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Nucleotide sequence analysis of a second set of the polyketide synthase .betha.-ketoacyl synthase and chain length factor genes from the salinomycin-producing streptomyces albus

  • Hyun, Chang-Gu;Park, Kwan-Hyung;C.Richard Hutchinson;Suh, Joo-Won
    • Journal of Microbiology
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    • v.35 no.1
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    • pp.40-46
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    • 1997
  • The pWHM220 cosmid with a 24-kb insert cloned from Streptomyces albus ATCC 21838 induces the biosynthesis of a polysther antibiotic similar to salinomycin in Streptomyces invidans. We have analyzed this region by DNA sequencing as well as Southern blot hybridization with type I and type II polyketide synthase (PKS) probes. Surprisingly, we found another set of type II SKS genes only 10-kb from the original PKS genes, salABCDE. The DNA sequence revealed two complete open reading frames (ORFs) named salB2 and salC2, and one partial ORF that does not resemble any known DNA or deduced protein sequence. The salC2 should code for chain length determining factor while the deduced amino acid sequence encoded by salB2 exhibits high similarity to .betha.-ketoacyl synthase from different PKS gene clusters. The highest identity was found for .betha.-keetoacyl synthases from S. argillaceus (MtmP. 59.1% identity), the mithramycin producer and from S. venezuelae ISP5230 (JadA, 52.3% identity), the jadomycin producer. The SalC2 protein clearly resembles its counterparts in order aromatic PKS gene clusters that are believed to influence the length of the polyketide chain. The highest identities observed were to that of S. argillaceus (MtmK, 62.3%) and S. venezuelae ISP 5230 (JadB, 55.1%) proteins, Moreover, the deduced amino acid sequences of the salB2 and salC2 products were 29.0% identical.

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A Study on the Publicness of Artistic Creative Space - Focused on Seoul Art Space - (예술창작공간의 공공성에 관한 연구 - 서울시창작공간의 사례를 중심으로 -)

  • Cho, Youn-Joo;Shin, Kyung-Joo
    • Korean Institute of Interior Design Journal
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    • v.21 no.6
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    • pp.35-45
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    • 2012
  • As the importance of economic value of culture has increased, governments have set a strategy to secure national competitiveness through cultural art. One of concrete ways is establishing a cultural space by using idle facilities. This study aims to analyze publicness of Seoul Art Space, suggest ways to improve the facilities of creative space and provide basic date for the further plan of creative space. The object of this study is 8 facilities from Seoul Art Space which is operated by City of Seoul. Through literature review, we extracted 5 elements(each element has 4 sub categories) for publicness(accessibility, openness, convenience, historical connectivity and stay time) and to evaluates publicness of the Seoul Art Space, we analyzed these facilities based on these elements. The results are as follows: The publicness of Seoul Art Space acquired 96.9%(100% is 64 points) for stay time, 95.3% for convenience, 90.6% for accessibility, 73.4% for openness and 62.5% for historical connectivity. For the openness, we suggest to use glass materials and open the space to the public. To improve historical connectivity, we suggest to determine appropriate theme for a particular space or area and set a function of buildings for the differentiation.

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Prediction of Tidal Changes due to the Development of Incheon Coastal Waters (인천해역 개발에 따른 조석변화 추정)

  • 정신택;소재귀;채장원
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.6 no.3
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    • pp.266-274
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    • 1994
  • Two-dimensional numerical analysis is performed for the simulation of tidal characteristics related to various development projects in Incheon coastal waters along the west coast of Korea. Field observation of tides and currents was made in order to provide the input boundary and validation data set to the numerical modelling. For the simulation of changes of tides and currents a depth-integrated two-dimensional shallow water model of Flather and Heaps (1975) has been used herein. Tidal model is set up with open boundary sea level from observed two major constituents, M$_2$ and S$_2$. Subsequently the established model is utilized to investigate the effect of two development projects in this region. It has been found that in spring tide the changes of tidal amplitude are small, however, those of tidal current are locally significant.

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Tree-Pattern-Based Clone Detection with High Precision and Recall

  • Lee, Hyo-Sub;Choi, Myung-Ryul;Doh, Kyung-Goo
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.5
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    • pp.1932-1950
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    • 2018
  • The paper proposes a code-clone detection method that gives the highest possible precision and recall, without giving much attention to efficiency and scalability. The goal is to automatically create a reliable reference corpus that can be used as a basis for evaluating the precision and recall of clone detection tools. The algorithm takes an abstract-syntax-tree representation of source code and thoroughly examines every possible pair of all duplicate tree patterns in the tree, while avoiding unnecessary and duplicated comparisons wherever possible. The largest possible duplicate patterns are then collected in the set of pattern clusters that are used to identify code clones. The method is implemented and evaluated for a standard set of open-source Java applications. The experimental result shows very high precision and recall. False-negative clones missed by our method are all non-contiguous clones. Finally, the concept of neighbor patterns, which can be used to improve recall by detecting non-contiguous clones and intertwined clones, is proposed.

Investigation into shear properties of medium strength reinforced concrete beams

  • Shah, Attaullah;Ahmad, Saeed;Khan, Salimullah
    • Structural Engineering and Mechanics
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    • v.35 no.3
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    • pp.265-282
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    • 2010
  • The shear contribution of transverse steel in reinforced concrete beams is generally assumed as independent of the concrete strength by most of the building codes. The shear strength of RC beams with web reinforcement is worked out by adding the individual contributions of concrete and stirrups. In this research 70 beams of medium strength concrete in the range of 52-54 MPa, compressive strength were tested in two sets of 35 beams each. In one set of 35 beams no web reinforcement was used, whereas in second set of 35 beams web reinforcement was used to check the contribution of stirrups. The values have also been compared with the provisions of ACI, Eurocode and Japanese Code building codes. The results of two sets of beams, when compared mutually and provisions of the building codes, showed that the shear strength of beams has been increased with the addition of stirrups for all the beams, but the increase is non uniform and irregular. The comparison of observed values with the provisions of selected codes has shown that EC-02 is relatively less conservative for low values of longitudinal steel, whereas ACI-318 overestimates the shear strength of RC beams at higher values of longitudinal steel. The Japanese code of JSCE has given relatively good results for the beams studied.

Effects of Balance Training through Visual Control on Balance Ability, Postural Control, and Balance Confidence in Chronic Stroke Patients (시각 통제를 이용한 균형훈련이 만성 뇌졸중 환자의 균형능력과 자세조절, 균형자신감에 미치는 영향)

  • Jeong, Seong-Hwa;Koo, Hyun-Mo
    • PNF and Movement
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    • v.18 no.1
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    • pp.133-141
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    • 2020
  • Purpose: The purpose of this study was to conduct balance training through vision control to improve the balance, postural control, and balance confidence and to decrease the visual and sensory dependence of stroke patients. Methods: Twenty-eight chronic stroke patients volunteered to participate in the study. They were randomly assigned to the eyes-closed and the eyes-open training groups. Three times a week for four weeks each group performed an unstable-support session and a balance training session for thirty minutes per set. Their balance, postural control, and balance confidence were assessed using BIO Rescue (BR), the postural assessment scale for stroke (PASS), and the Korean activity-specific balance confidence scale (K-ABC), respectively. All data were analyzed using SPSS version 22.0. Statistical methods before and after working around the average value of each dataset were independent T-test. The significance level for statistical analyses was set at 0.05. Results: Comparison between the groups showed statistically significant effects on all variables before and after the intervention (p < 0.05). Conclusion: This study reflected that balance-training programs involving vision control improve the balance, postural control, and balance confidence of chronic stroke patients. Thus, stroke patients should undergo training programs that increase the use of their other senses with vision control in clinical practice.

The Method of Abandoned Object Recognition based on Neural Networks (신경망 기반의 유기된 물체 인식 방법)

  • Ryu, Dong-Gyun;Lee, Jae-Heung
    • Journal of IKEEE
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    • v.22 no.4
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    • pp.1131-1139
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    • 2018
  • This paper proposes a method of recognition abandoned objects using convolutional neural networks. The method first detects an area for an abandoned object in image and, if there is a detected area, applies convolutional neural networks to that area to recognize which object is represented. Experiments were conducted through an application system that detects illegal trash dumping. The experiments result showed the area of abandoned object was detected efficiently. The detected areas enter the input of convolutional neural networks and are classified into whether it is a trash or not. To do this, I trained convolutional neural networks with my own trash dataset and open database. As a training result, I achieved high accuracy for the test set not included in the training set.

Characteristics of Water Surface Variation around Double-Breaking Type Artificial Reef (월류형 잠제 주위의 수면 변동 특성)

  • Shin, Young-Seop;Lee, Seong-Dae
    • Journal of Ocean Engineering and Technology
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    • v.33 no.3
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    • pp.280-288
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    • 2019
  • A submerged breakwater is one of the coastal structures used to reduce wave energy and coastal erosion. However, a submerged breakwater has a negative aspect in that a strong rip current occurring around an open inlet due to a difference in mean water levels at the front and rear sides of the structure leads to scouring. Such scouring has a bad effect on its stability. In order to eliminate this kind of demerit, this study investigated an artificial reef of the overflow type with openings. We also developed a program where the flows around the artificial reef of the overflow type could be analyzed numerically. An unstructured grid system was used to cover the various geometries, and the level set method was applied to treat the movement of the free surface. To verify these numerical schemes, hydraulic physical tests were performed on the submerged breakwater and double breaking type artificial reef. Then, the wave height and velocity distribution around the reef were examined using the experimental results. Comparisons between the results of hydraulic and numerical tests showed reasonable agreement.

PCRM: Increasing POI Recommendation Accuracy in Location-Based Social Networks

  • Liu, Lianggui;Li, Wei;Wang, Lingmin;Jia, Huiling
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.11
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    • pp.5344-5356
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    • 2018
  • Nowadays with the help of Location-Based Social Networks (LBSNs), users of Point-of-Interest (POI) recommendation service in LBSNs are able to publish their geo-tagged information and physical locations in the form of sign-ups and share their experiences with friends on POI, which can help users to explore new areas and discover new points-of-interest, and promote advertisers to push mobile ads to target users. POI recommendation service in LBSNs is attracting more and more attention from all over the world. Due to the sparsity of users' activity history data set and the aggregation characteristics of sign-in area, conventional recommendation algorithms usually suffer from low accuracy. To address this problem, this paper proposes a new recommendation algorithm based on a novel Preference-Content-Region Model (PCRM). In this new algorithm, three kinds of information, that is, user's preferences, content of the Point-of-Interest and region of the user's activity are considered, helping users obtain ideal recommendation service everywhere. We demonstrate that our algorithm is more effective than existing algorithms through extensive experiments based on an open Eventbrite data set.

Technique and Implementation of Secure Downloadable Conditional Access System (안전한 다운로드 가능 제한 수신 시스템 제안 및 구현)

  • Kang, Seong-Ku;Park, Jong-Youl;Paik, Eui-Hyun;Park, Choon-Sik;Ryou, Jae-Cheol
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.19 no.6
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    • pp.161-174
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    • 2009
  • IPTV provides their services only for their subscribers who have a eligibility to watch it by using Conditional Access System(CAS). CAS has been servicing their contents for subscribers by using set-top box or cable card so far, but these days, to solve the compatibility between kinds of devices, linkage with other services as DRM and confirming stability, the research of Downloadable CAS(DCAS) is being advanced steadily. On this paper, we analyse the vulnerability of DCAS based on the OpenCable and make up for the vulnerability in DCAS, then proposes to use secure DCAS system for IPTV. Also we show the result of the research and analyse the satisfaction of requirements.