• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.89-93
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• 2003

human renin binding protein (hRnBp), showing N-acetylglucosamine-2-epimerase activity, was over-expressed in E. coli, but was mainly present as an inclusion body. To improve its solubility and activity, ubiquitin (Ub), thioredoxin (Trx), maltose binding protein (MBP) and NusA, were used as fusion partners. The comparative solubilities of the fusion proteins were, from most to least soluble: NusA, MBP, Trx, Ub. Only the MBP fusion did not significantly reduce the activity of hRnBp, but enhanced the stability. The Origami (DE3), permitting a more oxidative environment for the cytoplasm in E. coli; helped to increase its functional activity.

• Journal of Industrial Technology
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• v.28 no.B
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• pp.59-63
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• 2008

Rapid production of therapeutic proteins such as angiostatin and endostatin angiogenic inhibititors has been highly demanded for cancer treatment. In this regard, recombinant human angiostatin and endostatin were successfully expressed as soluble forms by maltose binding protein (MBP)-mediated fusion expression in Escherichia coli. PCR amplified, angiostatin and endostatin genes from human placenta cDNA library were inserted into an expression vector pMAL-c2e to construct prokaryotic expression vectors, pMAL-c2e/AS and pMAL-c2e/ES, respectively. Recombinant angiostatin and endostatin were efficiently expressed in E. coli origami (DE3) after IPTG induction and protein expression were confirmed by SDS-PAGE analyses. The expressed recombinant proteins were purified near homogenity using an amylose affinty column chromatography. In contrast that previous E. coli expressions were all insoluble, our results first time demonstrated that MBP fused human angiostatin and endostatin were soluble in E. coli.

• Journal of Elementary Mathematics Education in Korea
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• v.20 no.4
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• pp.695-715
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• 2016

This study is on the teaching method for the students who belong to the same school (one, the gifted class, passed gifted education of Science High school ), 1-1, face-to-face learning (two, good students in regular classroom) with a teacher, paired learning teams (4 people, gifted classes), and group lessons (20 people, gifted classes) and using the justification analysis framework tool(PIRSO) of Kim(2010) analyzes the justification element of the students in the group classes regular polygons paper was to explore ways to improve the justification of the folding maps activities. As a result, the width of the largest polygon difficulty level appropriate to the class for gifted elementary school classes but the individual learning style of the 1-1 face-to-face with a teacher or discussion with colleagues and cooperative approach is justified, rather than the material of the study of origami activities it turned out to be more effective in improving the level of justification. Unlike the individual learning activities, the exploration for class is the need to strain in parallel to the student is selected as needed, rather than serial manner was confirmed that it is necessary to clearly present problems even from the beginning. Development of teaching through the implications obtained from this method of reconstruction activities and proposed improvement measures for questioning.

• KSBB Journal
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• v.23 no.5
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• pp.403-407
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• 2008

Recently, Candida antarctica lipase B (CalB) draws attention from industries for various applications for food, detergent, fine chemical, and biodiesel, because of its characteristics as an efficient biocatalyst. Since many industrial processes carry out in organic solvent and at high temperature, CalB, which is stable under harsh condition, is in demand from many industries. In order to reform CalB promptly, the expression system which has advantages of ease to use and low cost for gene libraries screening was developed using E. coli. The E. coli strains, Rosettagami with competence for enhanced disulfide bond formation, Novablue, and $DH5{\alpha}$, were exploited in this study. To obtain the soluble CalB, the pCold I vector expressing the cloned gene at $15^{\circ}C$ and the chaperone plasmids containing groES/groEL, groES/groEL/tig, tig, dnaK/dnaJ/grpE, and dnaK/dnaJ/grpE/groES/groEL were used for coexpression of CalB and chaperones. The colonies expressing functional lipase were selected by employing the halo plate containing 1% tributyrin, and the CalB expression was confirmed by SDS-PAGE. E. coli Rosettagami and $DH5{\alpha}$ harbouring groES/groEL chaperones were able to express soluble CalB effectively. From a facilitative point of view, E. coli $DH5{\alpha}$ is more suitable for further mutation study.

• Journal of the Korean Society of Costume
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• v.57 no.2 s.111
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• pp.155-171
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• 2007

Exaggerated expressions of contemporary fashion have exceeded historical precedents in volume. The purpose of this study is to understand the aesthetic characteristics of expanded form In contemporary fashion. To take dresses beyond their flat shapes into three-dimensional forms, it was investigated into volume of dresses by using the void and the mass. The void Is rarefied space and the mass is densified space. The void of dresses could be constructed from the boning such as panier and stiff textiles as organza lining. The mass could be constructed from padding. Fold, pleats, twist, and the layering of fabrics show features of both the void and the mass. Contemporary fashion designers relied on the void rather that the mass to make the enormous volume because of the weight of the mass. X-silhouette and the expansion of skirt and sleeve were dominant as the result of accentuating the body with a waistline. However, many of expanded dresses were so voluminous that they obscured the body's outlines. Some forms were strange and shocking. Many designers explored the void and there were a multitude of methods to construct the void: compartments of air, architectural techniques, technical textiles, fold such at origami. The aesthetic consciousness of expanded dresses could be classified into 7 categories, the giant, the dynamic, the deconstructive, the historical, the comedic, the rhythmical, and the technical character.

• The Research Journal of the Costume Culture
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• v.22 no.5
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• pp.776-793
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• 2014

This study attempted to figure out the usage of the modular system in other fields through literature review and empirical study, analyze its structural method in fashion. After analyzing architecture & product-related books, the Internet data and previous studies, the modular system's five structural methods were obtained. Then, 991 photos of women's clothes from 2003 to 2014 were collected through the fashion websites in Korea and abroad. The results can be summarized as follows: First, the following five structures were derived: assembling structure, overlapping structure, arrangement structure, inserting structure and folding structure. Second, according to analysis on the structural method of the modular system in modern fashion, overlapping structure (34%) was the most common. Third, in fashion, the use of fastener for installation and removal is important for assembling structure. In terms of overlapping structure, 3D volume by vertical accumulation was commonly observed. Arrangement structure revealed a horizontal and flat shape through simple arrangement. In inserting structure, on the contrary, non-standardized modules were used as a part of clothes or decorative elements. In folding structure, origami technique for reduction and expansion was used.

• Journal of Fashion Business
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• v.19 no.5
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• pp.199-212
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• 2015

Environmentally-friendly whole-cut designs can minimize carbon dioxide emissions which are harmful to the earth, and reduce energy, labor force and time in cutting or sewing clothes. The design and way of wearing clothing will be investigated by classifying whole-cut clothing appearing in the history of costume and past traditional outfits such as Drapery, a Pancho, Tunic, or Loincloth. According to the results from the analysis of whole-cut methods applied in design, they were classified as follows: whole-cut, utilizing square-panels as is, pleats, smoking, lip band, origami, cutting way, and subtraction-cutting whole-cut design. The whole-cut design utilizing square panel as it is can minimize the waste of energy and material but can also maximize the possibility of circulation by recycling. In utilizing an all square panel, it broke away from the existing whole-cut in the western pattern, namely, the pattern of clothes clinging to the body, and was found to have new aesthetic value with a new approach. Due to the whole-cut method having a restriction in the use of dart and line cutting in its designing process, there were only designs that did not show the body line, such as designs clinging to the body. Therefore we developed a design similar to those that cling to the body by whole-cut, In addition, the work produced was with high efficiency and variability, which produces simple designs but can be worn in a variety of ways.

• Journal of Sensor Science and Technology
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• v.30 no.2
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• pp.88-93
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• 2021

In this paper, a folding sensor based on capacitance is proposed. The sensor was developed to sense the length and angle data for the milli-scale actuators without causing any interference to the actuating joints. For the sensing and testing the robotic joint with reducing the cost and complexity aspects of manufacturing, a simple composition was adopted. The sensor comprises a pair of copper tapes, papers, and wires. The complete sensing unit is constructed by bonding the tapes with the papers and soldering the wire to the copper parts. For accuracy, a teensy 4.0 board, which has a 12-bit ADC resolution, is employed. Furthermore, the sensed analog data is not translated into the unit of capacitance for accuracy; however, it is filtered using a low-pass filter and subsequently, a Butter-worth filter. The data obtained demonstrate a periodic waveform, which implies that the data are in good agreement with the hypothesis set prior to the experiments. Compared to other milli-scale sensors, this could be a better option for sensing the length and angle data for milliscale actuators.

• Journal of Aerospace System Engineering
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• v.11 no.3
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• pp.16-21
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• 2017

In this paper, we propose a new folding method (the blossom method) to increase storage efficiency of drag-sail. To resolve the issue caused by increase in the thickness of the sail, we allowed margin space (offset) along the folding line and made holes at the intersection of offset lines to prevent distortion of film. In addition, to verify applicability of the blossom method, we fabricated quarter of the sail by using Mylar film and conducted a deployment experiment. If the blossom method is applied, storage ratio (storage volume: deployed area) is 1: 68.64, that is approximately 1.88 times more than the z-fold method of folding sailing.

• Journal of the Korean Society of Costume
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• v.58 no.8
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• pp.91-105
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• 2008

Spatial multiplication phenomenon, expressed through de-constructive trend since the late of 20C, have got changed existing fixed and dis-variable space to smooth, fluid, and changeable space. Ex-form and fluidity of new spatial paradigm have been focused through lots of scientific theories studied between digital thought and indeterminacy and rankless nature phenomena. In 21C fashion, indeterminate and irregular form and space have been crested continuously, which could not explain simply according as the fluidity theory Different from the space crested for multi-function and multipurpose, this is revealed the characteristics of chaosmos that communizes the order and the disorder, deconstruction and creation. Ex-formal space of fashion have shown the relationship of de-centerizing, de-territorial, and do-structural phenomenon among different fashion elements. This paper intends to understand the concept of ex-form, and study expressive manners of ex-formal space of 21C fashion, and conclusion as follows. 1) Overlap changes single space of fashion to multi-layered space through the repeat system of pleats, origami, and folding. 2) Ex-gravity expressed in deviation of the gravity acting vertical direction, for example, twisting, curve, winding, portion. 3) Morphing is shown the change process from single fixed form to different complex form. 4) Blurring is expressed in re-combination and re-arrangement among elements of fashion. 5) Blob shows hybrid fashion space through the liberal compounding and separation of a lot of different elements.