• Journal of Veterinary Science
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• v.23 no.1
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• pp.1.1-1.11
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• 2022

Background: The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis. Objectives: This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain. Methods: Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers). The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically. Results: The optimized formulation was comprised of 50 mg/mL (COS), 32 mg/mL (CMC), and 0.25 mg/mL (TPP). The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one. Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks. Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain. The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively. The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 ㎍/mL, respectively. The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain. Conclusions: This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.

• Korean Journal of Radiology
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• v.24 no.8
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• pp.729-738
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• 2023

Objective: When multiple surveillance mammograms are performed within an annual interval, the current guidance for oneyear follow-up to determine breast cancer status results in shared follow-up periods in which a single breast cancer diagnosis can be attributed to multiple preceding examinations, posing a challenge for standardized performance assessment. We assessed the impact of using follow-up periods that eliminate the artifactual inflation of second breast cancer diagnoses. Materials and Methods: We evaluated surveillance mammograms from 2007-2016 in women with treated breast cancer linked with tumor registry and pathology outcomes. Second breast cancers included ductal carcinoma in situ or invasive breast cancer diagnosed during one-year follow-up. The cancer detection rate, interval cancer rate, sensitivity, and specificity were compared using different follow-up periods: standard one-year follow-up per the American College of Radiology versus follow-up that was shortened at the next surveillance mammogram if less than one year (truncated follow-up). Performance measures were calculated overall and by indication (screening, evaluation for breast problem, and short interval follow-up). Results: Of 117971 surveillance mammograms, 20% (n = 23533) were followed by another surveillance mammogram within one year. Standard follow-up identified 1597 mammograms that were associated with second breast cancers. With truncated follow-up, the breast cancer status of 179 mammograms (11.2%) was revised, resulting in 1418 mammograms associated with unique second breast cancers. The interval cancer rate decreased with truncated versus standard follow-up (3.6 versus 4.9 per 1000 mammograms, respectively), with a difference (95% confidence interval [CI]) of -1.3 (-1.6, -1.1). The overall sensitivity increased to 70.4% from 63.7%, for the truncated versus standard follow-up, with a difference (95% CI) of 6.6% (5.6%, 7.7%). The specificity remained stable at 98.1%. Conclusion: Truncated follow-up, if less than one year to the next surveillance mammogram, enabled second breast cancers to be associated with a single preceding mammogram and resulted in more accurate estimates of diagnostic performance for national benchmarks.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.2A
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• pp.80-85
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• 2003

This paper proposes Galois Field Arithmetic Unit(GFAU) whose structure does addition, multiplication and division in GF(2m). GFAU can execute maximum two additions, or two multiplications, or one addition and one multiplication. The base architecture of this GFAU is a divider based on modified Euclid's algorithm. The divider was modified to enable multiplication and addition, and the modified divider with the control logic became GFAU. The GFAU for GF(2193) was implemented with Verilog HDL with top-down methodology, and it was improved and verified by a cycle-based simulator written in C-language. The verified model was synthesized with Samsung 0.35um, 3.3V CMOS standard cell library, and it operates at 104.7MHz in the worst case of 3.0V, 85$^{\circ}C$, and it has about 25,889 gates.

• JSTS:Journal of Semiconductor Technology and Science
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• v.4 no.2
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• pp.106-109
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• 2004

A high-density 3-transistor cell one-time programmable (OTP) ROM array using standard CMOS Gate-Oxide antifuse (AF) is proposed, fabricated, and characterized with $0.18{\mu}m$ CMOS process. The proposed non-volatile high-density OTP ROM is composed of an array of 3-T OTP cells with the 3-T consisting of an nMOS AF, a high voltage (HV) blocking transistor, and a cell access transistor, all compatible with standard CMOS technology.

• Parasites, Hosts and Diseases
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• v.33 no.4
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• pp.357-364
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• 1995

The protozoan parasite, Entcmoeba histoIWticc, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to 5. histoIHtica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the (jading that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to 5. histolvtica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to 5. histoLvtica trophozoites for 30 minutes, 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polprnerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1 H 107 molecules/ug of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA) . Lysates of 5. histoIVtica also induced expression of mRNA for IL-8 in colon epithelial cells. These results sugf:esc that acute inflammatory reaction by 5. histoIVticc may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.

• Korean Journal of Human Ecology
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• v.20 no.5
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• pp.1075-1091
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• 2011

The purposes of this study were to investigate the actual state of indoor air quality in semi-underground multi-family housing units in early summer, to analyze the influencing factors, and to make suggestions for improvement. A series of field investigations were conducted in four target units between June 22, 2010, and June 28, 2010. The field investigations included measurements of indoor air quality as well as the observation of architectural characteristics and living conditions. In addition to the field investigation, on-site questionnaire surveys were administrated to residents in 90 units. The findings are summarized as follows: (1) The average $CO_2$ levels in each of the four units ranged from 759ppm to 1885ppm. $CO_2$ levels in three units exceeded the evaluation standard (1000ppm), and one unit was lower than the standard. This unit had smaller number of staying person and a large amount of ventilation than others; (2) the average CO levels in each unit were almost 0ppm, but 0.1~1.1 levels of CO were revealed at several times; (3) the average PM-10 levels ranged from $8{\mu}g/m^3$ to $40{\mu}g/m^3$, which distributes within the standard ($150{\mu}g/m^3$). The influencing factors of PM-10 levels were analyzed as inflow from outside, the use of a gas range, and the residents' activities; (4) therefore, it was evaluated to be necessary to improve ventilation for the indoor air quality of semi-underground housing in aspect of $CO_2$ levels.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.55.1-55.1
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• 2017

HectoMAP is a dense redshift survey of red galaxies covering a 53 square degree strip of the northern sky, and Horizon Run 4 is one of the densest and largest cosmological simulations based on the standard Lambda cold dark matter model. We use HectoMAP and Horizon Run 4 to compare the physical properties of observed large-scale structures with simulated ones in the redshift range 0.22

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.4
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• pp.2443-2449
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• 2014

The recent increase in the number of network RTK users has led to a longer wait time for connecting to the existing VRS service, and consequently caused inconvenience such as a longer turnaround time for surveys. To resolve such issue, the NGII has developed the FKP service that involves a one-way communication. However, it is not spreading across the market as fast as anticipated, due to accuracy problems in the field. This study uses eight unified control points for a reliability assessment to compare and analyze the survey results obtained through the VRS and FKP services in various ways. The analysis reveals that the standard deviation of the FKP service is ${\pm}0.02m$, which is two times larger than that of the VRS, whilst its standard errors are X:-0.025m and Y:0.011m, proving that it is reliable to be used in the field. Therefore, we expect that the FKP service is going to be more broadly utilized for field surveys in the future.

• Journal of Biosystems Engineering
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• v.29 no.6 s.107
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• pp.531-538
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• 2004

This study examined the muscle fatigue of the erector spinae according to box size during lifting activity. Ten healthy adult man participated in the experiment for lifting the boxes 10 times with 0.2 lift/s frequency. Bilateral surface EMG (Electomyogtamphy) recordings were made regarding M. (Muscle) Longissimus, M. Iliocostal and M, Multifidus activity throughout lifting working. The median frequency in back muscle fatigue was investigated using power spectral analysis of surface EMG. When the box larger than shoulder width was lifted, the muscle fatigue of M, Multifidus was shown larger than one of the other muscles. Accordingly, the result far M. Multifidus can be applied in optimizing design parameter of box. Also, the standard of box width for box makers have to be below about 420 mm, being average shoulder width of adult male.

• Journal of the Korean Chemical Society
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• v.8 no.3
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• pp.113-120
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• 1964

The critical examination of the spectrophotometric method for determining microgram quantities of phosphate by the n-butyl acetate extraction as molybdophosphoric acid and subsequent development of the molybdenum blue has been made. In this procedure from 2 to 8 ${\mu}g$. of phosphate-phosphorus can be determined under optimum conditions. The final concentration of ammonium molybdate and the final acidity of perchloric acid for the formation of heteropoly acid are suitable to be ranges of 0.5 to 1.1% and 0. 5 to 1. 1 N respectively, and subsequently extracted with 10 ml. of n-butyl acetate. The extract is developed to molybdenum blue with 5.0 ml. of 1. 3% stannous chloride in 1N hydrochloric acid. The color is stable for at least one hour in the use of perchloric acid for the condensation. In order to determination of submicrogram amounts of phosphate, the sensitivity of the molybdenum blue method is hardly sufficient, a sensitive and stable molybdenum(V)-thiocyanate complex method has been investigated. By the procedure less than 1.2 ${\mu}g$. of phosphate-phosphorus can be determined with an accuracy of less than 5% the relative error. The molybdenum(Ⅵ) extracted by the above procedure is reduced to molybdenum(V) in the extract directly with a solution of 4 to 10% of stannous chloride, 0.5 to 1.5 mM of copper, and 0.1 to 0.9 N of perchloric acid as final concentration in 4.3 to 6.3 N of hydrochloric acid or 9.0 to 13.0 N of sulfuric acid by heating for one minute in boiling water, after cooling, the molybdenum(V)-thiocyanate complex color is developed by adding 6.0 M ammonium thiocyanate solution making the final concentration to be in a range of 0.4 to 0.9 M. This procedure the very sensitive, reliable, and stable can be applied to determining submicrogram amounts of phosphate in natural waters with a precision of 1.6 ${\times}\;10^{-2}$ the standard deviation as absorbance.