• Title/Summary/Keyword: organic antifungal agent

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Antifungal Activities of Isothiazoline/Cabamate based Organic Antifungal Agent Activated-Cement Mortars (AACM)

  • Do Jeong-Yun;So Hyoung-Seok;Soh Yang-Seob
    • KCI Concrete Journal
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    • v.14 no.4
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    • pp.171-177
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    • 2002
  • Antifungal agents are used to impart antibacterial or bactericidal properties to commodities and various articles used in industries and can be classified into two broad groups i.e organic and inorganic. Inorganic antifungal agents comprise of Ag, Zn, or Cu, etc. These elements tend to exhibit high level of antifungal activities, non-uniform dispersion in substrates, and have poor properties in expensive and cheap adhesiveness. In this study, the organic antifungal agent was used for the purpose of investigating the antifungal activity of antifungal agent activated-cement mortar (AACM) on the aspergilus niger of various fungus which can be easily discovered in the interiors and exteriors of buildings. In addition, an experiment on the basic physical properties of AACM such as compressive and flexural strength was carried out. The conclusion of this investigation revealed that a dosage increase of antifungal agent exhibits a high inhibitory effect on the aspergilus niger, and although there is a slight decrease in the strength of AACM, the strength of AACM was almost equal to that of inactivated cement mortar.

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Biocontrol Traits and Antagonistic Potential of Bacillus amyloliquefaciens Strain NJZJSB3 Against Sclerotinia sclerotiorum, a Causal Agent of Canola Stem Rot

  • Wu, Yuncheng;Yuan, Jun;Raza, Waseem;Shen, Qirong;Huang, Qiwei
    • Journal of Microbiology and Biotechnology
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    • v.24 no.10
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    • pp.1327-1336
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    • 2014
  • Bacillus amyloliquefaciens strain NJZJSB3 has shown antagonism of several phytopathogens in vitro, especially Sclerotinia sclerotiorum. Both the broth culture and cell suspension of strain NJZJSB3 could completely protect the detached leaves of canola (Brassica napus) from S. sclerotiorum infection. In pot experiments, the application of strain NJZJSB3 cell suspension ($10^8CFU/ml$) decreased the disease incidence by 83.3%, a result similar to commercially available fungicide (Dimetachlone). In order to investigate the potential biocontrol mechanisms of strain NJZJSB3, the nonvolatile antifungal compounds it produces were identified as iturin homologs using HPLC-ESI-MS. Antifungal volatile organic compounds were identified by gas chromatography-mass spectrometry. The detected volatiles toluene, phenol, and benzothiazole showed antifungal effects against S. sclerotiorum in chemical control experiments. Strain NJZJSB3 also produced biofilm, siderophores and cell-wall-degrading enzymes (protease and ${\beta}$-1,3-glucanase). These results suggest that strain NJZJSB3 can be a tremendous potential agent for the biological control of sclerotinia stem rot.

Antifungal and Insecticidal Activity of Methanol Extracts from 17 Medicinal Plants against Biological Deterioration of Organic Cultural Heritage (약용식물 17종 메탄올 추출물의 유기질문화재 생물학적 열화원에 대한 항진균 및 살충활성 조사)

  • Lim, Jin-A;Choi, Yun-A;Jung, Mi-Hwa;Kang, So-Yeong;Chung, Yong-Jae
    • 보존과학연구
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    • s.29
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    • pp.125-136
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    • 2008
  • To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, methanol extracts from 17 medicinal plants were screened for antifungal and insecticidal activity against 6 wood rot fungi and adult of Lasioderma serricorne. Antifungal activity of extracts was tested by using paper disc soaking method against wood-rot fungi. Among these extracts, the most significant antimicrobial activity was observed from the extract of Coptis japonica at 5 mg/disc against all wood rot fungi tested. The insecticidal activity of extracts was examined by topical application against L. serricorne adults. The extracts from Asarum sieboldii gave 83.3 % mortality at $25{\mu}g/adult$ for 24hr. From these result, the methanol extract from C. japonica and A. sieboldii described could be useful for conservation of organic cultural heritage against biological deterioration by wood rot fungi and L. serricorne.

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Antifungal and Insecticidal Activity of Essential Oil from Asarum sieboldii against Wood Contaminant Fungi and Lasioderma serricorne L. (세신 정유추출물의 목재부후균과 궐련벌레에 대한 항진균 및 살충활성)

  • Kang, Soyeong;Chung, Yong Jae;Lim, Jin A
    • Journal of Conservation Science
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    • v.28 no.4
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    • pp.395-401
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    • 2012
  • To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, essential oil from Asarum sieboldii was screened for antifungal and insecticidal activity against 4 wood rotting fungi and adult of Lasioderma serricorne. Antifungal activity of essential oil was tested by using paper disc soaking method. Antifungal activity expressed as $IC_{50}$ value showed $1.50{\sim}2.84{\mu}l/disc$ range and the most significant antifungal activity was observed in Lentinus lepideus. The insecticidal activity of essential oil was examined by topical application method against L. serricorne adults. 50% and 100% of essential oil gave 98.3% and 100% mortality for 24 hours, respectively. The major components of the essential oil were methyl eugenol (56.32%), eucarvone (11.53%), safrole (5.79%), ${\delta}$-3-carene (2.09%), which were identified by gas chromatography-mass spectrometry. From these results, essential oil from A. sieboldii could be useful for conservation of organic cultural heritage against biological deterioration by insect and wood rotting fungi.

Development and Application of Natural Antimicrobial Agent Isolated from Grapefruit Seed Extract (Grapefruit 종자추출물로부터 광범위 항균제 개발 및 응용에 관한 연구)

  • 조성환;이상열;김재원;고경혁;서일원
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.33-39
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    • 1995
  • The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed coparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

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Synthesis and Biological Valutaion of New 5-Fluorobenzimidazole Antifungal Agents (새로운 5-Fluorobenzimidazole 항진균제의 합성과 생물학적 평가)

  • Ryu, Soung-Ryual
    • Journal of the Korean Applied Science and Technology
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    • v.28 no.1
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    • pp.118-125
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    • 2011
  • New type of 5-fluorobenzimidazole derivatives was synthesized through the reaction of 4-fluoro-5-(2,6-dimethylmorpholinyl)-2-aminoaniline with 5-nitro-2-furoic acid and 5-methoxy-3-chlorobenzothiophene-2-carboxylic acid in presence of PPA and treatment of $OH^-$. the resulting substituted 5-fluorobenzimidazole derivatives(6), (7) was characterized by high solubility in common polar organic solvents. We considered 5-fluorobenzimidazole derivatives were useful especially for antifungal drugs. These results are discussed from the viewpoints of the chemical and physical structures of the 5-fluorobenzimidazole derivatives.

Structure-Based Virtual Screening and Biological Evaluation of Non-Azole Antifungal Agent

  • Lee, Joo-Youn;Nam, Ky-Youb;Min, Yong-Ki;Park, Chan-Koo;Lee, Hyun-Gul;Kim, Bum-Tae;No, Kyoung-Tai
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2005.09a
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    • pp.139-143
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    • 2005
  • Cytochrome P450 14${\alpha}$-sterol demethylase enzyme (CYP51) is the target a of azole type antifungals. The azole blocks the ergosterol synthesis and thereby inhibits fungal growth. A three-dimensional (3D) homology model of CYP51 from Candida albicans was constructed based on the X-ray crystal structure of CYP51 from Mycobacterium tuberculosis. Using this model, the binding modes for the substrate (24-methylene-24, 25-dihydrolanosterol) and the known inhibitors (fluconazole, voriconazole, oxiconazole, miconazole) were predicted from docking. Virtual screening was performed employing Structure Based Focusing (SBF). In this procedure, the pharmacophore models for database search were generated from the protein-ligands interactions each other. The initial structure-based virtual screening selected 15 compounds from a commercial available 3D database of approximately 50,000 molecule library, Being evaluated by a cell-based assay, 5 compounds were further identified as the potent inhibitors of Candida albicans CYP51 (CACYP51) with low minimal inhibitory concentration (MIC) range. BMD-09-01${\sim}$BMD-09-04 MIC range was 0.5 ${\mu}$g/ml and BMD-09-05 was 1 ${\mu}$g/ml. These new inhibitors provide a basis for some non-azole antifungal rational design of new, and more efficacious antifungal agents.

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Biological Characterization of Paenibacillus polymyxa JE201 with Antifungal Activity Against Fungal Leaf Spot Disease of Aster scaber (취나물 점무늬병을 억제하는 Paenibacillus polymyxa JE201의 생물학적 특성)

  • Ahn, Seong-Ho;Kim, Dayeon;Park, Byeng-Yong;Han, Ji Hee;Lee, Sang-Yeop
    • Korean Journal of Organic Agriculture
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    • v.29 no.2
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    • pp.257-273
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    • 2021
  • Koreans consume cham-chwi (Aster scaber thunb.) as a common vegetable in a meal because of its bitter taste and rich flavor. In addition, it is the crop with the most residual pesticides detected in the last five years. Among the detected pesticides, the most common was azoxystrobin, which is a drug used primarily to prevent the leaf spot disease of A. scaber caused by Septoria sp.. We isolated the microorganisms that antifungal activity against Septoria sp.. The optimum incubation conditions (temperature, pH and growth medium) were examined for the growth of the isolates. Additionally, cellulase and protease activity and siderophore production ability were also examined. According to 16S rRNA sequencing of the isolate was affiliated to Paenibacillus polymyxa JE201. Largest inhibition zone measuring up to 9.2 mm was observed for P. polymyxa JE201 after 7 days of inoculation. P. polymyxa JE201 strain showed antifungal activity against various fungal phytopathogens Altanaria sp., Botrytis cinerea, Colletotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Ph. drechesleria, Rhizoctonia solani and Stemphylium sp.. Based on these observations, P. polymyxa. JE201 can be used as a promising biocontrol agent for preventing the leaf spot disease and other phytopathogens.

Antifungal and Insecticidal Activity of Methanol Extract from 11 Korean Wood Species (국내산 수목 추출물의 항진균 및 살충활성)

  • Lim, Jin-A;Choi, Yun-A;Jung, Mi-Hwa;Kang, So-Yeong;Chung, Yong-Jae
    • Journal of Conservation Science
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    • v.23
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    • pp.95-102
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    • 2008
  • To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, methanol extracts from 11 wood species which populate in Korea were screened for antifungal and insecticidal activity against 9 wood contaminant fungi and adult of Lasioderma serricorne. Antifungal activity of extracts was tested by using paper disc soaking method and feeder strip test against wood-rot fungi and wood surface contaminant fungi. Among these extracts, the most significant antimicrobial activity was observed from the extract of Phellodendron amurense at 5 mg/disc against Coniophora puteana, Lentinus lepideus, Tyromyces palustris and Aspergillus niger. The insecticidal activity of extracts was examined by topical application against L. serricorne adults. The extracts from Taxus cuspidata and Abies holophylla gave 56.7 % mortality at 25 ${\mu}g$/adult for 24 hr. From these result, the methanol extract from T. cuspidata, A. holophylla and P. amurense described could be useful for conservation of organic cultural heritage against biological deterioration by insect and wood contaminant fungi.

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Falcarindiol, a Polyacetylenic Compound Isolated from Peucedanum japonicum, Inhibits Mammalian DNA Topoisomerase I

  • Lee, Gwang;Park, Hyoung-Gun;Choi, Mi-Lim;Kim, Young-Ho;Park, Yong-Bok;Song, Kyung-Sik;Cheong, Chaejoon;Bae, Young-Seuk
    • Journal of Microbiology and Biotechnology
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    • v.10 no.3
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    • pp.394-398
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    • 2000
  • A methanol extract of the root of Peucedanum japonicum, used as a medicinal herb, showed an inhibitory effect on mammalian topoisomerase I activity. The methanol extract was suspended in ethyl acetate, and a topoisomerase I inhibitor in the organic soluble fraction was then isolated by silica gel and thin layer chromatography. The topoisomerase I inhibitory compound was indentified as falcarindiol based on the analysis of EI-MS, $^1$H and \ulcornerC NMR spectroscopy. This inhibitory showed cytotoxicity against human leukemia Jurkat T and HL60 cells with an IC\ulcorner value of 7 $\mu\textrm{g}$/ml. These results suggest the possibility of falcarindiol as a new anticancer agent which can be expected to have a synergistic effect on other anticancer drugs. In addition, the present data show that falcarindiol has antifungal, yet not antibacterial, activity.

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