• Title/Summary/Keyword: optimum pH

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Characteristics of Apparent Molecular Weight Distribution and Removal of DOC by Coagulation and Sedimentation Process with Polyaluminum Chloride in Nakdong River Water (응집·침전 공정에서 PAC를 이용한 낙동강 원수의 DOC 제거 및 분자량 분포 특성)

  • Kim, Yeong-Tak;Kim, Eun-Hee;Rhim, Jung-A;Yoon, Jeong-Hyo;Kim, Dong-Youn
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.1
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    • pp.125-133
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    • 1999
  • The objective of present work is to evaluate the optimum coagulation conditions in order to decrease dissolved organic carbon(DOC) and turbidity at different polyaluminum chloride dosage and pH from Nakdong River water. This studies were carried out to examine distribution on apparent molecular weight(AMW) of DOC in the Nakdong River water and its coagulation-sedimentation water. On the basis of jar tests, at the optimum coagulation pH in order to decrease DOC and turbidity were pH 5.0~6.0 and optimum dosage of polyaluminum chloride were 10~15mg $Al_2O_3/L$. The removal percentage of DOC and UV-254 absorbance were 35~40%, 45~60%, respectively. In pilot plant, at the optimum coagulation pH in order to decrease DOC and turbidity were 5.0-6.5, and the removal percentage of DOC were 30~45%. Distributions of AMW in the Nakdong River, less than 6,800dalton were 60.7% 6,800~11,000dalton were 32.8%, more than 11,000dalton were 6.4%. When the polyaluminum chloride dosage was 12~20mg/L, the removal percentages of each AMW for AMW of Nakdong River water, less than 6,800dalton were 25~28%, 6,800~11,000dalton were 65~68% more than 11,000dalton were 10~60%.

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Change of Functional Properties and Extraction of Sesame Meal Protein with Phytase and Protease (Phytase와 Protease 혼합처리에 의한 참깨박 단백질 용출 및 기능성 변화)

  • Chun, Sung-Sook;Cho, Young-Je;Cho, Kuk-Young;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.895-901
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    • 1998
  • To extract insoluble proteins and improve functional properties of sesame meal proteins was treated with phytase and protease from Aspergillus sp. It was found that the optimum pH, optimum temperature, optimum treatment time and optimum unit of enzyme for extraction of protein were pH $10{\sim}12$ (alkaline), $60^{\circ}C$, 11 hr. and 900 units of phytase and 60 units of protease, respectively. The foaming capacity, foaming stability, oil absorption and water absorption of sesame meal protein after treatment with phytase and protease were increased as compared to the control.

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Feasibility Study on the Development of Fiber-Optic pH Sensor for Endoscope (내시경용 광섬유 pH 측정 센서 개발을 위한 기초 연구)

  • Lee, Bong-Soo;Hong, Ju-Young;Hwang, Young-Muk;Cho, Seung-Hyun
    • Journal of Sensor Science and Technology
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    • v.13 no.3
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    • pp.213-217
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    • 2004
  • The general method to find a H. pylori in the stomach is the rapid urease test but it is only used to decide the infection with H. pylori. In this study, it is tried to develope fiber-optic pH sensor which can be used with gastroscop to quantify H. pylori. To measure the degree of infection with H. pylori, the color change of phenol red according to the degree of pH is measured by optical fibers with different light sources and the optimum distance from a sample to the end of sensor tip is decided by measuring the maximum reflectivity from a sample. Also the sensitivity study is carried out to decide the optimum light source which has sensitive change of reflectivity to the change of pH. It is expected that the fiber-optic pH sensor which measures the degree of infection with H. pylori exactly can be developed.

Changes in Optimum pH and Thermostability of $\alpha$-amylase from Bacillus licheniformis by Site-directed Mutagenesis of His 235 and Asp 328

  • Kim, Mi-Sook;Lee, Sang-Kyou;Jung, Han-Seung;Yang, Chul-Hak
    • Bulletin of the Korean Chemical Society
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    • v.15 no.10
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    • pp.832-835
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    • 1994
  • The ${alpha}$-amylase gene of Bacillus licheniformis has been cloned and two mutant ${alpha}$-amylase genes of which histidine 235 was changed to glutamine (H235Q) and aspartic acid 328 to glutamic acid (D328E) have been produced by site-directed mutagenesis. The kinetic parameters, optimum pH and thermostability of wild type(WT) and these two mutant amylases expressed in E. coli MC1061 have been compared after purification. The $K_m$ values of WT, H235Q and D328E ${alpha}$-amylases were 0.22%, 0.73%, and 0.80% respectively, when using starch as the substrate. The $V_max$ values of wild type ${alpha}$ -amylase and mutant ${alpha}$-amylases were 0.6-0.7%/minute, and did not show any significant differences among them. The optimum pH of D328E ${alpha}$-amylase was shifted to more acidic pH. Also, the thermostability of H235Q ${alpha}$-amylase was increased compared to the wild type ${alpha}$-amylase.

Studies on the Cellulolytic Enzymes of Stachybotrys atra(I) (Stachybotrys atra에서 추출한 섬유소 분해효소에 관한 연구. I)

  • 김은수;김영민;강영희;최태주
    • Korean Journal of Microbiology
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    • v.13 no.2
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    • pp.59-63
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    • 1975
  • When the enzyme preparations were at various temperatures for 1 hour, the thermal stability for the enzyme was maximum at $30{\circ}C.$ The optimum temperature for the enzyme activity was at $40{\circ}C.$ When the enzyme preparations were exposed to various pHs for 22 hours, the enzyme stability was maximum at pH 3.8, and it was decreased gradually as the pH rose up to 4.8, above which the stability was greatly restored. When the exposure period was extended from 22 to pH's 3.0 and 5.9, but the stability tended to rise at pH's below 3.0 and above 5.9. The optimum pH for the enzyme activity was obtained at 4.8.

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A Study for the Optimum pH of Hydrogen Production in Anaerobic Batch Reactor (혐기성 회분반응기에서 수소생산 시 최적 pH 산정에 관한 연구)

  • Jun, Yoon-Sun;Park, Jong-Il;Yu, Seung-Ho;Lee, Tae-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.1
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    • pp.54-61
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    • 2007
  • The influences of pH were investigated for anaerobic hydrogen gas production under the constant pH condition ranged from pH 3 to 10. Carbon dioxide and hydrogen gas were main components of the gas but methane was not detected in the produced gas when sucrose was added in enrichment medium. When the modified Gompartz equation was applied for the statistical analysis of experimental data, a hydrogen production potential and maximum gas production rate at pH 5 were 1,182 mL and 112.46 mL/g dry wt biomass/hr. The hydrogen conversion ratio was 22.56%. The butyrate/acetate ratios at pH 5 and pH 6 are 1.63 and 0.38. Higher butyrate/acetate ratio produced more hydrogen gas generation. The Haldane equation model was used to find the optimum pH and fitted well with the experimental data$(r^2=0.98)$. The optimum pH and specific hydrogen production were 5.5 and 119.61 mL/g VSS/h.

Effect of Incubation Temperature and pH on Chlamydospores Germination of Cylindrocarpon destructans Causing Root Rot of Panax ginseng (인삼 뿌리썩음병균 Cylindrocarpon destructans의 후막포자 발아에 미치는 배양온도 및 pH의 효과)

  • 조대휘;유연현
    • Journal of Ginseng Research
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    • v.25 no.3
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    • pp.136-140
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    • 2001
  • Effects of incubation temperature and pH on chlamydospore germination of Cylindrocarpon destrcutans (isolate CY-9802) causing root rot of Panax ginseng were studied. Germination rate of the chlamydospores on Czapek solution agar(CSA) was higher than on potato dextrose agar(PDA) at the incubation temperatures tested. The chlamydospores were able to be germinated at range of 5$\^{C}$ to 30$\^{C}$ after 48 hours incubation on CSA. Germination rate was 53.2∼6.27% at range of 15$\^{C}$ to 25$\^{C}$, and the optimum temperature was 20$\^{C}$, whereas they were very low at 30$\^{C}$ on PDA. Germination rate was 43.6% to 47.9% at range of 10$\^{C}$ to 20$\^{C}$, and the optimum temperature was 20$\^{C}$ as well. They were able to be germinated at pH of 5.2 to 8.1 on CSA and 5.2 to 7.2 on PDA. Optimum pHs for the germination on CSA and PDA were from 6.4 to 8.2 and from 5.2 to 6.0, respectively. Mycelial color of the fungus on CSA was pale brown at pH from 5.2 to 6.0 and white from pH 6.4 to 8.1, while it was typical dark brown ar range of pH 5.2 to 7.1 and brown at pH 7.2 on PDA after 21 days incubation.

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Synthesis of $\beta$-Alumina By Oxalate Coprecipitation Method and Its Crystallization Behavior (Oxalate 공침법에 의한 $\beta$-Alumina 합성과 결정화 거동)

  • 박용민;양유철;김형욱;박성수;손영국
    • Journal of the Korean Ceramic Society
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    • v.32 no.4
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    • pp.455-461
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    • 1995
  • To investigate the synthesis of $\beta$-Al2O3 and its crystallization behavior by oxalate coprecipitation method, the optimum pH range for oxalate coprecipitates has been theoretically calculated from the solubility products and the equilibrium constans of each metal ionic species and their solubility diagram wa obtained. The optimum pH range for oxalate coprecipitates at room temperature was estimated as <4. In experiment, we found that the optimum condition for oxalate coprecipitates was pH<1, which was not doped with pH controller. The Na+ ions were easily exchanged for the NH4+ ions of NH4OH which was used as pH controller, and those NH4+ ions were supposed to affect the crystallization behavior of $\beta$-Al2O3. The thermal decomposition of all complexes was almost complete below 40$0^{\circ}C$. The primary product of the decomposition process was m-Al2O3, which transformed to $\beta$"- or $\beta$-Al2O3 at temperature higher than 100$0^{\circ}C$. We found that the powder prepared at 120$0^{\circ}C$ had only $\beta$"- and $\beta$-Al2O3.EX>-Al2O3.

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Estimation of the Optimum Factor of the Struvite Crystalization for the Nitrogen and Phosphorus Removal in WWTP (폐수내 질소 및 인 제거를 위한 struvite 결정화 최적조건 도출)

  • Kim, Ji Yeon;Moon, Yong Taik;Seo, In Seok;Kim, Byung Goon
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.6
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    • pp.745-753
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    • 2007
  • By struvite and hydroxyapatite crystallization, was high concentration of nitrogen and phosphorus in wastewater simultaneously. Particularly, removal of nitrogen and phosphate for crystallization have been applied to landfill leachates and animal wastewater. The purpose of this study is to decide the optimum struvite crystallization factors, sequence of $Mg^{2+}$ addition, pH control and the molar ratio of $Mg^{2+}$ over $PO_4^{3-}$. In conclusion, dosage of the magnesium followed by pH control formed magnesium hydroxide, so pH was decreased. Therefore, pH adjustment should followed by after magnesium dosage and then pH should be adjusted to 11. Over pH 10, it was not good for struvite crystallization efficiency by side reaction. Following of the $Mg^{2+}$ and the $PO_4^{3-}$ are dosed excessively, the removal efficiency of the $NH_4^+$ increased. A molar ratio of $Mg^{2+}:NH_4^+:PO_4^{3-}$, 1.3:1:1.3 was the most on effective for $NH_4^+$ removal at pH 9.5. But for the perfect removal $NH_4^+$, it is thought to be that molar ratio should be 2:1:2.

Clarification of Fruit Juice by the Use of Polygalacturonase and Gelatin (Polygalacturonase와 젤라틴을 병용한 과실쥬스의 청징화)

  • Hur, Byung-Suk;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.32 no.4
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    • pp.367-373
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    • 1989
  • The effect of gelatin concentration, pH of fruit juice and other factors for the enzymatic clarification of fruit juice by the use of polygalacturonase was studied. The proper addition of gelatin was much effected on clarification by polygalacturonase and optimum concentrations of gelatin for the enzymatic clarification of Zugaru juice, Magnolia Gold juice, Golden Delicious juice, Jonagold juice, Jonathan juice, Campbell Early juice were 0.04, 0.01% 0.02%, 0.06% 0.01%, and 0.04%, respectively. Optimum pH for the clarification by the use of polygalacturonase and gelatin was $3.2{\sim}3.5$ contrary to optimum pH 4.8 of polygalacturonase for the hydrolysis of pectic acid. At the reaction temperature of $45^{\circ}C$ and polygalacturonase concentration of 0.01mg/ml, fruit juices were completely clarified by optimum addition of gelatin for 60min.

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