• The Journal of Internal Korean Medicine
• /
• v.21 no.2
• /
• pp.259-266
• /
• 2000

Objectives : The effects of aqueous extracts of Cortex ulmi pumilae (a traditional medicine for cancer treatment in oriental medicine) on the induction of apoptotic cell death were investigated in human liver origm hepatoma cell lines, HepG2. Methods : The death of HepG2 cells was markedly induced by the addition of extracts of Cortex ulmi pumilae in a dose-dependent manner. The apoptotic characteristic ladder pattern of DNA strand break was not observed in cell death of HepG2. In addition, it was not shown nucleus chromatin condensation and fragmentation under hoechst staining. However, by the using annexin V staining assay, externalizations of phosphatidylserine in HepG2 cell which were treated with Cortex ulmi pumilae extracts were detected in the early time (at 9 hr after extract treatment). Furthermore, LDH release was not detected in this early stage. Therefore, Cortex ulmi pumilae extracts-induced cell death of HepG2 cells is mediated by apoptotic death signal processes. Result : The activity of caspase 3-like proteases remained in a basal level in HepG2 cells which treated with the extract of Cordyceps sinensis. However, it was markedly increased in HepG2 cells which treated with two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K) which were differently extracted (respectively, 2.3 and 3.3 fold). On a while, the phosphotransferase activities of JNK1 was markedly induced in HepG2 cells which were treated with two extracts of Cortex ulmi pumilae. On the contrary, the activation of transcriptional activator, activating protein1(AP-1) and NF-kB were severely decreased by these two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K). In addition, antioxidants (GSH and NAC) and intracellular $Ca2^+$ level regulator (Bapta/AM and Thapsigargin) did not affect Cortex ulmi pumilae extracts-induced apoptotic death of HepG2 cells. Conclusions : In conclusion, our results suggest that two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K) induces the apoptotic death of human liver origin hepatoma HepG2 cells via activation of caspase 3-like proteases as well as JNK1, and inhibition of transcriptional activators, AP-1 and $NK-{\kappa}B$.

• Journal of Preventive Medicine and Public Health
• /
• v.15 no.1
• /
• pp.57-73
• /
• 1982

Questionaires on symptoms of respiratory disease have been used in Korea to elicit the probable health effects of air pollution in epidemiologic studies: The objectives of such studies often include comparing prevalence of symptoms of respiratory system between different population groups or between the same population groups, at different times. Unfortunately, little attention has, been paid to standardization of those questionaires, whether those are Korean. versions or not. Furthermore, no attempt to develop Korean ,questionaire on respiratory symptoms and relevant information has been made. Followed by 'a comparative study on responses to Korean version questionaires(English origin) of CMI, MRC, and ATS-DLD-78' two types of questionaires on respiratory symptoms and relevant information for Korean adult, which are short form (SUN-81-AS) and long forms (optional questions are added to the short one, SUN-81-AL), have been designed suitable to Korean background by authors (see Annex). The self-administered and closed-question questionaire were tested their validity and reliability by administration to l80 normal adults (medical and nursing students) and 60 clinical patients of Seoul National University Hospital, with spirometric exam. The results obtained and conclusions drawn are as follow: 1. It took less than 10 minutes to complete the questionaire SNU-81-AS and SNU-81-AL. 2. The test-retest reliability of each questions in AS and AL ,were observed as 92.7% and 92.1%, respectively. And all of the level of agreement are statistically significant with kappa statistic. 3. In addition to higher prevalence rate of symptoms in patients group compared, with, normal. group, the correlations between FEV 1.0/FVC predictive value(%) and number of symptoms were statistically significant inpatients group (See Fig. 1 and, Table 7). 4. The answer rate to optional questions in AL form among those who are not to do was about 10%, while the no-answer rate among who are to do was about 15% in Normal (medical and nursing students) group. 5. From the viewpoints of validity and reliability, the new Korean questionaire (SNU-81-AS and AL) developed by authors are to be recommendable to use in epidemiologic studies on respiratory illness in Korea. The self-administration, however, of optional questions in AL form may not assure the quality of data gathered.

• Journal of the Korean Institute of Gas
• /
• v.15 no.5
• /
• pp.25-30
• /
• 2011

Biogas obtained from the biodegradable organic wastes in an anaerobic digester consists of $CH_4$ and inert gases such as $CO_2$ and $N_2$. Since the composition of biogas varies by anaerobic digester conditions and the origin of wastes, it is necessary to respond to these variations so as to make stable combustion and accomplish high efficiency when it is used as a fuel for power generating SI engines. In this study, efforts have been made to investigate the effect of changes in the calorific values of biogas on the engine performance and exhaust characteristics. The biogas was simulated by supplying of $CH_4$ with $N_2$ dilution of various ratios, and ECM was developed to achieve accurate control of ignition and combustion. The results show that as the $CH_4$ concentration of the biogas decreases, the optimal spark timing is advanced due to the elevated thermal capacity and lowered $O_2$ concentration of the in-cylinder charge. Furthermore, since combustion temperature was reduced by increased inert gas, $NO_x$ emissions decreased, whereas THC emissions increased.

• Ocean and Polar Research
• /
• v.30 no.4
• /
• pp.519-536
• /
• 2008

The relatively tranquil area within the Gogunsan Archipelago was for the first time investigated preliminarily with respect to modern sedimentological processes in association with the emplacement of the Saemangeum Dyke. Basic sedimentological observations, bathymetry and surface sediments were performed twice during 2006-2008 to compare the results and elaborate changes during that period of time. In addition, sediment dynamical observations were carried out with latest measuring equipment along two transects crossing the entrances of the archipelago, including 12-hour onboard measurements of current, suspended sediments, temperature, and salinity. This dataset was used to reveal hydrodynamic characteristics for spring season April-May and to estimate the direction and relative magnitude of the net flux of suspended sediments. There occurred three depositional areas (A to C) within the archipelago, where sediment texture was also changed. In area A, around Yami Island and the dyke, and area B, in the center of the archipelago, surface sediments became coarsened over the two-year period; sand content increased 5% at the expense of silt content in the former, whereas silt content increased 3% at the expense of clay content in the latter. By comparison, area C in the western entrance of the archipelago shows a textural trend of fining with more silt and clay (combined increase of 5%) at the expense of sand content. The accumulation of sediments in areas A and B is attributable to the sand and silt resuspended from the seabed sediments off sector 4 of the dyke during the winter. The origin of the fine materials depositing on area C is uncertain at present, although suspended sediments moving offshore around the archipelago may be one of the most likely candidates for the source. The temperature of seawater increased rapidly from $9-10^{\circ}C$ in April to $14-16^{\circ}C$ in May, whereas salinity remained more or less constant at 31-32%o during the two months. Both of these parameters showed little variations with depth through a tidal cycle, suggesting good mixing of seawater without any help of significant waves. The consistency of salinity during a tidal cycle also indicates no insignificant effects of freshwater from the rivers Mangyung and Donjin emitting through the opening gap near Sinsi Island. The suspended sediment concentrations were higher at the entrance between Sunyu and Sinsi islands than at the entrance between Hoenggyong and Sinsi islands, ranging from 20 and 30 mg/l and from 5 and 15 mg/l, respectively at the sea surface. Although tidal currents were variable across a transect between Sunyu and Sinsi islands, the currents across the entrance between Hoenggyong and Sinsi islands flowed consistently in the same direction all over the transect during a tidal cycle. The estimation of net flux of suspended sediments indicates that suspended sediments are transferred to the Gogunsan Archipelago mainly through a relatively deep trough adjacent to Sinsi Island toward the shallow area around Yami Island and the dyke.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.8
• /
• pp.1078-1084
• /
• 2009

The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis of variation in the Jindo coat color, we sequenced the protein-coding regions of the melanocortin 1 receptor gene (MC1R). The MC1R coding sequence was determined from 154 dogs in five breeds (Jindo, Labrador Retriever, English Springer Spaniel, Belgian Malinois, and German Shepherd). To confirm the genetic structure of sampled populations, we tested for Hardy-Weinberg equilibrium (HWE) and computed $F_{st}$ The sample populations did not significantly deviate from HWE. $F_{st}$ was 0.02 between white and fawn Jindo dogs; this was lower than $F_{st}$ between breeds. Six single nucleotide polymorphisms (SNPs) were detected in the MC1R coding region. Among the six SNPs, five were non-synonymous (S90G, T105A, Q159P, M264V, and R306ter) and one was synonymous SNP (Y298Y). From the SNPs, we predicted four haplotypes (H1, H2, H3, and H4) for Jindo MC1R. Jindo dogs had different haplotypes corresponding to different coat colors. H1 was frequently observed in white Jindo dogs with an odds ratio of 5.03 (95% CI: 2.27-11.18, p<0.0001), whereas H2 and H4 were observed only in fawn Jindo dogs. Our findings indicate that SNP haplotype can influence coat color. Knowledge of MC1R haplotypes can help discriminate white and fawn coats in Jindo dogs. We hope this report will trigger more research into the genetics of this traditional Korean dog and will be a reference for dogs of Asian origin. Also, our results will provide a useful genetic marker for Jindo dog breeders who have selected for specific colors.

• The Korean Journal of Pesticide Science
• /
• v.2 no.2
• /
• pp.75-82
• /
• 1998

This study was conducted to find out the biochemical or metabolic resistance mechanism of brown planthopper (BPH) to carbofuran. Differences between resistant ($LD_{50};\;20.3{\mu}g/g$) and susceptible strains($LD_{50};\;0.3{\mu}g/g$) were shown. The amounts of carbofuran metabolite, benzofuranol, and the origin, not developed by Thin Layer Chromatography, were much more in the susceptible strain. But the mother compound, carbofuran, was much more in the resistant strain. The tendencies of metabolism one and three hours after treatment were similar in both strains except for the amounts of metabolites described above. From the study, it is supposed that hydrolytic enzyme, esterase, changes its role from cleaving the esteric bond of carbofuran to making conjugates with carbofuran. This seems to be the main resistance mechanism of BPH to carbofuran. Oxidase and transferase may play little or no role in resistance mechanism. Oxidative and transferring enzymes gave no effects on the metabolism of carbofuran in the resistant strain compared with the susceptible strain.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.28 no.1
• /
• pp.46-52
• /
• 2002

Purpose : We examined the relationships between the root curvatures of the mandibular third molars and their locations on the mandible, so that it might be contributed to elucidate the origin of the formation and development of the Korean mandibular third molar and help many clinicians to treat the mandibular third molars. Materials and Methods : Panoramic radiographs of 237 patients who had been treated at Korea University Ansan Hospital from March, 2000 to April, 2001 and had bilateral mandibular third molars were examined and analyzed. Results : There was no statistically significant difference between men and women (p<0.05). But there were significantly fewer subjects with bilateral curved roots in bilateral third molars regardless of their status (p<0.01) and were statistically significant differences between the groups with bilateral curved roots in the mandibular third molars with same status and with unilateral curved roots in the mandibular third molars with different status and between the groups with unilateral curved roots in the mandibular third molars with same status and with bilateral curved roots in the mandibular third molars with different status(p<0.05). Subjects with bilateral curved roots in the mandibular third molars with bilateral partial impacted status and with bilateral curved roots in the mandibular third molars with bilateral complete eruption status were significantly fewer (p<0.01) and subjects with the mandibular third molars with partial impacted and complete impacted status and with the mandibular third molars with complete erupted and partial impacted status had significantly fewer bilateral curved roots (p<0.05). Conclusions : When we postulate that it is genetic and environmental factors that affect the root formation, it seems that environmental factors have more effects on the formation of the mandibular third molars than on that of the mandibular first and second molars

• International Commerce and Information Review
• /
• v.19 no.2
• /
• pp.193-212
• /
• 2017

In order to evaluate the evaluation of bi-national products, this study was classified according to product category similarity. In addition, by comparing the types of stores, we tried to suggest alternatives that can be practically applied to companies producing composite origin products. For this purpose, this study is applied to the analysis of bi-national product by product categories, such as public luxury, public luxury, private luxury, and private necessity, based on the psychological bases of consumers. The types of stores are divided into department store/direct store, outlet/discount store to elaborate measurement. As a result of the verification, it was found that brand evaluation and store evaluation had interactive effects depending on product type and store type. Specifically, in the case of public luxury goods, outlets/discount stores in both brand evaluation and store evaluation showed positive evaluation than department store/direct store. In the case of private necessity, there was no significant difference in brand evaluation, but in store evaluation, department store/direct store was confirmed that they had a relatively positive evaluation than outlets/discount stores. As a result, it was verified that consumers' evaluations can be changed according to the type of sales store for each product.

• Journal of the Korean earth science society
• /
• v.41 no.5
• /
• pp.531-554
• /
• 2020

In order to provide explanations of the practice of the abductive inquiry-based outdoor geological fieldwork education, this study examined the characteristics of students' performance in geological fieldwork before and after the introduction of explicit learning of geologic knowledge and inquiry. To this end, a 21st-class program was developed in the order of pre-evaluation, initial fieldwork, explicit learning of geologic knowledge and inquiry, and post-evaluation and applied to nine middle school students. As research data, outdoor geological fieldwork class recording data and students' activity sheets were collected and analyzed qualitatively. As a result, during the initial fieldwork, students caught clues of low geological importance and used everyday experience and/or general scientific knowledge as a rule when asked to generate hypotheses about the origin of the clues. Also, students evaluated their hypotheses by the scientific accuracy of the rule or their own criterion. Meanwhile, during the final fieldwork, students frequently caught key clues in geologic perspectives and generated geological event hypotheses related to the clues by borrowing geologic knowledge as a rule. Furthermore, students scientifically evaluated their hypotheses based on the consistency of evidence and rules. Combining these results, the effects of learners' geological knowledge and inquiry (abduction) abilities as a path model were presented in order to help students carry out a proficient abductive inquiry in geological fieldwork.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.12
• /
• pp.1740-1745
• /
• 2009

In this study, effect of resveratrol concentration on wine taste was investigated. Content of resveratrol in 14 different wines were quantified using HPLC. The resveratrol concentrations in the Australian wine samples (grape species: Syrah) with 3 vintage years were analyzed and different concentrations of resveratrol were observed (2.89-3.84 mg/L). Variation in wine grape species with the same wine manufacturer (Chile, 2006) and variation in different manufacturing country of origin with same wine grape species (Cabernet Sauvignon, 2006) also produced the variations in resveratrol concentration. The preference analysis (color, aroma, taste,astringency and overall acceptability) of the sample wines were determined by 15 panelists using 5-point hedonic scale. When the analysis results of the resveratrol concentrations in the sample wines were compared with the preference analysis of sample wines, the resveratrol concentration in the wine produced the positive effects in the wine preference analysis in the factors of aroma, taste and color.