• 동의생리병리학회지
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• 제21권5호
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• pp.1291-1296
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• 2007

The expressions of c-Fos and nitric oxide synthase (NOS) represent neuronal activity and play' a crucial role in the shaping of the development of brain. During the late pregnancy, stresses may influence neuronal activity of prenatal rats. In the present study, the effects of prenatal noise and music on the expressions of c-Fos and NOS in the hippocampus of rat pups were investigated. Exposure to the noise during pregnancy decreased c-Fos and NOS expressions in the hippocampus of rat pups, whereas exposure to music during pregnancy increased c-Fos and NOS expressions in the hippocampus of rat pups. The present results show that prenatal music stimulation may increase neuronal activity of rat offspring, whereas exposure to noise during pregnancy may reduce the neuronal activity of offspring. The present study suggests that prenatal stimuli including noise and music could affect the fetal brain development.

• The Korean Journal of Ecology
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• 제28권5호
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• pp.255-260
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• 2005

We investigated interventions by mother Jeju ponies on Jeju Island, Korea, to determine whether mothers assisted their offspring to attain higher status within the dominance hierarchy. Because dominance rank is important within each gender, we predicted that mothers would be more likely to intervene when their foals were play-fighting with foals of the same gender. A total of 173 play-fighting events were recorded from March to October 1998 and from April to October 1999. Of these, foals were more likely to play-fight with a foal of the same gender as with a foal of the opposite gender (120 versus 53 occurrences, respectively). A mother of one of the foals that were play-fighting intervened in 17 of these interactions. Contrary to the prediction, a mare was more likely to intervene when opposite genders interacted than when the same gender interacted. Analyzing interactions between the opposite genders further, mothers were equally likely to intervene when a daughter was play-fighting with a male foal as when a son was play-fighting with a female foal. Hence, mothers were not more protective of daughters than sons. Mothers that were in the younger age class ($2\sim11$ years old) were as likely to intervene as those in the elder age class ($17\sim25$ years old). However, all foals that were harassed were offspring of mothers in the younger, more subordinate age class. intervention directly maintains the dominance rank of the intervening mother, and may indirectly assist the intervening mother's foal to achieve a higher dominance rank. By discouraging their foals from play-fighting with the opposite genders, dominant mothers may be encouraging their foals to play-fight with the same gender and participate in establishing its own dominance rank.

• Journal of Korean Neurosurgical Society
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• 제58권1호
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• pp.14-21
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• 2015

Objective : To study the effect of early intervention and rehabilitation in the expression of aquaporin-4 and ultrastructure changes on cerebral palsy pups model induced by intrauterine infection. Methods : 20 pregnant Wistar rats were consecutively injected with lipopolysaccharide intraperitoneally. 60 Pups born from lipopolysaccharide group were randomly divided into intervention group (n=30) and non-intervention group (n=30); intervention group further divided into early intervention and rehabilitation group (n=10), acupuncture group (n=10) and consolidate group (n=10). Another 5 pregnant rats were injected with normal saline intraperitoneally; 30 pups born from the normal saline group were taken as control group. The intervention group received early intervention, rehabilitation and acupuncture treatment. The motor functions of all pups were assessed via suspension test and modified BBB locomotor score. Aquaporin-4 expression in brain tissue was studied through immunohistochemical and western-blot analysis. Ultrastructure changes in damaged brain and control group were studied electron-microscopically. Results : The scores of suspension test and modified BBB locomotor test were significantly higher in the control group than the intervention and non intervention group (p<0.01); higher in the intervention group than the non-intervention group (p<0.01). The expression of Aquaporin-4 was lower in intervention and non intervention group than in the control group (p<0.01); also lower in non-intervention group than the intervention group (p<0.01). Marked changes were observed in ultrastructure of cortex and hippocampus CAI in brain damaged group. Conclusion : Early intervention and rehabilitation training can improve the motor function in offspring with brain injury and reduce the expression of aquaporin-4 in damaged brain.

• 한국가금학회지
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• 제26권2호
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• pp.119-129
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• 1999

In recent years, numerous researches have been carried out in author's laboratory to develop several kinds of methods for producing transgened chicken, leaving a lot of new findings. Some of them are very useful to search for new approaches necessary to improve the efficiency of hatchability and the survival rate of developing trasgened embryos. The results obtained hitherto might be summarized as follows: (1) foreign gene(Lac Z/ Miw Z) introduced into blastodermal cells of developing embryos was successfully transferred to embryos, leading to the production of primordial germ cells(PGCs) carrying foreign DNA. However, hatched hickens failed to show the incorporation of introduced gene into the gonads. (2) When foreign gene was introduced into germinal crescent region (GCR), the gene was also efficiently incorporated into germ cells, resulting in the production of transgened chickens(offspring) which produced fruther offspring having foreign gene in the gonads. In this case, 2nd and 3rd generations of chickens were obtained through the reproduction of transgened birds. (3) In another way, the gene was injected into blood vessels of developing embryos at stage 13∼15, creating PGCs having foreign gene, and produced some transgened chickens. In this work, the PGCs were transfered between embryos, resulting in the production of transgenic chickens. (4) in these experiments, PGCs were effectively employed for producing transgenic birds, developing some kinds of chimeric chickens from homo- or hetero-sexual transfer of the PGCs from embryos. This means that the gonads from donor PGCs developed in some degree to the stage of hatching. However, these gonads showed slightly abnormal tissues similar to ovotestis like organs through histological examination. (5) Avian Leukosis Virus(ALV) induced B cell line(DT40) successfully carried foreign genes into chicken embryos, suggesting the possibility of the cells as a vector in this field of study in the future. (6) Inter-embryonic transfer of the PGCs also gave us some.

• Parasites, Hosts and Diseases
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• 제23권1호
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• pp.151-155
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• 1985

CGVS 배지에서 무균 배양한 Naegleria fowleri 영양형을 5% formaldehyde로 고정시켜 $1{\times}10^6$개씩 일주일 간격으로 3회 또는 6회 BAKA/c 마우스 복강내에 주입시켜 면역시켰다. 면역이 끝나고 7일후 배양된 N. fowleri영양형 $5{\times}10^4$개를 secobarbital로 마취된 마우스 비강에 떨어뜨려 감염시켰다. 어미 마우스에만 또는 어미와 그 새끼 마우스에 모두 면역시켰을 때, N. fowleri 감염에 의한 사망율에 있어 대조군과 차이가 없었으나 그 생존기간은 연장됨을 알 수 있었다. 그러나 새끼마우스에만 면역시켰을 때 사망율이 현저히 감소하였고 생존기간도 훨씬 연장되었다. 새끼 마우스에만 면역시켰을 때 방어면역 효과가 현저하였으나, 어미 마우스에만 또는 면역시킨 어미로부터 태어난 새끼 마우스에 면역시켰을 때는 방어면역이 나타나지 않음을 알 수 있었다.

• 한국가축번식학회지
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• 제27권4호
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• pp.317-324
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• 2003

The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F$_{0}$). The first generation of transgenic pig (F$_{0}$) harboring mWAP-hEPO appeared to be a male, and the second generation (F$_1$) pigs were made by natural mating of F$_{0}$ with domestic swine, and male and female transgenic pigs (F$_1$) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.earch.

• Current Research on Agriculture and Life Sciences
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• 제8권
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• pp.51-58
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• 1990

본(本) 연구는 micromanipultor를 이용하여 생쥐의 8세포기배와 상실배 그리고 배반포기배를 양분(兩分)후 생존성을 검토하고, 또한 배반포기배를 양분후, 선별(選別)및 배양 과정없이 암컷 생쥐에 이식(移植)하는 경우 새끼쥐 생산의 가능성을 검토하고자 수행된 것이다. 그 경과를 요약해 보면 다음과 같다. 1. 생쥐의 8세포기배(細胞期胚)와 상실배(桑實胚)를 양분(兩分)하여 M2에 배양한결과 각각 64%, 81%가 배반포기배(胚盤胞期胚)까지 발생(發生)하였다. 2. M2 배양액에서 발생시킨 배반포기배를 Ham's F-10에서 배양한 결과 8세포기배에서는 86%, 상실배에서는 90%가 정상적으로 outgrowth 되었다. 3. 배반포기배를 배양 과정없이 바로 양분(兩分)하여 Ham's F-10에서 배양한 결과 97%가 정상적으로 outgrowth 되었으나 암컷생쥐에 이식한 결과 산차는 얻지 못하였다.

• 한국지능시스템학회논문지
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• 제22권4호
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• pp.527-531
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• 2012

본 논문에서는 종래의 PSO 알고리즘 성능저하의 주요 원인들 중 하나인 입자들의 조기수렴 현상을 개선한 DPSO-QI (Distributed PSO with quantum-infusion mechanism) 기법을 제안한다. DPSO-QI 알고리즘은 다음과 같은 두 가지 특징을 지닌다. 첫째, 분산형 구조의 PSO 기법을 도입한다. 이는 먼저 적절한 수의 입자들로 소그룹을 형성하고, 최적해 탐색에 필요한 다양한 정보의 교환이 각 소그룹 내에서만 이루어지도록 한 기법이다. 이러한 기법을 바탕으로 입자들의 탐색 다양성을 증대시킴으로서 조기수렴 현상을 감소시키는 효과를 달성할 수 있다. 둘째, 상기의 입자 소그룹에 Quantum-infusion (QI) 메커니즘에 기반 한 기법을 도입시킨다. 이를 통해 입자들의 전역 최적해 탐색 정밀도를 보다 향상시킬 수 있다. 끝으로 다양한 수치예제를 통하여 제안하는 새로운 PSO 기법이 종래의 방식들에 비해 매우 뛰어난 성능을 구현할 수 있음을 입증하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.435-439
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• 2000

Records of weight, age, body condition at calving and udder characteristics scores of 425 beef cows and birth weights and periodical weights of their offspring from birth to weaning were analyzed to study the effect of body weight, condition and udder characteristic scores of dams on birth weight and Preweaning growth of their offspring. Dam's body condition (fat reserve) at calving were scored on a scale of 1 to 5 (1=extremely thin and 5=extremely fat) and dam's udder characteristics were scored on a scale of 1 to 7 (l=udder well attached with small teats and 7=at least one quarters not functional). Dams were from three distinct breed groups and were mated in single sire mating groups within each breed group for 45 days. Within each breed group and year, the dams were classified into high, medium and low based on their weights at the time of parturition. The data were analysed using covariance analysis. In general, calves born to heavier dams were heavier at birth and had higher rate of Preweaning growth. The effect of dam's body condition score on the calf birth weight was not significant. However, cows with average body condition score of 2.5 or 3.0 gave birth to calves that had higher pre weaning growth rates up to weaning than those born to calves with higher body condition score. The udder characteristics score did not affect calf birth weight as expected; however, cows with udder score of 3 (udder well attached with large teats) produced calves with higher preweaning growth rate than those with smaller teats. Based on the results, it can be concluded that maintaining animals with average body condition and weight would result in more efficient calf production. In addition, cows with well attached udder and large teats would provide a better maternal environment for Preweaning growth rates of their calves.

• Journal of Preventive Medicine and Public Health
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• 제19권1호
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• pp.100-122
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• 1986

Obtaining an available information on health concerns of parents of school-age children and furthermore developing the sound policy for the public on health education, this study was conducted during Nov.-Dec. 1985 in the four selected areas; Seoul, large, middle and small city, and farming and fishery villages for 3,337 parents of schoolchildren in Korea. In this study, parents complected a questionnaire containing 34 items related to health concerns(e.g. drinking alcohol, air pollution, cancer, etc. see Table 4 and Fig. 2). For each health items, respondents were asked to indicate a choice between three levels of concern; 'Very high concern,' 'Moderate concern,' 'Little concern', and 'No opinion'. An analysis of responses indicated that most of the top ten health concerns identified by parents as cancer, abortions among high school students, medical malpractice, water pollution, traffic accidents, air pollution, suicide of parents and homicide of offspring, heart disease, venereal diseases, and high blood pressure in that order. Those health problems about which respondents were least concerned were more closely related to the individual, such as drinking alcohol, smoking, tooth decay, gum disease, underweight, overweight. Of greater concern were more such as water pollution, abortions among high school students, air pollution, accidents, medical malpractice. For cancer, heart disease, high blood pressure, various accidents, environmental pollution, parents showed high concern, however, for health issues which contributed as causative influences such as lung cancer from smoking and liver cirrhosis and traffic accidents from drinking alcohol showed less concern. Relationship between parent's residential areas, educational level and sex distribution and health concern showed little difference, however, for these issues parents identified as relatively high concern. Most parents stowed more concern in sex-related of family-related health issues such as abortions among high school students, suicide of parents and homicide of offspring.