• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.89-93
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• 2003

human renin binding protein (hRnBp), showing N-acetylglucosamine-2-epimerase activity, was over-expressed in E. coli, but was mainly present as an inclusion body. To improve its solubility and activity, ubiquitin (Ub), thioredoxin (Trx), maltose binding protein (MBP) and NusA, were used as fusion partners. The comparative solubilities of the fusion proteins were, from most to least soluble: NusA, MBP, Trx, Ub. Only the MBP fusion did not significantly reduce the activity of hRnBp, but enhanced the stability. The Origami (DE3), permitting a more oxidative environment for the cytoplasm in E. coli; helped to increase its functional activity.

• 산업기술연구
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• 제28권B호
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• pp.59-63
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• 2008

Rapid production of therapeutic proteins such as angiostatin and endostatin angiogenic inhibititors has been highly demanded for cancer treatment. In this regard, recombinant human angiostatin and endostatin were successfully expressed as soluble forms by maltose binding protein (MBP)-mediated fusion expression in Escherichia coli. PCR amplified, angiostatin and endostatin genes from human placenta cDNA library were inserted into an expression vector pMAL-c2e to construct prokaryotic expression vectors, pMAL-c2e/AS and pMAL-c2e/ES, respectively. Recombinant angiostatin and endostatin were efficiently expressed in E. coli origami (DE3) after IPTG induction and protein expression were confirmed by SDS-PAGE analyses. The expressed recombinant proteins were purified near homogenity using an amylose affinty column chromatography. In contrast that previous E. coli expressions were all insoluble, our results first time demonstrated that MBP fused human angiostatin and endostatin were soluble in E. coli.

• 한국초등수학교육학회지
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• 제20권4호
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• pp.695-715
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• 2016

본 연구는 정다각형 종이접기 활동을 소재로 한 영재교수학습 자료를 개발하고 이를 현장 수업에의 적용을 통해 발견한 교수학습 방법을 개선하는 것을 목적으로 하였다. 동일 학교에 소속한 학생들을 개별학습(1명, 발명영재학급, 과학고 영재교육원 합격), 교사와의 1-1 대면 학습(2명, 일반학급 내 우수 학생), 짝 모둠 학습(4명, 영재학급), 그리고 집단 수업(20명, 영재학급)의 여러 방식으로 유형화한 수업을 진행하면서 김정하(2010)의 정당화 분석틀(PIRSO)을 이용하여 학생들의 정당화 요소를 분석하고 집단 수업에서 정다각형 종이접기 활동의 정당화를 지도하기 위한 개선 방안을 모색하였다. 그 결과 주어진 크기의 색종이를 이용하여 최대 넓이의 정다각형 종이접기 활동 탐구라는 본 연구 소재의 난이도는 초등학교 영재학급용 수업으로 적절하였으며, 개별 학습 방식보다는 교사와의 1-1 대면 또는 동료와의 토론 및 협동 방식이 정당화의 수준을 향상시키는데 더 효과적임이 드러났다. 집단수업을 위한 탐구 활동은 모든 학생에게 모든 내용을 학습하도록 하는 일괄 수업방식보다는 필요에 따라 학생들이 개인별로 탐구하고 싶은 내용을 선택하는 선택 활동 수업 방식으로 변형할 필요가 있으며 정당화에 초점을 맞추어야 하는 과제의 목표는 처음부터 명확하게 제시할 필요가 있음을 확인하였다. 이를 바탕으로 수업의 전개나 활동의 재구성 방식, 발문을 위한 개선 방안을 제안하였다.

• KSBB Journal
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• 제23권5호
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• pp.403-407
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• 2008

본 연구에서는 현재 산업적 응용이 활발하게 이루어지고 있고, 여러 장점을 지닌 효소인 Candida antarctica에서 유래된 lipase B (CalB)의 신속한 개질을 위해 취급이 용이한 E. coli를 이용하여 CalB 발현시스템을 구축하였다. E. coli 발현 시스템에서 효소활성을 지니지 못하는 내포체를 생성하는 단점을 지니고 있어, soluble한 형태의 CalB 생성을 위해 저온 발현이 가능한 pCold I vector와 단백질 접힘을 도와주는 chaperone을 사용하여 CalB를 발현하였다. Liu 등(17)은 E. coli Origami2와 B, 그리고 $DH5{\alpha}$를 실험한 결과, Origami 균주에서만 CalB에 의한 halo의 형성이 관찰되었으나, 동 연구에서는 실험한 3종의 균주와 5종의 chaperone plasmid중 Rosettagami와 $DH5{\alpha}$에서 groES/groEL chaperone이 CalB와 동시에 발현되면 soluble한 형태의 Cal B가 발현됨을 관찰할 수 있었다. 또한 신속한 CalB의 발현시스템을 구축하기 위해서는 유전자 조작의 용이성 및 안정성에서 우월한 $DH5{\alpha}$가 Rosettagami에 비해 soluble한 CalB의 발현에 더욱 적합한 균주임이 관찰되었다. 즉 재조합 pCold plasmid와 pGro7 plasmid (groES/groEL)로 형질이 전환된 $DH5{\alpha}$가 CalB 발현시스템에 가장 적합하다.

• 복식
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• 제57권2호
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• pp.155-171
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• 2007

Exaggerated expressions of contemporary fashion have exceeded historical precedents in volume. The purpose of this study is to understand the aesthetic characteristics of expanded form In contemporary fashion. To take dresses beyond their flat shapes into three-dimensional forms, it was investigated into volume of dresses by using the void and the mass. The void Is rarefied space and the mass is densified space. The void of dresses could be constructed from the boning such as panier and stiff textiles as organza lining. The mass could be constructed from padding. Fold, pleats, twist, and the layering of fabrics show features of both the void and the mass. Contemporary fashion designers relied on the void rather that the mass to make the enormous volume because of the weight of the mass. X-silhouette and the expansion of skirt and sleeve were dominant as the result of accentuating the body with a waistline. However, many of expanded dresses were so voluminous that they obscured the body's outlines. Some forms were strange and shocking. Many designers explored the void and there were a multitude of methods to construct the void: compartments of air, architectural techniques, technical textiles, fold such at origami. The aesthetic consciousness of expanded dresses could be classified into 7 categories, the giant, the dynamic, the deconstructive, the historical, the comedic, the rhythmical, and the technical character.

• 복식문화연구
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• 제22권5호
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• pp.776-793
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• 2014

This study attempted to figure out the usage of the modular system in other fields through literature review and empirical study, analyze its structural method in fashion. After analyzing architecture & product-related books, the Internet data and previous studies, the modular system's five structural methods were obtained. Then, 991 photos of women's clothes from 2003 to 2014 were collected through the fashion websites in Korea and abroad. The results can be summarized as follows: First, the following five structures were derived: assembling structure, overlapping structure, arrangement structure, inserting structure and folding structure. Second, according to analysis on the structural method of the modular system in modern fashion, overlapping structure (34%) was the most common. Third, in fashion, the use of fastener for installation and removal is important for assembling structure. In terms of overlapping structure, 3D volume by vertical accumulation was commonly observed. Arrangement structure revealed a horizontal and flat shape through simple arrangement. In inserting structure, on the contrary, non-standardized modules were used as a part of clothes or decorative elements. In folding structure, origami technique for reduction and expansion was used.

• 패션비즈니스
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• 제19권5호
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• pp.199-212
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• 2015

Environmentally-friendly whole-cut designs can minimize carbon dioxide emissions which are harmful to the earth, and reduce energy, labor force and time in cutting or sewing clothes. The design and way of wearing clothing will be investigated by classifying whole-cut clothing appearing in the history of costume and past traditional outfits such as Drapery, a Pancho, Tunic, or Loincloth. According to the results from the analysis of whole-cut methods applied in design, they were classified as follows: whole-cut, utilizing square-panels as is, pleats, smoking, lip band, origami, cutting way, and subtraction-cutting whole-cut design. The whole-cut design utilizing square panel as it is can minimize the waste of energy and material but can also maximize the possibility of circulation by recycling. In utilizing an all square panel, it broke away from the existing whole-cut in the western pattern, namely, the pattern of clothes clinging to the body, and was found to have new aesthetic value with a new approach. Due to the whole-cut method having a restriction in the use of dart and line cutting in its designing process, there were only designs that did not show the body line, such as designs clinging to the body. Therefore we developed a design similar to those that cling to the body by whole-cut, In addition, the work produced was with high efficiency and variability, which produces simple designs but can be worn in a variety of ways.

• 센서학회지
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• 제30권2호
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• pp.88-93
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• 2021

In this paper, a folding sensor based on capacitance is proposed. The sensor was developed to sense the length and angle data for the milli-scale actuators without causing any interference to the actuating joints. For the sensing and testing the robotic joint with reducing the cost and complexity aspects of manufacturing, a simple composition was adopted. The sensor comprises a pair of copper tapes, papers, and wires. The complete sensing unit is constructed by bonding the tapes with the papers and soldering the wire to the copper parts. For accuracy, a teensy 4.0 board, which has a 12-bit ADC resolution, is employed. Furthermore, the sensed analog data is not translated into the unit of capacitance for accuracy; however, it is filtered using a low-pass filter and subsequently, a Butter-worth filter. The data obtained demonstrate a periodic waveform, which implies that the data are in good agreement with the hypothesis set prior to the experiments. Compared to other milli-scale sensors, this could be a better option for sensing the length and angle data for milliscale actuators.

• 항공우주시스템공학회지
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• 제11권3호
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• pp.16-21
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• 2017

본 논문에서는 drag-sail의 수납 효율을 높이기 위해 새로운 접기 방법인 blossom method를 제안하였다. 가항력 돛을 접을수록 두께가 늘어남에 따라 발생하는 문제점을 해결하기 위해 접히는 라인에 여유 공간(offset)을 주고, offset film 라인이 교차하는 지점에는 구멍을 뚫어 film의 일그러짐을 방지하였다. 또한, blossom method의 적용 가능성을 확인하기 위해 mylar film으로 제작하여 전개 실험을 수행하였다. Blossom method를 적용할 경우, 수납부피 대비 전개면적 비인 수납비는 1: 68.64로 기존의 가항력 돛 접기 방법인 z-fold method보다 약 1.88배 향상되었으며, 전개 실험 결과 구멍이나 jamming에 의한 손상이 없는 것을 확인하였다.

• 복식
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• 제58권8호
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• pp.91-105
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• 2008

Spatial multiplication phenomenon, expressed through de-constructive trend since the late of 20C, have got changed existing fixed and dis-variable space to smooth, fluid, and changeable space. Ex-form and fluidity of new spatial paradigm have been focused through lots of scientific theories studied between digital thought and indeterminacy and rankless nature phenomena. In 21C fashion, indeterminate and irregular form and space have been crested continuously, which could not explain simply according as the fluidity theory Different from the space crested for multi-function and multipurpose, this is revealed the characteristics of chaosmos that communizes the order and the disorder, deconstruction and creation. Ex-formal space of fashion have shown the relationship of de-centerizing, de-territorial, and do-structural phenomenon among different fashion elements. This paper intends to understand the concept of ex-form, and study expressive manners of ex-formal space of 21C fashion, and conclusion as follows. 1) Overlap changes single space of fashion to multi-layered space through the repeat system of pleats, origami, and folding. 2) Ex-gravity expressed in deviation of the gravity acting vertical direction, for example, twisting, curve, winding, portion. 3) Morphing is shown the change process from single fixed form to different complex form. 4) Blurring is expressed in re-combination and re-arrangement among elements of fashion. 5) Blob shows hybrid fashion space through the liberal compounding and separation of a lot of different elements.