• 제목/요약/키워드: observation fusion

검색결과 141건 처리시간 0.028초

Magnetic Properties of Modified DNAs

  • Do, Eui-Doo;Lee, Chang-Hoon;Kwon, Young-Wan;Choi, Dong-Hoon;Jin, Jung-Il;Oh, Dong-Keun;Nishide, Hiroyuki;Kurata, Takashi
    • 한국고분자학회:학술대회논문집
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    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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    • pp.3-4
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    • 2006
  • Natural DNAs in dry state, i.e., A-DNAs, when intercalated with low levels of stable organic free radicals or complexed with low levels of Au(III), are attracted at room temperature to commercial magnets, whereas those containing high levels of intercalators or Au(III) are not. This surprising observation is explained by the EPR spectra and SQUID measurement of magnetization of the modified DNAs. It is conjectured that A-DNAs are morphologically heterogeneous containing ordered and disordered regions. The ordered regions appear to strongly mediate magnetic interactions between spins through their ${\pi}_z$-stacked structures. When the modified DNAs are wet or hydrated, they behave diamagnetically.

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Dynamics of OH Production in the Reaction of O(1D2) with Cyclopropane

  • Jang, Sungwoo;Jin, Sung Il;Kim, Hong Lae;Kim, Hyung Min;Park, Chan Ryang
    • Bulletin of the Korean Chemical Society
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    • 제35권6호
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    • pp.1706-1712
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    • 2014
  • The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).

Interaction of Heliothis armigera Nuclear Polyhedrosis Viral Capsid Protein with its Host Actin

  • Lu, Song-Ya;Qi, Yi-Peng;Ge, Guo-Qiong
    • BMB Reports
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    • 제35권6호
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    • pp.562-567
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    • 2002
  • In order to find the cellular interaction factors of the Heliothis armigera nuclear polyhedrosis virus capsid protein VP39, a Heliothis armigera cell cDNA library was constructed. Then VP39 was used as bait. The host actin gene was isolated from the cDNA library with the yeast two-hybrid system. This demonstrated that VP39 could interact with its host actin in yeast. In order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pEGFP39. The fusion protein was expressed in the Hz-AM1 cells under the control of the Autographa californica multiple nucleopolyhedrovirus immediate early gene promoter. The host actin was labeled specifically by the red fluorescence substance, tetramethy rhodamine isothicyanete-phalloidin. Observation under a fluorescence microscopy showed that VP39, which was indicated by green fluorescence, began to appear in the cells 6 h after being transfected with pEGFP39. Red actin cables were also formed in the cytoplasm at the same time. Actin was aggregated in the nucleus 9 h after the transfection. The green and red fluorescence always appeared in the same location of the cells, which demonstrated that VP39 could combine with the host actin. Such a combination would result in the actin skeleton rearrangement.

API X-100의 레이저-아크 하이브리드 용접성에 미치는 용접와이어의 영향 (The Effects of Welding Wires on the Weldabilities of API X-100 with Laser-Arc Hybrid Welidng)

  • 김성욱;이목영
    • Journal of Welding and Joining
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    • 제32권5호
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    • pp.7-12
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    • 2014
  • In this study, API-X100 steel pipes were welded with various kinds of welding wires in the laser-arc hybrid welding process. 10kW fiber laser source was combined to MIG arc welding process. API X-100 steel of base metal was of 16.9mm thickness, and butt welding applied. After welding, full penetration weld was acquired by 1-pass welding. A root porosity and the lack of fusion was observed in some welding conditions. By the mixing the melted wire, acicular ferrite, polygonal ferrite, pro-eutectoid, aligned side plate, and bainite structures were observed at the weld metal. From the observation of hybrid weld, unmixed zone had more Ni and Cr. The unmixed zone was a 1/3 area of the weld metal. As the mechanical test of the hybrid welding, tensile test and impact test applied. From the tensile test, all of the welding except SM70S was fractured at the base metal. The result of the impact test at -30 degree C led 60J~320J of the absorbed energy. The result of the low-absorbed energy might be from the coarse equiaxed structures of the weld metal.

Adaptive Optics in Institute of Optics and Electronics, China

  • Jiang, Wenhan;Ling, Ning
    • 한국광학회:학술대회논문집
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    • 한국광학회 2000년도 하계학술발표회
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    • pp.3-3
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    • 2000
  • Adaptive Optical (AO) technology can compensate for wave-front errors in real-time to improve image and beam quality. The research and development on AO in China began in 1979. In 1980, the first laboratory on AO in China was established in Institute of Optics and Electronics (IOE), Chinese Academy of Sciences (CAS). Since then several AO systems have been built in this Laboratory. The 19-element system is the first AO system in the world ever used in inertial confinement fusion (ICF) facility in our knowledge. It corrects the static error of this large laser engineering. The 21-element system was firstly tested at the 1.2m telescope of Kunming Observatory in 1990 and then up-dated as an IR AO system installed at the 2.16m telescope of Beijing Observatory. The 37-element system was used with a turbulence cell in Laboratory on Atmospheric Optics in Hefei to conduct elementary research on Atmospheric Optics. The 61-element system for astronomical observation is newly developed. It has been successfully installed at the 1.2m telescope of Kunming Observatory and a laser guide star system will be integrated with the system. A compact AO system using our newly developed miniature DM for high resolution ophthalmic imaging of retina is also being built. The key elements of these AO systems, deformable mirrors and fast-steering mirrors, are all developed in this Laboratory. In this talk, the main configurations of these AO systems, some test results as well as the specifications of these active mirrors will be presented.

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연속파 Nd:YAG 레이저를 이용한 치아교정 급속 구개확장장치 용접특성 (Welding Characteristics of Rapid Palatal Expander for Teeth Calibration using a Continuous Wave Nd:YAG Laser)

  • 유영태;양윤석;신호준
    • 한국정밀공학회지
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    • 제27권2호
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    • pp.40-49
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    • 2010
  • The Purpose of this paper is to weld a rapid palatal expander using a continuous wave Nd:YAG laser. The rapid palatal expander has become a useful treatment method for severe maxillary transverse deficiencies and posterior crossbites. Rapid maxillary expansion is a well-established method to correct transverse maxillary deficiency and arch length discrepancy. The major process parameters studied in the present laser welding experiment were the positions of focus, laser power and travel speed of laser beam. We measured the fusion zone size and its shape using an optical microscope for the observation of cross-sectional area and tension stress of a rapid palatal expander welded. Through the experimental investigation, the optimum speeds and power of laser without deficiencies of weld cross-sectional area were obtained.

Regulation of the sufABCDSE Operon by Fur

  • Lee, Joon-Hee;Yeo, Won-Sik;Roe, Jung-Hye
    • Journal of Microbiology
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    • 제41권2호
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    • pp.109-114
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    • 2003
  • A promoter that is inducible by paraquat and menadione, the superoxide generators, independently of soxRS has been found in front of the sufABCDSE operon in Escherichia coli. Based on the observation that SufA is a holomog of IscA that functions in the assembly of iron sulfur cluster and the sufA promoter (sufAp) contains a putative Fur-binding consensus, we investigated whether this gene is regulated by Fur, a ferric uptake regulator, When examined in several sufAp-lacZ chromosomal fusion strains, sufAp was induced by EDTA, an iron chelator and a well-known Fur-inducer, The basal level of sufA expression increased dramatically in fur mutant, suggesting repression of sufAp by Fur. The derepression in fur mutant and EDTA-induction of sufA expression required nucleotides up to -61, where a putative Fur box is located. Purified Fur protein bound to the DNA fragment containing the putative Fur box between -35 and -10 promoter elements. The regulation by Fur and menadione induction of sufAp acted independently. The rpoS mutation increased sufA induction by menadione, suggesting that the stationary sigma factor RpoS acts negatively on sufA induction.

Irradiation-resistant Properties of Structurally Controlled Molybdenum Alloys Through a Multi-step Internal Nitriding

  • Nakahara, Takayuki;Okamoto, Yoshihisa;Nagae, Masahiro;Yoshio, Tetsuo;Kurishita, Hiroaki;Takada, Jun;Hiraoka, Yutaka;Takida, Tomohiro
    • 한국분말야금학회:학술대회논문집
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    • 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part2
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    • pp.1161-1162
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    • 2006
  • In order to overcome the recrystallization embrittlement and irradiation embrittlement of Mo, which are major problems for its fusion applications, internally nitrided Mo alloys were prepared by a novel multi-step internal nitriding. Neutron irradiation was performed in the Japan Material Testing Reactor (JMTR). After irradiation, nitrided Mo alloys exhibited $\iota$ ower ductile-brittle transition temperature than irradiated TZM. These results suggested that multi-step internal nitriding was effective to the improvement in the embrittlement by irradiation. Transmission electron microscope observation revealed that TiN particles precipitated by nitriding acted as a sink for irradiation-induced defects.

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XPS STUDY ON THE EFFECT OF LOW-ENERGY ELECTRON IRRADIATION ON DNA DAMAGE BY Fe3+ ION

  • Noh, Hyung-Ah;Park, Yeun-Soo;Cho, Hyuck
    • Journal of Radiation Protection and Research
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    • 제40권2호
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    • pp.87-91
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    • 2015
  • We have employed X-ray photoelectron spectroscopy (XPS) technique to examine the combined effects of low-energy electron (LEE) irradiation and $Fe^{3+}$ ion on DNA damage. pBR322 plasmid DNA extracted from E. coli ER2420 was used for preparing DNA-$Fe^{3+}$ sample. The C1s XPS spectra were scanned for LEE-irradiated and LEE-unirradiated samples and then curve-fitted. For the samples with LEE irradiation only or with Fe ion only, no significant changes from pure DNA samples were observed - a single effect of either $Fe^{3+}$ ion or LEE irradiation did not cause a significant damage. However, when these two components were combined, the DNA damage was increased quite significantly, compared to the sum of DNA damages caused by $Fe^{3+}$ ion and by LEE irradiation independently. This observation is consistent with our previous results [Radiat. Res. 177, 775 (2012)] which was done using gel-electrophoresis technique. Partial interpretation of the observed spectrum peaks was also attempted.

가미지황탕(加味地黃湯), 가미사군자탕(加味四君子湯) 및 가미군자지황탕(加味君子地黃湯)의 방사선(放射線) 부작용(副作用) 감소효과(減少效果) (Study on Radioprotective Effects of Kamisagoonjatang, Kamijihwangtang and Kamigoonjajihwangtang)

  • 김동희;최봉균;조문준;김성훈
    • 혜화의학회지
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    • 제8권1호
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    • pp.115-129
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    • 1999
  • To evaluate radioprotective effects of Kamisagoonjatang(KST), Kamijihwangtang (KJT) and Kamigoonjajihwangtang(KKJT), studies were done experimentally. The results were obtained as follows: 1. By FACS analysis after exposure to radiation by Liniac, T cell and T-helper cell were significantly increased in KST treated group and also B cell and macrophage in KJT treated group while splenocytes were significantly decreased in control group. 2. WBC, PLT were significantly increased in KKJT treated group as compared with control group after exposure to radiation by Liniac. 3. In histological changes of jejunum of $BALB{\backslash}C$ mice after after exposure to radiation by Liniac, exclusion and fusion of villi were decreased in all groups as compared with control group. 4. In the observation of morphological changes by SEM and TEM after radiation by Liniac, KKJT, KJT and KST inhibited demage of internal structures such as mitochondria, ESR and golgi of jejunum cells in order as compared with control group. From above results it was concluded that KJT, KST and KKJT could be usefully applied for protection from damage by radiotherapy to cancer.

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