• Toxicological Research
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• 제17권
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.

• Journal of the Optical Society of Korea
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• 제13권3호
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• pp.335-340
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• 2009

Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of $2{\mu}m$ to $5{\mu}m$ were fabricated on a $10{\mu}m$ thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

• 통합자연과학논문집
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• 제1권1호
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• pp.1-4
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• 2008

We developed quantification method based on the concept of candela in physics. The measurement of fluorescence signal from a nude mouse in the research of molecular biology. In the measurement of the optical signal with CCD, the quantification method for photon counts based on bio-luminescence imaging technique can provide comparative reference data. In this paper, we described theoretical derivation of our proposed concept. We hope this method could be a useful standard reference for quantitative date analysis in optical imaging.

• Current Optics and Photonics
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• 제2권6호
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• pp.595-605
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• 2018

The two-photon process of microscopy provides good spatial resolution and optical sectioning ability when observing quasi-static endogenous fluorescent tissue within an in vivo animal model skin. In order to extend the use of such systems, we developed a two-photon laser scanning microscopy system capable of also capturing $512{\times}512$ pixel images at 90 frames per second. This was made possible by incorporating a 72 facet polygon mirror which was mounted on a 55 kRPM motor to enhance the fast-scan axis speed in the horizontal direction. Using the enhanced temporal resolution of our high-speed two-photon laser scanning microscope, we show that rapid processes, such as fluorescently labeled erythrocytes moving in mouse blood flow at up to 1.2 mm/s, can be achieved.

• 비파괴검사학회지
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• 제33권3호
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• pp.283-288
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• 2013

This paper describes development of a high speed Doppler OFDI system for non-invasive vascular imaging. Doppler OFDI (optical frequency domain imaging) is one of the phase-resolved second generation OCT (optical coherence tomography) techniques for high resolution imaging of moving elements in biological tissues. To achieve a phase-resolved imaging, two temporally separated measurements are required. In a conventional Doppler OCT, a pair of massively oversampled successive A-lines is used to minimize de-correlation noise at the expense of significant imaging speed reduction. To minimize a de-correlation noise between targeted two measurements without suffering from significant imaging speed reduction, several methods have been developed such as an optimized scanning pattern and polarization multiplexed dual beam scanning. This research represent novel imaging technique using frequency multiplexed dual beam illumination to measure exactly same position with aimed time interval. Developed system has been verified using a tissue phantom and mouse vessel imaging.

• Current Optics and Photonics
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• 제3권1호
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• pp.54-65
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• 2019

To develop a biomarker predicting tumor treatment efficacy is helpful to reduce time, medical expenditure, and efforts in oncology therapy. In clinics, microvessel density using immunohistochemistry has been proposed as an indicator that correlates with both tumor size and metastasis of cancer. In the preclinical study, we hypothesized that vascular morphometrics using optical coherence tomography angiography (OCTA) could be potential indicators to estimate the treatment efficacy of breast cancer. To verify this hypothesis, a 13762-MAT-B-III rat breast tumor was grown in a dorsal skinfold window chamber which was applied to a nude mouse, and the change in vascular morphology was longitudinally monitored during tumor growth and metronomic cyclophosphamide treatment. Based on the daily OCTA maximum intensity projection map, multiple vessel parameters (vessel skeleton density, vessel diameter index, fractal dimension, and lacunarity) were compared with the tumor size in no tumor, treated tumor, and untreated tumor cases. Although each case has only one animal, we found that the vessel skeleton density (VSD), vessel diameter index and fractal dimension (FD) tended to be positively correlated with tumor size while lacunarity showed a partially negative correlation. Moreover, we observed that the changes in the VSD and FD are prior to the morphological change of the tumor. This feasibility study would be helpful in evaluating the tumor vascular response to treatment in preclinical settings.

• 전자공학회논문지SC
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• 제49권1호
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• pp.12-19
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• 2012

국내외적으로 경제수준의 향상과 웰빙 트렌트로 인해 피부미용기기 시장이 지속적으로 성장하고 있다. 본 연구에서는 기존 대형기기 및 일부 보급된 개인용 피부미용기기와 차별화된 개인용 피부미용 복합자극기를 개발하고자 하였다. 복합자극의 종류로 한약재 추출액의 생화학 자극, 피부 신진대사를 촉진시키는 온열 자극, 다양한 피부미용 효과가 보고되고 있는 광 자극을 선택하였으며, 이들이 동시에 제공 가능하도록 복합자극부 구조를 설계하였다. 생화학 자극은 탈지면 패드에 함유된 한약재혼합추출물이 온열에 의해 기화됨으로써 피부에 공급되도록 하였으며, 온열자극은 탄소섬유 면상 발열체를 통해, 그리고 광자극은 850nm 파장을 갖는 고출력 근적외선 LED를 통해 공급되도록 하였다. 제작된 시제품의 보완 및 성능평가를 위하여 온열자극부 및 광 자극부를 테스트하였다. 온열자극의 경우 목표온도 도달시간, 제어 오차가 각 2분 이내, ${\pm}1.5^{\circ}C$ 이내로 적절히 동작하였으며, 광 자극의 경우 근적외선 LED와 발열체가 결합된 구조로 인해 정상 동작범위 이상으로 온도가 상승하는 것이 확인되어 방열체 제작 추가함으로써 이 문제를 해결하였다. 마지막으로, C2C12 mouse myoblast을 대상으로 한약재 자극을 제외한 복합자극의 유효성을 평가하는 실험을 실시하였으며, RT-PCR분석 결과 $37^{\circ}C$ 온열자극과 광 자극의 복합자극을 인가한 그룹에서 대조군에 비해 collagen I mRNA 발현량이 4.9배, collagen III mRNA 발현량이 1.3배 증가하는 것을 확인하였다.

• 한국정보통신학회논문지
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• 제20권3호
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• pp.639-646
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• 2016

본 연구에서는 특수한 상황(미끄러짐 발생 및 회전관성이 부하로 작용하는 경우)에서의 회전 엔코더 기반 측정방법의 단점을 극복하기 위하여 광 변위센서(ADNS-9500)를 사용한 비접촉식 변위 측정방법을 제안하였다. 정확한 변위 측정을 위해 데이터 수집 보드와 랩뷰를 활용하여 실험적으로 광 변위센서의 성능을 분석하였으며, 반복실험을 통해 실험조건(측정방향, 속도, 가속도, 높이, 표면 재질)에 따른 광 변위센서의 성능특성을 파악하였다. 성능실험 결과, 광 변위센서를 사용하여 정확하게 평면운동을 측정하기 위해서는 광 변위센서와 지면(대상 물체의 표면)과의 높이를 2.4 mm-3.2 mm로 일정하게 유지시켜야 하며, 각각의 축 방향, 속도, 표면 재질을 고려하여 민감도를 수정하여 변위 계산식에 적용해야 하는 것을 확인하였다.

• Archives of Plastic Surgery
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• 제35권6호
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• pp.755-758
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• 2008

Purpose: In order to determine the amount of wound healing, objective sequential assessments of changes in wound size and depth are essential. Although a variety of measurements for wound healing have been proposed, a gold standard for quantifying day-to-day changes in healing has not been established. We present here a simple and non-invasive wound measurement method that quantitatively and accurately documents changes of the size of a raw surface and the volume of a soft tissue defect using a stereoimage optical topometer(SOT) system. Methods: Using a 5mm diameter biopsy punch, four circular wounds were created on abdominal area of a diabetic mouse. Photographs were taken using SOT system at baseline, 5th day and 10th postoperative day. The wound margin was traced on a digitalized photo and evaluated the area and the volume of the wound by SOT system. Results: The SOT system calculated a mean wound surface of $15.93{\pm}0.29mm^2$ and volume of $827.50{\pm}88.86$ intensity/pixel${\times}$area(I/PA) immediately after wounding. On the 5th day after the operation wound surface declined by $10.73mm^2$ and on the 10th day declined by $5.95mm^2$. The wound volume also declined from 827.50 I/PA to 161.75 I/PA and 30.50 I/PA on 0, 5th and 10th day, respectively. Conclusion: The SOT system described in this study represents a reliable, simple, practical, and non-invasive technique to accurately monitor and evaluate wound healing.

• Nuclear Medicine and Molecular Imaging
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• 제43권4호
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• pp.344-351
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• 2009

목적: 광학영상기술은 소동물이나 임상연구에서 분자영상법으로 알려진 첨단연구 분야이다. 광학영상기기는 소동물영상연구 및 추적연구에 중요한 역할을 수행하고 있다. 발광영상에서 소동물을 영상화 하기 위해서는 피부조직을 뚫고 나오는 광자를 검출하기 위한 고민감도 CCD카메라가 필요하다. 이 연구에서는 소동물에서 발생하는 발광신호를 검출하기 위해 개발한 광학영상기기를 소개하고자 한다. 대상 및 방법: 냉각형 CCD카메라와 집광렌즈, 8개의 백색광 LED광원을 암실상자 안에 장치하였다. 팬텀 및 튜브를 이용한 영상을 얻은 후 발광 박테리아를 이용하여 CT26 암모델 누드마우스에서 영상을 획득하였다. 결과: 발광영상을 얻기 위한 광학영상기기를 설계하고 개발하였다. 영상획득이 성공적으로 수행되었고, 시스템을 완성하였다. 개발된 장비는 분자영상연구에 사용되고 있다. 결론 개발된 광학영상장비는 다양한 실험적 조건을 만족하는 연구에 최적화하여 유용한 도구로 자리잡을 것으로 기대한다.