• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.130-135
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• 2006

This study was carried out to improve the analytical methods for determination of organic acids in tobacco leaf by HPLC. The samples for HPLC analysis were pre-treated by means of SPE. The calibration curve for each acid was linear and $R^2$ values ranged from 0.9990 to 1.0000. The limit of detection were obtained from the calibration curves and their values were between 1.39 to $4.87{\mu}g/mL$. Recovery rates of organic acids were between 88.6 % to 98.5 %. The concentrations of organic acids among the various tobacco leaves were compared to the concentration of organic acids, were in the order oriental, burley, flue-cured tobacco. In the case of flue-cured and oriental tobacco leaves, the order of concentration of organic acids was malic acid, oxalic acid, citric acid. But in the case of burley tobacco leaves, the order of concentration of organic acids was citric acid, oxalic acid, malic acid.

• Journal of the Korean Society of Tobacco Science
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• v.32 no.2
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• pp.77-87
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• 2010

Traditional simultaneous distillation extraction(SDE) and solid-phase micro extraction(SPME) methods using GC/MS were compared for their effectiveness in the extraction of volatile flavor compounds from different tobacco leaves types(flue-cured, burley, oriental). The major volatile flavor compounds of flue-cured and burley tobacco were similar such as neophytadiene, solanone, megastigmatrienone isomers, ${\beta}$-damascenone and ${\beta}$-ionone. On the other hand, volatile flavor compounds such as norambreinolide, sclareolide were specifically identified in oriental tobacco. Each method was used to evaluate the responses of some analytes from real samples and standards in order to provide sensitivity comparisons between two techniques. Among three types of SPME fibers such as PDMS(Polydimethylsiloxane), PA(Polyacrylate) and PDMS/DVB (Polydimethylsiloxane/Divinylbenzene) which were investigated to determine the selectivity and adsorption efficiency, PDMS/DVB fiber was selected for the extractions of the volatile flavor compounds due to its effectiveness. The qualitative analysis showed that the total amount of volatile flavor compounds in SDE method(130 species) was much more than that in SPME method(85 species). SPME method was more efficient for all the highly volatile compounds than SDE method, but on the other hand, low-volatile compounds such as fatty acids or high-molecular hydrocabons were detected in SDE method. SPME method based on a short-time sampling can be adjusted to favor a selected group compounds in tobacco. Furthermore this results could be used to estimate the aroma characteristics of cigarette blending by using a different type of tobacco with more effectiveness and convenience.

• Journal of the Korean Society of Tobacco Science
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• v.10 no.2
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• pp.109-116
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• 1988

Oriental tobacco (KA 101) was transplanted from Mar. 25 to May 5 with 10 days interval in 1984 and 1985, and its agronomic characteristics, chemical properties ware compared to determine the Proper transplanting time of aromatic tobacco In Korea. As the transplanting was delayed, days to flowering of plant was shortened, length and width of largest leaf, leaf area index tended to decrease. Yield was highest for the Apr. 5th transplanting followed by Mar. 25th planting, then decreased as the planting date was delayed. Quality by price decreased as the transplanting was delayed later than Apr. 25. Delaying transplanting increased nicotine, total nitrogen and ash content, but decreased reducing sugar and petroleum ether extract of cured leaves, The content of volatile organic acids such as 3-methyl pentanoic acid was lower when it was transplanted later than Apr. 25th. Neophytadiene content increased as the transplanting was delayed, but there were no trends with the content of alcohols, aldehydes esters and ketones. Several quality indices including the ratio between the content of volatile organic acids plus petroleum ether extract and ash content plus pH was higher for the Apr. 5th transplanting.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.1
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• pp.90-97
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• 1993

1. Average moisture content of tobacco in BIB silo was about 19: 1% until 4hours conditioned time, and then moisture variation of tobacco after 2hr conditioned was very small. 2. Application of mathematical model for ordering system. 1) The constant K in the exponential equation varies inversely with both relative humidity and equilibrium moisture. 2) Time needed to order blending tobacco leaves with standard moisture from bulking and blending silo was 4 hours. 3) Reconstituted tobacco sheet had higher moisture absorption ratio than Oriental and Burley tobacco. 4) For minimize of conditioning time in BIB silo, the values of K and Mo given in this study can be used in equation(1) to calculate moisture absorption ratio and optimum conditioning time. 3. Average moisture content and water activity of conditioned tobacco for 4 hours in BIB silo was about 20% and 0.65. In this condition. microbial life will inhibite 4. Physical properties of conditioned tobacco in bulking and blending silo for 4hours was virtually no change.

• Journal of the Korean Society of Tobacco Science
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• v.6 no.2
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• pp.207-214
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• 1984

In order to obtain the basic information for the successful breeding of aromatic tobacco (N. tabacum L.), the combining ability and the mode of inheritance in 28 $F_2$ hybrids by diallel crosses between Korea local and oriental varieties were investigated. Estimates of general combining ability were significant for all characters investigated, and the specific combining ability was significant for stalk height, leaves per plant, leaf length, leaf width and days to flower. The partial dominance was exhibited by all characters investigated as well as additive effects were larger than dominance effects for all characters.

• Journal of the Korean Society of Tobacco Science
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• v.6 no.1
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• pp.3-11
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• 1984

In order to obtain the basic information for the successful breeding of aromatic tobacco (Nicotiana tabacum L.), the heterosis, combining ability and gene distributions in 28 Fl hybrids by diallel crosses between Korea local and Oriental varieties were investigated. The yield were higher, stalk height, leaf length and leaf width were larger and days to flower was shorter for the Fl hybrids when compared to mid-parent. Estimates of general combining ability were significant for all characters investigated, and specific combining ability were significant for yield, stalk height, leaves Per Plant, leaf width and days to flower. In vr-wr graph, partial dominane was exhibited by all characters investigated.

• Korean journal of applied entomology
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• v.28 no.2
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• pp.88-92
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• 1989

The Oriental tobacco budworm, Heliothis assulta (Guenee), larvae usually feed on the hot-pepper fruits and young tobacco leaves or buds in Korea. This experiment was conducted t(} compare their attraction to their host plants including fruits of the sweet-pepper and solanum uk kwang, besides the other two mentioned above. First instar larvae were most attracted to the hot-pepper fruits. Flowers or leaves of the hot pepper plants were not attractive either. When the hot-pepper fruits were compared for attractancy, based on developmental stages, relatively mature fruits, just before ripening, were the most attractive. Their extract with or without 10% ethanol also gave similar result to 1st instar larvae or 2~4 instar larvae, suggesting a possibility that there is an attractant(s) in the hot-pepper fruits.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.465-472
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• 2014

The tobacco whitefly, Bemisia tabaci, infest the Oriental melon and give significant economic damage along with its virus-vectoring activity. Various biotypes of B. tabaci have been well known and are classified depending on the severity of crop damage and insecticide susceptibility. In this study, B. tabaci adults were collected in the melon fields located in Poongchun-myeon, Andong, Korea and diagnosed on their biotypes using PCR molecular markers. From the all the 11 greenhouses, B. tabaci biotype Q was identified. In addition, biotype B adults were also found from the 4 greenhouses. These results report the first occurrence of B. tabaci at the Oriental melon farms in Gyeongbuk province with mixed infection by the two biotypes in the area.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.15-20
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• 1982

This experiment was carried out to investigate the degrees and directions of dominance, and gene distributions by analysing diallel crosses of oriental varieties. The analysis of Wr-Vr indicated non-allelic gene interaction for days to (lowering and number of leaves. Five Plant characteristics showed different degrees of dominance : incomplete dominance for Plant height and leaf shape, over dominance for , wield and sugar, and complete dominance for nicotine. It was shown that additive genetic variance was predominant for plant height, leaf shape, and total sugar. More number of dominant genes were present in Kavala and canthi for Plant height; Basma, Samsun and Izmir for leaf shape ; and Basma and Samsun (or nicotine. Their directions of dominance were tall height, narrow leaves, and low nicotine, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.132-139
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• 2007

This study was performed to apply the more rapid and accurate sample preparation, and the high selectivity and sensitivity of the analyte detection by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). QuEChERS (quick, easy, cheap, effective, rugged and safe) method was validated for 49 agrochemicals in the CORESTA Agrochemical Advisory Committee guide and amenable to GC-MS/MS determination. In QuEChERS method, the effects of sorbents (PSA, $C_{18}$ and GCB) and matrix of the analytes in tobacco types (flue-cured, burley and oriental) were investigated. MS/MS acquisition provided high specificity and selectivity for agrochemicals and low limit of quantification. QuEChERS by using PSA alone and the matrix-matched standards gave good recoveries and RSD values in three types of tobaccos. QuEChERS method was no needed to be complex clean-up procedure and would be used as the fast and easy method for agrochemical residue analysis in tobacco.