• Journal of Digital Contents Society
• /
• v.6 no.1
• /
• pp.13-17
• /
• 2005

With the development of internet and networking technology, on-line remote educations in many area are being experimented. As on-line remote educations become more common, many people make multimedia contents for educational purpose. We designed and implemented a lecture authoring tool which provide easy way to build educational multimedia contents. We implemented the authoring tool with Java JMF, which can be executed any computer hardware. The purpose of this paper is to share the experience of implementing authoring tool and the implementation result of the authoring tool using Java JMF.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2009.01a
• /
• pp.732-736
• /
• 2009

In this paper we focus on EPI (Epipolar-Plane Image), the horizontal cross section of Ray-Space, and we propose a novel method that chooses objects we want and edits scenes by using multi-view images. On the EPI acquired by camera arrays uniformly distributed along a line, all the objects are represented as straight lines, and the slope of straight lines are decided by the distance between objects and camera plane. Detecting a straight line of a specific slope and removing it mean that an object in a specific depth has been detected and removed. So we propose a scheme to make a layer of a specific slope compete with other layers instead of extracting layers sequentially from front to back. This enables an effective removal of obstacles, object manipulation and a clearer 3D scene with what we want to see will be made.

• Journal of Broadcast Engineering
• /
• v.25 no.1
• /
• pp.48-57
• /
• 2020

Recently, with the rapid development of image recognition technology, the demand for object analysis in road CCTV videos is increasing. In this paper, we propose a method that can adaptively find the counting line for traffic analysis in road CCTV videos. First, vehicles on the road are detected, and the corresponding positions of the detected vehicles are modeled as the two-dimensional pointwise Gaussian map. The paths of vehicles are estimated by accumulating pointwise Gaussian maps on successive video frames. Then, we apply clustering and linear regression to the accumulated Gaussian map to find the principal direction of the road, which is highly relevant to the counting line. Experimental results show that the proposed method for detecting the counting line is effective in various situations.

• The Journal of the Korea Contents Association
• /
• v.11 no.6
• /
• pp.197-204
• /
• 2011

In line with the development of Media, the use of character has been developed in various ways. Specially, it has been emerged the functions of written language of visualization and delivery of emotion in the digitalized environment due to speedy growth of internet and Mobile. The emoticons emerged as a tool for the convenience of communication proved to have taken effective role in the part of communication culture. Particularly, Hangul emoticons which contains the meaning of communication and sharing in tandem with the principle of Hangul invention, provides convenience in combination & use due to its structural and design characteristic for which it plays a significant role of visual element. Hangul emoticon is definitely a part of Hangul culture and in view of globalization of visual language in connection with media development, there is a huge potential being to be used as a international communication tool. In this study, it will be reviewed on the increased utilization of Hangul emoticons and related public issues as being a part of typography media, in view of the generalization of private media as well as change of subjective user.

• Asian-Australasian Journal of Animal Sciences
• /
• v.10 no.2
• /
• pp.233-239
• /
• 1997

A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.10 no.3
• /
• pp.198-204
• /
• 2005

Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

• KSBB Journal
• /
• v.27 no.3
• /
• pp.186-194
• /
• 2012

Various human host cell lines, which are more effective than the other original human cell lines, have been developed and used. Highly efficient human cell line can be obtained from the fusion between human embryonic kidney 293 (HEK293) and human Burkitt's lymphoma cells (Namalwa). Fused cell line has the advantages of both cell lines such as the high transfection efficacy of HEK293 cells and the constitutive expression of Epstein-Barr virus (EBV) genome which is related with high expression of target protein and anti-apoptotic growth of Namalwa cells. In this study, characterization of two original cell lines was performed by using design of experiment (DOE) considering cell maintenance, media development, optimization of culture condition, and scale-up. The formation of aggregates was apparent with high glutamine concentration at more than 6 mM. Supplementation of hydrolysates showed positive effects on the growth performances of HEK293 cells. On the contrary, Namalwa cells showed negative results. It was confirmed that Namalwa cells were more sensitive to lower temperature at $35^{\circ}C$ and hyperosmotic condition over 260 mOsm/kg. In addition, both cell lines showed limited growth in 3-L bioreactor due to shear stress.

• Korean Institute of Interior Design Journal
• /
• v.22 no.5
• /
• pp.293-301
• /
• 2013

The purpose of this study was to find the space perception by the scene and to analyze the characteristics in the exhibition space. The data for the analysis was collected through a questionnaire survey method. The questionnaire is composed of a scene selection on the panoramic photographs of the exhibition space. 'The 4 Rivers Cultural Center - The ARK' was selected as the subject of this survey. The results of the study are presented as follows : First, the space perception perceived imaged scenes rather than the specific elements of space. Second, the space perception was revealed through the 'Form', 'Materials', 'Light', 'Lighting', 'Furniture' and 'Media' of notable elements. Also that was perceived by forming 'Line', 'Surface' and 'Volume'. Particularly, the perception of 'Surface' was higher than 'Line' and 'Volume'. 'Surface' was perceived mostly through the 'Color'. The perception of 'Line' was positive to 'Boundary' or 'Continuous placement of the light'. In addition, the perception of 'Volume' was positive to 'The curve of the form', 'Transparent material', 'Three-dimensional elements'. Finally, The type of space perception was chosen as 'Extension', 'Center', 'Deep', 'Boundary' and 'Formative'. In this way, the results of this study will set a foundation for developing design methods to induce the space perception by the scene in the exhibition space.

• Journal of Biomedical Engineering Research
• /
• v.32 no.3
• /
• pp.185-190
• /
• 2011

The purpose of this study is to investigate the influence of organism vigor information solution(OVIS) on the animal cell growth and to set up a condition for cell culture. We investigated the reactions on the MG-63 and MCF-7 cell line in mixture culture media with various amount of REVIEW solution, which is an example among various OVIS. DMEM-HG was used as basic media. The concentration range of the mixture was limited from 0% to 15%. MTT assay is used for cell viability test. The cell was incubated for 14days and the MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. We used the ELISA reader to measure the Optical Density(O.D) at 595 nm wavelength filter. MCF-7 was linearly proliferated according to culture time and concentrations of OVIS. On the other hand, MG-63 cells were measured the highest O.D value at 12%. The growth rates of both cells cultured in mixed culture media with OVIS are much higher than those in only basic media, DMEMHG, after 14days. It was confirmed that cell cultured at OVIS grows rapidly at certain period although cells showed a negative effect in initial stage.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2009.11a
• /
• pp.285-285
• /
• 2009

Due to a development of a modem technology as Power Line Communication(PLC) over 200 Mbps, the high-speed multi-media data trasmission could be currently possible. In This paper we develop a high quality media transmitter-receiver based on merging the HomePlug AV, which is 200 Mbps class PLC technology and HDMI Interface technology. Smart Live 6 software were used for the assessment of audio property. As the result of measurement of the HD class images by capturing from the receiver of the PLC, the quality of images couldn't be confirm any deterioration, which has compared with original reflections. In case of audio part as the result of confirmation of the Phase, Magnitude, it has been confirmed that over 90% of nomal transmition and receiving of acoustic signal.