• Journal of Plant Biology
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• v.22 no.1_2
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• pp.35-43
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• 1979

The present investigation has been made to study the deficiency symptoms of boron on the formation of cell wall and the development of the individual components of the orchid cell wall. Analytical samples were taken from two sources; one from the individual orchid plants started from an apical meristem culture followed by the generation of the protocorm-like body which was developed into a plant, the other from the plant cultivated in water for 30 days. The amount of boron in the cultrues were controlled and the deficiency symptoms were observed under theelectron microscope, optical microscope with samples taken from the zones of elongation of leaves and compared the dry weight of cell walls and finally the various fractions of the cell wall components. The following results were obtained: (1) The growth of roots and leaves was hampered in the boron deficient plants. (2) In the boron-deficient leaves a severe necrosis and cracks were developed in the tissue of zone of elongation besides the decrease in growth. (3) under the electorn microscope the cell walls of boron-deficient plants showed rough undulated structures unlike the smooth control cell walls. (4) the dry weight of total cells and cell walls of boron deficient plants were higher than the control plants. (5) In the boron deficient plant the amout of pectin and hemicellulose isolated from cell walls were higher and the amount of protein was lower than the controlled plots.

• Korean Journal of Plant Taxonomy
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• v.49 no.1
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• pp.96-99
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• 2019

The species diversity of Orchidaceae is believed to be extremely high in Myanmar. Like in other plant groups, little basic inventory work has been done thus far. During floristic surveys conducted in Popa Mountain Park (Mandalay Region), Natma Taung National Park (Chin State), and in the Ywangan area (southwestern Shan State), we encountered two interesting orchid species which were determined to be new generic records in the flora of Myanmar. Thuniopsis cleistogama was previously considered endemic to Yunnan Province (China) and was only known from the type locality. However, here the species is newly reported from two localities in central and western Myanmar. Disperis neilgherrensis is known from several widely scattered localities in tropical and subtropical Asia. This tiny orchid species was discovered in the Ywangan area in the southwestern area of the Shan State of Myanmar. These two species will be valuable additions to the updated checklist of the orchids of Myanmar.

• Mycobiology
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• v.50 no.2
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• pp.142-149
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• 2022

Orchids live with mycorrhizal fungi in mutualism. This symbiotic relationship plays an essential role in the overall life cycle of orchids from germination, growth, settlement, and reproduction. Among the 1000 species of the orchid, the Korean lady's slipper, Cypripedium japonicum, is known as an endangered species. Currently, only five natural habitats of the Korean lady's slipper remain in South Korea, and the population of Korean lady's slipper in their natural habitat is not increasing. To prevent extinction, this study was designed to understand the fungal community interacting in the rhizosphere of the Korean lady's slipper living in the native and artificial habitats. In-depth analyses were performed to discover the vital mycorrhizal fungi contributing to habitat expansion and cultivation of the endangered orchid species. Our results suggested that Lycoperdon nigrescens contributed most to the increase in natural habitats and Russula violeipes as a characteristic of successful cultivation. And the fungi that helped L. nigrescens and R. violeipes to fit into the rhizosphere community in Korean lady's slipper native place were Paraboeremia selaginellae and Metarhizium anisopliae, respectively. The findings will contribute to restoring and maintaining the endangered orchid population in natural habitats.

• Journal of Plant Biotechnology
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• v.50
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• pp.108-114
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• 2023

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative Mixture^TM (PPM^TM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPM^TM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPM^TM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPM^TM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPM^TM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.04a
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• pp.187-190
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• 1999

This study is intended to present the properites of planting concrete using the light-weight aggregate and crushed aggregate. According to the result of experiment, the concrete using orchid stone shows a better performance in voids volume, unit weight, absorbtion, thermal conductivity and pH, which are helpful for planting and heat isolation. Therefore, if planting concrete used with orchid stone are utilized to be the planting base on the building's roof, it will be effective to reduced the cost of construction and heating due to the redution of building's self weight and heat isolation effection.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2008.11a
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• pp.193-197
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• 2008

This study enforced to produce the planting concrete block which could be applied to various slopes economically. First of all, the physical properties was investigated with the various types of aggregate and aggregate ratio of the paste for the lead to mixture proportion of the planting concrete. As a result, the orchid stone as aggregate and 30% of aggregate ratio of the paste were used as the basic mixture proportion considering 20~30% of maintained void ratio for the growth of plant, over 20% of capillary suction for holding water, and 3MPa as the minimum strength. For the result of the test to the new planting block which was quite different from existing planting concrete block, it could complement the problems and be possible to produce effectively and economically because various slopes like 40ﾟ~75ﾟ, continual produce by extrusion, and pumping out were possible were possible.

• Journal of Plant Biology
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• v.33 no.4
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• pp.271-276
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• 1990

Our study was carried out for plant regeneration via somatic embryogenesis from immature seeds of Bletilla striata. The highest frequency of embryogenic callus formation was obtained from the immature seeds (at 150 days after pollination) cultured on Hyponex and VW medium supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin under the dark condition. Multiple somatic embryos were induced when embryogenic callus was transferred to VW medium without growth regulators under continued illumination. Somatic embryos were observed histologically with scanning electron microscopy. Regeneration of Bletilla striata was obtained from somatic embryos with a well-defined scutellum and coleoptile as well as with one or more shoot primordia and root primordia. We think that these methods for orchid multiplication must be useful to access clonal propagation of orchids.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.559-562
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• 1998

Odontoglossum ringspot virus (ORSV) was finally purified from ORSV-infected orchid plants by diethylaminoethyl (DEAE) cellulose anion exchange column chromatography. The virus was reliably eluted by potassium chloride at the concentration from 0.1 M to 0.13 M. Partial purification was done by solubilization with Triton X-100 (allkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG; MW 8,000). The finally purified ORSV represented one distinct homogeneous band and the molecular weight of its capsid protein was about 17,500 Dalton in electrophoretic analysis. Electron microscopy showed not only intact particles ranged from 280 nm to 340 nm in length, but also segmented particles that final 140 nm to 220 nm and even disks. Enzyme-linked immunosorbent assay (ELISA) showed that final yield was 12 mg/100 g of the infected leaves. Bioassay demonstrated that the purified ORSV had the normal infectivity to orchid plants and Nicotiana glutionsa. Based on these data, anion exchange column chromatography could be efficiently applied to the purification of ORSV and other viruses similar to ORSV.

• KSBB Journal
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• v.6 no.4
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• pp.385-388
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• 1991

Embryogenic cell suspension cultures of B. striata were established as transferred selected embryogenic callus into liquid medium. Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable transformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200-300 voltage/1180 uF. Protoplast viability was up to the 80% at the optimal voltage.

• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.54-57
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• 2004

These studies were carried out to develop native Calanthe in Korea. Calanthe native to southern islands in Korea has beautiful flowers with various color and sweet fragrance, and it has been reported to have very good ornamental value. According to the morphological characteristics of leaves and flower color, 57 horticultural lines of Calanthe collected in natural crossing group were investigated. And the selected orchid was given a name of horticultural cultivar to ‘Narai’. In its color of flower, sepal was deep purplish red, petal was yellow and lip was white. And also, ‘Narai’ had strong fragrance and shape of blooming was flat-blooming type.