• Title/Summary/Keyword: nut

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Comparison of methods of DNA extraction from tree nuts (견과류로부터 효율적인 DNA 추출 방법 비교)

  • Suh, Seung-Man;Park, Saet-Byul;Kim, Mi-Ju;Kim, Hae-Yeong
    • Korean Journal of Food Science and Technology
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    • v.50 no.4
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    • pp.357-361
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    • 2018
  • This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.

Leakage-reduction Measures at a Joint between CPVC Piping for a Sprinkler System and a Pipe Expansion (스프링클러설비용 CPVC관과 신축배관 접속부분에서의 누수저감 대책에 관한 연구)

  • Lim, Chun-Ki;Lim, Yun-Tack;Baek, Eun-Sun
    • Fire Science and Engineering
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    • v.29 no.3
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    • pp.21-30
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    • 2015
  • In this study, we try to suggest measures to reduce leakage at a joint between CPVC piping for a sprinkler system and a pipe expansion through reviews of domestic and foreign standards and related tests. The quality of the waterproof rubber packing material between a valve socket and pipe expansion nut was examined. In the leak test, the valve socket material over the expansion part of the metal pipe nut was found to use a metal part or a schlorinated polyvinyl chloride pipe nut part. In addition, the KS B 0221 standard for parallel pipe threads with threaded and thread inspection criteria and inspection standards in order to ensure an acceptable quality of valve socket, there is a need to amend the regulations to comply with the KS B 5223 (screw thread limit gauges parallel pipe threads). We do not have detailed standards for expansion piping nuts for waterproof rubber ring material, so we need to amend the relevant criteria for EPDM material to be used with excellent waterproofing, for which both NBR and EPDM are currently used.

Evaluation of Pull-Out Strength of Connections with Roof Cladding using Honey Comb Panel Secured Cool Roof Performance (Cool Roof 성능을 확보한 Honey Comb Panel 지붕 접합부의 인발 성능 평가)

  • Lee, In Ho;Park, Sang Woo;Ko, Kwang Il;Chung, Mi Ja;Lee, Eun Taik
    • Journal of Korean Society of Steel Construction
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    • v.28 no.3
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    • pp.139-149
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    • 2016
  • Roof cladding of buildings are required for the measures about the 'screw pull-out' which causes the casualties and the property damage by typhoons. In this study, the pull-out resistance was increased by increasing the penetration depth of the screw installing a ironware called 'insert nut' on the roof cladding frame. Tensile tests were conducted to compare the pull-out strengths of a general screw-joint and a nut insert joint. Roof cladding that is actually being used in the field was produced using the 'solid work' and then the roof claddings using a general screw-joint and a nut insert joint were compared by a static test and dynamic test.

The Joining Quality of High Strength Bolt, Nut and Washer Set (A490) in the Extreme Conditions (극한 상태에서 A490 고장력 볼트 세트의 체결 품질 연구)

  • Suk, Han-Gil;Cho, In-Seup;Hong, Hyeon-Sun
    • Journal of the Korean institute of surface engineering
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    • v.44 no.1
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    • pp.21-25
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    • 2011
  • This test focuses on the correlation between the lubrication, ductility and strength through the change of nut lubricant which decides joining angle for A490 bolt sets required in the AASHTO (American Association of State Highway & Transportation Officials). Because the lubrication of high tension bolt, nut, washer sets becomes an important factor to ductility and joining load, the quality improvement is required for improving reliability and securing enough lubrication of maker and user. This study examines the quality characteristics required in the specification through tests because only this standard specifies joining quality in the extreme conditions (as much about two times of the joining angle on site as normal condition). Moreover, this study is limited to the test on joining axial force required in the AASHTO for the three nut lubrication conditions of A490 bolt set. It is concluded that the nuts should be coated or waxed enough for the improvement of the joining axial force and ductility of bolts required in the standards. It is shown that in the case of plain high tension bolt sets, a rust preventing lubricative oil shall be applied and the torque coefficient value for A490 bolt sets should be maintained below 0.175.

An Evaluation of Clamping Characteristics for High Strength Bolts with Variable Bolt Lengths (고력볼트 길이에 따른 체결 특성 평가)

  • Lee, Kang Min;Kim, Kang Seok;Nah, Hwan Seon;Lee, Hyeon Ju;Oh, Kyung Hwan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.15 no.6
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    • pp.127-134
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    • 2011
  • It has been reported that the length parameter of high strength bolts results in the variance in tensile loads. The required turn of nut for each length is specified in AISC RCSC specification. There is no specific regulation datum about the bolt length in the two national codes and specifications in Korea. Therefore this study focused on evaluating influence of the clamping torque subjected to length parameter of high strength bolts. Two types of high strength bolt specimens were manufactured and tested; High Strength Hexagon bolt specified in ASTM A490 and Torque Shear Bolt in KS B 2819. The length parameter ranged from 60mm(3d) to 140mm(7d). The torque, turn of nut, and the clamping force were analyzed to review whether length parameter affects on the required tensile strength. To evaluate the effects of the length parameter on the torque and turn of nut for the required strength and clamping force, statistical analysis was also carried out.

Gallotannins from Nut Shell Extractives of Camellia oleifera

  • HE, Yi-Chang;WU, Mei-Jie;LEI, Xiao-Lin;YANG, Jie-Fang;GAO, Wei;BAE, Young-Soo;KIM, Tae-Hee;CHOI, Sun-Eun;LI, Bao-Tong
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.3
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    • pp.267-273
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    • 2021
  • Camellia nut shell was collected, dried at room temperature and ground to get fine powder. The powder was extracted three times with 95% EtOH, combined, evaporated, and then freeze dried. The crude powder was dissolved in H2O and then sequentially fractionated with n-hexane, CH2Cl2, EtOAc and n-BuOH. A part of EtOAc fraction was chromatographed on a silica gel and on a Sephadex LH-20 columns using MeOH, aqueous MeOH, EtOAc-n-hexane and EtOH-n-hexane to isolate gallotannins. Three gallotannins, 1,2-di-O-galloyl-β-D-glucopyranoside (2), 1,2,6-tri-O-galloyl-β-D-glucopyranoside (3) and 1,2,3,6-tetra-O-galloyl-β-D-glucopyranoside (4), including gallic acid (1), were isolated and elucidated by NMR and Mass spectroscopies. Although nothing new, these gallotannins were first reported from the nut shell extractives of camellia tree (Camellia oleifera C. Abel). This study was to investigate the chemical constituents, especially hydrolysable tannins, of nut shell extractives of Camellia oleifera and to provide basic information for the future chemical utilization of this species.

Effect of Clearance on Clamping Characteristics of Segmented Nuts in Pyrotechnic Separation Nut (파이로테크닉 분리 너트 내부 분할 너트 체결 특성에 대한 틈새의 영향)

  • Woo, Jeongmin;Kang, Dahoon;Choi, Jaeyoung;Kim, Jeong Ho;Cho, Jin Yeon;Jang, Seung-gyo;Yang, Hee Won
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.12
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    • pp.994-1003
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    • 2018
  • The clamping characteristics of segmented nuts in pyrotechnic separation nut are investigated using finite element analysis. When the segmented nuts are used to clamp objects, a constraint cylinder is necessary to prevent the segmented nuts from spreading out to the radial direction. During the manufacturing process, a clearance is usually introduced between the outer diameter of the segmented nuts and the inner diameter of the constraint cylinder. Therefore to find out the effect of the clearance, proper finite element modeling method is suggested to describe the clamping procedure appropriately. In addition, finite element analysis with the clearance of 0.00, 0.03, 0.06, and 0.10 mm are performed. From the analysis results, the clamping characteristics of segmented nuts are investigated according to the clearance, and several factors which should be considered to design a reliable pyrotechnic separation nut are figured out.

A study on the development of dimension inspect Program for the vehicle axle casing nut welding part using digital image processing (디지털 화상처리를 이용한 차축 Casing Nut 용접부 치수 검사 프로그램 개발에 관한 연구)

  • 김재열
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.9 no.5
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    • pp.135-143
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    • 2000
  • The vision system is easy to use the exclusive use as a independent computer system but it is not much popularization by reason of expensive and difficult to adapt to the various fields, because it is easy to the existed computer system, the price is cheap and also it can use to the various measurement purpose which user wanted and programed. The measurement method of the vehicle axle casing nut welding part is that measure the value of the welding part to adapt the actual operation program from using the ratio between the actual length of the standard specimen and the length of image, to measure the ratio between the actual product and the camera image. A defect is found by the assembled visual inspection system. The inspection algorithm which estimates the quality of welded product is developed and also, the software program which processes the automatic test function of the inspection system. We make the foundation of the inspection automatic system and we will help to apply other welding machine.

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A Study on Horticulture Foods in Kory$\v{o}$ Era (고려시대의 원예식품류에 관한 연구)

  • 강춘기
    • The Korean Journal of Food And Nutrition
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    • v.3 no.1
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    • pp.69-79
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    • 1990
  • Koryo(918-1352) made a certain progress in her culture and agriculture except the latter part of the era when the development in social economy were retarded due to military dictatorship and Mongol invasion. Despite of these external conditions, cultivations and kinds of horticulture foods were expanded With the help Of many king's agriculture first policy and the advancement in cultivating method . Among the horticulture foods, fruits such as peach, plum, Japanese apricot, apricot, cherry, pear, persimmon, pomegranate, crab apple, Jujube, grape, Chinese quince, walnut, orange, yuzu, chestnut, ginkgo nut(silvernut), pine nut, nutmeg nut, and fiat were crowed, and vegetables such as Chinese cabbage, turnip, radish, garlic, welsh onion, gynmigit, scallion, taro, malva, cucumber, white gourd, bottle gourd, water melon, eggplant, Japanese ginger, ginger and litchi were cultivated, while semi, water shieled and bamboo sprouts were taken in natural. Fruits were taken in natural or through dry, and particularly grapes were used to make wine, Flowers of Japanese apricots, some fruit trees, and chrysanthemum were also made into wine. Certain fruits were used as medicine owing to its medicinal nature. Vegetables were used to make Kimchi or to boil soup, sometimes they were dried to be kept in storage to be used in rare season and also used as medicine Increase in kinds of horticulture foods does not have any direct relation with the reform of social economy, but the fact that so many kinds of horticulture foods were cultivated and used in Koryo era shows that they elevated people's life and dietary culture.

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The Effects of Perilla and Pine Nut on Blood Components in Rats (들깨와 잣이 흰쥐의 혈액 성분에 미치는 영향)

  • Jho, Hu Jhong
    • Journal of Environmental Health Sciences
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    • v.10 no.1
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    • pp.99-106
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    • 1984
  • The diets used to this experiment were composed only of stock diet for group A, a mixture of stock diet and 2% cholesterol for group B, and 5%, 10% perilia powder for group C, D and 5%, 10% pine nut for group E, F in addition to group B diet respectively. All groups fed with these experimental diets for 3 weeks were fasted for 15 hours at the end of the experiment, and then they were decapitated to take serum. The serum was used to measure the level of glucose, total cholesterol, triglyceride, HDL-cholesterol and lipoprotein composition. The results were summarized as follows. 1. Perilla and pine nut diets have no influence on body weight and food intake in cholesterolfed male rats 2. The blood glucose level was not changed significantly at groups C-F. 3. Total cholesterol level was significantly decreased by 21.6% ~ 29.7% at group C-F than at group B. 4. HDL-cholesterol level was increased by 51.3% ~ 86.1% at group C-F than at Group B. 5. The $\alpha/\beta$ ratio which is 0.51 at the group A was risen to 0.99 at the group B. However, this ratio was decreased to 0.5 0 - 0.51 at group C, D and to 0.68 - 0.69 at group E.F.

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