• Journal of People, Plants, and Environment
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• v.21 no.6
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• pp.485-492
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• 2018

This study aimed to develop and validate highly sensitive determination method of a prostaglandin ($PGE_1$, OP 1206) in human plasma by LC-MS/MS using column switching. Plasma stored at $-30^{\circ}C$ and treated with methanol effectively inhibited interferences synthesized post-sampling. Samples were added with internal standard and were separated by reversed-phase HPLC with a cycle time of 30min. The method was selective for OP 1206 and the regression models, based on internal standard, were linear across the concentration range 0.5-50 pg/mL with the limit of quantification of 0.5 pg/mL (limit of quantitation, LOQ) for OP 1206. The calibration curve of OP 1206 standards spiked in five individual plasma samples was linear ($r^2=0.9999$). Accuracy and precision at the concentrations of 0.5, 1.5, 5.0 and 40 pg/mL, and at the lower LOQ of 0.5 pg/mL were excellent at 20%. OP120 < 6 was stable in plasma samples for at least 24 hours at room temperature, 24 hours frozen at $-70^{\circ}C$, 24 hours in an auto sampler at $6^{\circ}C$, and for two freeze/unfreezing cycles. The validated determination method successfully quantified the concentrations of OP 1206 in plasma samples from simulated administrating a single $5{\mu}g$ OP 1206 formulation. Thus, this novel LC-MS/MS technique for drug separation, detection and quantitation is expected to become the standard highly-sensitive detection method in bioanalysis and to be applied to many low dose pharmaceutical products.

• Journal of the Korean Institute of Gas
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• v.24 no.1
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• pp.17-22
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• 2020

Light Naphtha is distillated from crude oil unit and separated into the methane, ethylene and propylene by boiling point difference in sequence. This separation is conducted using a series of binary-like columns. This separation method is known that the energy consumed in the reboiler is used to separate the heaviest components and most of this energy is discarded as vapor condensation in the overhead cooler. In this study, the first two columns of the separation process are replaced with the Petlyuk column. A structural design was exercised by the stage computation with ideal tray efficiency in equilibrium condition. Compared with the performance of a conventional system of 3-column model, The design outcome shows that the procedure is simple and efficient because the composition of the liquid component in the column tray was designed to be similar to the equilibrium distillation curve. The performance of the new process indicates that an energy saving of 12.1% is obtained and the cost savings of 44 million won per day based on gross domestic product is reduced under same total number of trays and the initial investment cost is saved.

• Journal of Preventive Medicine and Public Health
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• v.40 no.2
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• pp.155-161
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• 2007

Objectives : Bisphenol A diglycidyl ether (BADGE) is the major component in commercial liquid epoxy resins, which are manufactured by co-reacting bisphenol A with epichlorohydrin. This study was performed to show the developmental effects of prenatal and postnatal exposures to BADGE in male rat offspring. Methods : Mated female rats were divided into four groups, each containing 12 rats. The dosing solutions were prepared by thoroughly mixing BADGE in corn oil at the 0, 375, 1500 and 3000 mg/kg/day concentrations. Mated females were dosed once daily by oral gavage on gestation day (GD) 6 - 20 and postnatal day (PND) 0 - 21. Pregnant female dams were observed general symptoms and body weight. Also, male pups were observed the general symptoms, body weight, developmental parameters (e.g. anogenital distance, pina detachment, incisor eruption, nipple retention, eye opening, testis descent), organ pathologic changes and hormone levels of plasma. Results : Pregnant rats treated with BADGE died at a rate of about 70% in the 1500 mg/kg/day group and all rats treated with 3000 mg/kg/day died. Body weight, for male pups treated with doses of 375 mg/kg/day, was significantly lower than in the control group at PND 42, 56, and 63 (p<0.05). Evaluation of body characteristics including; separation of auricle, eruption of incisor, separation of eyelid, nipple retention, descent of testis, and separation of the prepuce in the BADGE treated group showed no difference in comparisons with the control group. AGD and adjusted AGD (mm/kg) for general developmental items in BADGE 375 mg/kg/day treated pups tended to be longer than in controls, however, these differences were not statistically significant. Relative weights of adrenal gland, lung (p<0.05), brain, epididymis, prostate, and testis (p<0.01) were heavier than in control in measures at PND 9 weeks. There were no significant changes in comparisons of histological findings of these organs. Loss of spermatids was observed in the seminiferous tubule at PND 9 weeks, but no weight changes were observed. The plasma estrogen levels were similar in the control and treatment groups at PND 3, 6 and 9 weeks. The plasma testosterone levels in the control group tended to increase with age. However, in the BADGE 375 mg/kg/day treated male pups it did not tend to increase. Conclusions : These findings suggest that BADGE is a chemical that has developmental effects consistent with it being an endocrine disruptor.

• Tribology and Lubricants
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• v.28 no.1
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• pp.33-37
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• 2012

Automotive wheel bearing grease helps to reduce stresses and prevent wear of wheel bearings. But it is easily contaminated by water and other contaminants. Previously, our research group reported the change of grease physical properties such as dropping point, work penetration and oxidation work stability, water washout characteristics, leakage tendency, oil separation, evaporation loss and rust protection by water contamination. In this paper, we analyzed the physical characteristics of grease such as lubricity, viscosity and total acid number to investigate the mechanism of thickener decomposition. In water contaminated grease, the total acid number and wear scar were increased, the viscosity was decreased due to the decomposition of lithium complex thickener.

• Food Science of Animal Resources
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• v.38 no.1
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• pp.26-42
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• 2018

Today incorporation of natural ingredients as inorganic phosphate replacers has come into prominence as a novel research topic due to health concerns about phosphates. In this study, we aimed to investigate the quality of emulsified chicken meatballs produced with Jerusalem artichoke powder (JAP), either alone or in combination with sodium carbonate (SC) as sodium tripolyphosphate (STPP) replacers. The results showed that naturally dried JAP showed favorable technological properties in terms of water-oil binding and gelling. Emulsion batters formulated with JAP-SC mixture showed lower jelly and fat separation, higher water-holding capacity and higher emulsion stability than control samples with STPP. In final product, incorporation of JAP-SC mixture increased moisture and reduced lipid and energy values, and kept the pH value similar to control. Added JAP lead to increments in $b^*$ values whereas decreases $L^*$ values. Cook yield was similar to control in phosphate-free samples formulated with JAP-SC mix. Either low or medium ratios of JAP in combination with SC managed to protect most of the sensory parameters, while sensory scores tend to decrease in samples containing high levels of JAP. Addition of JAP to formulations presented samples that have equivalent behavior to phosphates in terms of lipid oxidation. In conclusion, our study confirms that utilization of JAP in combination with SC had promising effects as phosphate replacers by presenting natural solutions and providing equivalent quality to standard phosphate containing products.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.1
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• pp.12-24
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• 1998

The paper presents the mechanism of secondary flows and the associated total pressure losses occurring in turbine cascades with turning angle of about 127 and 77 degree. Velocity and pressure measurements are taken in seven traverse planes through the cascade passage using a prism type five hole probe. Oil-film flow visualization is also conducted on blade and endwall surfaces. The characteristics of the limiting streamlines show that the three dimensional separation is an important flow feature of endwall and blade surfaces. The larger turning results in much stronger contribution of the secondary flows to the loss developing mechanism. A large part of the endwall loss region at downstream pressure side is found to be very thin when compared to that of the cascade inlet and suction side endwall. Evolution of overall loss starts quite early within the cascade and the rate of the loss growth is much larger in the blade of large turning angle than in the blade of small turning angle.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.2
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• pp.260-269
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• 2000

Oil-film flow visualizations and three-dimensional flow measurements have been conducted in the downstream region of a butterfly-type valve used in air-conditioning systems, with the variation of a disk open angle. The flow visualizations in the flow symmetry plane show that there are a pair of counter-rotating separation/recirculation zones as wall as two jet-like near-wall flows. These flow disturbances are strongly depends on the disk open angle. Based on the flow visualization, a qualitative flow model is suggested in the near-field and downstream region of the valve disk. For a small disk open angle, the mean velocities and turbulent intensities have relatively small values in the near-field of the valve disk, but they do not show uniform distributions even in some downstream region. With an increment of the disk open angle, mean velocity variations and turbulent intensities are greatly increased in the immediate downstream region, but uniform distributions are quickly resumed as departing from the valve disk. The mass flow rate remains nearly constant for the disk open angles less than 30 degrees, meanwhile it strongly depends on the disk open angles between 45 and 75 degrees. The pressure loss is found to be about zero for the disk open angles less than 45 degrees, but is substantially increased for those larger than 75 degrees.

• The KSFM Journal of Fluid Machinery
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• v.12 no.1
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• pp.35-42
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• 2009

The flow and heat/mass transfer characteristics on the squealer tip surface of a high-turning turbine rotor blade have been investigated at a Reynolds number of $2.09{\times}10^5$, by employing the oil-film flow visualization and naphthalene sublimation technique. The squealer rim height-to-chord ratio and tip gap height-to-chord ratio are fixed as typical values of $h_{st}/c$ = 5.5% and h/c = 2.0%, respectively, for turbulence intensities of Tu = 0.3% and 15%. The results show that the near-wall flow phenomena within the cavity of the squealer tip are totally different from those over the plane tip. There are complicated backward flows from the suction side to the pressure side near the cavity floor, in contrast to the plane tip gap flows moving toward the suction side after flow separation/reattachment. The squealer tip provides a significant reduction in tip surface thermal load with less severe gradient compared to the plane tip. In this study, the tip surface is divided into six different regions, and transport phenomena at each region are discussed in detail. The mean thermal load averaged over the squealer cavity floor is augmented by 7.5 percents under the high inlet turbulence level.

• Journal of the Korean Institute of Gas
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• v.11 no.1 s.34
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• pp.18-22
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• 2007

The fault current through the earth originated from a power line ground fault might cause arcing through the soil to an adjacent pipeline, which might bring about not only a catastrophic accident such as gas explosion and oil leakage but also a hazard to the safety of workers responsible for the maintenance and repair of the pipeline. In this paper we investigated the arcing phenomena through soil between a power line tower and a pipeline and outlined the standards for the separation distance of a buried pipeline adjacent to the power line tower.

• Journal of Nutrition and Health
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• v.11 no.1
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• pp.9-16
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• 1978

A Comparison of the analysis of the green and roast coffee of Arabica and Robusta compositions and regular instant coffee has been investigated by chromatography. The coffee oil were obtained by extracting the green and roast coffee with ethyl ether by soxhlet methood. Instant coffee samples were accurately weighted into 100ml beaker (ca. 0.5g regular coffee and 1.5g decaffeinated coffee) and add ca. 50ml water, heat and boil, remove from heat, and mechanically stried ca. 15min. and filtered of one sample and another sample were without filtrated and proceed with liquid chromatographic separation. The fatty acid compositions of green and roast coffee were compared by gas liquid chromatography and general chemical compositions of sample were analysed. Some similarities between green and roast coffee fatty acids were found in the case of green and roast coffee of both kinds acid methyl esters. They contained stearic, oleic, linoleic, and unknown fatty acid, and palmitic ana linoleic acid were rich.