) of medicinal plants, 58 taxa (11.5%) of ornamental plants and 15 taxa (3.0%) of timber plants, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.114-114
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• 2018

Among the various volatile organic compounds (VOCs) emitted by the plant, floral scent plays a key role in attracting pollinators for reproduction and mediates ecological interactions. Floral scent is an important trait and industry drives the competition for flowers with novel scents. Chrysanthemum is one of the well-known ornamental plants and is a popular cut flower across the world. Floral scent and the genes responsible for the floral scent emission are poorly studied in chrysanthemum. In the present study, floral scent and the expression pattern of floral scent genes were analyzed in two chrysanthemum cultivars 'Golden Egg' and 'Gaya Glory'. Initially, intensity of the floral scent in five developing stages of flower including 'budding (B), bud developing (BD), initial blooming (IB), almost open (AO) and open flower (OF)' was analyzed using electronic nose (E-nose) with six metal oxide sensors. Based on the distance analysis, different stages of flower showed different relative intensity of scent according to the sensory evaluation. Although the scent pattern differed by stage, scent intensity was strongest in the OF stage in the completely opened flower in both the cultivars. Further, expression pattern of six genes in the floral scent pathway including FDS, IDI, ISPH, TPS2, TPS5 and TPS6 was observed in all the five stages of the flower in both the cultivars. The expression pattern of all the six genes differed by stage and the terpene synthase genes TPS2, TPS5 and TPS6 showed good expression levels in the $5^{th}$ flower stage compared to other stages. This study provides a preliminary data for understanding the regulation of floral scent in chrysanthemum.

• Journal of Environmental Science International
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• v.20 no.4
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• pp.527-540
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• 2011

The investigation was made about distribution and ecological characteristics of Rhododendron mucronulatum var. mucronulatum, R. dauricum and R. schlippenbachii on Mt. Changbai in China. The Ericaceae plants are known to be highly ornamental and easy to cultivate. The results of investigations are like followings. The plant species diversity was the highest in habitats of R. mucronulatum var. mucronulatum, followed by those of R. schlippenbachii and R. dauricum. R. mucronulatum var. mucronulatum community was found in the northwest exposure areas of 300m in altitude. Corylus heterophylla, Carex humilis var. nana, Atractylodes japonica species also grew in the R. mucronulatum var. mucronulatum community. R. dauricum communities were widely distributed in the west exposure areas of 1226-1860m above sea level. R. schlippenbachii populations were found in areas of under 300m above sea level, which has dry soil. But humidity of air was relatively high in the place. Artemisia japonica, Lespedeza bicolor and Dactylis glomerata were found around the habitats of R. schlippenbachii. Phalanx type of plants were dominant near the habitats of Ericaceae plants as 61%. Further, there appeared higher plant life totalling to 72 taxa comprising 54 species, 16 varieties and 2 forma with 31 families and 59 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 30.0%.

• Journal of Environmental Science International
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• v.23 no.7
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• pp.1299-1305
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• 2014

For evaluating the effect of various artificial planting soil properties on the Euonymus fortunei 'Emelad'n Gold' growth, a container green wall system experiment was conducted in a wall of greenhouse at Konkuk University, Glocal campus. The experimental artificial planting grounds were prepared with different organic soil conditioner ratios (Control, $A_4O_1$, $A_2O_1$ and $A_1O_1$) and with drought tolerance and an ornamental value Euonymus fortunei 'Emelad'n Gold' was planted. The soil and plant characteristics were investigated from April to Jun 2010. The volumetric soil moisture contents were significantly increasing order as the amount of organic soil conditioner level increased in order to $A_1O_1$ > $A_2O_1$ > $A_4O_1$ > Control. At 4 treatment, soil chemical properties were inversely related to organic soil container ratios increase. The differences of root collar caliper, number of branch, and survival rate between the organic soil conditioner ratio were not significantly affected by organic soil conditioner. But, plant height, internode length, leaf length and leaf width were significantly shorter on plants planted $A_1O_1$ than plants planted other treatments. Therefore, Euonymus fortunei 'Emelad'n Gold' had good growth response regardless of organic soil conditioner ratio and the plant is expected to be a highly valuable shrub for the green wall system if it should be considered in integration with stormwater retention or as a soil conditioner for increasing soil water contents in artificial planting soil.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.281-291
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• 2007

This study was carried out to clarify the distribution of vascular plants and their usefulness in Geommasan of Gyeongbuk (1,017m, $36^{\circ}\;42^{'}\;30.0^{"}{\sim}36^{\circ}\;45^{'}\; 30.1^{"}N,\;129^{\circ}\;12^{'}\;30.0^{"}{\sim}129^{\circ}\;18^{'}\; 00.0^{"}E$). The vascular plants collected 11 times (from April 2004 to October 2006) were consisted of total 513 taxa; 91 families, 318 genera, 424 species, 3 subspecies, 73 varieties and 13 forms. Among them, edible, medicinal, industrial, ornamental and unknown of usefulness plants were 243 taxa, 307 taxa, 122 taxa, 85 taxa, 77 taxa, respectively. Korean endemic plants of this area were 11 taxa, and Law-protected plants by Ministry of Environment were 4 taxa, The naturalized plants were 21 taxa, therefore naturalized ratio was 4.1%, and Urban index 7.5%.