The aim of this study is to select the superior resources of high yield, high content of functional material optimal to mechanical harvesting by the evaluation of crop growth and yield characteristics in sorghum germplasm. One hundred accessions of sorghum germplasm were used in this experiment. Days from seeding to heading date showed the range from 68 to 94 days with the highest frequency proportion was the group from 80 to 85 days, which occupied 34% (34 plant resources) of 100 germplasm. Ear types of 100 sorghum germplasm could be classified as 7 types of broom-tillering, half broom-tillering, extreme open-loose type, open-loose type, intermediate type, compact type, extreme-compact type of which intermediate type was the highest ratio of 28% (28 plant resources) of 100 germplasm. Yield showed the range from 106 to 365 ㎏/10a with the highest frequency proportion of it was the group from 150 to 200 ㎏/10a, which occupied 44% (44 plant resources) of 100 germplasm. Among 100 sorghum germplasm, 18 ideal resources of high yield and short plant height appropriate for mechanical harvesting were selected. In order to evaluate high content of functional substance, selected 18 resources were analyzed for total polyphenol content, DPPH radical scavenging activity and total anthocyanin content. Finally, we selected 5 resources of short plant height, high yield, high content of total polyphenol and high DPPH radical scavenging activity among 18 genetic resources.
In order to obtain high-level production of recombinant human thrombopoietin (rhTPO) in insect cell line, HTI-TN-5B1-4 (TN5), conditions for optimal rhTPO expression such as multiplicity of infection (MOI), the cell density at infection, harvesting time and type of culture method as well as growth media were determined. When TN5 cells were cultured as anchorage-dependent state in 60-mm dish, cell density $2\times^6$ cells,MOI of 10 and Garvesting the culture media at 72 hr post-infection wrere the cinditions for highest rh TPO production. High production of rhTPO was also achieved by using EXPRESS FIVE serum free media rather than SF900II serum free media-1. Anchorage-dependent TN5 cells were adapted as a suspension culture when they were grown in the presence of heparin. TN5 cells were successfully cultured at 0.2 L scale in suspension culture without having aggregation. When TN5 cells were cultured as suspension state, cell density of $0.6\times10^6$ cells/mL, MOI of 1 and harvesting the culture media at 72 hr post-infection, gave the highest yield of rhTPO.
Han, Won Sung;Han, Han-Sup;Kim, Nam-Hun;Cha, Du Song;Cho, Koo Hyun;Min, Do Hong;Kwon, Ki Cheol
Journal of Korean Society of Forest Science
/
v.103
no.1
/
pp.87-97
/
2014
This study was conducted to provide field-based harvesting study information which can be used to select an optimal cable system for certain work conditions on steep grounds (> $20^{\circ}$ ground slope) in Korea. To accomplish this study objectives, we evaluated three cable yarding systems (RME-300T tower-yarder, Chuncheon tower-yarder, FARMI tractor winch) working in typical work conditions for their yarding productivity and operational efficiency. Those yarders are commonly used for removing logs or trees on steep grounds in Korea. Under the same work conditions (average DBH of tree to be cut, 20 cm; yarding distance, 60 m; lateral yarding distance, 10 m; and machine utilization rate, 70%), the average productivities were $33.04m^3$/day, $38.47m^3$/day, and $14.17m^3$/day for RME-300T, Chuncheon tower-yarder, and FARMI, respectively. Our standardized cost comparison study also showed that the yarding cost was highest at $37,835won/m^3$ with FARMI, followed by RME-300T at $25,105won/m^3$ for the same work conditions. We found the lowest yarding cost with the Chuncheon tower-yarder at $20,520won/m^3$ which was resulted primarily from high yarding productivity at the yarding distance (60 m). Our analysis suggested that a small machine such as FARMI could be a low-cost yarding machine option for a cable yarding job with a short yarding distance (40 m or less). The Chuncheon tower-yarder is well suited for a mid-range yarding distance job in Korea, ranged between 40 to 140 m. If yarding distance were longer than 140 m, the RME-300T tower yarder appears to be most cost-effective.
Young Sang Yu;Li Li Wang;Yan Fen Li;Xaysana Panyavong;Bae Hun Lee;Jong Geun Kim
Journal of The Korean Society of Grassland and Forage Science
/
v.44
no.2
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pp.64-70
/
2024
The experiment was conducted to determine the changes in seed productivity of Italian ryegrass (Lolium multiflorum Lam.) according to nitrogen fertilization levels in the southern region of Korea. Italian ryegrass (IRG) variety 'Green Call' was sown in the fall of 2021 in Jinju, Gyeongsangnam-do. The experiment consisted of three nitrogen fertilizer levels (100, 120, and 140 N kg/ha) with three replications using a randomized complete block design. Harvesting was done approximately 30 days after heading on May 18th. There was no difference in heading date among treatments, which occurred on April 18th. The longest IRG was observed in the 140 N kg/ha treatment, but there was no significant difference. No significant differences were observed in lodging, disease resistance, and cold tolerance among treatments, but lodging was severe in all treatments. The length of the spike averaged 44.95 cm, with no difference among treatments, and the number of seeds per spike was highest in the 120 N kg/ha treatment. Seed yield increased with increasing nitrogen fertilizer levels, averaging 3,707 kg/ha (as-fed basis). DM content of seed and straw averaged 76.95% and 62.19%, respectively, with no significant differences among treatments. The remaining straw after harvesting averaged 6,525 kg/ha on a dry matter basis, with the highest value observed in the 140 N kg/ha treatment. Overall, considering the results, the optimal nitrogen fertilizer application rate for seed production of Italian ryegrass in the southern region when sown in autumn was found to be 120 N kg/ha.
In spite of various merit of algae as biofuel, the production cost of algae is a considerable obstacle for commercialization. The concurrent development of essential technologies is needed for the cultivating, harvesting, extracting and energy transformation. The production cost of algae biofuel has still higher than that of the other commercial biofuel. The major research activity has been focused on the cultivating and the research of other processes has been done with relatively lower activity. It is difficult to separate the algae from water because of the similar magnitude of density each other. The agglomeration and extracting of algae with the hybrid technology using ultrasonic wave is rare effect of environmental hazard and also it is appropriate technology for the next generation energy resources. The present research is investigated for the effective separation of algae from water with the ultrasonics wave. The aim of the present research is focused on the establishment of optimal design of algae agglomeration system. For this purpose, the computational fluid dynamic analysis has been conducted in the flow field with ultrasonic wave and algae flow to clarify the mechanism of algae separation by ultrasonic wave.
Jo, Woo-Sik;Lee, Sung-Hak;Park, Woo-Ram;Shin, Seung-Ho;Park, Chang-Min;Oh, Ji-Hyun;Park, Who-Won
Journal of Mushroom
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v.15
no.4
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pp.264-268
/
2017
In the 21st century, information and communication technology (ICT) worldwide presents a new vision for agriculture. Time and place, as well as the high-tech industry, to overcome barriers to the fusion of the so-called "smart agriculture," are changing the agricultural landscape. Core container production in precision agriculture for mushroom cultivation, optimal temperature, humidity, irradiation, self-regulation of factors such as carbon dioxide, and environment for mushroom cultivation were adopted. Lentinula edodes (shiitake) is an edible mushroom native to East Asia, cultivated and consumed in many Asian countries. It is considered to be medicinal in certain practices of traditional medicine. We used different controlled light sources (Blue-Red-White-combined LED, blue LED, red LED, and fluorescent light) with different LED radiation intensities (1.5, 10.5, and $20.5{\mu}mol/m^2s$ for LEDs) to compare growth and development. Mushrooms were treated with light in a 12-hour-on/12-hour-off cycle, and maintained in a controlled room at $19{\sim}21^{\circ}C$, with 80~90% humidity, and an atmospheric $CO_2$ concentration of 1,000 ppm for 30 days. Growth and development differed with the LED source color and LED radiation intensity. Growth and development were the highest at $10.5{\mu}mol/m^2s$ of blue LED light. After harvesting the fruit bodies, we measured their weight and length, thickness of pileus and stipe, chromaticity, and hardness. The $10.5{\mu}mol/m^2s$ blue-LED-irradiated group showed the best harvest results with an average individual weight of 39.82 g and length of 64.03 mm, pileus thickness of 30.85 mm and pileus length of 43.22 mm, and stipe thickness of 16.96 mm with fine chromaticity and hardness. These results showed that blue LED light at $10.5{\mu}mol/m^2s$ s exerted the best effect on the growth and development of L. edodes (shiitake) mushroom in the ICT-system container-type environment.
Purpose: In near infrared spectroscopy, interactance configuration of a light source and a spectrometer probe can provide more information regarding fruit internal attributes, compared to reflectance and transmittance configuration. However, there is no through study on the parameters of interactance measurement setup. The objective of this study was to investigate the effect of the parameters on the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from greenhouses at three different harvesting seasons. The prediction models were developed at three distances of 2, 5, and 8 cm between the light source and the spectrometer probe, three measurement points of 2, 3, and 6 evenly distributed on each sample, and different number of fruit samples for calibration models. The performance of the models was compared. Results: In the test at the three distances, the best results were found at a 5 cm distance. The coefficient of determination ($R_{cv}{^2}$) values of the cross-validation were 0.717 (standard error of prediction, SEP=$1.16^{\circ}Brix$) and 0.504 (SEP=4.31 N) for the estimation of SSC and firmness, respectively. The minimum measurement point required to fully represent the spectral characteristics of each fruit sample was 3. The highest $R_{cv}{^2}$ values were 0.736 (SEP=$0.87^{\circ}Brix$) and 0.644 (SEP=4.16 N) for the estimation of SSC and firmness, respectively. The performance of the models began to be saturated when 60 fruit samples were used for developing calibration models. The highest $R_{cv}{^2}$ of 0.713 (SEP=$0.88^{\circ}Brix$) and 0.750 (SEP=3.30 N) for the estimation of SSC and firmness, respectively, were achieved. Conclusions: The performance of the prediction models was quite different according to the condition of interactance measurement setup. In designing a fruit grading machine with interactance configuration, the parameters for interactance measurement setup should be chosen carefully.
The objective of this field experiment was to investigate the growth, hay yield and chemical composition of cassava and stylo 184 grown under intercropping. The experiment was arranged in a Randomized Complete Block (RCB) design with 5 treatments and 4 replications. The treatments were: sole crop cassava (C); sole crop stylo 184 (S) and three intercropping treatments comprising an additive series of one (SC), two (SSC) and three (SSSC) rows of stylo 184 to one row of cassava. The results showed that leaf area per plant (LA) of cassava was significantly higher (p<0.05) in the sole crop relative to the intercropping treatments. Both total hay yield and CPDM yield were significantly higher (p<0.05) for C treatment and lower (p<0.05) for SSSC treatment. The total hay yield and CPDM yield were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments. At the first and second harvests, CP content was similar among treatments; while at third and fourth harvests, CP contents were significantly greater (p<0.05) for the intercropping treatment relative to the sole crop. At the first and second harvest, NDF contents were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments, whereas NDF contents were similar among intercropping treatments. Leaf area of stylo 184 at first and second harvest were significantly greater (p<0.05) for C, SC and SSC as compared with the SSSC treatments. At each harvesting, there were no significant differences in ash, CP, NDF, ADF and ADL contents of stylo 184 hay between the sole crop and intercropping treatments, except for the first harvest. ADF contents were significantly greater (p<0.05) in S, SC treatments relative to SSC and SSSC treatments. Both collective hay yield and CPDM yield of cassava and stylo 184 were significantly greater (p<0.05) for the SSC treatment and significantly lower (p<0.05) for the S treatment. Collective hay yield and CPDM yield were significantly greater (p<0.05) for the intercropping treatments relative to the sole crop. Based on this research, it was concluded that stylo 184 showed potential for intercropping with cassava. Intercropping cassava with stylo 184 has beneficial effects and can improve foliage biomass yield and soil fertility, which would be a more sustainable system than growing the cassava as a pure stand. In terms of hay yield and CP production, two rows of stylo 184 to one row of cassava could be the optimal pattern for this intercropping system.
Lee, Chae Young;Park, Jae Seong;Lee, Hee Du;Choi, Ye Seul;Hong, Seong Taek;Hong, Eui Yon;Lee, Yun Sang;Kim, In Jae;Woo, Sun Hee
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
/
pp.368-368
/
2017
Rice is the world's three major crops that is the staple food of Asian. Japonica type rice is consumed in Korea. Rice cultivation area decreases steadily. That is affected by decrease of the farm income with the inventory of 1.7 million tons, which is 2 to 3 times more than the optimal amount due to the decrease in the consumption of rice per person and cultivate high yield varieties. In recent years, as income level has improved, interest in health has been high and consumption for health food has been steadily increasing. For health food, rice is added by adding grains such as millet, sorghum, oats, beans and colored rice rather than white rice. In 1997, RDA(Rural Development Administration) developed black rice 'Heuknambyeo', and then 20 varieties were bred until 2017. In CBARES(Chungbuk Agricultural Research and Extension Services), we have developed new rice varieties 'Cheongpungheukchal' in 2010, 'Cheongpungheukhyangchal' in 2014, 'Cheongpungheukchal' is high in farming preference because of high yield. Black rice has high content of GABA and water-soluble phenol, DPPH radical scavenging activity, and consumers are interested in the function in the body. Therefore, functional and antioxidant activities(anthocyanin, total polyphenol, ${\alpha}-glucosidase$ inhibition) and antioxidant activities(ABTs, DPPH) were analyzed by comparing white and black rice. Testing varieties are 'Chucheongbyeo', best quality cultivars 'Daebo' and 'Samgwang' as white rice which are cultivated much in Chungbuk area, and black rice are 10 varieties including 'Cheongpungheukchal', 'Cheongpungheukhyangchal', 'Josaengheukchal' and so on. It has transplanted on 25th May, at CBARES research paddy by 100% fertilizer recommendation rate. Harvesting time was 50 days after heading by varieties, and has researched growth properties, yield and yield components, functional and antioxidant activities. Anthocyanin content was not measured because there was no pigmented in white rice, the highest value of anthocyanin content was 'Shintoheukmi' and in the range of 125.6~249.6mg/100g by black rice varieties. Total polyphenol content was high 'Cheongpungheukchal' and 'Shintoheukmi' and in the range of 96.68~244.34 mg/100g in black rice, white rice lower than blackish rice at 19.84~22.51mg/100g. ${\alpha}-glucosidase$ inhibition was high 'Cheongpungheukchal', 'Cheongpungheukhyangchal' and 'Shintoheukmi' in the rage of 75.87~98.85% by black rice varieties, especially 'Samgwang' was 80.75% and the other white rice was higher than 58~68%.
This study was conducted to evaluate an early-maturing rice cultivars adaptable to early transplanting cultivation in Yeongnam plain area. High temperature during the ripening stage of paddy rice under the early-transplanting cultivation in Yeongnam plain was to influence in several agronomic traits such as number of spikelet, ripening ratio, grain appearance of milled rice, and yield. 'Gounbyeo' and 'Junghwabyeo' were better than the others in grain appearance of milled rice and ripening ratio, but their yield potential were lower than that of the other varieties. Moreover, 'Gounbyeo' and 'Junghwabyeo' showed high ratio of broken rice. 'Unkwangbyeo' was the highest in yield and eating quality among the tested varieties, but also high percentage of white core and belly in milled rice. Therefore, in order to recommend those cultivars to farmers, it should be needed to establish an optimal cultivation method, such as transplanting density, fertilizer application, and harvesting time, etc. consequently, it should also be required to develop a new varieties with high ripening capability under high temperature condition.
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