• 생명과학회지
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• 제19권6호
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• pp.711-719
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• 2009

본 연구는 2006년 8월부터 2007년 9월까지 여수, 남해, 진도, 무안, 거문도, 서산 및 중국의 산동에서 포획한 낙지 유전자 집단을 분석하기 위하여 미토콘드리아 16S rRNA 염기서열로 조사했다. 유전자 분석은 총 28 개체로부터 11개의 haplotype이 발견되었다. 유전자 분화율은 0.2-1.2% 범위로 나타났다. Haplotype에 대한 PHYLIP 및 network 조사에 따르면 낙지는 두개의 clade (clade AIclade B)로 나뉘어지며, clade 사이의 분화율은 0.4%로 나타났다. 지역적 거리에 따라 haplotype이 다음과 같이 분화되었다. 하나는 여수, 남해, 무안, 진도 haplotype과 다른 하나는 서산, 거문도, 산동 haplotype으로 나뉘어졌다. 계충구조 분석에서도 한국 낙지집단 및 중국과의 유전적 차이를 볼 수 있으나, 현저한 지역적 차이는 나타나지 않았다. 따라서 한국연안에 서식하고 있는 일부 낙지집단은 gene flow에 의해서 유전적 동질성을 나타낼 수 있지만, 한국집단 간 뿐만 아니라 중국집단과의 유전적 분화는 지역적 거리 및 장벽으로 인하여 제한적인 gene flow로 설명될 수 있다.

• Applied Microscopy
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• 제32권2호
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• pp.175-183
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• 2002

한국 연근해산 살오징어와 서해낙지의 시엽에서 신경전달물질을 분비하는 neuron의 특성 및 기능을 확인하기 위해 rabbit anti-dopamine (TH)과 rabbit anti-calbindin-$D_{28K}$ 등의 항체를 이용한 면역염색과 면역금지법을 시행한 결과는 다음과 같았다. 항-dopamine을 사용한 면역염색 결과 살오징어에서는 외과립세포층 상단에 위치한 대형 무축삭세포와 수질부의 섬을 이루는 세포들 중 $2{\sim}3$개의 비교적 소량의 세포만이 양성반응을 보인 반면, 서해낙지에서는 외과립세포층 상단 및 중간 부위의 $2{\sim}3$개의 세포와 수질부섬을 이루는 5개 이상의 세포에서 강한 양성반응을 보였다. 항-calbindin-D28k인 경우는 살오징어의 외과립세포층 상단에 위치한 $2{\sim}3$개의 소형 무축삭세포와 내과립세포층 하단에 위치한 $1{\sim}2$개의 세포에서 양성반응을 보인 반면, 서해낙지인 경우는 외과립세포층에서 4개 정도의 세포에서 양성 반응을 보였으나, 내과립세포층에서는 반응을 확인할 수 없었다. 면역금지법을 시행한 결과 살오징어에서는 항-dopamine과 항-calbindin에 면역반응을 보인 세포들의 세포질 $0.5{\mu}m^2$당 $17{\sim}26$개 정도의 비교적 많은 수의 금입자가 표지된 반면, 서해낙지에서는 세포질 $0.5{\mu}m^2$당 평균 10개 정도인 소량의 금입자만이 표지되어, 살오징어가 서해낙지에 비해 표지된 금입자가 거의 2배 정도 많았다.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.111-117
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• 2016

Vagal nerve activity has been known to play a crucial role in the induction and maintenance of atrial fibrillation (AF). However, it is unclear how the distribution and concentration of local acetylcholine (ACh) promotes AF. In this study, we investigated the effect of the spatial distribution and concentration of ACh on fibrillation patterns in an in silico human atrial model. A human atrial action potential model with an ACh-dependent $K^+$ current ($I_{KAch}$) was used to examine the effect of vagal activation. A simulation of cardiac wave dynamics was performed in a realistic 3D model of the atrium. A model of the ganglionated plexus (GP) and nerve was developed based on the "octopus hypothesis". The pattern of cardiac wave dynamics was examined by applying vagal activation to the GP areas or randomly. AF inducibility in the octopus hypothesis-based GP and nerve model was tested. The effect of the ACh concentration level was also examined. In the single cell simulation, an increase in the ACh concentration shortened $APD_{90}$ and increased the maximal slope of the restitution curve. In the 3D simulation, a random distribution of vagal activation promoted wavebreaks while ACh secretion limited to the GP areas did not induce a noticeable change in wave dynamics. The octopus hypothesis-based model of the GP and nerve exhibited AF inducibility at higher ACh concentrations. In conclusion, a 3D in silico model of the GP and parasympathetic nerve based on the octopus model exhibited higher AF inducibility with higher ACh concentrations.

• 한국수산과학회지
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• 제39권5호
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• pp.410-418
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• 2006

Sexual maturity and growth characteristics of Octopus minor were investigated in 796 individuals from mud flats in Goorori, Muan-Goon, Korea. Gonadosomatic index (GSI) peaked between June and July in females and between November and January in males. The discrepancy in the index peaks between females and males might result from the earlier sexual maturation of males. The sex ratio was biased toward females (68%) in April and toward males (78%) in September, although the differences were not significant (p>0.05). In females, about 50% of individuals with a mantle length of 70.6 mm were sexually mature versus 100% of individuals with mantle lengths over 80 mm. In females, fecundity was also related to size, ranging from 44 eggs in individuals with a mantle length of 54 mm to 179 eggs in the female with a mantle length of 100.5 mm. The relationship between mantle length (ML) an body weight (BW) was BW=0.008 ML$^{2.2797}$ (n=389, r$^2$=0.83, p<0.01) in males and BW=0.029 ML$^{2.2797}$ (n=407, r$^2$=0.74, p<0.01) in females. Analysis of co-variation showed that the difference in the slopes of the two regression lines was significant (p<0.01). Analysis of mantle length-frequency for each month revealed 1 to 3 normal distribution modes. The growth parameters obtained by fitting the modal progression to the seasonalized von Bertalanffy growth function (VBGF) were ML$\infty$= 112.38 mm, K=1.9, C=0.90, and WP=0.1. The results indicate that the seasonal growth oscillation of the stock is very strong and winter is the season when growth is the slowest.

• Preventive Nutrition and Food Science
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• 제18권4호
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• pp.273-279
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• 2013

Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease was obtained from the strain Planomicrobium sp. L-2 through ammonium sulfate precipitation, dialysis and enrichment, DEAE-Sephadex A50 anion-exchange chromatography, and Sephadex G-100 gel chromatography. The molecular weight and properties of the protease were characterized, including optimum temperature and pH, thermal stability, protease inhibitions and metal ions. According to our results, the protease from Planomicrobium sp. L-2 strain designated as F1-1 was obtained by three-step separation and purification from crude enzyme. The molecular weight of the protease was 61.4 kDa and its optimum temperature was $40^{\circ}C$. The protease F1-1 showed a broad pH profile for casein hydrolysis between 5.0~11.0. No residual activity was observed after incubation for 40 min at $60^{\circ}C$ and 60 min at $50^{\circ}C$. F1-1 protease was inhibited by $Mn^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ ions, as well as PMSF, indicating that the protease F1-1 was a serine protease. Additionally, research basis provided by this study could be considered for industrial application of octopus intestinal proteases.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.989-1002
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• 2022

Cephalopods, in particular octopus (Octopus vulgaris), have the ability to alter their appearance or body pattern by showing a wide range of camouflage by virtue of their chromatophores, which contain nanostructured granules of ommochrome pigments. Recently, the antioxidant and antimicrobial activities of ommochromes have become of great interest; therefore, in this study, the pH-dependent redox effect of the extraction solvent on the antioxidant potential and the structural characterization of the pigments were evaluated. Cell viability was determined by the microdilution method in broth by turbidity, MTT, resazurin, as well as fluorescence microscopy kit assays. A Live/Dead Double Staining Kit and an ROS Kit were used to elucidate the possible inhibitory mechanisms of ommochromes against bacterial and fungal strains. The results obtained revealed that the redox state alters the color changes of the ommochromes and is dependent on the pH in the extraction solvent. Natural phenoxazinone (ommochromes) is moderately toxic to the pathogens Staphylococcus aureus, Bacillus subtilis, Salmonella Typhimurium and Candida albicans, while the species Pseudomonas aeruginosa and Pseudomonas fluorescens, and the filamentous fungi Aspergillus parasiticus, Alternaria spp. and Fusarium verticillioides, were tolerant to these pigments. UV/visible spectral scanning and Fourier- transform infrared spectroscopy (FTIR) suggest the presence of reduced ommatin in methanol/ HCl extract with high intrinsic fluorescence.

• 문화기술의 융합
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• 제9권1호
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• pp.135-140
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• 2023

VR애니메이션은 애니메이션 속 공간에 위치하면서 기존 애니메이션 연출에서 볼 수 없는 360도 화면 연출을 볼 수 있다. 2020년에 제작된 VR 애니메이션 <Goodbye Mr Octopus>는 몰입형 단편 영화로 제77회 베니스 영화제에서 선정되었다. 열여섯 번째 생일을 맞은 사춘기 소녀 스텔라와 엄격한 아버지와의 갈등을 어머니의 편지로 해결되는 이야기이다. 총 11개의 씬으로 구성된 서사로 각 씬에는 시선유도, 시간흐름, 공간전환, 외화면 연출 등 VR영상 문법의 새로운 연출적 요소가 분석되었다. 시선유도 연출은 360도 시선의 불편함을 최소화하였으며, 시간 및 공간전환 연출은 서사적 사건에 따른 관객의 몰입도를 높이고, 외화면 연출은 VR공간의 확장 효과로 분석되었다.

• 수산해양기술연구
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• 제13권2호
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• pp.27-32
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• 1977

The paper deals the fishing condition of the stern trawlers operated in the Atlantic coast of Africa. The datas are gathered from the Korean stern trawlers operated in the area from June, 1975 to May, 1976. The obtained results are summarized as follows: 1. The mean catch per haul are calculated as, cuttle fish 14.5kg, large size squid(more than :lOOg of body weight) 28.2kg, small size squid(less than 300g) 36.4kg, octopus 47.0kg. 2. Small size cuttle fish(less than5OOg of body weight) are caught much during after spring tide in July to September, yet, large size ClIttle fish(more than 500g) are caught much during neap tide in October to January. 3. Small size squid(less than 300g of body weight) are cBught much during after spring tide in October to December, yet, large size squid(more than 3OOg) are caught much during before spring tide in October to March. 4. Octopus are caught during neap tide in July to August.

• 수산해양기술연구
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• 제43권4호
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• pp.339-346
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• 2007

Estimating fishing capacity is one of current hot issues in the international fisheries. It is because that increased fishing capacity has caused not only fish stocks to be reduced, but also additional fishing costs to be incurred without additional incomes, which resulted in decrease of economically viability of fisheries. In order to solve this problem, FAO adopted 'the International Plan of Action for the Management of Fishing Capacity' in 1999 and recommended that member countries to measure fishing capacity and to implement the domestic action plan to reduce excess fishing capacity. This study is aimed at assessing fishing capacity of the octopus coastal trap fishery(OCTF) using data envelopment analysis(DEA) which is a method recommended by FAO. The DEA results on 10 individual OCTF vessels showed that the capacity utilization(CU) was a 0.93 on average, indicating some differences in CU among vessels(0.79-1.0). In addition, results of the sensitivity analysis revealed that under the current level of catch, the gross tonnage, horse power, days fished, and traps per trip could be reduced by 35%, 33%, 16%, and 18% on average, respectively.

• 수산경영론집
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• 제46권3호
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• pp.31-49
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• 2015

This study examines whether or not the South Korean major fishery product import markets; Frozen Pollock, Frozen Long Arm Octopus, and Frozen Hairtail are integrated. We are utilizing the Multivariate and Bivariate Johansen Co-integration test to see if the law of one price(LOP) holds in each market or not. The empirical results show that even though import prices from different countries affect each other in each South Korean major fishery product import market, there is no evidence of LOP in any fishery product import market, which means that none of the markets are integrated. Based on these results, we could expect that the three major fishery product import markets show monopolistic competition among import countries. we would also see whether or not any country plays the role of a price leader in any of the markets. Based on weak exogeneity test results, we might expect that the United States and Malaysia are price leaders in the South Korean Frozen Pollock Import Market and Frozen Long Arm Octopus Import Market, respectively; however, we need to study more on this in the future.