• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제45권6호
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• pp.332-342
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• 2019

Objectives: Socket grafting is vital to prevent bone resorption after tooth extraction. Several techniques to prevent resorption have been described, and various bone graft substitutes have been developed and used with varying success. We conducted this pilot study to evaluate the performance of nanohydroxyapatite (nHA) derived from chicken eggshells in socket preservation. Materials and Methods: This was a prospective, single center, outcome assessor-blinded evaluation of 23 sockets (11 patients) grafted with nHA and covered with platelet-rich fibrin (PRF) membrane as a barrier. Bone width and radiographic bone density were measured using digital radiographs at 1, 12, and 24 weeks post-procedure. Postoperative histomorphometric and micro-computed tomography (CT) evaluation were performed. The study protocol was approved by the Institutional Ethics Committee. Results: All patients had uneventful wound healing without graft material displacement or leaching despite partial exposure of the grafted socket. Tissue re-epithelialized with thick gingival biotype (>3 mm). Width of the bone was maintained and radiographic density increased significantly with a trabecular pattern (73.91% of sockets) within 12 weeks. Histomorphometric analysis showed 56.52% Grade 3 bone formation and micro-CT analysis revealed newly formed bone with interconnecting trabeculae. Conclusion: Use of a PRF membrane with nHA resulted in good bone regeneration in sockets. Use of a PRF membrane prevents periosteal-releasing incisions for primary closure, thereby facilitating the preservation of keratinized mucosa and gingival architecture. This technique, which uses eggshell-derived nHA and PRF membrane from the patient's own blood, is innovative and is free of disease transfer risks. nHA is a promising economic bone graft substitute for bone regeneration and reconstruction because of the abundant availability of eggshell waste as a raw material.

• 치위생과학회지
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• 제23권2호
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• pp.88-92
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• 2023

Background: Aphthous stomatitis is a common disease of the oral mucosa and its pathogenesis is associated with several risk factors. Frequently, minor ulcers are idiopathic in nature and often resolve naturally. However, those ulcers are painful and sensibly compromise patients' eating. There are different treatment strategies for the clinical management of oral aphthae. Methods: The present study assessed the efficacy of a film-forming cream in accelerating the healing and diminishing the pain associated with minor aphthae in a randomized fashion design. The test product (AphtoFix^®) was compared with placebo cream in a cohort of patients with a diagnosis of minor recurrent stomatitis. Patients were randomly distributed into two groups and were followed for 10 days. The primary outcomes included the number of days until symptoms were relieved and the number of days to complete healing. Results: Thirty-six patients completed the follow-up, eighteen per group. All lesions eventually healed within day 10. However, patients in the test group experienced significantly less pain, already from day 1. Patients in the test group also showed a faster healing rate of the lesion with an average of 7 days against the 9 days required for patients in the control group. Conclusion: The present study supported the utility of a film-forming in cream in relieving the patient from symptoms associated with aphthous stomatitis already at day 1 of product use. Patients also displayed faster healing of the lesions when compared to the control group. Further studies with greater sample size and patient stratification according to age and risk factors are recommended to support the present findings.

• 치위생과학회지
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• 제23권4호
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• pp.369-377
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• 2023

Background: In this study, we investigated the potential use of keratin for oral tissue regeneration. Keratin is well-known for its effectiveness in skin regeneration by promoting keratinization and enhancing the elasticity and activity of fibroblasts. Because of its structural stability, high storability, biocompatibility, and safety in humans, existing research has predominantly focused on its role in skin wound healing. Herein, we propose using keratin proteins as biocompatible materials for dental applications. Methods: To assess the suitability of alpha-keratin protein as a substrate for cell culture, keratin was extracted from human hair via PEGylation. Viabilities of primary human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) were assessed. Fluorescence immunostaining and migration assays were conducted using a fluorescence microscope and confocal laser scanning microscope. Wound healing and migration assays were performed using automated software to analyze the experimental readout and gap closure rate. Results: We confirmed the extraction of alpha-keratin and formation of the PEG-g-keratin complex. Treatment of HGFs with keratin protein at a concentration of 5 mg/ml promoted proliferation and maintained cell viability in the test group compared to the control group. HOKs treated with 5 mg/ml keratin exhibited a slight decrease in cell proliferation and activity after 48 hours compared to the untreated group, followed by an increase after 72 hours. Wound healing and migration assays revealed rapid closure of the area covered by HOKs over time following keratin treatment. Additionally, HOKs exhibited changes in cell morphology and increased the expression of the mesenchymal marker vimentin. Conclusion: Our study demonstrated the potential of hair keratin for soft tissue regeneration, with potential future applications in clinical settings for wound healing.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권2호
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• pp.151-156
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• 2006

술전 경부 임파절 평가의 정확성은 신체검사와 방사선학적 소견 모두에서 정확성의 한계를 보였으며, 정확성을 보다 높이기 위해서는 임상의사와 방사선의사간에 의견 교환이 중요하다고 사료된다. 그리고 필요에 따라서는 초음파 촬영, 세침 흡인세포검사(FNAC) 등의 보조적인 임파절 평가 방법을 이용하는 것도 전체 정진율을 높일 수 있을 것이다. 또한 구강암의 원발 부위가 고위험군인 경우에는 가음성도 비율이 높고, 방사선학적 검사에서 전체 정진율이 낮은 결과를 보이는 것으로 볼때, 지금까지 알려진대로 예방적 경부 곽청술을 보다 적극적으로 고려하는 것이 필요하다고 사료된다.

• 구강회복응용과학지
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• 제36권2호
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• pp.70-79
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• 2020

목적: 상악 임플란트 피개의치에서 유지장치 종류와 구개 피개 유무에 따른 응력분포를 3차원 유한요소분석법을 통해 알아보고자 한다. 연구 재료 및 방법: CAD를 이용하여 전방부에 4개의 임플란트를 식립한 4종류의 상악 임플란트 피개의치에 대한 3차원 모델을 디자인하였다. 1) Ball-F: 볼 유지장치를 사용하고 구개를 피개한 군, 2) Ball-P: 볼 유지장치를 사용하고 구개를 피개하지 않은 군, 3) Bar-F: 바 유지장치를 사용하고 구개를 피개한 군, 4) Bar-P: 바 유지장치를 사용하고 구개를 피개하지 않은 군. 응력분석은 유한요소 분석 프로그램인 ANSYS Workbench Ver. 14를 사용하였으며, 편측 저작의 상황을 고려하여 우측 제1대구치에 100 N의 정적하중을 적용하여 임플란트, 임플란트 주위 골, 점막에 발생하는 최대 응력값을 기록하였다. 결과: 볼 유지장치를 사용한 군이 바 유지장치를 사용한 군보다 임플란트 및 임플란트 주위골에 발생하는 최대 응력값이 작았다. 하지만 점막에서의 최대 응력값은 그 반대로 나타났다. 구개를 피개하면 유지장치의 종류와 무관하게 임플란트, 임플란트 주위 골, 점막 모두에서 최대응력값이 작아졌다. 결론: 본 연구의 한계 내에서 상악동 전방부에 4개의 임플란트를 식립하여 수복된 피개의치에서 바 유지장치보다는 볼 유지장치를 사용하고 구개를 완전히 피개하는 경우가 임플란트와 임플란트 주위 골의 응력분산에 효과적이다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제32권4호
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• pp.324-331
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• 2010

The radial forearm free flap (RFFF) has become a workhorse flap as a means of reconstructing surgical defects in the head and neck region. We have transferred 12 RFFFs with fasciocutaneous type on oral cavity defects in 12 patients after cancer resection and submucous fibrotic lesion ablation from 2005 to 2007 at Department of oral and maxillofacial surgery, Pusan National University Hospital. We reviewed retrospectively patients' charts and followed up the patients. Clinical analysis on the cases with RFFFs focusing on flap morbidity, indications and available vessels was done. The results of study are follows: 1. RFFF could be applied for all kind of defects after resection of tongue, floor of mouth, buccal mucosa, denuded bone of palate, maxilla, and mandible. 2. All free flaps could be used for primary reconstruction. The survival rate of 12 RFFFs was 92%. Partial marginal loss of the flaps was shown as 3 cases among 12 cases. Large size-vessels like superior thyroid artery, facial artery, internal jugular vein were favorable for microvascular anastomosis. 3. Parenteral nutrition instead of nasal L-tube also can be favorable for postoperative a week for better healing of the flap if the patients couldn't be tolerable with nasal tubing. 4. Donor sites with thigh skin graft were repaired with wrist band for 2 weeks. The complications included scarring, abnormal sensation on hand, and reduced grip strength in few patients, but those didn't induce major side effects. 5. Most RFFFs were well healed even if mortality rate of cancer patients was shown as 50% (5/10 persons). The mortality of patients was not correlated with morbidity of the flaps. We could identify the usefulness of RFFF for restoration of oral function, esthetics if the flap design, tissue transfer indications, and well controlled operation are proceeded.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제33권6호
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• pp.660-668
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• 2007

We investigated 248 patients who were diagnosed as malignant tumor in the department of Oral and maxillofacial Surgery of Kyungpook National University from 1999 to 2006, and following results were obtained. 1. Among 248 patients who have malignant tumor, 164 were men and 84 were women, which made the ratio of male to female 1.95:1. 2. The average age of oral cancer patients was 58.3. 3. As of the primary origin site, lower alveolus and gingiva were the greatest with 70 cases(28.2%), followed by tongue(l6.9%), upper alveolus and gingiva(14.9%), palate(13.7%), mouth floor(9.7%), buccal mucosa(4.8%), retromolar trigone(4.4%), Mx. & Mn. bone(3.2%) and lip(2.8%). 4. As of histologic distribution, squamous cell carcinoma was the greatest with 170 cases(68.6%), followed by sarcoma with 17 cases(6.9%), adenoid cystic carcinoma with 17 cases(6.9%), malignant lymphoma with 15 cases(6.0%), mucoepidermoid carcinoma with 13 cases(5.2%), metastatic carcinoma with 6 cases(2.4%) and malignant melanoma with 4 cases(1.6%). 5. Period between recognition of the symptom and the first visit to hospital was less than 3 months for 58.9% of the patients, and more than 3 months for 41% of the patients. 6. Investigation of whether the patients drink or smoke revealed that the number of non-smoking and non-drinking patients was 63 among 170 patients(37.0%) that were able to investigate. The number of patients who smoke only was 29(17.1%) and both drinking and smoking patients were 78(45.9%). 7. In clinical stage order, Stage IV(61.7%) was found th be the largest, followed by stage I(17.2%), stage II(13%) and stage III(7.8%). 8. The 5-year survival rate of the entire oral cancer patients appeared to be 57.7%. The survival rate was higher in younger group and women had higher survival rate but there was no statistical significance to this. In the aspect of stage, the survival rate was Stage I, Stage II, Stage IV and Stage III in decreasing order. The order according to T classification was the same. In N classification, patients with N0 had the highest survival rate and the survival rate decreased in the order of N1 and N2. Survival rate was especially low in patients with N2.

• 대한한방내과학회지
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• 제19권2호
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• pp.185-207
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• 1998

In order to study the immunohistochemical effects of Opae-san on gastric ulcer induced by HCl-aspirin in rats, experiments were done by oral administration and measure histological features of ulcer lesion, scaning electron microscopic appearance, the changes of numbers of parietal cells, chief cells, gastrin and somatostatin-immunoreactive cells. The obtained results are as follows: 1. Ulcerative lesions were numerously detected in control groups especially in junction of cardiac-fundic gastric mucosa and histologically very severe injury to gastric epithelium were observed too but in the Opae-san administrated groups, no gross lesion of ulcer were detected and histologically minor injury of gastric mucosa were observed. Most slight injuries to gastric mucosa were observed in 5 days after treatment. 2. The numbers of parietal cells were remarkably increased in control group but in Opae-san administrated groups appeared significant decrease compared to control groups. Most remarkably decrease of the numbers of parietal cells compared to control groups were observed in 5 days after treatment. 3. The numbers of chief cells were remarkably decreased in control group but in Opae-san administrated groups appeared significant increase compared to control groups. Most remarkably increase of the numbers of chief cells compared to control groups were observed in 5 days after treatment. 4. The numbers of gastrin-immunoreactive cells were remarkably decreased in control group but in Opae-san administrated groups appeared significant increase compared to control groups. Most remarkably decrease of the numbers of gastrin-immunoreactive cells compared to control groups were observed in 5 days after treatment. 5. The numbers of somatostatin-immunoreactive cells were remarkably decreased in control group but in Opae-san administrated groups appeared significant increase compared to control groups. Most remarkably decrease of the numbers of somatostatin-immunoreactive cells compared to control groups were observed in 5 days after treatment. 6. Scaning electron microscopically, severe denude and degeneration of gastric mucosa were observed in control groups but in Opae-san administrated groups the lesions were remarkably decreased compared to control groups.

• Current Research on Agriculture and Life Sciences
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• 제1권
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• pp.201-214
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• 1983

Aspirin이 위점막(胃粘膜)에 손상(損傷)을 일으키는 기전(機轉)을 해명(解明)하고 침해(侵害)받는 세포(細胞)의 종류(種類) 및 형태(形態)를 구명(究明)하고자 rat에 체중(體重) 1kg당(當) 60mg을 경구(經口) 및 피하(皮下)로 투여(投與)한 후(後) 경시적(經時的)으로 임상소견(臨床所見)을 관찰(觀察)함과 동시(同時)에 위점막세포(胃粘膜細胞)의 형태적변화(形態的變化)를 광학(光學) 및 전자현미경적(電子顯微鏡的)으로 관찰(觀察)한 결과(結果)를 요약(要約)하면 다음과 같다. aspirin의 경구(經口) 및 피하투여(皮下投與)에 의(衣)하여 특별(特別)한 임상소견(臨床所見)이나 위점막(胃粘膜)의 육안적(肉眼的) 및 광학현미경적(光學顯微鏡的) 소견(所見)은 인정(認定)되지 않았으나 미세형태학적(微細形態學的)으로는 투여경로(投與經路)에 관계(關係)없이 명백(明白)한 변화(變化)를 인정(認定)할 수 있었다. 즉(卽) 위점막세포중(胃粘膜細胞中) 가장 심하게 손상(損傷)받는 세포(細胞)는 벽세포(壁細胞)와 주세포(主細胞)로서 벽세포(壁細胞)에서는 SER과 intracellular canaliculi의 증식(增殖)이, 그리고 주세포(主細胞)에서는 RER의 단열(斷裂), 확장(擴張) 및 효소원과립(酵素原顆粒)의 수적감소(數的減少)와 구조적이상(構造的異常)과 같은 가역적(可逆的) 변성변화(變性變化)가 인정(認定)되었으며 점액세포(粘液細胞), 점액경세포(粘液頸細胞), 기저과립세포(基底顆粒細胞)는 비교적(比較的) 경(經)한 변화(變化)를 보였다. 경구투여군(經口投與群)보다는 경미(輕微)하지만 피하주입군(皮下注入群)에서도 경구투여군(經口投與群)에 상응(相應)하는 변화(變化)를 나타낸 점(点)은 aspirin에 의한 위점막(胃粘膜) 손상(損傷)이 aspirin의 직접적영향외(直接的影響外)에 혈중(血中) aspirin 농도(濃度)에 의(依)한 간접적손상(間接的損傷)도 가미(加味)된다는 것을 시사(示唆)하는 형태적소견(形態的所見)이라고 생각(生覺)되었다.

• 한국산학기술학회논문지
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• 제15권9호
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• pp.5644-5651
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• 2014

본 연구는 칸디다 알비칸스(C. albicans)에 대한 프로폴리스의 억제효과를 확인하고자 수행되었다. 프로폴리스는 양봉 꿀벌에서 채취하여 C. albicans는 $37^{\circ}C$에서 액체 배지에서 2시간 배양 하였다. 항균 활성검삼를 위해서 생리식염수 (PBS), 3% 차아염소산나트륨 (NaOCl에), 0.1% 클로로헥시딘 (CHX), 프로 폴리스 추출물 ($5{\mu}L/m{\ell}$, $10{\mu}L/m{\ell}$)에서 평가하였다. C. albicans는 3%의 NaOCl, 0.1% CHX, 프로 폴리스($5{\mu}L/m{\ell}$, $10{\mu}{\ell}/m{\ell}$)에서 15, 14.5, 16, 17mm의 억제구간을 확인하였다. 항균활성을 확인하기 위하여 집락형성을 분석한 결과, 3%의 NaOCl, 0.1 % CHX, $5{\mu}L/m{\ell}$와 $10{\mu}L/m{\ell}$의 프로 폴리스가 7, 7, 5, 7 로그지수가 감소를 보였다. C. albicans서는 $10{\mu}L/m{\ell}$의 프로폴리스에서 유의하게 억제되는 결과를 보여주었다. 이러한 결과를 통하여 프로폴리스는 구강 점막 질환에서 새로운 향균 약제가 될수 있는 것으로 생각된다.