• Microbiology and Biotechnology Letters
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• v.25 no.3
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• pp.298-304
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• 1997

Thermus caldophilus GK24 was selected as sources of thermostable $\beta$-galactosidase from a survey of genus Thermus. T. caldophilus GK24 (Tca) $\beta$-galactosidase was found to be inducible. The enzyme was optimally active at 75$\circ$C. Enzyme induction was achieved by addition of lactose, galactose and cellobiose to basal media. The addition of glucose to culture media had a repressive effect on further enzyme synthesis. T caldophilus GK24 was tested for production of $\beta$-galactosidase by addition of various concentration of lactose, galactose and cellobiose to standard media. Cellobiose was found to be effective for the $\beta$-galactosidase induction. The optimal induction medium for production of $\beta$-galactosidase was composed of 0.2% cellobiose, 0.3% bactotryptone, 0.3% yeast extract, basal salts and Tris/HCI(pH 7.8). The activity of the enzyme in the optimal induction medium increased nearly 16.5-fold compared to the standard medium. Tca $\beta$-galactosidase was detected when cell extracts was subjected to electrophoresis in a nondenaturing polyacryamide gel and stained for activity with 6-bromo-2-naphtyl-$\beta$-D-galactopyranoside(BNG).

• ETRI Journal
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• v.38 no.5
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• pp.952-961
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• 2016

In this paper, we study a spectrum sharing network (SSN) where a spectrum sharing device (SSD) coexists with multiple wireless communication systems (WCSs) in the same channel. The SSD can operate with either a duty cycle (DC) channel access mechanism or a listen-before-talk (LBT) channel access mechanism, whereas WCSs operate with an LBT mechanism. An opportunistic channel selection scheme for the SSD in the SSN is first proposed to minimize the outage probability. The optimal data transmission time for the DC-based SSD is derived to further improve the outage probability. We also derive the exact and closed-form outage probability of the proposed channel selection in the SSN by assuming that the number of WCSs operating in each channel is uniformly distributed. The simulation results show that the proposed channel selection scheme outperforms other channel selection schemes. It was also observed that a DC-based SSD with an optimal data transmission time provides a better outage performance than an LBT-based SSD. As the number of available channels increases, the channel selection scheme plays an important role in minimizing the outage probability of the SSNs.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.1
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• pp.151-156
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• 2023

This paper deals with the optimization problem that decides the number of advertising for any media among various medium to maximize the perception quality index of new product meets the given budget and over the minimum reached people constraints. For this problem, there is only in used the mathematical approach as linear programming (LP) software package and has been unknown the polynomial time algorithm. In this paper we suggest the heuristic algorithm with O(nlog n)time complexity to solve the optimal solution for this problem. This paper suggests the evaluation index to select the media most economically-efficient way and decides the media and the number of advertisement. While we utilize Excel, the proposed algorithm can be get the same optimal solution as LP for experimental data.

• ETRI Journal
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• v.24 no.4
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• pp.280-289
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• 2002

In this work, we present a novel approach to the bit allocation problem that aims to minimize overall distortion subject to a bit rate constraint. The optimal solution can be found by the Lagrangian method with dynamic programming. However, the optimal bit allocation for block-based interframe coding is practically unattainable because of the interframe dependency of macroblocks caused by motion compensation. To reduce the computational burden while maintaining a result close to the optimum, i.e., near optimum, we propose an alternative method. First, we present a partitioned form of the bit allocation problem: a "frame-level problem" and "one-frame macroblock-level problems." We show that the solution to this new form is also the solution to the conventional bit allocation problem. Further, we propose a bit allocation algorithm using a "two-phase optimization technique" with an interframe dependency model and a rate-distortion model.

• Journal of Embryo Transfer
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• v.14 no.1
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• pp.39-46
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• 1999

To establish the optimal culture systems for production of transferable embryos in Korean Cattle, pregnancy rates of IVF-derived blastocysts according to different culture media, culture method and culture duration were compared. Development of IVF-derived embryos to blastocysts was most effective in YS medium group co-cultre with cumulus cells. Blastocysts cultured for 6 to 8 d in vitro showed higher hatching rate and good quality. Pregnancy rates after transfer of IVF-derived blastocysts cultured for 7 or 8 d were high. Through our experiments, it is considered that improvement of culture media and culture method is necessary for mass production of blastocysts with excellent of good quality in Korean Cattle.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.1
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• pp.7-12
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• 2010

To determine the optimal isolation conditions of the entomopathogenic fungi from soil, we compared their growth characteristics with non-entomopathogenic fungi on agar media containing various concentrations of cooper (II) chloride ($CuCl_2$) or dodine. The result showed that dodine medium is more selective, and the optimal concentration of dodine is determined with $50{\mu}g$/ml. We could isolate several putative entomopathogenic fungi from soil using this, and identify them using ITS analysis. As a result, 64% fungi were identified as typical entomopathogenic fungi. This selective medium may be useful to the rapid and simple isolation of entomopathogenic fungi from soil.

• Journal of Microbiology
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• v.43 no.1
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• pp.38-43
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• 2005

Lactic acid production parameter optimization using Lactobacillus amylovorus NRRL B-4542 was performed using the design of experiments (DOE) available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, moisture, yeast extract, $MgSO_4{\cdot}7H_20$, Tween 80, and corn steep liquor (CSL) were determined. Among the physical parameters, temperature contributed higher influence, and among media components, yeast extract, $MgSO_4{\cdot}7H_20$, and Tween 80 played important roles in the conversion of starch to lactic acid. The expected yield of lactic acid under these optimal conditions was 95.80% and the actual yield at optimum conditions was 93.50%.

• Smart Media Journal
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• v.10 no.2
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• pp.48-54
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• 2021

Sepsis is one of the leading causes of mortality globally, and it costs billions of dollars annually. However, treating septic patients is currently highly challenging, and more research is needed into a general treatment method for sepsis. Therefore, in this work, we propose a reinforcement learning method for learning the optimal treatment strategies for septic patients. We model the patient physiological time series data as the input for a deep recurrent Q-network that learns reliable treatment policies. We evaluate our model using an off-policy evaluation method, and the experimental results indicate that it outperforms the physicians' policy, reducing patient mortality up to 3.04%. Thus, our model can be used as a tool to reduce patient mortality by supporting clinicians in making dynamic decisions.

• Korean Journal of Animal Reproduction
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• v.16 no.3
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• pp.209-215
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• 1992

These studies were carried out to overcome 2-cell block and in vitro development to blastocysts in vitro fertilization of mouse embryos. The unfertilized ova were obtained by superovulation in ICR mice of 4 to 6 weeks old. Tyrode's 280 solution was used as basal media, and the pH range of media examined was designed from 6.5 to 7.5 with 0.2 interval and the range of osmolality from 250 to 370 mOsm with 20 interval, and the period of sperm preincubation examined was 30, 60, 120, and 180 minutes. The ova developed to 2-cell embryos after 26hrs of incubation with preincubated sperm were evaluatated as in vitro fertilized ones. The results obtained were summarized as follows: 1. The optimal ranges of pH and osmolality of culture media and of sperm preincubation time for in vitro development of in vitro fertilized ova to blastocyst were pH 7.1 to 7.3, 250 to 350 mosmol and 60 to 180 min, respectively. 2. With the media of pH 7.1, 310 mOsm and sperm preincubation period of 120min in another experiment of large sample size, the in vitro fertilized ova was found 66.5% and the in vitro development of in vitro fertilized ova to blastocyst was found 35.8%. From the above results it was concluded that the optimal conditions of pH and osmolality of the media for mouse IVF and embryo culture, and the period of sperm preincubation might be 7.1, 310 mOam and 120min, respectively.

• Clinical and Experimental Vaccine Research
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• v.12 no.4
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• pp.328-336
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• 2023

Purpose: Human peripheral blood mononuclear cell (PBMC)-based in vitro systems can be of great value in the development and assessment of vaccines but require the right medium for optimal performance of the different cell types present. Here, we compare three commonly used media for their capacity to support innate and adaptive immune responses evoked in PBMCs by Toll-like receptor (TLR) ligands and whole inactivated virus (WIV) influenza vaccine. Materials and Methods: Human PBMCs were cultured for different periods of time in Roswell Park Memorial Institute (RPMI), Dulbecco's minimal essential medium (DMEM), or Iscove's modified DMEM (IMDM) supplemented with 10% fetal calf serum. The viability of the cells was monitored and their responses to TLR ligands and WIV were assessed. Results: With increasing days of incubation, the viability of PBMCs cultured in RPMI or IMDM was slightly higher than that of cells cultured in DMEM. Upon exposure of the PBMCs to TLR ligands and WIV, RPMI was superior to the other two media in terms of supporting the expression of genes related to innate immunity, such as the TLR adaptor protein gene MyD88 (myeloid differentiation factor 88), the interferon (IFN)-stimulated genes MxA (myxovirus resistance protein 1) and ISG56 (interferon-stimulated gene 56), and the leukocyte recruitment chemokine gene MCP1 (monocyte chemoattractant protein-1). RPMI also performed best with regard to the activation of antigen-presenting cells. As for adaptive immunity, when stimulated with WIV, PBMCs cultured in RPMI or IMDM contained higher numbers of IFNγ-producing T cells and secreted more immunoglobulin G than PBMCs cultured in DMEM. Conclusion: Taken together, among the different media assessed, RPMI was identified as the optimal medium for a human PBMC-based in vitro vaccine evaluation system.