• Food Science and Preservation
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• v.21 no.1
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• pp.91-96
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• 2014

Evidence suggests that dietary indole, particularly rich in cruciferous vegetables, may reduce the risk of cardiovascular diseases. Endosulfan is a residual organochlorine pesticide, which is detected in fruits, vegetables, and crops. In this study, we investigated the effect of luminal indole on endosulfan transport in the small intestine in mesenteric lymph duct-cannulated rats. The duodenum was also cannulated and a fasting phosphate buffered saline-glucose solution was infused overnight at 3 mL/hr. After recovery, a lipid emulsion containing [2,3-$^{14}C$] endosulfan ($^{14}C$-endosulfan) was infused into the duodenum for 8 hours. The tested rats were infused with the same lipid emulsion, but with indole. Samples from the lymph-fistula were collected hourly, and the luminal contents and mucosa were collected at the end of the infusion. The lymph flow in the mesenteric lymph did not differ between the two groups. However, the intestinal absorption of both endosulfan and cholesterol were significantly decreased by indole. The amount of radioactive endosulfan, which remained in the mucosa, was greater in the indole-infused rats due to the decreased transport of endosulfan into the lymph. This study indicates that the indole decreases the intestinal transport of endosulfan into the mesenteric lymph.

• Journal of the Korean Chemical Society
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• v.39 no.12
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• pp.902-909
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• 1995

Detection limit as well as calibration curves on organophosphorus pesticide(dimethoate, diazinon, parathion-methyl, fenitrothion, malathion) and BHCs were measured for evaluation of utility on qualitative or quantitative analysis of pesticides with ion Trap mass spectrometer and quadrupole mass spectrometer. As ionization source, EI and CI were adopted for qualitative analysis of pesticides by comparison of each fragmentation pattern. At the same time, the utility as trace analysis techniques through scan or selected ion monitoring(SIM) mode was evaluated. With ion trap for all pesticides, detection limit(DL, 1 ${\mu}L$ injection) on scan mode was ranged 0.008∼0.225 ng at signal to noise ratio 3. With quadrupole DL on scan mode was ranged 0.23∼3.1 ng over 0.032∼0.68 ng on SIM mode. The calibration curve with ion trap generated good linearity over 0.99 as correlation coefficient. As clean up procedure, Bio Beads S-X3 was used for the separation of oils from five organophosphorous pesticides in flour extractant showing more than 80% as recovery at most cases. In case of BHCs in jinseng with Florisil column, the recovery of pesticides has been 60% to 90%.

• Journal of Life Science
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• v.32 no.10
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• pp.771-777
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• 2022

Dopaminergic (DA) cell death in Parkinson's disease (PD) has been attributed to multiple, distinct genetic and environmental factors. In rare familial PD loss of parkin function mutations play a key role in nigral DA neuron-specific pathogenesis primarily via endoplasmic reticulum (ER) stress. In more prevalent sporadic PD, environmental exposure to pesticides has a significant epidemiological role. However, it is largely unknown how environmental exposure to xenobiotics is etiologically linked with the known etiology in familial PD. In the present study biochemical evidence for a common pathogenic mechanism between sporadic and familial PD has been identified employing the recently characterized mesencephalic DA cell line, N27-A. Dieldrin, an organochlorine pesticide epidemiologically implicated in sporadic PD, induced the markers of ER stress response such as a chaperone BiP/Grp78, heme oxygenase-1 and especially, parkin. Accordingly, dieldrin activated the ER resident Caspase-12, a mediator of ER stress-specific apoptosis, during cell death of N27-A cells. Of great interest the dieldrin-induced DA neuronal cell death was synergistically rescued by the overexpression of ER resident neuroprotective proteins, parkin and Bcl-2. The present findings implicate that accumulation of ER stress could be one of common pathogenic mechanisms in idiopathic and familial PD, and some ER proteins, such as parkin and Bcl-2 may effectively attenuate ER stress-mediated N27-A DA cell death.