• 한국식품과학회지
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• 제4권4호
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• pp.317-321
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• 1972

왜문어 Octopus vulgaris의 천일건조 및 건조 후 3개월간 저장 중의 핵산관련물질의 변화를 이온교환수지 column chromatography 법으로 실험하여 다음과 같은 결과를 얻었다. 살아있는 왜문어에서 절단한 생시료(다리) 중에는 inosine이 $9.4\;{\mu}mole/g{\cdot}dry\;wt.$로서 가장 함량이 많았고, 어류와는 달리 IMP는 존재하지 않았다. 건조중 ATP, ADT 및 inosine은 감소하고, AMP 및 hypoxanthine은 증가하였다. 특히 inosine은 생시료때 $9.4\;{\mu}mole/g\;dry\;wt.$이던것이 $3.0\;{\mu}mole/g\;dry\;wt.$로 감소하는 반면, hypoxanthine은 $5.1\;{\mu}mole/g\;dry\;wt.$에서 $17.6\;{\mu}mole/g\;dry\;wt.$로 증가하였다. 건조후 3개월간 저장 중에는 ADP, AMP 및 inosine은 감소하고 hypoxanthine은 약 2배로 증가하였다.

• 한국잠사곤충학회지
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• 제45권2호
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• pp.90-95
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• 2003

최근 천연색소의 다양한 생리 활성이 보고됨에 따라 오디에 함유된 C3G 색소에 대해서도 관심이 높아지고 있다. 이에 오디로부터 C3G를 분리$.$정제하고 정제된 C3G와 표준물질의 분자량을 비교ㆍ동정하였다. 또한 유전자원으로 보존되고 있는 뽕 품종 및 계통에 대한 C3G 함량을 분석하여 고함유 계통을 선발하였다. 1. Amberlite IRC-50 ion exchange resin이 충진된 유리 column을 산성화시킨 후 이온교환수로 비흡착색소, 수용성 당류, 유기산 및 아미노산 등을 제거하였으며, column에 흡착된 색소를 용출 및 감압농축하여 분말상태로 정 제된 색소물질 C3G(cyanidin-3-glucoside)를 얻었다. 2. Waters 996 Photodiods Array Detector를 이용하여 정제된 C3G(cyanidin-3-glucoside)의 흡수 spectrum을 조사한 결과, 각각 516nm과 280nm에서 최대흡수 spectrum을 보임으로써 anthocyanin계 색소의 특이적 흡수 파장 영역인 500-550nm(Band I)과 280nm전후(Band II)와 일치하여 open column에 의해 분리된 색소가 anthocyanin 계열의 색소임을 확인하였다. 3. LC-Mass(Waters 2690 alliance Separation Module)를 사용하여 분자량을 확인한 결과, 449로서 C3G 표준물질의 분자량과 일치하였다. 4. 유전자원으로 보존되고 있는 뽕 품종 및 계통에 대한 C3G 함량을 분석한 결과, 공시한 35계통의 C3G 평균 함량은 0.9%였으며 계통간 함량의 차이가 있는 것으로 나타났다. 5. '수성뽕'. '강선' 및 '절곡조생(충북)'은 C3G함량은 물론 수량, 과중 및 당도에 대한 과실적 특성조사에서 양호한 성적을 나타내어 오디생산용 유망 계통으로 선발하였다.

• Natural Product Sciences
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• 제10권2호
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• pp.89-91
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• 2004

Six components were isolated from the $CH_2Cl_2$ fraction of Belamcanda chinensis rhizome by open column chromatography. Their structures were elucidated as ${\beta}-sitosterol$ (1), apocynin (2), decursin (3), iristectorigenin A (4), irigenin (5) and tectorigenin (6) by spectral analysis. Among these compounds, decursin (3) was isolated for the first time from a plant of the family Iridaceae.

• Natural Product Sciences
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• 제19권2호
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• pp.137-144
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• 2013

Phytochemical constituents were isolated from the seeds of tartary buckwheat (Fagopyrum tataricum) by open column chromatography. Their structures were elucidated as ${\beta}$-sitosterol (1), ${\beta}$-sitosterol-3-O-glucoside (2), oleanolic acid (3), kaempferol (4), quercetin (5), kaempferol-3-O-rutinoside (6), and quercetin-3-O-rutinoside (7) on the basis of spectroscopic analysis including $^1H$-, $^{13}C$-NMR, and MS. To our knowledge, oleanolic acid (3) has been isolated for the first time from the seeds of Fagopyrum species. The total contents of compounds 4 - 7 were 0.500 mg/g in Daesan maemil, 0.312 mg/g in Yangjul maemil, and 2.185 mg/g in tartary buckwheat.

• Archives of Pharmacal Research
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• 제25권3호
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• pp.285-288
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• 2002

Four compounds of a coumarin, a steroid and two flavonoids were isolated from the herba of Artemisia apiacea by open column chromatography. Their structures were elucidated as artemicapin C, apigenin, daucosterol and cacticin by chemical and spectroscopic analysis. This is the first report of the isolation of these compounds from this plant.

• Archives of Pharmacal Research
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• 제26권11호
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• pp.902-905
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• 2003

Five compounds of terpenoids and coumarins were isolated from the non-polar fraction of Artemisia apiacea by open column chromatography. Their structures were elucidated as $\alpha$-amyrin (1), $\beta$-amyrin (2), $\beta$-sitosterol (3), 5,6,7-trimethoxycoumarin (4) and 6-methoxy-7,8-methylenedioxycoumarin (5) by chemical and spectroscopic analysis. This is the first report of the isolation of $\alpha$-amyrin, $\beta$-amyrin, 5,6,7-trimethoxycoumarin and 6-methoxy-7,8-methylene-dioxycoumarin from this plant.

• Natural Product Sciences
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• 제20권3호
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• pp.206-210
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• 2014

Phytochemical constituents were isolated from Pinus koraiensis needles by open column chromatography. The structures isolated from the ethyl acetate fraction of P. koraiensis needles were elucidated as afzelin (1) and quercitrin (2) on the basis of spectroscopic analyses including $^1H$-NMR and MS. The amounts of afzelin (1) and quercitrin (2) in genus Pinus were determined by HPLC/UV analysis. Afzelin (1) and quercitrin (2) contents were highest in P. thunbergii needles. Because P. thunbergii needles contain high amounts of afzelin (1) and quercitrin (2), they have promising potential as new additives to natural medicinal products, health supplements, and beverages.

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.270-274
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• 2007

Two acetylmartynosides, 2"-acetylmartynoside and 3"-acetylmartynoside were isolated from the $CH_2Cl_2$ fraction of Clerodendron trichotomum (Verbenaceae) by open column chromatography and MPLC. Their chemical structures were identified by the direct comparison of spectral data reported in the literature, and their antioxidant properties were evaluated. Both compounds showed antioxidant activity based on evaluations of intracellular ROS and DPPH radical scavenging activities.

• 한국약용작물학회지
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• 제11권4호
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• pp.279-283
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• 2003

Five lignans were isolated from the chloroform fraction of Acanthopanax chiisanensis Nakai root by open column chromatography. Their structures were elucidated as (-)-sesamin (1), helioxanthin (2), savinin (3), taiwanin C (4), and 3-(3",4"-dimethoxybenzyl)-2-(3',4'-methylenedioxybenzyl) butyrolactone (5) by spectral analysis. Among them, compounds 2, 3, 4, and 5 were isolated for the first time from this plant.

• 한국환경과학회지
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• 제12권10호
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• pp.1117-1120
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• 2003

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD. Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.