• Biomolecules & Therapeutics
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• v.4 no.2
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• pp.184-189
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• 1996

General pharmacological studies of LB20304a (a mesylate salt form of a new quinolone antibiotic LB20304 following oral administration of 300 mg/kg and 1000 mg/kg, almost maximum tolerance dose in mice and rat, respectively, were performed in terms of effects on general behaviour, central nervous system, gastrointestinal system, and blood coagulation system in mice and rats. With regards to general behaviour of mice, at oral dose of 300 mg/kg, LB20304a reduced muscle tone and locomotor activity. In terms of CNS, at oral treatment of 300 mg/kg, LB20304a showed some analgesic effects in mice, and oral dose of 1000 mg/kg caused drop in normal body temperature of rat, while it enhanced the pentylenetetrazole-induced clonic convulsion to tonic convulsion and/or death in mice at the doses of unto 300 mg/kg. In addition, LB20304a increased hexobarbital-induced sleeping time two and three times in mice at oral doses of 20 mg/kg and 300 mg/kg, respectively. Rota-rod and traction test in mice were not influenced by the dose of 300 mg/kg and 200 mg/kg, respectively. LB20304a reduced gastric secretion of rat at dose of 1000 mg/kg, and increased intestinal motility of mice at dose of 300 mg/kg. In rats, blood coagulation index, such as PT (prothrombin time) and aPTT (activated partial thromboplastin time) were not affected by the treatment of upto 1000 mg/kg of LB 20304a.

• Journal of Oral Medicine and Pain
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• v.30 no.4
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• pp.457-464
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• 2005

The purpose of our study was to investigate whether the intrapulpal temperature during cavity preparation of enamel or dentin with Er:YAG laser still remained in range of safety for dental pulp protection when combined with appropriate water flow rate. The effect of different pulse repetition rates at the same pulse energy during ablation was evaluated as well. Caries-free, restoration-free extracted human molar teeth were prepared for the specimen and divided two experimental groups of enamel and dentin. Each group comprised 5 specimens and each of tooth specimens were embedded into a resin block each and measuring probe was placed on the irradiated pulpal walls. For experiments of dentin ablation, enamel layers were prepared to produce dentin specimen with a same dentin thickness of 2 mm. A pulse energy of Er:YAG laser was set to 300 mJ and three different pulse repetition rates of 20 Hz, 15 Hz and 10 Hz were employed. Laser beam was delivered with 3 seconds and less per application over enamel and dentin surfaces constant sized by $3\;mm{\times}2\;mm$ and water spray added during irradiation was a rate of 1.6 ml/min. Temperature change induced by Er:YAG laser irradiation was monitored and recorded While enamel was ablated, there was no significant difference of temperature related to pulse repetition rates(p=0.358) and temperature change at any pulse repetition rate was negligible. Significant statistical difference in temperature changes during cavity preparation in dentin existed among three different pulse groups(p=0.001). While temperature rise was noticeable when the dentinal wall was perforated, actual change of temperature due to Er:YAG laser irradiation was not enough to compromise safety of dental pulp when irradiation was conjugated with appropriate water spray. Conclusively, it can be said that cavity preparation on enamel or dentin with an Er:YAG laser is performed safely without pulp damage if appropriate volume of water is sprayed properly over the irradiated site.

• The Journal of the Korean dental association
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• v.22 no.5 s.180
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• pp.409-416
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• 1984

• Maxillofacial Plastic and Reconstructive Surgery
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• v.32 no.1
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• pp.9-15
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• 2010

Aim of the study: As an injectable scaffold, MPEG-PCL diblock copolymer was applied in bone tissue engineering. In vivo bone formation was evaluated by soft X-ray, histology based on the rat calvarial critical size defect model. Materials and Methods: New bone formation was evaluated with MPEG-PCL diblock copolymer in rat calvarial critical size bone defect. No graft was served as control. 4, 8 weeks after implantation, gross evidence of bone regeneration was evaluated by histology and soft X-ray analysis. Results: The improved and effective bone regeneration was achieved with the BMP-2 and osteoblasts loaded MPEG-PCL diblock copolymer. Conclusion: It was confirmed that MPEG-PCL temperature sensitive hydrogels was useful as an injectable scaffold in bone regeneration.

• Journal of Oral Medicine and Pain
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• v.22 no.1
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• pp.15-22
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• 1997

In order to investigate the antibacterial effects of pulsed Nd:YAG Laser concerning to Streptococcus mutans, Streptococcus mutans was cultured and standardized and plated in 96 well plates. After that, black dye and chlorhexidine were applied and pulsed Nd:YAG Laser was irradiated to plates. Experimental groups which was applied with black dye and chlorhexidine were divided into 5 groups by concentration. And experimental groups and control group were subdivided as unlased and lased of 20, 40, and 60J. After laser irradiation, All groups were plated in agar plates and incubated for 18 hours. Bacterial colonies were counted and analysed comparitively, and the temperature of culture medium was measured on every laser irradiation. The results were obtained as follows; 1. In case of laser irradiation only, there is no significant reduction in number of Streptococcus mutans by irradiated energy of laser. 2. In case of black dye application, there is no difference by concentration of black dye but there is decrease when applied Laser and black dye simultaneously. 3. When applied chlorhexidine, the number of Streptococcus mutans was decreased according to increase of concentration, but there is no difference by irradiated energy of laser. 4. The temperature of culture medium was increased according to increase of irradiated energy of Laser, and the number of Streptococcus mutans was reduced remarkably just above 50 centigrade and killed above 70 centigrade. As above results, pulsed Nd:YAG Laser has well-absorbing affinity to black dye. And antibacterial effect of pulsed Nd:YAG Laser against Streptococcus mutans is seemed to be due to increase of temperature.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.6
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• pp.603-611
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• 2021

Taste-responsive neurons in the nucleus of the solitary tract (NST), the first gustatory nucleus, often respond to thermal or mechanical stimulation. Alcohol, not a typical taste modality, is a rewarding stimulus. In this study, we aimed to investigate the effects of ethanol (EtOH) and/or temperature as stimuli to the tongue on the activity of taste-responsive neurons in hamster NST. In the first set of experiments, we recorded the activity of 113 gustatory NST neurons in urethane-anesthetized hamsters and evaluated responses to four basic taste stimuli, 25% EtOH, and 40℃ and 4℃ distilled water (dH₂O). Sixty cells responded to 25% EtOH, with most of them also being sucrose sensitive. The response to 25% EtOH was significantly correlated with the sucrose-evoked response. A significant correlation was also observed between sucrose- and 40℃ dH₂O- and between 25% EtOH- and 40℃ dH₂O-evoked firings. In a subset of the cells, we evaluated neuronal activities in response to a series of EtOH concentrations, alone and in combination with 32 mM sucrose (EtOH/Suc) at room temperature (RT, 22℃-23℃), 40℃, and 4℃. Neuronal responses to EtOH at RT and 40℃ increased as the concentrations increased. The firing rates to EtOH/Suc were greater than those to EtOH or sucrose alone. The responses were enhanced when solutions were applied at 40℃ but diminished at 4℃. In summary, EtOH activates most sucrose-responsive NST gustatory cells, and the concomitant presence of sucrose or warm temperatures enhance this response. Our findings may contribute to elucidate the neural mechanisms underlying appetitive alcohol consumption.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.47 no.3
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• pp.197-208
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• 2021

Objectives: The purpose of this retrospective study was to evaluate the variables associated with length of stay (LOS), hospital costs, intensive care unit (ICU) use, and treatment outcomes in patients hospitalized for maxillofacial infections at a tertiary medical center in South Korea. Materials and Methods: A retrospective chart review was conducted for patients admitted for treatment of maxillofacial infections at Dankook University Hospital from January 1, 2011 through September 30, 2020. A total of 390 patient charts were reviewed and included in the final statistical analyses. Results: Average LOS and hospital bill per patient of this study was 11.47 days, and ₩4,710,017.25 ($4,216.67), respectively. Of the 390 subjects, 97.3% were discharged routinely following complete recovery, 1.0% expired following treatment, and 0.8% were transferred to another hospital. In multivariate linear regression analyses to determine variables associated with LOS, admission year, infection side, Flynn score, deep neck infection, cardiovascular disease, admission C-reactive protein (CRP) and glucose levels, number and length of surgical interventions, tracheostomy, time elapsed from admission to first surgery, and length of ICU stay accounted for 85.8% of the variation. With regard to the total hospital bill, significantly associated variables were age, type of insurance, Flynn score, number of comorbidities, admission CRP, white blood cell, and glucose levels, admission temperature, peak temperature, surgical intervention, the length, type, and location of surgery, tracheostomy, time elapsed from admission to first surgery, and length of ICU use, which accounted for 90.4% of the variation. Age and ICU use were the only variables significantly associated with unfavorable discharge outcomes in multivariate logistic regression analysis. Conclusion: For successful and cost-effective management of maxillofacial infections, clinicians to be vigilant about the decision to admit patients with maxillofacial infections, perform appropriate surgery at an adequate time, and admit them to the ICU.

• Journal of Periodontal and Implant Science
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• v.37 no.1
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• pp.35-44
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• 2007

Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybrid-ization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22 expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed In the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly ex-pressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the re-results suggest that further studies will be needed to produce the considerable amount of PDLs22 re-combinat protein, which can use for the periodontal regeneration.

• Journal of Korean Academy of Nursing
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• v.4 no.2
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• pp.93-106
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• 1974

The purposes of this study are to identify the necessity of utilization of electric thermometer, to determine the difference of clinical thermometers to reach maximum or optimum temperature, and to determine the length of time necessary for temperature taking, with Fahrenheit thermometer, electric thermometer, Yu Ⅱ centigrade thermometer, and Kuk ll centigrade thermometer. The first and second comparative Experiments were' conducted from August 25 through September 30, 1973. In the first experiment, Fahrenheit thermometer, which had been accurately teated two times, and electric thermometer have been utilized. These two kinds of thermometers were inserted simultaneously under the central area of the tongue and the mouth kept closed while thermometers were in place. All temperature readings were done at one minute interval until leaching-maximum temperature. These procedures were repeated one hundred times and the data were-analyzed statistically by means of the t-test. In the second experiment, Fahrenheit thermometer, which had been accurately tested two. times, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer have been utilized. These three kinds of thermometers were inserted simultaneously under the central area of the. tongue and the mouth kept closed while thermometer were in place. All temperature readings were done at one minute interval until reaching maximum temperature. These procedures were. repeated one hundred times and the data were analyzed statistically by means of the F-ratio Under the eight hypotheses designed for this study, the findings obtained are as follows: 1. There were no significant differences in the maximum temperature between Fahrenheit thermometer and electric thermometer. The mean maximum temperature for Fahrenheit thermometers was 37.06℃ and for electric thermometer was 37.09℃. 2. The placement time to reach maximum temperature taken by Fahrenheit thermometer was significantly shorter than that by electric thermometer. The mean placement time for Fahrenheit thermometers was 4.04 minutes, for electric thermometer was 5.52 minutes. In the case of Fahrenheit thermometers, 45 to 77 percent after 3 to 5 minutes, over 90 Percent after 7 minutes, and 100 percent after 10 minutes, had reached optimum temperature. When the electric thermometer was used, 23 to 54 percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 percent after 12 minutes, had reached optimum temperature. 5. There ware no significant differences in the maximum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer. The mean maximum temperature for Fahrenheit thermometers was 36.67℃, for Yu Ⅱ centigrade thermometer, was 33.73℃, and for Kuk Ⅱ centigrade thermometers was 37.76℃. 6. There were no significant differences in placement time to reach maximum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade Thermometer, and Kuk Ⅱ centigrade thermometer. The mean placement time (or Fahrenheit thermometers was 7.77 minutes, for Yu Ⅱ centigrade thermometers was 7.25 minutes, and Kuk Ⅱ centigrade thermometers was 7.25 minutes. In the case of Fahrenheit thermometers, 8 to 24 percent after 3 to 5 minutes, over 90 percent after 11 minutes, and 100 percent after 13 minutes, had reached maximum temperature. When the Yu Ⅱ centigrade thermometer was used, 10 to 27 percent after 3 to 5 minutes, over 90 percent after 11 minutes, an8 103 percent after 13 minutes, had reached maximum temperature. When the Kuk Ⅱ centigrade thermometer was used, 11 to 27 Percent after 3 to 5 minutes, over 90 percent after 11 minutes, and 100 percent after 12 minutes, had reached maximum temperature. 7. There were no significant differences in the optimum temperature(the maximum temperature minus 0.1℃) among fahrenheit thermometer, Yu Ⅱcentigrade thermometer, and Kuk Ⅱ centigrade thermometer. The mean optimum temperature for Fahrenheit thermometers was 36.60℃, for Yu Ⅱ centigrade thermometers was 36.69℃, and Kuk Ⅱ centigrade thermometers was 36.69℃. 8. There were no significant differences in placement time to reach optimum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer The mean placement time for Fahrenheit thermometers was 5.70 minutes, for Yu Ⅱ centigrade thermometers was 5.54 minutes, and for Kuk Ⅱ centigrade thermometers was 5.28 minutes. In the case of Fahrenheit thermometers, 21 to 49 percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 percent after 12 minutes, had reached optimum temperature. When the Yu Ⅱ centigrade thermometer was used, 23 to 51 percent after 3 to 5 minutes over 90 percent after 10 minutes, and 100 percent after 12 minutes, had reached optimum temperature. When the Kuk Ⅱ centigrade Thermometer was used, 23 to 57 Percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 Precent after 11 minutes, had reached optimum temperature.

• Journal of The Korean Dental Society of Anesthesiology
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• v.5 no.2 s.9
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• pp.112-116
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• 2005

Facial nerve paralysis following delayed complication after trauma was rare and hard to find reason After symptom of facial nerve paralysis was found, careful clinical and neuropathic investigation needed through electromyography and nerve conduction velocity. It is necessery to Hewing that functional degenaration of nerve conduction was irresible or not. It is important to determine if palsy is already present alter trauma or some later time because origin of viral infection or temperature change may possible.