• Korean Journal of Pharmacognosy
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• v.45 no.4
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• pp.282-287
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• 2014

The ORI2 (3-[3,4-dihydroxyphenyl]acrylic acid 1-[3,4-dihydroxyphenyl]-2-methoxycarbonylethyl ester) was purified from the extract of Isodon excisus. We confirmed the antiviral effect of ORI2 in a coxsackievirus-induced pancreatitis model. Coxsackievirus B4 (CVB4) is a common cause of pancreatitis and may be reason of the type-1 diabetes. Anti-enteroviral compounds were screened by HeLa cell survival assay. Purified natural compounds were added to HeLa cells cultured 96-well plates after $10^4PFU/ml$ CVB4 pre-incubation for 30 min. ORI2 significantly improved HeLa cell survival in a dose-dependent manner. In addition, ORI2 (1 mM) treatment was dramatically decreased virus protease 2A induced eIF4G-I cleavage and viral VP1 capsid protein production. HeLa cell virus titers and viral RNA replication were significantly decreased in ORI2-treatment in a dose dependent manner (1 mM~0.001 mM). These results demonstrate that ORI2 has a strong antiviral effect. It was significantly decreased virus replication. ORI2 may be developed as a potential therapeutic agent for CVB4.

• Korean Journal of Microbiology
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• v.32 no.3
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• pp.186-191
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• 1994

The origin of the leading strand replication (ori) and of lagging strand replication (M-O) of R-plasmid pSBK203 was identified and its base sequence was determined. About 50 bp of ori sequence residues overlapped with the structural gene of rep. Sequence comparison reveals that pSBK-ori shares obvious identities with those of pT181 family and consists of two regions, one is conserved and the other is variable region. Of two palindrome sequence located one after another in upstream region of rep gene, palA' instead of palA which shares sequence homology with diverse family of plasmids such as pOX6, pC194, and pE194 seems to act as a signal for conversion of primarily replicated ssDNA to dsDNA (minus origin (M-O)).

• BMB Reports
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• v.43 no.11
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• pp.744-749
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• 2010

The aim of this work was to determine whether intrinsically bent DNA sites are present at, or close to, the mammalian replication origins oriGNAI3 and oriB in the Chinese hamster AMPD2 locus. Using an electrophoretic mobility shift assay and in silico analysis, we located four intrinsically bent DNA sites (b1 to b4) in a fragment that contains the oriGNAI3 and one site (b5) proximal to oriB. The helical parameters show that each bent DNA site is curved in a left-handed superhelical writhe. A 2D projection of 3D fragment trajectories revealed that oriGNAI3 is located in a relatively straight segment flanked by bent sites b1 and b2, which map in previously identified Scaffold/Matrix Attachment Region. Sites b3 and b4 are located approximately 2 kb downstream and force the fragment into a strong closed loop structure. The b5 site is also located in an S/MAR that is found just downstream of oriB.

• Korean Journal of Pharmacognosy
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• v.42 no.3
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• pp.209-212
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• 2011

Korean folk medicine 'ORiNaMu' has been used mainly to cure toothache and alcoholism. With regard to the botanical origin of 'ORiNaMu', it has been considered to designate the branch of Alnus species (Betulaceae), but there was no pharmacognostical confirmation on it. To clarify the botanical origin of 'ORiNaMu', the anatomical characteristics of the branch of Alnus firma, A. hirsuta, A. japonica and A. maximowiczii were studied. As a result, it was found the morphological criteria for the four Alnus species that could discriminate them the number of cork cell layer, fiber, stone cell and diameter of vessel. According to there criteria, it was clarified that the commercial folk medicine 'ORiNaMu' was the branch of Alnus firma.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.4
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• pp.299-315
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• 2020

Tuna fisheries were applied to an integrated ecosystem-based fishery risk assessment method using indexes of target species status, inhabited species in a target ecosystem, habitat quality and socio-economic benefit of affected fisheries. This study suggested more effective and efficient management measures to break away from traditional management methods, such as limitation of catch and fishing effort. The results presented that the objective risk index (ORI_S) on sustainability of bigeye and yellowfin tunas by purse seine fishery was estimated high due to the high catch ratio of small fishes. The ORIs of biodiversity (ORI_B) and habitat quality (ORI_H) of purse seine fishery were also estimated at a high level from using fish-aggregating devices (FAD). However, due to skipjack tuna's high catches, the ORI of socio-economic benefit (ORI_E) was estimated at a very low level. Due to the high bycatch rate, ORI_B was high, and ORI_S and ORI_H were evaluated at a low level in longline fishery. Due to strengthern of fishing restrictions and increase of fishing costs, the ORI_E was assessed to be very high. The ecosystem risk index (ERI) for two tuna fisheries was assessed low, but the overall FAD management by purse seine fishery is necessary at the ecosystem level.

• BMB Reports
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• v.38 no.1
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• pp.97-103
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• 2005

Under the condition of expression of $\lambda$ P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the $\lambda$ P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the $\lambda$ P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of $\lambda$ P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.144.2-144.2
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• 2012

접촉 식쌍성 FZ Ori를 CCD 측광 관측하여 BVRI 광도곡선을 얻고, 12개의 새로운 극심시각을 결정하였다. 먼저 우리가 구한 극심시각들을 포함한 총 218개의 극심시각 자료를 이용하여 FZ Ori의 공전주기 변화를 분석하였다. 그 결과 지난 80년 동안 FZ Ori의 공전주기가 영년 증가하면서 동시에 40~50년 주기로 변화하고 있음을 발견하였다. 우리는 주기적 변화가 제3천체에 의한 광시간 효과로 나타난다고 가정하고 몬테카를로 기법을 이용하여 $X^2_r$인 자 공간에서 최적의 광시간 해를 탐색하였다. 또 이 방법으로 구한 광시간 궤도요소를 궤도 수치적분 프로그램(MERCURY, S34BODY)에 적용하여 질점으로 이루어진 가상의 삼중성계에서 나타나는 광시간 효과를 천체역학적으로 구현하여 보았다. 한편 FZ Ori의 4색 광도곡선을 가장 최근에 개정된 2010 Wilson-Devinney 프로그램에 적용하여 측광학적 해를 구하였다. 기본 시스템 인자 외에 차가운 흑점과 뜨거운 흑점, 그리고 제3광도의 세 요인들을 조합하여 구한 14개의 해 중에서 주성과 반성의 표면에 각각 차가운 흑점과 뜨거운 흑점이 있으면서 제3광도가 검출된 해가 우리의 관측과 가장 잘 맞았다. 그러나 이렇게 구한 제3광도는 광시간 모형으로부터 예측되는 제3천체의 광도에 크게 못 미친다. 추후 분광 관측을 비롯한 다양한 방법을 동원한다면 FZ Ori에 대한 보다 완전한 모형을 얻을 수 있을 것이다.

• Animal cells and systems
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• v.2 no.3
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• pp.351-353
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• 1998

A 245 bp segment of E. coli chromosomal replication origin, oriC, contains 11 repeats of the GATC sequence in which adenine is methylated by Dam methylase. Newly replicated oriC is hemimethylated. The parental strand of the newly replicated oriC is methylated, but the nascent strand is not yet methylated until methylated by Dam methylase. The hemimethylated oriC plays an important role in the regulation of chromosomal replication. Activity in the seqA mutant was identified to bind preferentially to hemimethylated DNA, but not to fully-methylated DNA. This activity may participate in the sequestration of initiation of chromosomal replication.

• Electronics and Telecommunications Trends
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• v.28 no.3
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• pp.106-114
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• 2013

과거 이동통신용 기지국은 기저대역 처리부와 안테나를 포함하는 RF부가 일체형으로 제작되었으나, 4세대 이동통신에서는 기술/경제적인 이유로 BBU(Base Band Unit)와 RRH(Remote Radio Head)로 분리하여 구성되는 분산형 기지국 구조를 택하는 추세이다. BBU와 RRH 간 인터페이스로 널리 사용되어온 OBSAI, CPRI외에 2010년 ORI가 출현하여 표준화가 한창이다. ORI는 시장에서 널리 사용되는 CPRI(Common Public Radio Interface)의 Vendor Specific 부분으로 인해 제조사간 호환성이 보장되기 어려운 문제를 보완하기 위하여 탄생한 인터페이스 규격으로써, CPRI를 기반으로 하면서 표준으로써의 완성도를 높인 표준이라 할 수 있다. 또한 관련 표준으로는 유일하게 IQ 데이터 압축알고리즘의 표준화를 고려하고 있다. 본고에서는 ORI의 표준화 현황과 기술적인 이슈를 소개하고, 국내의 대응 현황을 알아본다.

• Korean Journal of Microbiology
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• v.25 no.4
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• pp.262-273
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• 1987

The replication region of the chloramphenical resistance plasmid pSBK203 of Staphylococcus aureus was cloned using pBR322 and pBD9 as vectors. Cloned replication tegion and chloramphenicol resistance gene were recombined to pBR322. The reconstructed vector behaved as a shuttle vector for E. coli and B. subtilis.