• Title/Summary/Keyword: oligosaccharide

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Modeling the effect of mass transfer on the kinetics of fructo-oligosaccharide production by immobilized cells (고정화 세포에 의한 Fructo-oligosaccharides 생산 반응메카니즘에서 물질전달 영향에 대한 수학적 모델)

  • 윤종원;전영중이민규송승구
    • KSBB Journal
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    • v.8 no.3
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    • pp.266-271
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    • 1993
  • The effective diffusion coefficients of glucose, sucrose and fructo-oligosaccharides in Ca-alginate gel beads at high concentration of sucrose solutions were investigated at $50^{\circ}C$. A mathematical model for the kinetics of fructo-oligosaccharide production using immobilized cells was proposed and compared with experimental results varying the bead size, the substrate concentration and the bead ratio. Very low values of diffusion coefficients ranging $1.2-7.6\times10^{-7}\textrm{cm}^2$/sec were obtained, and the predicted results were in good agreement with experimental ones in all cases tested.

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Effects of Oligosaccharide-Supplemented Soy Ice Cream on Oxidative Stress and Fecal Microflora in Streptozotocin-Induced Diabetic Rats (당뇨쥐에서 올리고당 첨가 콩아이스크림이 산화스트레스와 장생태에 미치는 효과)

  • Her, Bo-Young;Sung, Hye-Young;Choi, Young-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.10
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    • pp.1536-1544
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    • 2005
  • We have investigated physiological effects of soy ice cream with oligosaccharide on oxidative stress and fecal microflora in streptozotocin-induced diabetic rats. Parched soybean powder (7.6$\%$, w/w) substituted skimmed milk and cream, soybean oil (7.6$\%$, w/w) for milk oil, and fructooligosaccharide (9.5$\%$, w/w) for sucrose. Five types of ice cream were prepared: regular, oligosaccharide-supplemented regular, soy, oligosaccharide - supplemented soy, and oligosaccharide - supplemented black soybean ice cream . Freeze - dried ice cream was supplemented to AIN93-based diets at 30$\%$ (w/w) containing 6.5$\%$ soy and 4.5$\%$ fructooligosaccharide. Diabetes was induced by intramuscular administration of streptozotocin, and experimental diets were given for 4 weeks. Plasma concentration of thiobarbituric acid reactive substances (TBARS) was significantly increased in the diabetic rats compared with the normal rats, then was significantly decreased with feeding soy ice cream containing diet compared with regular ice cream containing diet among the diabetic groups. The levels of TBARS in liver were decreased in the rats that were fed either soy or oligosaccharide ice cream compared with the rats that were fed regular ice cream. Erythrocyte superoxide dismutase activity was significantly increased in the rats fed soy ice cream compared with the rats fed regular ice cream. Erythrocyte glutathione peroxidase and catalase activities were significantly increased in the rats fed black soybean ice cream. Fecal concentrations of Lactobacilli were significantly higher in the rats fed soy ice cream and oligosaccharide- supplemented soy ice cream than that of the rats fed regular ice cream. Fecal concentrations of Bifidobacteria were significantly higher in the rats fed oligosaccharide- supplemented soy ice cream than that of the rats fed regular ice cream. In conclusion, oligosaccharide- supplemented soy ice cream suppressed lipid peroxidation and improved the got microbiota in diabetic rats compared with milk-based regular ice cream.

Effects of Alginate Oligosaccharide on Lipid Metabolism in Mice Fed a High Cholesterol Diet (알긴산 올리고당이 고콜레스테롤식이를 급여한 마우스의 지질대사에 미치는 영향)

  • Back, Su-Yeon;Kim, Hyun-Ku;Jung, Seung-Ki;Do, Jeong-Ryong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.491-497
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    • 2014
  • This study investigated the effects of alginate oligosaccharide on lipid metabolism in mice fed a high-cholesterol diet for 6 weeks. Male apoE mice were assigned to four groups: normal diet group (N), high-cholesterol diet group (HC), HC with 5% alginate oligosaccharide group (HC-AOL), and 10% alginate oligosaccharide group (HC-AOH). Epididymal adipose tissue and kidney adipose tissue weights were significantly reduced in the HC-AOH group by 131.4% and 148.4%, respectively, as compared to the HC group. Serum total cholesterol (TC), triglyceride (TG), and LDL-cholesterol levels were also significantly reduced in the HC-AOH group by 57.5%, 51.4%, and 82.9%, respectively, as compared to the HC group. Hepatic TC and TG levels in the HC-AOH group were significantly reduced by 72.3% and 33.5%, respectively, as compared to the HC group. These results indicate that alginate oligosaccharide might improve lipid metabolism and reduce fat accumulation.

Quality Characteristics and Antioxidant Activity of Dioscorea bulbifera, under Various Oligosaccharide Pre-Soaking Condition (당 침지액 농도에 따른 건조 둥근 마의 품질 특성 및 항산화 효과)

  • Jo, Hye Min;Kim, Hee Young;Heo, Yoo Jeong;Song, Da Hye;Kim, Gyeong-Ji;Kim, In-Su;Yoon, Jin-A;Chung, Kang-Hyun;An, Jeong Hee
    • The Korean Journal of Food And Nutrition
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    • v.30 no.6
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    • pp.1176-1183
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    • 2017
  • This study was investigated the quality characteristics and antioxidant of dried Dioscorea bulbifera with various pre-soaking concentrations of oligosaccharide. Dioscorea bulbifera are prepared by additions of 0, 4, 6, 8 and 10% oligosaccharide solution, and dried at $50^{\circ}C$. The effects of pre-soaking percent of Dioscorea bulbifera slices were evaluated by the moisture, soluble solid, pH, titratable acidity, color, browning degree, texture, antioxidant activities and sensory test. According to the percent of pre-soaking oligosaccharide solution was increased, the moisture was increased but soluble solids and titratable acidity were decreased. With respect to the result of colors, Dioscorea bulbifera slices that underwent the 10% pre-soaked process (85.86%) were lighter than control (73.88%). However, the redness and yellowness scores were the lowest than control. The springiness and cohesiveness of texture showed no significant differences among all groups. Gumminess and chewiness of texture results were increased according to per-soaking concentration increase. Also the polyphenol, flavonoid and DPPH (${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activities were significantly increased with increasing immersion concentration. The Dioscorea bulbifera slices supplemented with 6% pre-soaking oligosaccharide solution treatment showed the highest total sensory score. The results of our study indicated that when pre-soaking oligosaccharide solution is used to immerse the Dioscorea bulbifera slice, it has browning inhibition and antioxidant effect.

Studies on the Browning Reaction of Sugar Derivative Sweeteners (당유도체 감미료의 갈색화반응에 관한 연구)

  • Lee, Cherl-Ho;Han, Bok-Jin;Kim, Na-Young;Lim, Jae-Kak;Kim, Bong-Chan
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.52-56
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    • 1991
  • The browning reaction of sugar derivatives, fructo-oligosaccharide, high maltose syrup(HMS), sorbitol and maltitol, and their effect on the appearance of jam and candy were investigated. The spectrophotometrie scanning of the absorbance between 230 nm and 700 nm could demonstrate the heat induced browning of the sugar derivatives. Fructo-oligosaccharide and HMS showed sharp increase in absorbance at 270-330 nm range by heating at $100{\sim}120^{\circ}C$ for 1 hr but sorbitol and maltitol did not show the increase in absorbance. When the pH was lowered red from neutral to 2.0, the absorbance of HMS and sucrose increased sharply, showing that these substances are relatively unstable in acidic heating compared to fructo-oligosaccharide. The addition of glycine enhanced the browing reaction of fructo-oligosaccharide and HMS, whereas little change was observed with sucrose, sorbitol and maltitol. These browning characterisitcs of sugar derivatives were reflected to the color development of apple jam and candy where they were used. Both fructo-oligosaccharide and HMS increased the yellowness of these products, while sugar alcohols reduced the yellowness compared to sugar.

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Retrogradation of Rice Starch Gels by Additives (첨가물이 쌀전분겔의 노화에 미치는 영향)

  • Song, Ji-Young;Kim, Jeong-Ok;Kim, Sung-Kon;Kim, Kwang-Joong;Shin, Mal-Shick
    • Applied Biological Chemistry
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    • v.40 no.4
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    • pp.289-293
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    • 1997
  • The effects of different types of additives(sucrose fatty acid ester(SE), oligosaccharide and alum) on 40% nonwaxy rice starch gels stored at $20{\pm}1^{\circ}C$ were investigated by ${\alpha}-amylase-iodine$ method, X-ray diffractometry and differential scanning calorimetry. The addition of SE retarded the retrogradation of gels throughout storage period and the antistaling effect of SE was increased with increasing concentration. Oligosaccharide(0.1%) inhibited the retrogradation, but oligosaccharide(0.1% or 0.5%) retarded the retrogradation at early stage of storage, and stimulated the retrogradation after 3 days. Alum of 0.1% level retarded the retrogradation, but 0.3% level activated the retrogradation after 24 hours and 0.5% level did after 12 hours. The effect of additives on the retrogradation of rice starch gels showed the different tendencies at the level of additives. On 0.1% level, the effect of those was similar but on 0.5%, the degree of retardation increased in order of oligosaccharide, SE1170, alum, no addition for up to 2 days of storage and SE1170, no addition, oligosaccharide, alum for 3 days of storage.

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Changes of Oligosaccharide and Free Amino Acid in Soy Yogurt Fermented with Different Mixed Culture (혼합균주를 이용한 대두유의 발효에 따른 당 및 유리아미노산의 변화)

  • Kim, Cherl-Hyun;Shin, Yong-Kook;Baick, Seung-Chun;Kim, Soo-Kwang
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.739-745
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    • 1999
  • This study was carried out to investigate the oligosaccharide and amino acid utilization by mixed cultures during soy yogurt fermentation. Three types soy yogurt were prepared by fermenting with Lactobacillus acidophilus and Streptococcus thermophilus, Streptococcus thermophilus and Saccharomyces uvarum, Lactobacillus acidophilus and Saccharomyces uvarum. The utilized amount of oligosaccharide and amino acid was determined by HPLC during the fermentation period. The oligosaccharide and amino acid utilization efficiency of S. thermophilus and Sac. uvarum was greater than the other mixed cultures. It was found that Sac. uvarum produced enzymes which can convert oligosaccharide and common sugars in soy milk into glucose, galactose and fructose which can be fermented by L. acidophilus and S. thermophilus, and in turn stimulated acid production and amino acid utilization of the latter.

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Effect of Inclusion of Chitosan-Oligosaccahariede in Drinking Water on the Blood Component Profile, Immunity and Antioxidative Enzyme in Broiler Chickens (키토산 올리고당의 음수내 급여가 육계의 혈액성상, 면역력 및 항산화 효소계에 미치는 영향)

  • Park, Seong-Bok;Kim, Sang-Woo;Kim, Young-Sin;Na, Chong-Sam;Shim, Kwan-Seob
    • Korean Journal of Organic Agriculture
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    • v.22 no.3
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    • pp.483-490
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    • 2014
  • The study was conducted to the effect of inclusion of chitosan-oligosaccharide in drinking water on the blood component profile, immunity and antioxidative enzyme of broiler chickens. A total of 28,000 broiler chickens (Arbor Acre) at 1 days of age were fed the commercial diet until 35 days of age, the treatment divided into two treatments, normal control group and chitosan-oligosaccharide in drinking water group. In concentration of glucose, treatment was significantly decreased than control (P<0.05), but not statistically different on the Triglyceride, Total cholesterol, HDL-cholesterol and LDL-cholesterol in the Blood component profile of broiler chicks. The concentration of glutamic oxalacetic transaminase (GOT) was statistically different but the concentration of glutamic pyruvate transaminase (GPT) was significantly decreased in treatment fed chitosan-oligosaccharide than control (P<0.05). Immunoglobullins in the blood, concentration of IgG was not significantly different among control and treatment but concentration of IgM was significantly increased in fed chitosan-oligosaccharide than control (P<0.05). Antioxidant and super oxide dismutase (SOD) was not different among control and treatment and concentration of catalase and glutathione peroxidase were significantly increased in fed chitosan-oligosaccharide than control (P<0.05).

Manufacture of Antimicrobial Paper for Food Products (식품포장용 항균지 제조)

  • 이명구;이상명;오덕환
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.32 no.1
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    • pp.65-71
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    • 2000
  • Chitosan oligosaccharide, chitosan polysaccharide and monolaurin were used to impart the antimicrobial properties onto paper for food products . Staphylococcus aureus (ATCC 25923) was used as test microorganism and shake flask test was carried out in order to examine the antimicrobial activity of each sample. Chitosan oligosaccaride showed the greatest antimicrobial effect among them and gave the promising result even at the concentration of 0.005%. Its antimicrobial power was enhanced when it is used with nomolaurin together. Chitosan oligosaccharide improved the tensile strength of antimicrobial paper.

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Sialylated oligosaccharide analyses using high-performance liquid chromatography with a fluorescence detector

  • Cho, Due-Hyeon;Ryu, Chang-Soo;Park, Jee-Hun;Kim, Ha-Hyung
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.316.1-316.1
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    • 2003
  • N-acetylneuraminic acid is one of the major derivatives of sialic acid. is widely distributed in mammalian cells as the ${\alpha}$2-3- or ${\alpha}$2-6-linked nonreducing terminal residue of oligosaccharide chains of glycoconjugates, and plays important structural and functional roles at the cell membrane surface. The analysis of sialylated glycoproteins is an important part of glycoprotein characterization, especially because sialylation or desialylation in oligosaccharides often causes dramatic changes in the function of glycoproteins. (omitted)

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