• KSBB Journal
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• 제13권5호
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• pp.606-614
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• 1998

In this study, biodegradation rate of Arabian light crude oil by mixed cultures of selected petroleum-degraders was determined. Their modes of hydrocarbon uptake were then observed to determine whether there are differences in biodegradation rate by the mixed cultures. By the mixed cultures of petroleum-degraders having same modes of hydrocarbon uptake, such as strain US1 and K1 (using pseudo-solubilized hydrocarbons by a biosurfactants), K2-2 and P1(using hydrocarbons by direct contact), CL 180 and IC-10 (mixed type of uptake modes), the biodegradation rates of aliphatics in the crude oil were increased more than those by their pure cultures, about 40%, 25% and 20%, respectively. Biodegradation rate of strain KH3-2 (using only water- dissolved hydrocarbons) was increased by mixed cultures with strain K1, CL180 or IC-10 possessing high emulsifying activity. However, the biodegradation rate of the crude oil was decreased about 20%-40% by the mixed cultures of petroleum-degraders having different mode of hydrocarbon uptake, such as addition of strain US1 or K1 in the cultures of K2-2 or P1. Biosurfactants produced by US1 or K1 seems to enhance the emulsification of crude oil in aqueous phase but inhibit the attachment of K2-2 or P1 to crude oil. As same phenomena, the addition to Triton X-100 into the culture of strain US1, K1, CL180, IC-10 or KH3-2 increased the biodegradation rate, but the addition in the culture of strain K2-2 or P1 decreased the biodegradation rate. The mixed culture made of CL180, IC-10 and KH3-2 degraded 61.5% of aliphatics and 69% of aromatics in 3% (v/v) of Arabian light crude oil added.

• 한국융합학회논문지
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• 제10권2호
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• pp.41-48
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• 2019

본 연구는 HPMC (Hydroxypropyl methyl cellulose)의 점도와 겉보기 점도가 유탕면의 흡유량과 지방 소화율에 미치는 영향을 확인하고자 하였다. HPMC 상업용 소재의 점도와 밀가루 대체 농도가 증가함에 따라 흡유량 감소와 지방 소화 지연 효과가 나타났다. 한편, 동일한 겉보기 점도를 보이는 수준으로 밀가루 대신 HPMC를 대체하여 제조한 유탕면에서도 겉보기 점도가 같음에도 불구하고 HPMC 자체 점도 증가(높은 중합도를 갖는 시료)에 따른 흡유량 감소와 지방 소화 지연효과를 볼 수 있었다. 이상의 결과로부터, 유탕면의 흡유량 감소와 지방 소화 지연은 겉보기 점도가 동일하다 하더라도 HPMC 자체의 높은 점도가 더 주요한 영향을 주는 것을 확인할 수 있었다.

• Nutrition Research and Practice
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• 제10권5호
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• pp.477-486
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• 2016

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

• 한국환경과학회지
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• 제21권3호
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• pp.369-375
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• 2012

We investigated usefulness of chicken feather as bioadsorbent for removal of hexavalent chromium[Cr(VI)] and oil from aqueous solution. Chicken feather was chemically treated with DTPA, EDTA, NaOH and SDS, respectively. Among them, EDTA was the most effective in adsorbing Cr(VI). Cr(VI) uptake by chicken feather was increased with decreasing pH; the highest Cr(VI) uptake was observed at pH 2.0. By increasing Cr(VI) concentration, Cr(VI) uptake was increased, and maximum Cr(VI) uptake was 0.34 mmol/g. Cr(VI) adsorption by chicken feather was well described by Freundlich isotherm than Langmuir isotherm and Freundlich constant(1/n) was 0.476. As the concentration of chicken feather was increased, Cr (VI) removal efficiency was increased but Cr(VI) uptake was decreased. Most of Cr(VI) was adsorbed at early reaction stage(1 h) and adsorption equilibrium was established at 5 h. On the other hand, chicken feather adsorbed effectively oils including bunker-A and bunker-C. In conclusion, our results suggest that chicken feather waste could be used to remove heavy metal and oil; it is a potential candidate for biosorption material.

• Journal of Ecology and Environment
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• 제37권2호
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• pp.41-51
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• 2014

This study investigated the ability of Bothriochloa bladhii (Retz.) S.T. Blake (Poaceae), Cyperus ligularis L. (Cyperaceae), Commelina erecta L. (Commelinaceae), Mariscus umbellatus (Rottb.) Vahl (Cyperaceae), Fimbistylis miliacea L. (Cyperaceae) and Torulinium odoratum L. (Cyperaceae) to clean up various levels of used motor oil (UMO) contaminated soils. The plants were grown in 2 kg garden soils treated to 0%, 1%, 5% and 10% levels of UMO contamination. The plant growth parameters, chlorophyll contents and dry weight of test plants were measured. The phytoremediation ability of these test plants were assessed by measuring the uptake of hydrocarbons in terms of total hydrocarbon content (THC) as well as their percentage degradation values. There was significant (P < 0.05) reduction in leaf chlorophyll contents and dry weights of the test plant species planted in UMO contaminated soils. THC as well as the percentage uptake (or degradation) of hydrocarbons were both lowest in C. ligularis but highest in T. odoratum in all cases. The phytoremediation potential of test plants was highest in soils contaminated with 5% UMO. Based on the results of this study, all test plants with the exception of C. ligularis were potentially capable of undertaking phytoremediation. However, B. bladhii and T. odoratum proved most effective in the uptake and degradation of UMO.

• 유기물자원화
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• 제28권1호
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• pp.5-14
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• 2020

본 실험은 유박 시용량에 따른 '네로' 블랙초크베리(Aronia melanocarpa)의 양분 요구도를 파악하여 적절한 양분시용량을 구명함으로서 생산비 절감 및 환경오염을 줄이고자 수행되었다. 100% 유박시용을 기준으로 0%(무처리), 25%, 50%, 75%로 분류하여 2년간(2018~2019년) 수체 주위에 매년 전량 산포처리 하였고 실험 조사는 2년차에 수행하였다. 토양 무기성분 농도는 처리구에 상관없이 차이가 없었다. 뿌리와 잎의 건물중은 0% 유박 처리에서 낮게 나타났고 줄기 건물중은 처리 간에 차이가 없었다. 75% 유박 처리에서 과실 건물중이 615 g으로 가장 크게 증가하였고 수량이용효율도 45.3%로 가장 높았다. 지상부:지하부 비율은 75% 처리에서 4.7로 가장 높았다. 유박시용량이 증가할수록 수관용적은 확대되었다. 수관용적은 뿌리 건물중과 유의성 있는 정의 상관관계가 관찰되었다(r²=0.977). 뿌리의 무기성분 흡수량은 철을 제외하고는 25% 유박처리구에서 가장 높았다. 잎은 무처리구에서 전질소와 철의 흡수량이 낮았고 기타 무기성분 흡수량은 유박처리구와 비슷한 수준이었다. 줄기 내 무기성분 흡수량은 50% 처리구에서 철을 제외하고 가장 높았다. 과실 내 무기성분 흡수량은 75% 처리구에서 구리를 제외하고 가장 높았고 특히 전질소 흡수량이 7.45 g으로 다른 기관 대비 가장 높았다. 전질소와 인산흡수량은 잎과 줄기 및 과실에 균일하였고 칼륨은 잎과 과실에 많이 분포하였다. 뿌리, 잎, 줄기, 과실을 합한 작물의 무기성분 총량에서 전질소는 75% 처리구에서 17.2 g으로 가장 높았고 무처리구에서 4.9 g으로 가장 낮았다. 작물체 내 기타 무기성분 흡수량은 50%, 75%, 100% 유박 처리구에서 대체적으로 높은 수준이 관찰되었다.

• The Korean Journal of Ecology
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• 제7권3호
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• pp.170-176
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• 1983

The results obtained from the experiments conducted to investigate Cruciferae plants in rape dependent on the amount of applied fertilizer in aspects of mineral nutrient uptake and on the effects of the composition of oil quality are as follows: Absorption of mineral nutrients in the respective pars of the plant body was high in nitorgen in the order of leaf and stem

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2001년도 Proceedings of the 3rd Annual Meeting of Yellow Sea Environment
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• pp.42-48
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• 2001

A kinetic model was presented to estimate the uptake/release rate constants and thereafter, bioconcentration factor, $k_1$, $k_2$ and BCF (bioconcentration factor), for the uptake of PH by plankton were obtained. Implies that PH(petroleum hydrocarbon) caused no significant influence on the uptake of $N-NO_3$, but significant influence on that of $P-PO_4$. In addition, the application of kinetic model for the bioconcentration of volatile organic toxic compound by organism suggests that the uptake of PH by plankton was an important process for the environmental capacity of PH.

• 산업식품공학
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• 제21권1호
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• pp.72-78
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• 2017

전량 폐기되는 파지두부로부터 제조된 두부 페이스트와 14종의 NSP들을 혼합하여 도넛의 흡유저감에 대한 효과를 조사하였다. 두부 페이스트만을 첨가한 도넛(두부도넛)의 흡유저감율은 10.8%이었으나, 두부 페이스트와 NSP를 함께 사용하는 경우 도넛(NSP-두부도넛)의 흡유저감율은 두부도넛에 비해 향상되었다. 두부 페이스트와 함께 첨가된 NSP들의 흡유저감율에 대한 효과는 NaA가 41.2%로 가장 높았으며, HMP>XAT>CN10T = LMP = ALMP ${\lambda}C$>GG>LBG>${\iota}C$>${\kappa}C$>CN15U>GLG>CN40H 순으로 증가하였다. 또한 NSP 만을 흡유저감 소재로 첨가하였을 때보다 두부 페이스트와 함께 사용하는 것이 도넛의 흡유저감에 더욱 효과적이었고, 그 효과는 음이온성 검류들에서 뚜렷하였다. 유탕처리 시 두부 페이스트의 갈변으로 인해 두부 페이스트 및 두부 페이스트-NSP를 첨가한 도넛들은 대조군에 비해 명도가 감소하고 황색도가 증가하였으나 그 차이는 미미하였다. 두부 페이스트와 함께 NaA, ${\kappa}C$, LBG를 첨가하여 제조된 도넛의 비용적은 대조군과 유사한 수준을 나타내었다. 따라서 도넛의 흡유저감율과 비용적을 고려하면 두부 페이스트와 NaA를 흡유저감 소재로 함께 사용하는 것이 흡유저감 도넛을 제조하는데 가장 적합한 것으로 판단된다. 그럼에도 NaA 보다는 흡유저감율이 적지만 ${\kappa}C$과 LBG을 두부 페이스트와 혼합하여 사용하는 것도 차선책으로서 의미가 있을 것 같다.

• Nutrition Research and Practice
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• 제15권5호
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• pp.568-578
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• 2021

BACKGROUND/OBJECTIVES: Psidium guajava L. (guava) leaves have been shown to exhibit hypoglycemic and antidiabetic effects in rodents. This study investigated the effects of guava leaf extract on adipogenesis, glucose uptake, and lipolysis of adipocytes to examine whether the antidiabetic properties are mediated through direct effects on adipocytes. MATERIALS/METHODS: 3T3-L1 cells were treated with 25, 50, 100 ㎍/mL of methanol extract from guava leaf extract (GLE) or 0.1% dimethyl sulfoxide as a control. Lipid accumulation was evaluated with Oil Red O Staining and AdipoRed assay. Immunoblotting was performed to measure the expression of adipogenic transcription factors, fatty acid synthase (FAS), and AMP-activated protein kinase (AMPK). Glucose uptake under basal or insulin-stimulated condition was measured using a glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose. Lipolysis from fully differentiated adipocytes was measured by free fatty acids release into the culture medium in the presence or absence of epinephrine. RESULTS: Oil Red O staining and AdipoRed assay have shown that GLE treatment reduced lipid accumulation during adipocyte differentiation. Mitotic clonal expansion, an early essential event for adipocyte differentiation, was inhibited by GLE treatment. GLE inhibited the expression of transcription factors involved in adipocyte differentiation, such as peroxisome proliferator-activated receptor 𝛄 (PPAR𝛄), CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein-1c (SREBP-1c). FAS expression was also decreased while the phosphorylation of AMPK was increased by GLE treatment. In addition, GLE increased insulin-induced glucose uptake into adipocytes. In lipid-filled mature adipocytes, GLE enhanced epinephrine-induced lipolysis but reduced basal lipolysis dose-dependently. CONCLUSIONS: The results show that GLE inhibits adipogenesis and improves adipocyte function by reducing basal lipolysis and increasing insulin-stimulated glucose uptake in adipocytes, which can be partly associated with antidiabetic effects of guava leaves.