• Korean Journal of Microbiology
• /
• v.29 no.6
• /
• pp.387-391
• /
• 1991

Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t$t_{d}$ =6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) ( $t_{d}$ =8h ) or at 20.deg.C ( $t_{d}$ =8h ). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (( $t_{d}$ =7h ) and pH 8.0 ($t_{d}$ =7.3h). the cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm ( $t_{d}$ =8.8h ) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.

• KSBB Journal
• /
• v.14 no.1
• /
• pp.71-75
• /
• 1999

A highly effective phosphorus accumulating bacterium named Acinetobacter CW3 was isolated from the nature by using Winogradsky columns. The optimal cultivation conditions of Acinetobacter CW3 in shaking flask were determined as $20^{\circ}C$, pH 7, 200rpm, 18.5mg $PO_4$-P/L. Acientobacter CW3 could remove phosphorus completely in 12hours for a batch culture at optimal cultivation condition. This bacterium could uptake phosphorus on aerobic condition and release it on anaerobic condition.

• Journal of applied mathematics & informatics
• /
• v.22 no.1_2
• /
• pp.425-434
• /
• 2006

The process of producing 1,3-preprandiol by microorganism continuous cultivation would attain its equilibrium state. How to get the highest concentration of 1,3-propanediol at that time is the aim for producers. Based on this fact, an optimization model is introduced in this paper, existence of optimal solution is proved. By infinite-dimensional optimal theory, the optimal condition of model is given and the equivalence between optimal condition and the zero of optimality function is proved.

• The Korean Journal of Mycology
• /
• v.35 no.2
• /
• pp.76-80
• /
• 2007

This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and investigated its optimal culture condition. Among four kinds of mushroom culture media, PDA medium was selected as the suitable culture medium. The optimal conditions for the mycelial growth of G. frondosa in PDA medium were $25^{\circ}C$ and $4{\sim}5$ of pH, respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively, and the highest mycelial growth was observed when the C/N ratio was $10{\sim}20$.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.26 no.4
• /
• pp.620-624
• /
• 1997

The conditions for color intensity and electron donating ability to $\alpha$,$\alpha$-diphenyl-$\beta$- picryl-hydrazyl (DPPH) of Bacillus subtilis DC-2 were investigated. Temperature, pH and cultivation time were chosen as three factors, and the optimal conditions of color intensity and DPPH was determined with response surface methodology. Color intensity was affected by cultivation temperature(p<0.1). DPPH was influenced by cultivation temperature(p<0.05) and pH(p<0.1). But cultivation time was affected neither color in- tensity nor DPPH. Optimal conditions of color intensity with Bacillus subtilis DC-2 were appeared at cultivation temperature of 39.$25^{\circ}C$, pH 8.83 and cultivation time of 84.41hrs. Optimal conditions of DPPH with Bacillus subtilis DC-2 were revealed at cultivation temperature of 39.19$^{\circ}C$, pH 8.84 and cultivation time of 82.21hrs.

• Journal of the Korean Society of Tobacco Science
• /
• v.5 no.1
• /
• pp.73-78
• /
• 1983

In order to investigate the relation between weather factors, soil water, and tobacco yield grown by nonnulching and mulehing cultivations, a regression analysis was conducted for data collected from 8 years (1971 to 1978) for a flue-cured variety of Hicks. The soil water was calculated by Smith's method. 1 . Tobacco yield was largely correlated to soil water deficit during draught time for nonmulclung cultivation; $Y=6.146+8.6185\times-0.0925$\times2(R=0.935)^{***})$ 2. Tobacco yield was more largely correlated to soil water in flexible draght time interval than in fixed time interval during maximum growing phase.3. This field test was supposed that the optimal soil water condition was 65%field caps city. In this condition tobacco yield was 197. 1-216.5kg/10a for non-mulching cultivation. But the soil water deficit in draught season was little matter for mulching cultivation. The relation between xield and evaporation during May to June was Y: -1199.55+9.4 353$\times$:O. 0155$\times$2 (R=0.904") Maximum tobacco yield was expected to 223.6-251.4kg/10a for mulching cultivation. 5 . Tobacco required high temperature and light even in drying season (maximum growing phase) for mulching cultivation.tion.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.6
• /
• pp.1020-1026
• /
• 1994

Pseudomonas plycolor was used to investigated the optimal culture condition to examine the various properties of superoxide dismutase (SOD). this SOD was inhibited by $H_2O_2$, azide ion, but not by cyanide ion. This result indicates that the enzyme might be a Fe-SOD. The composition of optimal culture medium for the enzyme production was 3% of glycerin, 1% of polypeptone, 0.5% of meat extract, 0.2% of KCI and the initial ph was 9.0 . The cultivation for the enzyme production was carried out in 500ml shaking flask containing 100ml of the optimal medium at $30^{\circ}C$ on a reciprocal shaker. The enzyme production reached maximum at 15hrs of cultivation and then declined sharply afterward.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.337-337
• /
• 2017

Accurate and optimal water supply to cereal crop is critical in growing stalks and producing maximum yields. Excessive soil moisture may cause nutrient deficiencies and oxygen deficiency. Excessive soil water during crop growth stages results in decrease of yields. In Korea, the largest agricultural lands are paddy fields. Recently, upland crops are cultivated in paddy field soils to reduce overproduced rice in Korea. In order to success this policy, it is necessary to fully understand crop response to excessive soil moisture condition from paddy field soils. Adzuki bean is one of major legumes which provide protein in daily diet. Adzuki bean has been well know its weakness to excessive soil moisture condition, In order to obtain optimal yields of adzuki bean from paddy field cultivation, it is necessary to understand response of adzuki bean under different soil moisture conditions. This study investigated characteristics of growths, yields and response degree of water stress from adzuki bean. Three cultivars were selected for this study; Chungju, Hongeon, and Arari. All adzuki beans were cultivated in a paddy field which was divided into three sections with different soil moistures. The paddy field was located in Milyang, Gyeongsangnam during 2016. One section of the paddy field had the greatest average soil moisture content as 35.1% during adzuki bean cultivation (very poor). The second greatest soil moisture section had 32.6% (somewhat poor) and the smallest soil moisture section had 28.9% of soil moisture (somewhat well). During cultivation of three cultivar adzuki beans, soil moisture contents and groundwater levels were monitored. All the characteristics of growth and yield components were measured; height, thickness, 100 seed weights etc. Stress index values were calculated by Stress Day Index (SDI). All cultivars had the greatest yields from somewhat well section. Chungju had the greatest yields throughout all three sections compared to other cultivars. Chungju had 81% greater yield than Hongeon which had the smallest yield from somewhat well section. Arari set in middle from all sections. However there was no significant differences yields from very poor and somewhat poor sections. Leaf SPAD values tended to decrease and stable carbon isotope values increased as soil moisture increased. However, Chungju had no difference across different soil moistures in SPAD and stable carbon isotope values, while Hongeon had the greatest differences across sections. These trends followed by SDI values. Chungju had the smallest SDI values compared to other cultivars, which meant that Chungju was the strongest tolerance against excessive soil moisture than other cultivars. All three cultivars showed severe decrease of yields from very poor and somewhat poor sections. Arari and Hongeon showed great decrease from somewhat well section compared to yields from upland soil. These two cultivars may not be proper cultivating in paddy fields. In conclusion, adzuki bean is very sensitive to soil moisture condition and detailed soil managements are required to obtain optimal yields of adzuki bean from paddy field cultivation.

• Microbiology and Biotechnology Letters
• /
• v.29 no.3
• /
• pp.134-141
• /
• 2001

A bacterial strain KP-364 producing extracellular keratinolytic protease was isolated from the soil of the poultry fac-tory. It was identified as Pseudomonas sp. based on its morphological and physiological characteristics, The optimal culture conditions for the production of keratinolytic protease by Pseudomonas sp. KP-364 were investigated. The composition of optimal medium for the keratinolytic protease was 2.0% glucose, 0.5% soybean meal. 0.5% $NaNO_3$ and 0.2% KCI Optimal initial pH for production of Keratinolytic protease production were 6.5 and $37^{\circ}C$ respec- tively. The keratinolytic protease production reached a maximum of 1,270 U/ml/hr after 48 hours cultivation under the optimal culture conditions.

• Environmental Engineering Research
• /
• v.20 no.4
• /
• pp.377-382
• /
• 2015

The high concentration of nitrogen and phosphorus in wastewater incorporated with the ability to use carbon dioxide as the carbon source make the microalgae become more attractive in wastewater treatment process. This study evaluates the optimal conditions for the digestion of settelable solids from the recirculating aquaculture system to produce the biomass of the green microalga Scenedesmus sp. After solids separation, aerobic digestion of settleable solids under disperse condition produced nitrate as the final product of consequently ammonification and nitrification processes. With the optimal digestion procedure, nitrate concentration during aerobic digestion in 2000 mL vessel increased from $9.63{\pm}0.65mg\;N/L$ to $58.66{\pm}0.06mg\;N/L$ in 10 days. Thereafter, cultivation of Scenedesmus sp. was performed in 1000 mL Duran bottle with air bubbling. The highest Scenedesmus sp. specific growth rate of $0.321{\pm}0.01/d$ was obtained in treatment using liquid fraction after aerobic digestion as the whole culture medium for Scenedesmus sp. cultivation. With this study, digestion of $8,800{\pm}128.12mg\;dry\;weight/L$ of settleable solids from fish pond finally produced $1,235{\pm}21mg\;dry\;weight/L$ of Scenedesmus sp. biomass.