• Journal of the Korean Society of Clothing and Textiles
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• v.32 no.12
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• pp.1971-1980
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• 2008

The purpose of this study is to explore the meaning and depth of traditional knitwears, and to develop knitwear designs by using Aran motif, one of major motifs of traditional knitwears and on the basis of the 'continuance' theory of Henri Bergson and Jill Deleuze. Connectivity, the sense of space and deconstructive fluidity-basic concepts of the continuance theory-are felt in such forms as pleats, origami, and air pumping systems, blob and twisting used in the modem fashion. The motif of Aran knitwear which has a long historical tradition can be reinterpreted in terms of those concepts of the continuance theory. In this study, we designed five pieces of knitwear while applying cable motif, an important motif of Aran knitwear, and the concepts of the continuance theory to them. This study will make a contribution to the designing of knit wears through reinterpretation of a traditional motif in terms of a modem philosophical thought.

• The Mathematical Education
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• v.56 no.3
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• pp.257-280
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• 2017

The purpose of this study is to analyze middle school students' learning characteristics they showed on the inquiry-based learning process of circumcenter using various teaching tools, and then to identify the effects of using teaching tools in the middle school students' learning process of circumcenter. For this purpose, we developed teaching materials for inquiry-based learning of circumcenter using textbook, origami, ruler and compass, GeoGebra and sand experiment. Then we applied them on the learning process of circumcenter for five groups of middle school students. From the analyzing of audio/video materials and documents which are collected from the process of participants' inquiry-based learning of circumcenter, we identified the following results. First, inquiry-based learning of circumcenter using various teaching tools promoted mathematical discourses among participants of this study. For example, they conjectured mathematical properties or justified their opinions after manipulated teaching tools in the process of learning circumcenter. Second, inquiry-based learning of circumcenter using various teaching tools promoted participants' divergent thinking. They tried many inquiry methods to find new mathematical properties relate to circumcenter. For example, they tried many inquiry methods to know whether there is unique circle containing four vertices of given quadrangles. Third, we found several didactic implications relate to inquiry-based learning of circumcenter using various teaching tools which are due to characteristics of teaching tools themselves. Participants showed several misconceptions about mathematical properties during they participated inquiry-based activity for learning of circumcenter using various teaching tools. We identified their misconceptions were not due to any other variables containing their learning characteristics but to characteristics of teaching tools.

• Journal of Advanced Navigation Technology
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• v.20 no.1
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• pp.99-106
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• 2016

Wearable display market is a consistently growing field to handle a smart device with ease. Wearable display is an efficient device that can show the information to the user. In this paper, propose the scheme of a wearable display using LED and implement it including controlling remotely with BLE. Traditional outdoor LED display requires the dedicated controller and its software. Therefore, to control those LED display, it should implement a driver and its own way of communication. The proposed method is to ensure the independence and extensibility by separating driver module and communication module for controlling LED display. In addition, by adopting a short-range communication with Bluetooth 4.0 and a LED driver with low-power technology, it can be showed to control system configuration and display with a smart device.

• IMMUNE NETWORK
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• v.10 no.2
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• pp.35-45
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• 2010

Background: Expression of recombinant antibodies and their derivatives fused with other functional molecules such as alkaline phosphatase in Escherichia coli is important in the development of molecular diagnostic reagents for biomedical research. Methods: We investigated the possibility of applying a well-known Fos-Jun zipper to dimerize $V_H$ and $V_L$ fragments originated from the Fab clone (SP 112) that recognizes pyruvate dehydrogenase complex-E2 (PDC-E2), and demonstrated that the functional zipFv-112 and its alkaline phosphatase fusion molecules (zipFv-AP) can be produced in the cytoplasm of Origami(DE3) trxB gor mutant E. coli strain. Results: The zipFv-AP fusion molecules exhibited higher antigen-binding signals than the zipFv up to a 10-fold under the same experimental conditions. However, conformation of the zipFv-AP seemed to be influenced by the location of an AP domain at the C-terminus of $V_H$ or $V_L$ domain [zipFv-112(H-AP) or zipFv-112(L-AP)], and inclusion of an AraC DNA binding domain at the C-terminus of VH of the zipFv-112(L-AP), termed zipFv-112(H-AD/L-AP), was also beneficial. Cytoplasmic co-expression of disulfide-binding isomerase C (DsbC) helped proper folding of the zipFv-112(H-AD/L-AP) but not significantly. Conclusion: We believe that our zipFv constructs may serve as an excellent antibody format bi-functional antibody fragments that can be produced stably in the cytoplasm of E. coli.

• KSBB Journal
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• v.24 no.4
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• pp.341-346
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• 2009

Candida Antarctica lipase A (CalA) has been used because of its suitability in industrial applications. CalA has unique features capable to accept tertiary and sterically hindered alcohols among many hydrolases. CalA gene was cloned and constructed in expression vector such as pColdIII/CalA and $pPICZ{\alpha}A$/CalA. The gene encoding pColdIII/CalA was functionally expressed in the cytoplasm of Escherichia coli $Origami^{TM}$ B (DE3) cells. The plasmid $pPICZ{\alpha}A$/CalA linearized by BstX I was integrated into 5'AOX1 region of the chromosomal DNA and was functionally expressed in the methyl atrophic yeast Pichia pastoris. Expressed CalA in P. pastoris (0.7 Unit/mL) showed 35 times higher activity than that in E. coli expression system (0.02 Unit/mL).

• Journal of the Korea Fashion and Costume Design Association
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• v.20 no.4
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• pp.95-104
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• 2018

This study classified the figurative features of the raglan sleeves presented in the Spring and Summer Collections and Fall and Winter Collections abroad in 2010 and 2018 and analyzed the production methods and patterns of the classified raglan sleeves. The analysis results are described below. The raglan sleeves in the latest fashion trends were classified into Type H, Type A, Type O and Type Y per shape. The production features of raglan sleeves in the latest fashion trends included the cutting lines in various shapes, a flounce that made shoulders look wider, and decorations such as gathers, studs, punching, slits, pleats and tucks. The raglan sleeve design was classified into Yoke Raglan, Armhole Princess Raglan, Semi Raglan, Gathered Raglan, Pleats Raglan, Cowl Raglan, Origami Raglan, Circular Curved Raglan, Capes Raglan and Constructive Design Raglan and the patterns per design were presented. For creative and experimental clothing by the analysis of the features of raglan sleeve structure, a variety of configuration methods need to be developed and implemented. The analysis results of this study will contribute to the development of the fashion industry through small quantity batch production pursuing unique styles as the basis for further study on the configuration methods of raglan sleeves. This study will be used in various ways as education materials on sleeve patterns in the educational field. Through the analysis of sleeve patterns, this study tries to provide basic data for planning the design of raglan sleeves and helping to diversify the ladies' apparel market in the future.

• Journal of the Korean Society of Clothing and Textiles
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• v.43 no.4
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• pp.579-591
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• 2019

This research investigated the needs analysis on consumers of fine dust masks to effectively develop a design prototype. Data were collected through focus group interviews on 32 individuals who have used fine dust masks. As a result, there is a new demand by consumers for technology based design changes that better fit the nose and face. Consumers preferred the smell and feel of traditional Korean paper. Young adults expressed the importance of masks that can be used for protection as well as fashion. The prototype improved the drawbacks of existing models using an origami-inspired design in a variety of colors, patterns and in sizes that are customized to fit the wearer. The proposed prototype is made of Dak felt fibers and jersey materials. It is characterized by downward-facing ear bands made of polyurethane to improve the fit around the ears and chin to more effectively block incoming air. The mask is foldable and has a crease that goes along the chin to increase the amount of enclosed space against the face making it easier to breathe compared to existing mask designs.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1732-1740
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• 2021

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and S219V to inhibit self-proteolysis. The solubility and cleavage activity of the constructed variants in Escherichia coli strains including BL21(DE3), BL21(DE3)pLys, Rossetta(DE3) and Origami(DE3) under the same induction conditions were analyzed and compared. The desirable soluble amounts, activity, and oxidative stability were identified to be reluctantly favored in the TEVp. Unlike C19S, C110S and C130S hardly impacted on decreasing protein solubility in the BL21(DE3), but they contributed to improved tolerance to the oxidative redox state in vivo and in vitro. After two fusion proteins were cleaved by purified TEVp protein containing double mutations under the oxidized redox state, the refolded disulfide-rich bovine enterokinase catalytic domain or maize peroxidase with enhanced yields were released from the regenerated amorphous cellulose via affinity absorption of the cellulose-binding module as the affinity tag.

• KSBB Journal
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• v.21 no.3
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• pp.204-211
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• 2006

For the production of the recombinant human interferon-gamma(rhIFN-${\gamma}$) in Escherichia coli, human glucagon and ferritin heavy chain were used as fusion partners. Even though rhIFN-${\gamma}$ is expressed as an inclusion body form in E. coli because of strong hydrophobicity of itself, over 50% of fused rhIFN-${\gamma}$ was expressed as soluble form in E. coli $Origami^{TM}$(DE3) harboring pT7FH(HE)-IFN-${\gamma}$ which encodes ferritin heavy chain-fused rhIFN-${\gamma}$. In the case of using glucagon-ferritin heavy chain hybrid mutant as a fusion partner, 6X His-tag was additionally introduced to N-terminus of GFHM(HE)-IFN-${\gamma}$ for enhancing purification yields of rhIFN-${\gamma}$. Fusion protein HGFHM(HE)-IFN-${\gamma}$ with two 6X His-tag was more effectively bound to Ni-NTA agarose bead than GFHM(HE)-IFN-${\gamma}$ with a 6X His-tag. rhIFN-${\gamma}$ was completely purified from enterokinase-treated HGFHM(HE)-IFN-${\gamma}$ by Ni-NTA affinity column. For high-level production of rhIFN-${\gamma}$, glucose was used as the sole carbon source with simple exponential feeding rate($2.4{\sim}7.2g/h$) in fed-batch process. The effective lactose concentration for the expression of the rhIFN-${\gamma}$ was $10{\sim}20mM$. Under the fed-batch culture conditions, rhIFN-${\gamma}$ production yield reached 11 g DCW/L for 6 hours after lactose induction.

• Journal of Korea Entertainment Industry Association
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• v.13 no.1
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• pp.199-206
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• 2019

This study aims to identify whether the action-observation training impacts on the improvement of stroke patient's cognitive functioning. When it comes to the research methods, Korean version of Mini-Mental State Examination (MMSE-K) and Literacy Independent Cognitive Assessment (LICA) conducted to assess samples between 23 April and 18 May 2018. Samples are seven patients who are hospitalized in Kyung-In region. In the meantime, seven tasks such as the range of joint motion (ROM) dance, arrangement of pullover clothes, lacing-ups of a pair, folding up a facecloth and socks, the origami and tying a necktie implemented as the action-observation programme. In order to analyse collected data, descriptive statistics analysis, paired t-test and the Wilcoxon signed-rank test were carried out via SPSS version 20 (a statistics programme). The change in value from MMSE-K showed its statistical significant as 3.29 (±1.38, p<.001) as well as value from LICA in recollective powers was 12.16 (±6.73), therefore, the statistic is said to be statistically significant. In conclusion, action-observation training most influenced recollective powers amongst stroke patient's cognitive functioning areas. Even though development of cognitive functioning discovered in other areas, its values were possibly statistically insignificant. Hence, future research ought to demonstrate which areas action-observation training is effective according to brain lesion site.