• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.98-105
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• 2018

The sale of brown rice batches composed of rice produced in different years is prohibited in Korea. Thus, new methods for the identification of the year of production are critical for maintaining the distribution of high quality brown rice. Here, we describe the exploitation of an enzyme that can be used to discriminate between freshly harvested and one-year-old brown rice. The degree of enzyme activity was visualized through freshness test with Guaiacol, Oxydol, and p-phenylenediamine reagents. With electronic eye equipment, we selected 29 color codes for identifying new brown rice and old brown rice. The discrimination power of selected color codes showed a minimum of 0.263 to a maximum of 0.922 and an average value of 0.62. The accuracy with which new brown rice and old brown rice could be identified was 100% in principal component analysis (PCA) and discriminant function analysis (DFA). The DFA analysis had greater discriminatory power than did the PCA analysis. A verification test using new brown rice, old brown rice, or a mixture of the two was then performed to validate our method. The accuracy of identification of new and old brown rice was 100% in both cases, whereas mixed brown rice samples were correctly classified at a rate of 96.9%. Additionally, in order to test whether the discriminant constructed in winter can be applied to samples collected in summer, new and old brown rice stored for 8 months were collected and tested. Both new and old brown rice collected in summer were classified as old brown rice and showed 50% identification accuracy. We were able to attribute these observations to changes in enzyme content over time, and therefore we conclude, it will be necessary to develop discriminants that are specific to distinct storage periods in the near future.

• Korean Journal of Poultry Science
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• v.43 no.2
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• pp.97-103
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• 2016

This study aimed of effects of replacing maize with rice or brown rice on laying performance, egg quality, and apparent fecal digestibility of nutrient in Hy-Line Brown laying hens. A total of 200, 25-week old Hy-Line Brown laying hens were randomly assigned to one of 5 treatments, each of which was replicated 4 times. Four experimental diets that were formulated two different grains (brown rice or rice) with two inclusion levels of (15 and 30%) as a replacement of maize. The experimental period was 8 weeks. During the experiment, hens were provided with feed and water ad libitum and were exposed to a 16:8=L:D lighting schedule. There were no differences in feed intake, egg weight, and egg mass during the 8 weeks of the feeding trial among groups. However, hen-day egg production was greater (P<0.05) for Brown rice treatment groups than that for basal and rice treatment groups. In addition, the supplementation of brown rice or rice did not have an effect on eggshell strength, eggshell thickness, and HU during of the feeding trial (Table 4). However, egg yolk color was less (P<0.05) for basal treatment groups than that for among treatment group. ATTD of dry matter (DM) and crude fat were greater (P<0.05) for brown rice 15 treatment than for rice 30 treatment. Crude protein (CP) was greater (P<0.05) for brown rice 15 treatment than for basal treatment. In conclusion, brown rice or rice grains is a good alternative energy feedstuff and can be used in laying hens, totally replacing maize, without any negative effect on the laying performance, egg quality, and nutrient digestibility.

• Korean Journal of Environmental Agriculture
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• v.4 no.1
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• pp.31-36
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• 1985

In order to choose a suitable soil extractant for the prediction model of heavy metal content in brown rice, four extractants-0.1 M HCl, 0.1 M $HNO_3$, 0.1 M $NH_4$-oxalate and 0.001 M 2Na-EDTA, were compared by analyzing 84 soil and 45 brown rice samples collected from paddy fields adj-acent to five old zinc-mining sites. Content of cadmium and zinc in brown rice had the highest correlation coefficient to 0.001 M 2Na-EDTA and 0.1 M HCl extractants, respectively. However, the lead content in brown rice was significantly correlated with only 0.1 M $NH_4$oxalate solution. For the simultaneous prediction of zinc, cadmium and lead in brown rice, 0.1 M $NH_4$-oxalate solution was the most effective. On the multiple analysis by using various chemical characteristics of soils, pH and calcium content of soils were effective variables for the estimation of cadmium content in brown rice, while CEC and magnesium content were more effective for the estimation of zinc content in brown rice. Furthermore, for the estimation of lead content in brown rice, factors such as pH, CEC, calcium, magnesium, potassium and organic matter content were important variables in the multiple regression equation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.5
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• pp.810-814
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• 2004

This research was conducted to evaluate the safety of the herbicide-resistant rice, a genetically modified organism (GMO) developed by the Rural Development Administration by exposing it to 12 months old Sprague-Dawley rats for 8 weeks. The composition of herbicide-resistant brown rice with/without heating treatment was compared with those of conventional Ilpum brown rice with/without heating treatment to assess composition equivalence. Compositional analysis was performed to measure proximates, fiber, and minerals. The nutritional components of herbicide-resistant rice were similar to those of the nontransgenic control or were within the normal range of nontransgenic rice. Four groups of experimental male rats were fed one of the following diets for eight weeks: Ilpum brown rice (I) and its heated rice (IH) as non-GMO, and herbicide-resistant brown rice (G) and its heated rice (GH) as GMO- We checked clinical symptoms (anorexia, salivation, diarrhea, polyuria, anuria, fecal change), food intake, and water consumption every day, change of body weight once a week, and serum biochemistry and organ weights after 8 weeks of experimental feeding We did not find any significant differences in the above-mentioned items. These results suggested that genetically modified herbicide-resistant rice was compositionally equivalent to conventional Ilpum rice, and nutritional characteristics and safety of herbicide-resistant rice in old male rats treated for 8 weeks were not different from those of Ilpum rice, non-GMO.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.85-92
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• 2016

Objectives： This study is experimental comparison of brown rice (BR) and germinated brown rice (GBR) on upper gastrointestinal diseases animal models.Methods： The ICR mice were divided randomly into four groups of six animals each (Normal mice, gastritis mice, gastritis mice treated with BR, gastritis mice treated with 48h GBR). Gastritis was induced by administration of 0.5 mL 150 mM HCl-60% ethanol. Six-week-old male Sprague-Dawley (SD) rats were randomly divided into 7 groups after 1 week adaptation. (Normal rat, reflux esophagitis (RE) rat, RE rat treated with BR, RE rat treated with 24,30,36,48h GBR). Reflux esophagitis was induced by ligation with a 2-0 silk thread both the pylorus and the transitional junction between the forestomach and the corpus in SD rats.Results： HCl/ethanol-induced gastric mucosal injury mice were ameliorated mucosal damage upon histological evaluation by treatment of 48h GBR than BR. Optical changes such as hyperemia and multiple erosions were observed in the rats with RE and damage to the normal rats was not apparent. The oral administration of GBR significantly diminished against gross mucosal damage in a germination time-dependent manner. Also, the administration of GBR suppressed the biomarker of oxidative stress, reactive oxygen species (ROS) and produces peroxynitrite (ONOO-) in serum. However, the administration of GBR could not affect to the pH level secreted from stomach when compared with Control group.Conclusions： These findings suggest that GBR could have improving effects on upper gastrointestinal diseases in a germination time-dependent manner.

• Research in Plant Disease
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• v.10 no.2
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• pp.112-116
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• 2004

This study was carried out to investigate factors affecting disease development of rice brown spot caused by Cochliobolus miyabeanus in the field and under the greenhouse conditions. In normal paddy fields, the pathogen was more frequently isolated from dried rice straws than wet straws after overwintering. The pathogen was also readily isolated from wet rice straws of salty paddy fields although infection rate of the fields was low. The disease symptom was first observed at 2 to 3days after inoculation when the seedlings were kept for 6 hours in dew chamber. The number of lesions peaked at 14 hr incubation in cultivars Chucheongbyeo and 24 hr incubation in cultivar Daesanbyeo and Tetep. Sixty three-d-old seedlings were more susceptible than 21 to 49-d-old seedlings regardless of cultivar. There was some difference in number of lesions observed, but the lesions were evenly spread regardless of leaf position. Light on the rice seedlings at 3,000 lux or 6,000 lux showed more severe symptoms than those in the dark.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1344-1354
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• 2021

This study was conducted to evaluate the effects of brown rice (Japonica) on growth performance, nutrient digestibility, and blood parameters of weanling pigs. A total of 60 weanling pigs (28-day-old, 30 barrows and 30 gilts, 6.73 ± 0.77 kg body weight [BW]) were randomly allotted to 2 dietary treatments (6 pigs per pen; 5 replicates per treatment) in a randomized complete block design with the initial BW and sex as blocks. The dietary treatments were a typical nursery diet based on corn and soybean meal (CON) and the CON replaced 50% of corn with brown rice (BR). Pigs were fed respective dietary treatments for 5 weeks. For the last week of experiment period, pigs were fed respective dietary treatments containing 0.2% chromic oxide as an indigestible marker. Fecal samples were collected from randomly selected 1 pig in each pen daily for the last 3 d after the 4-d adjustment period. Blood was collected from randomly selected 1 pig in each pen on d 0, 3, 7, and 14 after weaning. Compared with pig fed CON diet, pigs fed the BR diet were found to have higher (p < 0.05) final BW, overall average daily gain, and apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter and energy. However, there were no significant differences between the groups with respect to average daily feed intake, gain to feed ratio, frequency of diarrhea, and the AID and ATTD of crude protein during overall experimental period. Similarly, there were no significant differences on blood parameters between the groups. Thus, the findings of this study indicate that brown rice (Japonica) can be used to replace 50% of corn in the diet of pigs during the nursery period without negatively affecting growth performance, nutrient digestibility, or blood parameters.

• Korean Journal of Poultry Science
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• v.44 no.4
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• pp.235-243
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• 2017

This experiment was aimed at investigating the effects of dietary supplementation with fermented rice bran (FRB) or fermented broken rice (FBR) on egg-laying performance, egg quality, blood parameters, and cholesterol level in egg yolk of Hy-Line Brown egg-laying hens. Altogether, 144 Hy-Line Brown egg-laying hens (32-week-old) were randomly allocated to one of 4 dietary treatment groups, with 4 replicates per treatment. Of them, 3 treatments diets were prepared by supplementing the basal diet with 0.1% probiotics (PRO), 1% fermented rice bran (FRB), or 1% fermented broken rice (FBR) at the expense of corn. Hen-day egg production was higher (P<0.05) in PRO and FRB treatment groups than in the basal treatment groups. However, feed intake, egg weight, egg mass, and feed conversion ratio did not differ among the treatment groups. Additionally, supplementation with FRB or FBR did not affect eggshell strength, eggshell thickness, egg yolk color, and Haugh unit during the feeding trial. There was no significant difference in leukocyte count. Total cholesterol level was lower (P<0.05) in the FRB treatment group than in the basal treatment groups. Asparate aminotransferase, alanine transferase, glucose, and albumin levels were unaffected by dietary supplementation with FRB or FBR. Egg yolk cholesterol level was lower (P<0.05) in the FRB and FBR treatment groups than in the basal treatment groups. In conclusion, dietary supplementation with FRB or FBR improved egg-laying performance, and reduced the levels of total serum cholesterol and cholesterol in egg yolk of Hy-Line Brown egg-laying hens.

• The Korean Journal of Food And Nutrition
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• v.13 no.5
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• pp.411-418
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• 2000

The purpose of this study was to investigate effects of chestnut inner skin tea, brown rice-green tea and Cassia lora tea on the activation of physiological functions (regional cerebral blood flow, mean arterial blood pressure, proliferation of immunocytes in vitro and in vitro, suppression of cancer cell proliferation) in mouse and rat. We used 8 weeks-old balb/c male mice, 300g ICR rats and L1210 cell lines. Regional cerebral blood flow(rCBF) and mean arterial blood pressure(BP) were measured using Leser-Doppler Flowmetry(LDF) and the proliferation of cells was measured using a colorimetric tetrazolium assay(MTT assay). The experimental results are as follows : 1. rCBF was increased by Cassia tora tea, but decreased by chestnut inner skin tea in rats. 2. BP was increased by brown rice-green tea in rats. 3. Proliferation of mouse thymocytes and splenocytes were significantly increased by chestnut inner skin tea in vitro. 4. Proliferation of mouse thymocytes was decreased by Cassia tora tea and brown rice-green tea in vitro. 5. Proliferation of mouse thymocytes was decreased by Cassia tora tea and brown rice-green tea in L1210 transplanted mice. 6. Proliferation of splenocytes was accelerated by chestnut inner skin tea in L1210 transplanted mice. 7. Proliferation of L1210 cells was inhibited by chestnut inner skin tea and Cassia tora tea in L1210 transplanted mice.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.424-431
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• 1996

This study was carried out to provide information for establishing counter measures of soil pollution through analysis of Cd. Cu, Pb, and Zn in paddy soils and brown rice. Cadmium, Cu, Pb, and Zn contents in soils were analyzed and distribution maps for these heavy metals were prepared. Heavy metal contents in brown rice were also measured. Average contents of Cd, Cu, Pb, and Zn in surface paddy soils extracted with 0.1 N HCl were 7.4, 35.8, 98.9, and $118.8mg\;kg^{-1}$, respectively. These were 9 times (Cu) to 50 times (Cd) higher than the background level of heavy metals in unpolluted paddy soils in Korea. The contents of Pb and Zn were lower than those measured in 1980, whereas Cd content did not decrease. The levels of heavy metal contamination in paddy soils may not affect growth or yield of rice plant, however, Cd contents indicated a level of serious concern to humans. The average contents of Cd, Cu, Pb, and Zn in brown rice were 0.38, 2.38, 1.31 and $22.31mg\;kg^{-1}$, respectively.