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Genetic Structure of xyl Gene Cluster Responsible for Complete Degradation of (4-Chloro )Benzoate from Pseudomonas sp. S-47

  • Park, Dong-Woo;Lee, Kyoung;Chae, Jong-Chan;Kudo, Toshiaki;Kim, Chi-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.483-489
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    • 2004
  • Pseudomonas sp. S-47 is a bacterium capable of degrading benzoate as well as 4-chlorobenzoate (4CBA). Benzoate and 4CBA are known to be degraded via a meta-cleavage pathway characterized by a series of enzymes encoded by xyl genes. The meta-cleavage pathway operon in Pseudomonas sp. S-47 encodes a set of enzymes which transform benzoate and 4CBA into TCA cycle intermediates via the meta-cleavage of (4-chloro )catechol to produce pyruvate and acetyl-CoA. In the current study, the meta-pathway gene cluster was cloned from the chromosomal DNA of S-47 strain to obtain pCS1, which included the degradation activities for 4CBA and catechol. The genetic organization of the operon was then examined by cloning the meta-pathway genes into a pBluescript SKII(+) vector. As such, the meta-pathway operon from Pseudomonas sp. S-47 was found to contain 13 genes in the order of xylXYZLTEGFlQKIH. The two regulatory genes, xylS and xylR, that control the expression of the meta-pathway operon, were located adjacently downstream of the meta-pathway operon. The xyl genes from strain S-47 exhibited a high nucleoside sequence homology to those from Pseudomonas putida mt-2, except for the xylJQK genes, which were more homologous to the corresponding three genes from P. stutzeri AN10. One open reading frame was found between the xylH and xylS genes, which may playa role of a transposase. Accordingly, the current results suggest that the xyl gene cluster in Pseudomonas sp. S-47 responsible for the complete degradation of benzoate was recombined with the corresponding genes from P. putida mt-2 and P. stutzeri AN10.

The Effects of the Hot, Humid Tropical Climate and Early Age Feed Restriction on Stress and Fear Responses, and Performance in Broiler Chickens

  • Al-Aqil, A.;Zulkifli, I.;Sazili, A.Q.;Omar, A.R.;Rajion, M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.11
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    • pp.1581-1586
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    • 2009
  • The present study was conducted to determine the effects of two types of housing systems and early age feed restriction on stress and fear reactions, and performance in broiler chickens raised in a hot, humid tropical climate. On day 1, chicks were housed either in windowless environmentally controlled chambers (temperature was set at 32$^{\circ}C$ on day 1 and gradually reduced to 23$^{\circ}C$ by day 21) or in conventional open-sided houses (OH) with cyclic temperatures (minimum, 24$^{\circ}C$; maximum, 34$^{\circ}C$). An equal number of chicks from each housing system was subjected to either ad libitum feeding (AL) or 60% feed restriction on day 4, 5 and 6 (FR). The CH birds showed greater weight gain, higher feed consumption and better feed conversion ratios (FCR) than their OH counterparts. Feeding regimen had negligible effect on overall performance. Neither housing nor feeding regimen had a significant (p<0.05) effect on mortality rate. Although the CH birds were less stressed, as measured by plasma corticosterone concentration (CORT), than those of OH, the former showed longer TI duration suggesting higher magnitude of underlying fearfulness. A significant (p<0.05) effect of housing on heterophil/lymphocyte ratios was only noted among the AL birds where the CH birds had higher values than OH. Collectively, these results suggest that although OH birds had poorer performance and higher level of stress than CH, the former were less fearful. Although FR had negligible effect on growth performance, the regimen alleviated both stress and fear reactions in broilers.

Empirical Exploration on the Component Factors of Creativity·Character for College Students (대학생 창의·인성 구성요인에 관한 경험적 탐색)

  • Lee, Ji-Sun
    • The Journal of the Korea Contents Association
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    • v.17 no.2
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    • pp.26-37
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    • 2017
  • This study aims to theoretically and empirically establish the characteristics and component factors of creativity character of college students. Putting together preceding researches related to the concept and structure of creativity character through the theoretical approach, the in-depth interview and open survey were conducted targeting 70 specialists in education, education engineering, consultation meeting, and researchers' meeting about creativity character of college students. In the results, as creativity character for college students, creativity was classified into cognitive creativity and affective creativity while character was classified into moral character, social character, and emotional character. Especially, the creativity character of college students emphasized the importance of character more than creativity. Through such results, it aims to suggest a new perspective on creativity character of college students, and moreover to provide basic data to set up the research direction to develop a tool to measure creativity character of college students for follow-up researches. Also, it is considered to establish the theoretical, empirical foundation contributing to the establishment of teaching-learning method and education engineering qualitatively enhanced in the site of college.

An Analysis of Transshipment Competitiveness of Container Cargoes in Incheon New Port (인천신항의 환적경쟁력 분석에 대한 연구)

  • Ahn, Woo-Chul;Yeo, Gi-Tae;Yang, Chang-Ho
    • Journal of Korea Port Economic Association
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    • v.26 no.1
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    • pp.20-42
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    • 2010
  • This study is aimed at evaluating transshipment competitiveness of Incheon New Port which will open in 2013. For this reason, we used Conjoint Analysis(CA) for a methodology of this research as CA had been frequently adopted for empirical analysis of new container terminal in previous studies. We have provided the questionnaires to the stake holders of the port such as experts in Port Authority(PA), logistics companies, and terminal operators. The result showed that transshipment competitiveness of Incheon New Port was subordinate to transshipment costs and port costs of Busan and Shanghai. Overall, we hope this study could help draw up the policies on a New Port that will attract transshipment cargoes and set up marketing plan for an early settlement.

Anomaly Detection in Sensor Data

  • Kim, Jong-Min;Baik, Jaiwook
    • Journal of Applied Reliability
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    • v.18 no.1
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    • pp.20-32
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    • 2018
  • Purpose: The purpose of this study is to set up an anomaly detection criteria for sensor data coming from a motorcycle. Methods: Five sensor values for accelerator pedal, engine rpm, transmission rpm, gear and speed are obtained every 0.02 second from a motorcycle. Exploratory data analysis is used to find any pattern in the data. Traditional process control methods such as X control chart and time series models are fitted to find any anomaly behavior in the data. Finally unsupervised learning algorithm such as k-means clustering is used to find any anomaly spot in the sensor data. Results: According to exploratory data analysis, the distribution of accelerator pedal sensor values is very much skewed to the left. The motorcycle seemed to have been driven in a city at speed less than 45 kilometers per hour. Traditional process control charts such as X control chart fail due to severe autocorrelation in each sensor data. However, ARIMA model found three abnormal points where they are beyond 2 sigma limits in the control chart. We applied a copula based Markov chain to perform statistical process control for correlated observations. Copula based Markov model found anomaly behavior in the similar places as ARIMA model. In an unsupervised learning algorithm, large sensor values get subdivided into two, three, and four disjoint regions. So extreme sensor values are the ones that need to be tracked down for any sign of anomaly behavior in the sensor values. Conclusion: Exploratory data analysis is useful to find any pattern in the sensor data. Process control chart using ARIMA and Joe's copula based Markov model also give warnings near similar places in the data. Unsupervised learning algorithm shows us that the extreme sensor values are the ones that need to be tracked down for any sign of anomaly behavior.

Uncertainty for Privacy and 2-Dimensional Range Query Distortion

  • Sioutas, Spyros;Magkos, Emmanouil;Karydis, Ioannis;Verykios, Vassilios S.
    • Journal of Computing Science and Engineering
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    • v.5 no.3
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    • pp.210-222
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    • 2011
  • In this work, we study the problem of privacy-preservation data publishing in moving objects databases. In particular, the trajectory of a mobile user in a plane is no longer a polyline in a two-dimensional space, instead it is a two-dimensional surface of fixed width $2A_{min}$, where $A_{min}$ defines the semi-diameter of the minimum spatial circular extent that must replace the real location of the mobile user on the XY-plane, in the anonymized (kNN) request. The desired anonymity is not achieved and the entire system becomes vulnerable to attackers, since a malicious attacker can observe that during the time, many of the neighbors' ids change, except for a small number of users. Thus, we reinforce the privacy model by clustering the mobile users according to their motion patterns in (u, ${\theta}$) plane, where u and ${\theta}$ define the velocity measure and the motion direction (angle) respectively. In this case, the anonymized (kNN) request looks up neighbors, who belong to the same cluster with the mobile requester in (u, ${\theta}$) space: Thus, we know that the trajectory of the k-anonymous mobile user is within this surface, but we do not know exactly where. We transform the surface's boundary poly-lines to dual points and we focus on the information distortion introduced by this space translation. We develop a set of efficient spatiotemporal access methods and we experimentally measure the impact of information distortion by comparing the performance results of the same spatiotemporal range queries executed on the original database and on the anonymized one.

A Study on Measurement and Improvement of Service Quality using QFD in the Law Firm Industry (QFD를 이용한 기업 법무 서비스 품질 측정 및 개선에 관한 연구)

  • Pyo, In Soo;Lee, Jae Kwang
    • Knowledge Management Research
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    • v.17 no.2
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    • pp.1-26
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    • 2016
  • As entry barriers at the legal service market in Korea have been recently lifted, the legal service market has dramatically changed from a seller's market to a buyer's market. Externally, the Korean legal service market has been open to foreign law firms and internally, lawyers graduating from law schools have been increasing. In addition, as even the general public can easily access, through the official sites of government organizations, to the basic legal information such as laws and judicial precedents, the degree of information asymmetry has also decreased. These phenomena require the legal service industry to be a more client-oriented and more quality-oriented one. Having said that, legal service providers also need to continue to make strenuous efforts to measure and improve the service quality from the customers' perspective. Under these circumstances, this study introduced a certain type of model which may be of help of measuring and improving the quality of legal service of law firms, in particular, with a focus on those firms providing corporate legal service. This study used a Quality Function Deployment (QFD) in order to measure and thereby improve the quality of corporate legal service in the law firm industry. Above all, this study showed, by using SERVQUAL, each and every service attributes of quality measurement of the corporate legal service and also calculated the degree of importance of service quality at each process of providing corporate legal service. Next, this study introduced all supporting factors necessary for the provision of corporate legal service and calculated the degree of importance of each and every supporting factors in the service process. This study may be of help to provide the substantial and systematic approaches to the corporate legal service providers who would like to set up a certain methods of measuring and further improving the quality of corporate legal service from the customers' perspective.

A Program Analysis Technique for Recovery of Layered Architecture and Conformance Checking (층위구조 아키텍처의 복구 및 일치성 검사를 위한 프로그램 분석 방법)

  • Park Chanjin;Hong Euyseok;Kang Yoohoon;Wu Chisu
    • Journal of KIISE:Software and Applications
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    • v.32 no.8
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    • pp.730-741
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    • 2005
  • Layered Architecture is a kind of nodule decomposition techniques, which decomposes a program by generality This paper proposes a ]aver based method for recovering layered architecture from object-oriented program and checking conformance against architectural document. To specify the rules for layered style in object-oriented program, we define a partially ordered set on modules by module use relationship and module layer relationship by module override relationship. The meaning of module layer relationship is explained with an example from design patterns. Steps to recover layered architecture from program are described and a metamodel for the recovery is proposed. Architecture recovery is performed on source codes from open-source software project, and the implication of parts that do not conform to its architectural document is discussed. As a result of checking, it is pointed out that, although the parts are considered allowable exceptions of layered architecture, their modifications should be controlled carefully.

A Method for Selective Storing and Visualization of Public Big Data Using XML Structure (XML구조를 이용한 공공 빅데이터의 선별 저장 및 시각화 방법)

  • Back, BongHyun;Ha, Il-Kyu
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.12
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    • pp.2305-2311
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    • 2017
  • In recent years, there have been tries to open public data from various government agencies along with publicization of public information for the public interest. In other words, various kinds of electronic data generated and collected by the public institutions as a result of their work are opened in the public portal sites. However, users who use it are limited in their use of big data due to lack of understanding of data format, lack of data processing knowledge, difficulty in accessing and managing data, and lack of visualization data to understand collected and stored data. Therefore, in this study, we propose a big data collection, storing and visualization platform that can collect big data provided by various public sites using data set URL and API regardless of data format, re-process collected data using XML structure.

A Study of Over Voltage Ground Relay Operation Status at Opening of No-load Charged Cable (무부하 충전케이블 개방시 잔류전압에의한 과전압계전기 동작현상 연구)

  • Kim, Yeong-Han;Choi, Jong-Hyuck;Yoon, Ki-Seob
    • Proceedings of the KIEE Conference
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    • 2000.07a
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    • pp.185-187
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    • 2000
  • Fault current is flowed into 154/23kV M. Tr when line-to-ground fault occurs in power system. NGR(Neutral Grounded Reactor) is set up in order to prevent M.Tr fault by limiting magnitude of fault currents. Here, disconnection of NGR causes voltage increase by L-C resonance and line-to-ground fault in an unearthed system results in voltage increase at healthy phases. So Over Voltage Ground Relay(OVGR) is used for tripping M.Tr. Also, buses at second phases of M.Trs are all connected with section circuit breakers closed for the purpose of parallel operation and load shedding. In case of speciality buses are comprised of power cable in part for GIS connection. When no-load charged cable or bus is open by a section CB, unbalanced voltage charged on the bus is induced. Also discrepant opening time for circuit breakers on different phases gives rise to unbalanced zero sequence voltage. It was observed that this zero sequence voltage detected in the 22.9kV P.T (Potential Transformer for bus) mal-operated 59GT and tripped M.Tr. The zero sequence voltage of which vanishing time is longer than relay operating time came out by EMTDC simulation. Also, it was shown that the voltage waves of actual test are similar to those of simulation. On the basis of above results, R-C circuit complement on the relay without any effect on a power system made operating time of the relay longer than vanishing time of distorted waves. Consequently, operating time of the relay was delayed and magnitude of distorted waves was decreased by increasing time constant of the relay.

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