• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6491-6499
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• 2015

Background: Red-fleshed sweet orange juice (ROJ) comes from a new variety of citrus cultivated in Brazil that contains high levels of ${\beta}$-carotene and lycopene, and similar amounts of hesperidin (HSP) and nutrients, equivalently to blond orange juice (BOJ). Such bioactive compounds are associated with chemopreventive actions in several cancer cell lines. The purpose of this study was to examine the cytotoxicity, cell cycle, apoptosis, and cytokine secretion after BOJ, ROJ, and HSP treatment of a novel T acute lymphoblastic leukemia cell line, Loucy. Materials and Methods: Loucy cells were incubated for 24-h with BOJ, ROJ, and HSP, and the viability was measured using trypan blue. Cell cycling and apoptosis were assessed by propidium iodide (PI) and annexin V-FITC/PI flow cytometry, respectively. Secretion of cytokines $IL-1{\alpha}$, $IL1-{\beta}$, IL-2, IL-4, IL-6, IL-10, IL-17A, $IFN{\gamma}$, $TNF{\alpha}$, $TGF{\beta}$, $MIP{\alpha}$, and $MIP{\beta}$ was determined by ELISA array. Results: BOJ and ROJ treatments promoted Loucy cell cytotoxicity. Additionally, BOJ induced cell cycle arrest in the G0/G1 phase, and decreased the cell accumulation in the G2/M. ROJ decreased only the G0/G1 fraction, while HSP did not change the cell cycle. BOJ led to apoptosis in a different fashion of ROJ, while the first treatment induced apoptosis by increase of late apoptosis and primary necrotic fractions, the second increased early and late apoptosis, and primary necrotic fraction compared to positive controls. HSP had no effect on apoptosis. IL-6 and IL-10 were abrogated by all treatments. Conclusions: Taking together, these results suggest potential chemopreventive effects of BOJ and ROJ on Loucy cells.

• 대한화학회지
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• 제22권5호
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• pp.311-316
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• 1978

Fe(III)과 Xylenol Orange (XO 또는 $H_6A$)의 반응을 분광광도법으로 연구하였다. Fe(III)과 XO는 조성이 Fe(III) : XO = 2 : 1 및 1 : 1 인 두가지 착물을 형성함을 확인하였다. 2 : 1 착물은 XO보다 Fe(III)를 많이 포함한 산성에서 안정하고, 1 : 1 착물은 XO가 많이 포함된 약산성용액에서 안정하다. 흡수극대는 590nm(2 : 1)와 500nm(1 : 1)이고 몰흡광계수는 각각 $3.18{\pm}0.04{\times}10^4,\;1.32{\pm}0.03{\times}10^4$이다. pH값을 변화시키면서 측정한 두 착물의 안정도상수는 $log{\beta}_{21}=18.69{\pm}0.03,\;log{\beta}_{212}=42.08{\pm}0.09,\;log{\beta}_{11}=4.17{\pm}0.04,\;and\;log{\beta}_{113}=34.47{\pm}0.07$이었다.

• 한국환경과학회지
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• 제25권12호
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• pp.1717-1726
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• 2016

In this study, the reductive decolorization of three acid and basic dyes using modified zero-valent iron (i.e., acid-washed iron (Aw/Fe) and palladium coated iron (Pd/Fe)) at various pH conditions (pH 3~5) was experimentally investigated and the decolorization characteristics were evaluated by analyzing the absorbance spectra and reaction kinetics. In the case of acid dyes such as methyl orange and eriochrome black T, color removal efficiencies increased as initial pH of the dye solution decreased. However, the color removal of methylene blue, a basic dye, was not affected much by the initial pH and more than 70% of color was removed within 10 min. During the decolorization reaction, the absorbance of methyl orange (${\lambda}_{max}=464nm$) and eriochrome black T (${\lambda}_{max}=528nm$) decreased in the visible range but increased in the UV range. The absorbance of methylene blue (${\lambda}_{max}=664nm$) also decreased gradually in the visible range. Pseudo-zero order, pseudo-first order, and pseudo-second order kinetic models were used to analyze the reaction kinetics. The pseudo-second order kinetic model was found to be the best with good correlation. The decolorization reaction rate constants ($k_2$) of methylene blue were relatively higher than those of methyl orange and eriochrome black T. The reaction rate constants of methyl orange and eriochrome black T increased with a decrease in the initial pH.

• Journal of the Korean Wood Science and Technology
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• 제41권6호
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• pp.528-534
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• 2013

본 연구는 톱밥과 귤박을 이용하여 혼합보드를 제조하여 기본 물성을 조사하였다. 혼합보드의 귤박 혼합율이 10%에서 40%로 증가함에 따라 흡수율은 94.1%에서 86.5%로, 두께 팽창율은 27.2%에서 18.0%로, 휨강도는 $65.1kgf/cm^2$에서 $39.2kgf/cm^2$로, 경도는 $195.3kgf/cm^2$에서 $180.3kgf/cm^2$로 감소하였다. 혼합보드의 밀도가 $0.4g/cm^3$에서 $0.8g/cm^3$로 증가함에 따라 흡수율은 149.2%에서 58.6%로 감소하였으나, 두께 팽창율은 6.4%에서 17.9%로, 휨강도는 $4.2kgf/cm^2$에서 $96.6kgf/cm^2$로, 경도는 $40.4kgf/cm^2$에서 $196.2kgf/cm^2$로 증가하였다.

• Journal of Applied Biological Chemistry
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• 제54권3호
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• pp.214-217
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• 2011

바나나, 오렌지 및 파인애플을 대상으로 시안화수소 처리후 잔류 특성을 평가하여 과실류에 대한 메틸브로마이드 대체 훈증제 개발 가능성을 조사하였다. 메틸브로마이드 훈증소독시 3일차에서 잔류량이 오렌지와 바나나에서는 1 mg/kg 미만 수준으로 나타났고, 파인애플의 경우에도 1 mg/kg을 조금 상회하는 수준으로 잔류허용 기준치 50 mg/kg을 훨씬 하회하고 있어 메틸브로마이드 잔류로 인한 독성문제는 우려할 수준이 아닌 것으로 평가되었다. 시안화수소 훈증소독의 경우 3일차에서 오렌지 50-60%, 바나나 70-80% 그리고 파인애플 50% 정도 분해 소실된 것으로 나타나, 잔류허용 기준치 5 mg/kg을 하회하는 1 mg/kg 미만 수준으로 나타났다. 이는 향후 메틸브로마이드 대체 훈증제로서 시안화수소의 사용이 가능함을 뒷받침하는 유용한 결과로 판단된다.

• Journal of Preventive Medicine and Public Health
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• 제36권2호
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• pp.171-178
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• 2003

Objective : The aim of this study was to identify the validity of recruiting medical checkup participants of Vietnam veterans using a mail survey, and to identify the 'Vietnam service related characteristics' and 'general characteristics' of Vietnam veterans groups, Method : In this study, a total 900 veterans were randomly selected from the list of Vietnam veterans from f964 to 1973. The veterans were classified into 5 groups, taking into consideration their registered status in the Ministry of Patriots and Veterans Affairs: the VRD (veterans who have agent orange-related diseases), VSD (veterans who have agent orange-suspected disease), VM (veterans who peformed meritorious deeds during the war), VR (veterans who were registered with the Ministry of Patriots and Veterans Affair) and OV (other veterans) groups. By means of postal surveys, the veterans' intention to participate in the medical checkup for our research, and their socioeconomic and general characteristics were investigated. 52 surveys were returned due to the subjects not residing at the listed address, and were excluded from the analysis. Result : 699 of the 848 veterans (82.4%) responded to the survey, of which 619 (88.6%) intended to participate in the medical checkup for our research. The S veterans groups all had similar ages, Vietnam service period, Agent Orange exposure, troop characteristic and wartime class, with the exception of VM, who were older, and with a greater number of officers than the other 4 groups, There was a big difference in the health statius among the Vietnam veterans group. The VM and OV were much healthier than VRD, VSD and VR groups, The socioeconomic stati of the VRD, VSD and VR groups were lower than those of the VM and OV groups. Conclusion : Although there were some limitations, the recruitment, by mail, of medical checkup participants from Vietnam veterans is a valid and feasible method. The VM and OV groups were much healthier, and with higher socioeconomic stati, than the VRD, VSD, and VR groups.

• 한국식품조리과학회지
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• 제17권4호
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• pp.373-379
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• 2001

In an attempt to introduce paprika in wet noodles for improved organoleptic and other quality characteristics, the juices of orange paprika was added as a raw ingredient for wet noodle at 0(control), 15, 30, 45, and 60%(v/w). Overall, the cooked wet noodles containing 15 to 60% paprika juices showed higher sensory scores than control(noodle without Paprika juice), and the acceptabilities for color and overall preference of the products were significantly(p＜0.01) higher at the addition levels of 45% and 30 and 45%, respectively. The intensity analysis for the organoleptic quality characteristics of wet noodles with orange paprika juice showed that as the addition levels increased, there were significant(p＜0.001) increase in the values of acceptances for color and flavor of the products, but not in the tenderness and chewiness. Other quality characteristics like water absorption ratio, weight, volume, and turbidity of the broth after cooking of wet noodles increased in values as affected by the addition of paprika juices. In the Hunter's colorimetric characteristics of the products, the decreased lightness(“L”) along with the increased redness(“a”), yellowness(“b”), and ΔE were caused as the amounts of orange paprika juice increased in wet noodles. For toughness, no significant differences(p＜0.001) were noticed among the samples as measured by Waner-Bratzler blade attached to Texture Analyzer. The results of this study suggested that adding orange paprika juice to wet noodles, most preferably at 45%, increases the acceptance and organoleptic qualities of noodle by affecting the color and flavor of the final products.

• Advances in environmental research
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• 제5권3호
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• pp.189-200
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• 2016

This study aimed at advanced oxidation of hetero tri-functional reactive dye Acid orange 7 using photo-Fenton conditions in a lab-scale experiment. Decolourisation of Acid Orange 7 dye by Fenton's process was dependent on concentration of Hydrogen peroxide, Ferrous sulphate, pH, and contact time. A $2^3$ factorial design was used to evaluate the effects of these key factors: pH, Fe(II), and $H_2O_2$ concentration, for a dye concentration of 50 mg/L with COD of 340 mg/L at pH 3.0. The response function was removal of colour under optimised conditions; pH 3.0, [Fe(II)] 40.83 mg/L, [$H_2O_2$] 4.97 mmol/L; 13.6 min. of treatment resulting in 100% colour removal. The final COD of treated wastewater was nil suggesting that AOP is a potentially useful process of color removal and dye degradation/mineralisation of effluent having AO7. Minimum contact time for complete decolourisation was at 5 mmol/l $H_2O_2$ concentration. Increase in $FeSO_4$ (mg/l) concentration resulted in decrease of time for complete decolourisation. Box-Behnken Design was used to optimize the process variables. Maximum and minimum levels of pH (3-5), $H_2O_2$ (4-6 mmol/l), $FeSO_4$ (30-46 mg/l) and contact time (5-15 minutes) were used. The statistical analysis revealed a value of 0.88 for coefficient of regression ($R^2$) indicating a good fit of model. Calculated F-value was found higher than the tabulated value confirming to significance of the model. Based on student's t-test, Ferrous sulphate, pH, and contact time have a positive effect on the percent decolourisation of Acid Orange 7.

• Journal of Periodontal and Implant Science
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• 제28권3호
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• pp.453-465
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• 1998

감초산이 인체 치은 섬유모세포에 미치는 영향을 세포의 성장과 증식, 총 교원질 합성 및 인체 치은 섬유모세포 핵내 acridine orange 결합으로 추적조사하였다. 조절이 되지 않는 성장을 해결하기 위하여 세포분화인자인 감초산이 배양 치은 섬유모세포의 활성에 미치는 효과를 검색하였다. 감초산 존재하의 배양 인체 섬유모세포의 세포성장 및 증식, 교원질 합성 및 세포 핵내 acridine orange 결합을 각각 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)법, 4-hydroxyproline, 유식세포분석기를 이용한 acridine orange 결합으로 검색하였다. 형태학적으로 $100\;{\mu}g/ml$의 감초산으로 처리한 섬유모세포는 모양이 둥글게 되었다. 감초산은 $50\;{\mu}g/ml$ 이상의 농도에서 치은 섬유모세포의 성장과 증식을 억제하였다. 감초산 존재 시에 세포내 총 교원질 양이 감소하였고, 세포외배지내의 교원질 총 양이 증가하였다. 인체 치은 섬유모 세포를 $100\;{\mu}g/ml$의 감초산과 함께 24 시간동안 배양하였을 때, 80 채널 이상의 평균형광을 갖는 diploid 세포가 감소하였고, 80 채널 이하의 형광을 갖는 acridine orange결합이 증가하였다. 이러한 연구 결과 감초산은 인체 섬유모세포에서 세포성장 및 증식, 교원질합성 및 DNA 분절화를 유도함이 제시하였다.

• Preventive Nutrition and Food Science
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• 제19권4호
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• pp.291-298
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• 2014

The aim of this study was to investigate the antioxidant activity of orange (Citrus auranthium) flesh (OF) and peel (OP) extracted with acetone, ethanol, and methanol. Antioxidant potential was examined by measuring total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA), total radical-trapping antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), and cellular antioxidant activity (CAA). The comet assay was used to determine the protective effects of OF and OP against $H_2O_2$-induced DNA damage. TPC was highest in the acetone extracts of OF and OP. DPPH RSA was also higher in the acetone extracts than in the ethanol extracts. The DPPH RSA was highest in the acetone extracts of OF. The TRAP and ORAC values of the all extracts increased in a dose-dependent manner. In the TRAP assay, the acetone extracts of OF and OP had the lowest $IC_{50}$ values. In the CAA assay, the methanol and acetone extracts of OP had the lowest $IC_{50}$ values. All of the samples protected against $H_2O_2$-induced DNA damage in human leukocytes, as measured by the comet assay, but the acetone extracts of OP had the strongest effect. These results suggest that acetone is the best solvent for the extraction of antioxidant compounds from OF and OP. Furthermore, the high antioxidant activity of OP, which is a by-product of orange processing, suggests that it can be used in nutraceutical and functional foods.