• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권2호
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• pp.115-121
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• 1998

Train of chemical and biological processes was investigated to treat leachate from industrial waste landfill. Organics and nitrogen concentrations of landfill leachate studied in this research were high and their BOD/COD ratio was 0.3. Biological process should be combined with chemical process for optimum treatment of leachate. PAC(Polyaluminium chloride) was the best coagulant among three chemicals tested, and the optimum condition of PAC coagulation was pH 6 and 1,250 mg/L of dosage. When SBR was operated for simultaneous removal of organics and nitrogen, removal efficiency of COD and T-N reached over $82\%,\;71\%,$ respectively and time distribution of 2/4/2/1 was most effective for one cycle of anoxic/aerobic/anoxic/aerobic.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 춘계학술대회 논문집
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• pp.740-744
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• 2011

Precast slab track system(PSTS) is a concrete track laying system where the slab panels are pre-manufactured in factories and assembled and installed on-site. PSTS has been developed for the past 30 years in countries where railway technologies are advanced such as Japan and Germany to improve the various drawbacks of the in-situ concrete slab track. However, the usefulness of PSTS is being continuously approved by many other countries such as China, Taiwan, Austria, Italy, Spain, etc,. Lately, not only Japan and Germany, but also Austria, Italy and China have developed their own PSTS by collaboration between their Governments and private enterprises and are now attempting to expand their businesse soverseas. In accordance to such movement, in 2006, the Korean Railroad Research Institution and Sampyo E&C have developed a Korean PSTS by joint research. PSTS consists of concrete panel, under pouring layer and concrete base layer. Amongst these components, the panel is the main component of PSTS which supports the train load and has a great effect on the track quality, workability and economics. Therefore, a study is to be conducted to select the optimum size for the Panel of the precast slab track system panel by analyzing the various standards & forms, interpretation of finite elements of the selected model and economical analysis.

• 대한환경공학회지
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• 제28권9호
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• pp.905-910
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• 2006

본 연구는 강원도의 환경기초시설 설치에 따른 주민지원금의 현황을 파악하고 처리형태에 따른 합리적인 주민지원금을 산정하기 위하여 수행되었다. 강원도에 지원된 주민지원금은 환경기초시설 설치비용에 $1.6{\sim}75.0%$로 과도하게 지원된 것으로 조사되었다. 합리적 주민지원금 산정방안은 평균 인구분포, 주민지원금 지원현황, 법적기준 및 전문가 설문조사 결과를 적용하여 수행하였다. 본 연구에서 산정한 처리형태별 주민지원금 범위는 폐기물 처리시설은 설치비용의 20% 범위, 음식물류 폐기물처리시설은 10% 범위, 하수 분뇨합병처리시설은 $3{\sim}5%$ 범위로 산정되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.445-449
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• 2003

As for the purpose, we first introduce an random mutation into wild-type gene to expand a mutation space, and then further recombine the mutant genes by staggered extension process PCR. As a result, we obtained the best clones 6-52 that showed a high activity and stability, from a round of error prone and staggered extension process PCR. The purified enzyme showed a similar pH stability to the wild-type enzyme and reveal a slightly high optimum pH at 12. In the optimum temperature, an identical dependency was also showed and a quite high stability in the thermal stability was obtained. Along with this, the enzyme was also stable at a reaction that supplement with a 15 % of ethanol as an additive. The addition of other solvents and surfactants did not improve the reaction and thus resulted in a similar profile to those of wild-type enzyme. The specific activity on the target compound rac-ketoprofen ethyl ester was calculated to be about 85, 000 unit, and the kinetic constants Km and Vmax were determined to be 0.2 mM and 90 mM/mg-protein/min respectively. The deduced amino acid alignment with the wild type enzyme revealed five mutations at L120P, I208V, T249A, D287H and T357A. Based on these observations, the site directed mutagenesis to delineate the mutagenic effect is under progress.

• BMB Reports
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• 제30권3호
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• pp.222-228
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• 1997

Protein phosphatase 2C (PP2C) is one of the four major serine/threonine phosphatases which is dependent on $Mg^{2+}$ for its activity. PP2C was purified from rat liver cytosol and its characteristics were investigated. The substrate employed for routine assay was $[^{32}P]casein$ phosphorylated by PKA. The purification process involved DEAE chromatography, ammonium sulfate fractionation, phenyl sepharose chromatography, sephacryl 5-200 gel filtration, and histone agarose chromatography. The SDS-PAGE of PP2C showed one major single protein band at a position corresponding to a molecular mass of 43 kd and the purification fold was 637. The enzyme showed a pH optimum of 8 and $K_M$ value was $1.9\;{\mu}M$. However, when the substrate was changed to $[^{32}P]histone$, the pH optimum was shifted to 7 and $K_M$ value was $2.3\;{\mu}M.\;Mg^{2+}$ was essential to the enzyme activity and okadaic acid did not exert any inhibitory effect on the enzyme. To examine residue in the active site of PP2C effects of some protein-modifying reagents were tested.

• 한국환경과학회지
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• 제9권5호
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• pp.437-441
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• 2000

A study on the removal of Pb ion from Pb-contaminated soil was carried out using ex-site extraction process. Tartaric acid (TA) and iminodiacetic acid sodium salt(IDA) as a washing agent were evaluated as a function of concentration reaction time mixing ratio of washing agent and recycling of washing agent. TA showed a better extraction performance than IDA. The optimum washing condition of TA and IDA were in the ratio of 1:15 and 1:20 between soil and acid solution during 1 hr reaction. The total concentrations of Pb ion by TA and IDA at three repeated extraction were 368.8 ppm and 267.5 ppm respectively. The recovery of Pb ion from washing solution was achieved by adding calcium hydroxide and sodium sulfide form the precipitation of lead hydroxide and lead sulfide and optimum amounts of sodium sulfide and calcium hydroxide were 7 g/$\ell$ for the TA washing solution and 4 g/$\ell$, 5g/$\ell$ for the IDA washing solution respectively. The efficiency of recycle for TA and IDA washing solution were 78.8% , 95.1%, and 89.2%, 96.6% at third extractions under $Na_2S$ and $Ca(OH)_2$, respectively.

• 한국의류산업학회지
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• 제11권4호
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• pp.515-526
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• 2009

From traditional ceremony to science technology, origami was used as technique for artistic creativity and functionality based on formative characteristics. The purpose of this study is to analyze the expressive characteristics of functionality in fashion design using origami technique. The method of this study was to analyze recent documentaries, fashion books, fashion magazines, internet web site and so forth. The results were as follows: In various fields of design, the category of the expressive characteristics was classified as efficient correspondence of space, compositive convergence of function, variable potentiality of form and human ergonomic optimum. In recent origami fashion based on functionality, the efficient correspondence of space was expressed constructive or decorative pocket design, using on clothing surface and varied design by lifestyle. The compositive convergence of function was expressed innovative connection of fashion items or non-fashion items and deconstructed classical clothing pattern. The variable potentiality of form was expressed control of form by irregular fold, dynamic metamorphosis by organic fold and creation of varied clothing silhouette. The human ergonomic optimum was expressed metamorphosis mechanism from two dimension to three dimension, suitable construction to physical ability and limit and organic form based on articular folding. The functional origami fashion means reflection of nomadic lifestyle, maximum of usability, pursuit of playful sensibility, creation of new body aesthetic and pursuit of technology and humanism. And the result of these study may be used for creative inspiration in fashion design because of unique characteristics.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.318-324
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• 1998

A ${\beta}$-galactosidase with high transgalactosylic activity was purified from a Bacillus species, registered as KFCC10855. The enzyme preparation showed a single protein band corresponding to a molecular mass of 150 kDa on SDS-PAGE and gave a single peak with the estimated molecular mass of 250 kDa on Sephacryl S-300 gel filtration, suggesting that the enzyme is a homodimeric protein. The amino acid and sugar analyses revealed that the enzyme is a glycoprotein, containing 19.2 weight percent of sugar moieties, and is much more abundant in hydrophilic amino acid residues than in hydrophobic residues, the mole ratio being about 2:1. The pI and optimum pH were determined to be 5.0 and 6.0, respectively. Having a temperature optimum at $70^{\circ}C$ for the hydrolysis of lactose, the enzyme showed good thermal stability. The activity of the enzyme preparation was markedly increased by the presence of exogenous Mg (II) and was decreased by the addition of EDTA. Among the metal ions examined, the most severely inhibitory effect was seen with Ag (I) and Hg (II). Further, results of protein modification by various chemical reagents implied that 1 cysteine, 1 histidine, and 2 methionine residues occur in certain critical sites of the enzyme, most likely including the active site. Enzyme kinetic parameters, measured for both hydrolysis and transgalactosylation of lactose, indicated that the enzyme has an excellent catalytic efficiency for formation of the transgalactosylic products in reaction mixtures containing high concentrations of the substrate.

• 한국지진공학회논문집
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• 제6권5호
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• pp.29-36
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• 2002

교량의 내진성능확보를 위한 유지보수계획을 수립하는데 있어서, 전체 생애주기비용의 최소화를 통하여 최적의 검사 및 수리회수의 결정방법을 제시한다. 생애주기 비용에는 초기비용, 파괴비용 뿐만 아니라, 검사 및 보수비용이 포함된다. 시간에 따른 내진성능저하모델을 손상함수를 이용하여 표현하였으며, 손상감지정도에 따른 Event Tree Analysis를 통하여 유지보수에 따른 파괴확률을 랜덤진동이론을 이용하여 산출한다. 예제로서 10경간 연속교의 최적유지보수방법을 살펴본 결과, 가속도계수가 증가하고, 연약지반일수록 최적유지보수회수는 증가하는 것으로 나타났다.

• 제어로봇시스템학회논문지
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• 제4권4호
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• pp.464-478
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• 1998

In teleoperation, it is important for an operator to feel as if he really were in a distant place. To realize this objective, the various information from a remote site must be presented to the operator. Even though tactile information is very important to efficiently execute a task, it is not yet sufficiently provided for the operator. In this paper, we propose the new mechanism that can provide the more dexterous tactile information to the operator This device utilizing the electromagnetic force is designed to be compact and light enough to be attached to the fingerpad, and designed to be controlled continuously. The magnetic circuit is derived by the probable flux paths method in order to take forces at any given dimension. An optimization technique is also proposed to maximize the tactile force that humans can perceive under the same conditions. The objective function is formulated as maximizing displacements indented on the fingerpad, considering the mechanism of human tactile perception. The optimization formulation is subject to the geometric and rising temperature constraints in the coil. It is demonstrated that, by optimization, the tactile force increases by 24%, compared with that obtained from the initial design.