• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.161-167
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• 2015

Sweetpotato [Ipomoea batatas (L.) Lam] grows well in harsh environmental conditions, and is cultivated as one of the top seven food crops in the world. Recently, sweetpotato is drawing interest from people as a healthy food because it is high in dietary fiber, vitamins, carotenoids and overall nutrition value. However, few studies have been conducted on sweetpotato genome sequencing in spite of its importance. This review is aimed at increasing the efficiency of sweetpotato genome sequencing research as well as establishing a base for gene utilization in order to control useful traits. Recently, animal and plant genome sequencing projects increased significantly. However, sweetpotato genome sequencing has not been performed due to polyploidy and heterogeneity problems in its genome. Meanwhile research on its transcriptome has been conducted actively. Recently, a draft of the diploid sweetpotato genome was reported in 2015 by Japanese researchers. In addition, the Korea-China-Japan Trilateral Research Association of Sweetpotato (TRAS) has conducted research on gene map construction and genome sequencing of the hexaploid sweetpotato Xushu 18 since 2014. The Bill & Melinda Gates Foundation launched the 'sweetpotato genomic sequencing to develop genomic tools for Sub-Sahara Africa breeding program'. The chloroplast genome sequence acquired during sweetpotato genome sequencing is used in evolutionary analyses. In this review, the trend of research in the sweetpotato genome sequencing was analyzed. Research trend analysis like this will provide researchers working toward sweetpotato productivity and nutrient improvement with information on the status of sweetpotato genome research. This will contribute to solving world food, energy and environmental problems.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.327-336
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• 1995

This study was carried out to develop the animal protein(feed for fry) that was isolated, purified and lyophilized the rumen ciliates from the healthy rumen contents which have $10^5-10^6/g$ ciliates and were discarded in abattoirs. The rumen ciliates are non-pathogenic, anaerobic and the weight of this protozoa is 2% of rumen content. The rumen protozoan and bacterial proteins both have a biological value for rats of 80-81, which is higher than the 72 of brewer's yeasts. Furthermore, the true digestibility and net protein utility of the protozoan protein are 91 and 73, much higher than those of bacterial(74 and 60) or yeast(84 and 60) proteins. The amino acids of rumen protozoa is nutritionally superior than the others. The size of rumen ciliates is $30-200{\times}20-110{\mu}m$ and so we had isolated and purified the rumen ciliates from the rumen contents by the physical methods. The purified rumen protozoa was lyophilized with freezing dryer. The results of this experiment were as follows : 1. Population dynamics of protozoan ciliates in slaughtered rumens; % of samples which small ciliates were predominated was 82.5%(52/63) and that of large ciliates was 17.5%(11/63). 1) predominant species of small ciliates were Entodinium ovinum and E nanellum. 2) predominant species of large ciliates were Epidinium ecaudatum and Diploplastron affine. 2. The lyophilized rumen ciliates which were isolated and purified from 1 kg of rumen content at the pH 6.2-6.8 was about 7.0 gram. 3. The nutrient analysis of lyophilized rqmen ciliates(LRC) was as follows: 1) Proximate analysis of the LRC and the composition of fry feed; moisture 8.05%(below 10.0), protein 35.37%(45), fat 5.39%(4.5), fiber 1.23%(below 2.5), ash 2.25%(below 15.0), Ca 0.26%(below 2.0), P 0.14%(below 1.1), energy 4,608.11(fish meal 5000 cal/g) 2) Amino acids (% in crude protein) of the LRC and the rotifer(Brachionus plicatilis); Arg 5.19%(4.50), His 2.50%(1.55), Ile 5.29%(3.45), Leu 8.11%(5.85), Lys 10.34%(6.15), Met 2.25% (0.85), Phe 5.66%(3.80), Thr 5.14% (3.45), Val 4.18%(3.90), Ala 4.13%(3.35), Asp 13.26%(8.25), Glu 16.62%(9.20), Gly 4.23%(3.10), Pro 3.25%(5.05), Ser 4.85%(3.85), Tyr 5.04%(3.05) 3) Fatty acids(% in fat) of the LRC and the rotifer(biological feed ; Brachionus plicatilis); myristic acid(C14:0) 3.27%(0.3), myristoleic acid(C14:1) 0.83%(-), palmitic acid(C16:0) 39.11% (23.5), palmitoleic acid(C16:1) 2.81%(2.0), stearic acid(C18:0) 9.36%(5.6), oleic acid(C18:1) 25.54%(3.5), linoleic acid(C18:2) 15.05%(32.9), linolenic acid(C18:3) 1.74%(9.8). Judging from the above investigated results, the analytical data of proximate analysis, amino acids, fatty acids of the purified and lyophilized rumen protozoa are reasonable for the feed of freshwater fishes(fry and fingerling). But it was disappointed of our expectation that the crude protein of lyophilized rumen ciliates contains low percentage, it was thought that because of the small ciliates(starch digester) in beef cattle rumens which were administered the concentrated feed, is much difficult to isolate and purify than the large ciliates(fiber digester).

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.6
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• pp.1142-1149
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• 2002

This study was designed to standardize recipe and analyze the nutrients of Aguyjjim (stewed angler), a kind of native local foods in Busan. The results of this study are summarized as follows. The form of angler and its way to catch for a bait were recorded in Jasanobo, but the recipe was not written. While angler was used as manure or feed once, it was boomed for Aguyjjim since 80's, accredited as high-quality fish and protein-rich food. The standard recipe of Aguyjjim was prepared (through 3 times experimental cooking), being based on the recipe of cookbooks and restaurants. Then standard recipe was subjected to sensory evaluation. All sensory properties (appearance, smell, texture etc.) of Aguyiiim were evaluated as “like slight S, moderately”in hedonic scale. Energy per 100 g of the Aguyjjim was 89 kcal. It contained protein (10.7 g), fat (1.3 g), fiber (2.6 g), Ca (45 mg), Fe (1.3 mg) and so on. Amino acid is mainly comprised glutamic acid and aspartic acid. Free amino acid contents such as asparagine, glutamic acid, taurine were high. Fatty acid primarily consisted of unsaturated fatty acid like oleic acid, linoleic acid. Traditional foods are based on that of local foods. However, there has been little study looking into the recipes and nutritional value of local foods. Aiming at development of traditional food, the present study offered the standard cuisine of Aguyiiim that is a kind of local foods in Busan. In addition, it presented the composition of nutrients, amino acids and fatty acids. This data would be helpful for houses, restaurants and food service facilities to revive the local foods.

• Journal of the Korean Geographical Society
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• v.28 no.1
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• pp.1-15
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• 1993

This research aims to investigate some respects of chemical weathering processes, espcially the amount of solute leaching, formation of clay minerals, and the chemical weathering rate of granite rocks under present climatic conditions. For this purpose, I investigated geochemical mass balance in a small catchment and the mineralogical composition of weathered bedrocks including clay mineral assemblages at four res-pective sites along one slope. The geochemical mass blance for major elements of rock forming minerals was calculated from precipitation and streamwater data which are measured every week for one year. The study area is a climatically and litholo-gically homogeneous small catchment($3.62Km^2$)in Anyang-shi, Kyounggi-do, Korea. The be-drock of this area id Anyang Granite which is composed of coarse-giained, pink-colored miner-als. Main rock forming minerals are quartz, K-Feldspar, albite, and muscovite. One of the chracteristics of this granite rock is that its amount of Ca and Mg is much lower than other granite rock. The leaching pattern in the weathering profiles is in close reltion to the geochemical mass balance. Therefore the removal or accumulation of dissolved materials shows weathering patterns of granite in the Korean peninsula. Oversupplied ions into the drainage basin were $H^+$, $K^+$, Fe, and Mn, whereas $Na^2+$, $Mg^2+$, $Ca^2+$, Si, Al and $HCO-3^{-}$ were removed from the basin by the stream. The consumption of hydrogen ion in the catchment implies the hydrolysis of minerals. The surplus of $K^+$ reflects that vegetation is in the aggravation stage, and the nutrient cycle of the forest in study area did not reach a stable state. And it can be also presumed that the accumulation of $K^+$ in the top soil is related to the surplus of $K^+$. Oversupplied Fe and Mn were presumed to accumulate in soil by forming metallic oxide and hydroxide. In the opposite, the removal of $Na^+$, Si, Al resulted from the chemical weathering of albite and biotite, and the amount of removal of $Na^+$, Si, Al reflected the weathering rate of the bedrock. But $Ca^2+$ and $Mg^2+$ in stream water were contaminated by the scattered calcareous structures over the surface. Kaolinite is a stable clay mineral under the present environment by the thermodynamical analysis of the hydrogeochemical data and Tardy's Re value. But this result was quite different from the real assemblage of clay miner-als in soil and weathered bedrock. This differ-ence can be explained by the microenvironment in the weathering profile and the seasonal variation of climatic factors. There are different clay forming environments in the stydy area and these differences originate from the seasonal variation of climate, especially the flushing rate in the weathering profile. As it can be known from the results of the analysis of thermodynamic stability and characteristics of geochemical mas balance, the climate during winter and fall, when it is characterized by the low flushing rate and high solute influx, shows the environmental characteristics to from 2:1 clay minerals, such as illite, smectite, vermiculite and mixed layer clay minerals which are formed by neoformation or transformation from the primary or secondary minerals. During the summer and spring periods, kaoli-nite is a stable forming mineral. However it should consider that the other clay minerals can transformed into kaolinite or other clay minerals, because these periods have a high flushing rte and temperature. Materials which are directly regulated by chemical weathering in the weathered bedrock are $Na^+$, Si, and Al. The leaching of Al is, however, highly restricted and used to form a clay mineral, and that of Si falls under the same category. $Na^+$ is not taked up by growing veget ation, and fixed in the weathering profile by forming secondary minerals. Therefore the budget of $Na^+$ is a good indicator for the chemical weathering rate in the study area. The amount of chemical weathering of granite rocks was about 31.31g/$m^2+$/year based on $Na^+$ estimation.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.1
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• pp.35-46
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• 2009

In order to investigate spatial and temporal distributional characteristics of major water qualities in the Saemangeum area during the Saemangeum dike construction, salinity, COD, dissolved nutrients(DIN, Silicate) and heavy metals were analyzed from the surface water collected in April, May, August and November 2002. The overall value of Salinity, COD, DIN, and silicate in surface waters were in the range of $13.08{\sim}31.96\;psu$, $0.12{\sim}3.43\;mg/L$, $0.001{\sim}2.638\;mg/L$, and $0.010{\sim}3.181\;mg/L$, respectively. The COD and DIN in each survey showed the highest concentration at the mouth of Mangyeong river estuary(St. 1) where freshwater flow into the Saemangeum area. The concentrations of nutrients were high in the inner part of the Saemangeum dike with low-salinity, and low nutrients in the outer part of the dike with high-salinity, which strongly indicated that concentrations were adjusted by physical mixing. The ranges of dissolved metals and acid-soluble Hg in surface seawater were $0.006{\sim}0.115{\mu}g/L$ for Co, $0.26{\sim}0.114{\mu}g/L$ for Ni, $0.14{\sim}0.93{\mu}g/L$ for Cu, $0.04{\sim}0.53{\mu}g/L$ for Zn, $0.010{\sim}0.043{\mu}g/L$ for Cd, $0.010{\sim}0.795{\mu}g/L$ for Pb, and $0.25{\sim}4.16{\mu}g/L$ for Hg. The highest concentrations of some metals except for Cd were found at the estuary(Sts. 1 or 3). In most cases, a decreasing order of metal concentrations towards open sea(low-salinity$\rightarrow$high-salinity) was observed and showed positive relationship with DIN and silicate caused by land base pollutants input. On the other hand, due to Cd desorption from suspended solids in saline water, dissolved Cd concentrations were high in high-salinity area and low in low-salinity. In November, Co, Zn, Cu and Pb were relatively high in the northern area of the outer-side of Saemangeum, which was only influenced by the Geum river discharge. The concentrations of most dissolved metals of this study were lower than those of the past data in this area, but higher than those in Lena river estuary under the pristine environment.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.761-772
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• 2007

This study was conducted to evaluate effects of dietary pine cone meal on growth performance, blood characteristics, carcass quality and fecal noxious gases compounds in finishing pigs. The total of sixty [(Landrace×Yorkshire)×Duroc] pigs(86.01±0.25kg in average initial body weight) were used in 35 days assay. Dietary treatments included 1) T1(2% cottonwood sawdust), 2) T2(1% cottonwood sawdust+1% pine cone meal) and 3) T3(2% pine cone meal). There were three dietary treatments with five replicate pens per treatment and four pigs per pen. During the overall periods, there were no significant differences in ADG(Average daily gain), ADFI(Average daily feed intake) and gain/feed ratio among treatments (P>0.05). Also, Nutrient digestibilities and blood characteristics were not affected by dietary treatments. At the end of this experiment, a*-value of logissimus dorsi muscle color and sensory evaluation color were higher in T3 treatment than T1 treatment(P<0.05). In fatty acid contents of lean, C18:1 and total MUFA were significantly lower in T1 treatment than other treatments(P<0.05). However, total ω6 and total PUFA were higher in T1 treatment than T2 treatment(P<0.05). In fatty acid contents of fats, total SFA was significantly higher in T2 treatment than T3 treatment(P<0.05). C18:1 was higher in T2 treatment than T1 treatment(P<0.05). There were no significant differences in fecal noxious gas compounds among the treatments. In conclusion, the results of the experiment was affected by dietary supplementation of pine cone meal on meat color and fatty acid composition of pork in finishing pigs.

• Korean Journal of Poultry Science
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• v.34 no.1
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• pp.43-52
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• 2007

Experiments were conducted to investigate the effects of dietary botanicals (herbs and plant extracts) on the performance, nutrient metabolizability, small intestinal microflora, IgG level and blood parameters in broiler chickens. In Exp. 1, 1,000 (500 each sex) broiler chicks($Ross^{(R)}$) were divided into 20 groups of 50 chickens each(25 birds each sex). Four groups were assigned to each of five dietary treatments:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Herb M(Herb $mix^{(R)}$), Plant extract B(BIOSTRONG $510^{(R)}$) and Plant extract A($APEX^{(R)}$). In Exp. 2, 240(120 each sex) broiler chicks($Ross^{(R)}$) were devided into six treatment groups:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Plant extract D($Digestarom^{(R)}$), Plant extract P($Phellozyme^{(R)}$), Plant extract G($Galicin^{(R)}$) and Plant extract C(CRINA $POULTRY^{(R)}$). Each treatment consisted of four replicates of 10 birds each. In both experiments, birds had free access to diets and water for 5 wk on floor pens(Exp. 1) and cages(Exp. 2). In Exp.1, production index of groups fed diets supplemented with herbs and plant extracts was slightly higher than the control and those fed Herb M was highest. In Exp. 2, groups fed diets supplemented with herbs and plant extracts consumed more feed than the control during the period between 4 and 5 wk(P<0.05). Feed conversion(feed/gain) was lower in antibiotics group than other groups. The values of RBC, Hb and HCT were higher(P<0.05) in chicken fed diets supplemented with the additives than in the control in Exp. 1. BA value was lower(P<0.05) in groups fed diets supplemented with the additives than in the control in Exp. 2. Serum IgG were higher(P<0.05) in groups fed diets supplemented with the additives than in the control in both experiments. The cfu of intestinal microflora and metabolizability of nutrients were not significantly different among treatments in both experiments. It was concluded that the botanical supplements can be used as an alternative to antibiotics in broiler diets.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.706-713
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• 2013

With pot-grown 4-year-old 'Fuyu' persimmon trees, this study evaluated the effect of different nitrogen (N) rates during summer on fruit characteristics, changes of leaf nutrients after harvest, reserve accumulation, and early growth the following year. A total of 0, 36 g N in June, and 72 g N in June and July was fertigated to each tree using urea solution. All the fruits were harvested on Nov. 3. Although not significant, fruits were larger for the 36 g and 72 g N than the 0 g N. Fruits for the 0 g N, having lower N concentration, were softer and had a better coloration and higher soluble solids, indicating that they matured earlier. SPAD value on Nov. 3 was 19.2 for the 0 g N and 54.9 for the 72 g N, and then the values linearly decreased in all the treatments by Nov. 14, exhibiting rapid leaf senescence. Specific leaf weight, being the lowest for the 0 g N, also gradually decreased during this period. Increasing N level significantly increased cross-sectional area of the trunk. Leaf N concentration on Nov. 3 was 0.87% for the 0 g N, whereas it was 1.18 and 1.52% for the 36 g and 72 g N, respectively. The N fertigation tended to increase leaf concentrations of soluble sugars, starch, and amino acids. Contents of N, P, K, soluble sugars, starch, and amino acids per unit leaf area gradually decreased in all the treatments during the 11 days after harvest, and the extent of the decrease was the lowest for the 0 g N. On the other hand, those of Ca, Mg, and protein did not consistently change during this period. The N fertigation resulted in higher concentrations of N in dormant shoots on Nov. 14, and although not great, it also increased soluble sugars, starch, amino acids, and protein. Clear differences were found in number of flower buds per one-year-old branch and total shoot length per tree the following year. The 72 g N trees had 5.6-fold more flower buds and 1.9-fold more shoot length, compared with those of 0 g N trees. However, it was noted that tree growth the following year was not significantly different between the 36 g and 72 g N the previous year. It was concluded that N rate during summer should be adjusted with considering the changes of fruit maturation, mobilization of leaf nutrients, and reserve accumulation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.245-250
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• 2005

This experiment was conducted to compare the frech and dry matter yields and feed values of Sorghum$\times$Sudangrsss Hybrid in accordance with different nitrogen and potassium fertilizer sources at the Dae-Ho reclaimed tidal land, Korea from 2003 to 2004. Soil salt contents of ammonium sulfate plots(T3, T4, T5) were higher than that of the urea plot(T2), but that of potassium sulfate plot(T6) was the lowest. The fresh yields of ammonium sulfate plots(T3, T4, T5) and potassium sulfate plot(T6) were higher than that of the urea plot(T2) as $173\%,\;173\%,\;144\%\;and\;90\%$. respectively. The dry matter and total digestible nutrient(TBN) yields were similar tendency like the results of the fresh matter yields. The crude protein(CP), neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of ammonium sulfate plots(T3, T4, T5) were higher than those of urea plot(T2), but those of potassium sulfate plot(T6) were the lowest. On the other hand, TDN content in potassium sulfate plot(T6) was the highest. It was desirable to use ammonium sulfate$(20\~30kg\;N/10a)$ and potassium sulfate fertilizer$(15kg\;K_2O/10a)$ than those of urea and potassium chloride fertilizer on reclaimed tidal land in view of forage production and its feed value.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.207-216
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• 2004

This study was carried out to select the promising forage crops for forage production in paddy field of NLRI, RDA, Suwon from 1999 to 2001. The species of forage crop used in this experiment were barley(3 varieties), wheat(2), rye(3) and Italian ryegrass(IRG, 3). Stages of heading, milk and yellow ripe of barley were 1 May, middle May and late May, respectively. In milk to yellow ripe stage, dry matter(DM) and total digestible nutrient(TDN) yield of barley were $7.30{\sim}9.58$ MT and $4.75{\sim}6.24$ MT per ha, and Albori was the most promising variety among the barley. The growth of wheat was a little late compared to that of barley. but it seemed to be crop having higher forage yield and nutritive value. In milk to yellow stage, DM and TDN yield of wheat were $8.17{\sim}10.82$ MT and $5.33{\sim}7.31$ MT per ha. Heading stage of rye was 27 to 29 April, and it seemed to be suitable for harvest at heading to flowering stage because of heavy lodging after flowering. In flowering stage, DM and TDN yield of rye were 10.18 and 6.03 MT per ha. Heading stage of early maturing IRG was 7 May and it seemed to be good for harvest at flowering stage of middle May in cropping system. In flowering stage, DM and TDN yield of early type IRG were 4.48 and 2.96 MT per ha. The results demonstrated that the promising forage crops for forage production in paddy. field were rye and barley(Albori) in Middle region, and rye harvesting at early May was recommended when considered middle May of rice transplanting period, and rye and barley considered late May of rice planting period. Also early maturing and high yielding IRG variety was strongly needed.