• Journal of the Korean Institute of Rural Architecture
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• v.5 no.1
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• pp.32-41
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• 2003

After the 1988 Seoul Olympics, a lot of Culture & Arts Centers have been built in Korea. There have been raised questions on the frequency of room use and the occupancy of seats, but there are not enough architectural data for the Culture & Arts Centers in Korea. This paper focuses on the investigation of the architectural features of the Culture & Art Centers, such as the spatial sizes, the composition ratio of the performing art hall area to the overall facility area, the sizes and proportions of seat zone to stage zone, the average area per one person at the seat zone, the wide and the height of the procenium, the height of fly loft, and the number of users per one program. We could find out some meaning results. The composition ratios of the performing art hall area to the overall area of the facilities distribute with a wide range. The floor areas of 1500 through 2000 seat performing art halls are larger than $13,000m^2$. The average area per one person for seat zone and stage zone is approximately $0.8m^2$. All facilities we investigated have the types of the procenium stage. Almost all of the facilities have high quality stage conditions and equipments, but few programs use the various stage equipments and all of the main, side, and rear stages. To resolve this problems, various programs have to be developed for encouraging more people to use the Culture & Art Centers.

• Korean Journal of Animal Reproduction
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• v.19 no.2
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• pp.89-93
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• 1995

In this study was demonstrate that retrovirus-mediated gene transfer is one of the promising alternatives to the conventional pronuclear DNA microinjection approach, especially in transferring the exogenous genes into the boving embryos. By co-culturing of zona of zona-free one-cell stage embryos with the retrovirus-producing cells for 24 hours followed by 6 days of culture in virus-free medium, we could get morulae and blastocysts expressing the E. coli LacZ genes which were transferred by our retrovirus vector. The results obtained in this study are summarized as follows : 1. Addition of 5$\mu\textrm{g}$/ml of polybrene in the embryo and virus-producing cell co-culture medium did not affect development of zona-free one-cell embryo. 2. Compared with the intact embryos removal of zona at one-cell stage before co-culturing with the virus-producing cells for one day caused only slight decrease of embryo develpment. 3. Co-culture of 625 zona-free one-cell stage embryos with the virus-producing cells resulted in 65(10.4%) morulae or blastocysts, and 12.3%(8/65) of the morulae or blastocysts were E. coli LacZ positive.

• Korean Journal of Pharmacognosy
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• v.17 no.1
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• pp.7-11
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• 1986

The young leaf of Perilla species was cultured by two stage culture system using the medium containing mevalonic acid lactone. The growth rate and productivity of essential oil of callus were increased. The essential oil from intact plant and callus was also analysed. Sesquiterpene hydrocarbons and one sesquiterpene alcohol were identified in essential oils of callus.

• International Journal of Advanced Culture Technology
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• v.7 no.4
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• pp.96-103
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• 2019

In this paper, we propose two-stage multichannel architecture for oyster product management system, called cloud stage and agent stage. There are two communication channels at each stage. In cloud stage, the embedded system in the smart scale communicates with the server through two channels, Ethernet or 3G/LTE mobile communication. In agent stage, PCs and smart phones called agents communicate with the server also through Internet and 3G/LTE mobile communication. Compared with previous system in which the amount of the oyster produced in oyster workplaces could be monitored only at the console of only one oyster main server, developed system makes it possible to monitor the amount of produced oyster at several PCs (or smart phones). In addition to the amount of oysters produced at all oyster workplaces the environment of oyster workplaces such as temperature and humidity can be monitored on agents to judge the freshness. Two-stage architecture with multiple channels makes it possible to monitor the amount of oyster product and environment of the oyster workplace at any place in real time.

• Korean Journal of Animal Reproduction
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• v.18 no.1
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• pp.7-13
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• 1994

To verify in vivo viability of IVF-derived bovine embryos, morula and blastocysts that developed from in vitro matured and fertilized ova were transferred to the uteri of recipient cows and normal calves were produced. To produce IVF-derived bovine morula or blastocysts, ova matured and fertilized in vitro were cultured in culture medium for 7~8 days at 39$^{\circ}C$ under the humicified atmosphere of 5% CO2. Two different culture systems, a co-culture system with TCM-199 and bovine epithelial cells (BOEC) and CR1aa without somatic cell support, were compared. Cleavage rates to 2~8 cell stage and developmental rates of IVF-derived bovine embryos to blastocyst stage were not different between co-culture system (51.3 and 14.0%) and CR1aa medium (60.4 and 22.1%), respectively. Embryos were classified into three grades by embryo quality and then one or two embryos in higher quality(A and B grades) were transferred to the uterus of recipients. In this study Korean Native calf was first born after transfer of IVF-derived embryos. Total four live calves were normally developed to term from IVF-derived bovine blastocysts and one female fetus was still-born approximatedly 8 months of gestation, but there was no pregnancy after transfer of morula. Therefore, normal calves could be produced after transfer of IVF-derived bovine embryos cultured in CR1aa medium without somatic cell support. In addition, our results suggest that in transfer of IVF-derived bovine embryos blastocyst stage is better than morula.

• Journal of Family Resource Management and Policy Review
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• v.16 no.4
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• pp.1-22
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• 2012

The purpose of this study is to determine the characteristics of Filipino marriage immigrants' acculturation stages in regards to their Korean family life culture and to identify the source of the support for each stage, hence to provide information for educational programs that would promote successful acculturation for each stage. The following findings have been obtained through in-depth interviews with 18 female Filipino immigrants to Korea. In a range of obstacles from the Honeymoon Stage to the Confusion stage, the first are the language and the aspects of the food/cooking/ingredients/diet that are different from their own culture. Especially, pregnancy/childbirth is a major change in one's life and the biggest challenge in the acculturation process. As food and cooking are the first change that the immigrants have to face and get accustomed to in the early stage of their Korean life, the food culture is rather easier for the immigrants to get accustomed to than other parts of Korean life. From the Honeymoon Stage to the Harmony Stage, the immigrants make efforts to help their family in the home, while they look to their future in their children during the Autonomy Stage. Regardless of how long they have been in Korea, from the Honeymoon Stage to the Autonomy Stage, the immigrants have a hard time with the patriarchal environment in Korea due to the bilateral nature of kinship in the Philippines. Secondly, the immigrants receive the most support from their husband, family, and the tutors in Korean culture, while their mothers-in-law are the main source of the support for the Korean diet. At the Confusion Stage, the immigrants start visiting the regional multicultural family support centers and get help from the friends they meet there while depending on the TV for cooking tips. From the Harmony Stage, they may seek a job through the community network with their own effort and their children's help. In the Autonomy Stage, they are concerned about their children rather than their own parents, and they find their own identity as a Korean and realize that their effort is important.

• Journal of Korean Society of Rural Planning
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• v.15 no.4
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• pp.173-182
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• 2009

This study is a plan for the Jeju Sanghyo Botanical Garden construction, and it is prepared to maintain the identity of the local landscape by composing the garden using the local landscape resources. The goals of becoming one of world's best, garden centered botanical garden and at the same time one that represents culture of Jeju. Unusual species are included in the collection and with the general collection, special collection and exhibition by different kinds are planned to be offered to the public. The garden will demonstrate the world's various types of gardens in a compressed way. It is our endeavor to become center of culture beyond plant education and exhibition, drawing a large number of people to our garden. Moreover, conservation of Jeju's unique botanic resources will be carried out and the garden will show the garden culture. Due to the size of the site, different themes will be exhibited in one zone in a parallel. Unique combination of each local landscape resource will create own theme. The display will be divided into three different stages: the 1st stage is an educational exhibition, assisting more understanding of distinguishing different locations, the second is exhibition by various species and the final stage will show an ideal way to develop a garden with harmony of various plant species.

• International Journal of Advanced Culture Technology
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• v.8 no.2
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• pp.184-191
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• 2020

This study is conducted to find out the nurses' character for care and the workplace bullying for the early stage nurses working in the clinical field and their relation in order to understand the factors which would affect the consequence of the workplace bullying. Data collected from 131 early stage nurses working at one general hospital for 7 months was used for this study. The data was analyzed with descriptive statistics, Independent t-test, one-way ANOVA, Pearson's correlation coefficient, Hierarchical multiple regression by using SPSS/WIN 21.0. It was found that high the nurses' character for care was related to the lower workplace bullying (type and consequence) (r=-.31, p<.001; r=-.26, p=.003) and that the worse the score in the type of bullying wasthe higher consequence (r=.54, p<.001). The multiple regression analysis showed that the factors affecting the consequence of the workplace bullying were the type of bullying (β=.48), dissatisfaction with nursing (β=.22), and moderate satisfaction with nursing (β=.19) and that the explanation of the model by variables was 32.5%. Based on the results of the study, it is suggested that the effort of lowering the occurrence of the type of bullying and the strategy of enhancing the satisfaction with nursing such as improvement in the quality of nursing education be made in order to improve the consequence of workplace bullying at the workplace for the early stage nurses.

• Reproductive and Developmental Biology
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• v.31 no.2
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• pp.91-96
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• 2007

In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. 1his study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 ($250{\sim}270$ mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose ($300{\sim}320$ mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.343-348
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• 1989

Production of shikonin derivatives by Lithospermum erythrorhizon cells by using polyurethane foam was invesliigated. Shikonin derivatives were effectively adsorbed mostly by phase distribution to polyurethane matrices and their production increased significantly compared to the suspension culture. The enhanced production of shikonin was probably due to more facilitated cell to cell con-tact and lowered intracellular shikonin concentration, both of which are known to be favorable for plant secondary metabolite production. In order to improve the process productivity, tell culture was conducted under various culture conditions: Of them, Schenk and Hildebrandt medium containing indole-3-acetic acid (1.75mg/ι) and kinetin (0.1mg/ι) was considered most appropriate for shikonin production. Production of shikonin increased about 4.5 times in the Schenk and Hildebrandt medium containing indole-3-acetic acid (1.15mg/ι) and kinetin (0.1mg/ι) when compared to the same medium containing p-chlorophenoxyacetic acid (2.0mg/ι) and kinetin (0.1mg/ι). When poly-urethane was used as the support material, a single-stage system was more preferred to the conventional two-stage culture system in terms of shikonin productivity.