• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.4
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• pp.287-292
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• 2009

Human skin is constantly exposed to environmental irritants such as smoke, chemicals and ultraviolet (UV). Free radicals and reactive oxygen species (ROS) caused by these environmental irritants play critical roles in cellular damage. In this study, to investigate the skin cell protective effect of Ophioglossum vulgatum extract, we investigated its effects on intercellular antioxidative activity and UVA-induced MMP expression in human dermal fibroblasts (HDFs). The dried O. vulgatum was extracted in a mixture of ethanol and water (1 : 1) for 24 h at room temperature. The extract was filtered and concentrated in vacuo and lyophilized. For testing intracellular ROS scavenging activity the cultured HDFs were analyzed by increase in DCF fluorescence upon exposure to UVB $20\;mJ/cm^2$. After treatment of O. vulgatum extracts, intracellular ROS levels were measured by luminescence spectrophotometer. Enzyme linked immuno sorbent assay (ELISA), and RT-PCR techniques were used for evaluating the effects of O. vulgatumon on MMP protein and mRNA expression in UVA irradiated HDFs. O. vulgatum extract was found to have ROS scavenging activity with the $IC_{50}$ values of $18.2\;{\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system. After treatment of O. vulgatum extracts, the oxidation of CM-DCFDA was inhibited effectively and O. vulgatum extracts showed a potent free radical scavenging activity by 30.4 % at $100\;{\mu}g/mL$ in UVB-irradiated HDFs. UVA induced MMP protein expression was reduced 37.7 % by treatment with O. vulgatum extract, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Taken together, these results suggest that O. vulgatum extract prevents the skin cell damage induced by UV irradiation, and implies that O. vulgatum extract may be useful as a new ingredient for anti-aging cosmetics.

• Journal of Plant Biology
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• v.36 no.4
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• pp.337-343
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• 1993

Tracheid structure of aerial system in five species of Ophioglossaceae and one species of Osmundaceae was examined with light and scanning electron microscopy. The species were Botrichium multifidum var. robustrum, B. ternatum, strictum, B. virginianum, Ophioglossum vulgatum, and Osmunda japonica. Three types of tracheids could be recognized by the pattern of secondary wall thickening; helical, irregular reticulate and circular bordered pitted tracheids. Among them, the appearance of circular bordered pitted tracheids supported that ophioglossaceous plants might have phylogenetic relationship with progymnosperm-seed plant line. Circular and elliptical bordered pits in shape were observed in five ophioglossaceous species; the former was subdivided into large and small types in size. In conclusion, three types of bordered pits were found: (1) large circular type- O. vulgatum, (2) small circular type- B. multifidum var. robustrum, B. ternatum and (3) elliptical type-B. strictum, B. virginianum. B. strictum and B. virginianum belong to subgenus Osmundopteris had the elliptical bordered pits. Therefore, this group might be interpreted as the most primitive in this family; however, such suggestion did subgenus Osmundopteris was classified into the most advanced group.

• Journal of Species Research
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• v.2 no.2
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• pp.227-244
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• 2013

We investigated the flora of Jang-do (Isl.), an island of the Heuksan archipelago, located in Heuksan-myeon, Shinan-gun, Jeollanam-do and discussed the remarkable plants found there. Thirteen-field trips (total 27 days) were completed from May of 2004 to May of 2013. As a result, we identified the distribution of 423 taxa in Jang-do (Isl.), comprising 104 families, 285 genera, 335 species, 78 varieties and 10 forms. There are a total of 95 taxa of floristic regional indicator plants. The floristic regional indicators are as follows; four taxa of level V such as Impatiens furcillata, four taxa of level IV such as Hosta yingeri, 14 taxa of level III such as Ophioglossum vulgatum, 5 taxa of level II, and 58 taxa of level I. In addition 10 red data plants, 2 vulnerable species (VU) including Calanthe striata for. sieboldii, and 4 least concern species (LC) like Ardisia crenata, 2 not evaluated species (NE) such as Hosta yingeri, were found in the investigated area. An unrecorded species, Fragaria (Rosaceae), was found in a forest of Jang-do (Isl.) for the first time in Korea.