• 비교문화연구
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• 제26권
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• pp.227-248
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• 2012

Orchid Dream Wonderful Biography(蘭花夢奇傳), whose author was Yimmeishanren(吟梅山人), is a Xia-Yi-Ai-Qing(俠義愛情) novel in the late Qing Dynasty. The work published in 1905 when Emperor Guangxu(光緖) governed China. The novel was reprinted for several times to its excellent art feature as well as its public praise. Orchid Dream Wonderful Biography narratives the life story of the heroin, Songbaozhu(松寶珠). Songbaozhu disguises herself as a man, takes the imperial examination, and takes part in the war. However, she is abused after marring Xuwenqing(許文卿), finally, she dies in misery. Songbaozhu's tragic fate show that although a woman may disguise as a man and makes great contributions in novel, it would never come true in reality. Songbaolin(松寶林) is also home to female hero. She has a younger brother and sister were strictly education. She is a female hero of the pitch. The writer creates her, had to satisfy a social reality male. Orchid Dream Wonderful Biography, this work has had a tragic or a reality. This work represents the limits of consciousness and the writers. Orchid Dream Wonderful Biography did not transcend male-oriented notions.

• 한국육종학회지
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• 제41권4호
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• pp.510-514
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• 2009

난 시장이 확대되고 있지만 일반대중이 선호하고 있는 동양계 심비디움 난은 대부분 수입 또는 산채에 의존하여 고가로 판매되고 있고, 서양계 심비디움은 외국 품종에 대한 로열티 지불 등의 문제가 대두되고 있다. 본 연구에서는 관상가치가 있고 대량증식이 가능하여 외국산 품종을 대체할 수 있는 소형 심비디움 난을 육성하고자 하였다. 동양계 및 서양계 심 비디움의 교배종인 "동양"의 바이러스 무병주를 생장점배양에 의해 기내에서 대량증식을 하던 중, 잎무늬 돌연변이 식물체를 선발하여 고정계통을 "대국"이라 명명하였다. "대국"은 잎의 가장자리에 백색이나 노란색 줄무늬가 있는 복륜 무늬종으로 광택이 있다. "대국"의 잎 길이는 약 40 cm, 엽폭은 1.6 cm, 엽수는 4~5장으로 동양에 비교하여 잎 및 초세가 작고 단아하다. 꽃에도 복륜무늬가 있으며 개화시기는 3~5월과 9~10월 연 2회이다. 신품종 "대국"은 잎무늬 줄무늬와 초세가 단정하여 분화용 엽예품으로 관상가치가 뛰어나며, 기존 동양계 심비디움 품종에 비교하여 조직배양 및 순화가 잘되고 생육이 왕성하여 값싸게 공급 가능한 품종이다.(품종특허 등록번호: 특허 제 0478734).

• Journal of Plant Biotechnology
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• 제50권
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• pp.108-114
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• 2023

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative Mixture^TM (PPM^TM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPM^TM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPM^TM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPM^TM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPM^TM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.357-369
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• 2010

Cymbidium is horticulturally important and has been one of the most commercially successful orchid plants as well as cut flowers around the world including Korea. Up to now, a huge number of elite Cymbidium cultivars have been released on the commercial market via cross-hybridization, mutation and polyploidization breeding techniques. To investigate on breeding system in Cymbidium, we inquired the brief history and techniques of breeding and the current status on Cymbidium breeding in Korea. Also, the general propagation process of elite Cymbidium lines via tissue culture should be presented. However, the slow process of conventional breeding and the lack of useful genes in Cymbidium species delays the introduction of new cultivars to the commercial market. To solve these limitations, efficient regeneration and genetic transformation systems should be established in the improvement of Cymbidium breeding program. During the last several decades, some progress has been made in tissue culture and genetic transformation in Cymbidium species. We review the recent status of tissue culture and genetic transformation systems in Cymbidium plants.

• 한국자원식물학회지
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• 제35권4호
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• pp.508-514
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• 2022

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 mg/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 mg/L BAP and 1.0 mg/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

• Journal of Plant Biology
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• 제22권1_2호
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• pp.35-43
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• 1979

The present investigation has been made to study the deficiency symptoms of boron on the formation of cell wall and the development of the individual components of the orchid cell wall. Analytical samples were taken from two sources; one from the individual orchid plants started from an apical meristem culture followed by the generation of the protocorm-like body which was developed into a plant, the other from the plant cultivated in water for 30 days. The amount of boron in the cultrues were controlled and the deficiency symptoms were observed under theelectron microscope, optical microscope with samples taken from the zones of elongation of leaves and compared the dry weight of cell walls and finally the various fractions of the cell wall components. The following results were obtained: (1) The growth of roots and leaves was hampered in the boron deficient plants. (2) In the boron-deficient leaves a severe necrosis and cracks were developed in the tissue of zone of elongation besides the decrease in growth. (3) under the electorn microscope the cell walls of boron-deficient plants showed rough undulated structures unlike the smooth control cell walls. (4) the dry weight of total cells and cell walls of boron deficient plants were higher than the control plants. (5) In the boron deficient plant the amout of pectin and hemicellulose isolated from cell walls were higher and the amount of protein was lower than the controlled plots.

• 문화기술의 융합
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• 제8권5호
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• pp.235-240
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• 2022

동강 조수호(1924~2016, 이하 '동강')는 최근세에 활동하였던 서예가이면서 문인화가였다. 그는 생존시 세계서단의 일대(一代) 종사(宗師)라고 칭하여진 인물이다. 그가 가지는 미(美)에 대한 인식은 인간의 본능에 의하여 체화된 예술관에 기인하고 있다. 특히 그는 서화를 접(接)의 예술로 보고 있다. 따라서 접의 의미를 '사귀다', '교제하다'와 같은 남녀의 키스, 운우(雲雨)의 정(情)으로 인식하고 있다. 이와 같이 그의 문인화는 예술에 대한 폭넓은 고민과 탐구에서 비롯되었다. 그는 미(美)에 대하여 보편적이고 일반적인 관점에서 예술창조의 원리를 인식하였다. 이를 바탕으로 진정한 예술정신과 예술철학의 의의를 정립하였다. 묵죽과 묵난으로 동강의 문인화의 단면을 살펴보면 그의 묵죽은 무윤(無潤)과 고삽(苦澁)의 심미성이 있다. 또한 묵난은 아(雅)와 운(韻)이 속(俗)에 거(居)하는 것으로 심미된다. 이같이 그의 인품과 학문은 그의 문인화가 되었다.

• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2003년도 추계총회 및 연구발표회 초록집
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• pp.131.1-131
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• 2003

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.330-336
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• 2020

Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.138-145
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• 2016

To find the optimal propagation condition for endangered Cypripedium macranthos Sw., also known as lady's slipper orchid, the effect of various organic additives on in vitro germination, protocorm formation and seedling growth was investigated during asymbiotic seed culture. When $100ml{\cdot}L^{-1}$ coconut water was added to the basal medium, the highest germination rate and protocorm formation rate were achieved, with 70.8% and 74.2% respectively. Supplementation of phloem sap from birch tree or maple tree also showed a facilitating effect to improve the germination and protocorm development. With $100ml{\cdot}L^{-1}$ birch sap or maple sap, both the germination and protocorm formation rates were roughly more than 65% and 68%. The roots and buds of the seedlings grew vigorously in the medium containing $100ml{\cdot}L^{-1}$ coconut water or phloem sap, in particular, their bud formation rates increased by more than 70%. Addition of banana powder and peptone could not create a more significantly favorable culture condition, and non-addition had the worst results. Our results demonstrated that proper organic amendments such as coconut water and phloem sap might be preferred to in vitro germination and the growth of seedlings developed from the protocorm of C. macranthos Sw. during asymbiotic seed culture.