• 제목/요약/키워드: nursery media

검색결과 44건 처리시간 0.02초

Effects of Dietary Zinc on Performance and Immune Response of Growing Pigs Inoculated with Porcine Reproductive and Respiratory Syndrome Virus and Mycoplasma hyopneumoniae

  • Roberts, E.S.;Heugten, E. van;Spears, J.W.;Routh, P.A.;Lloyd, K.L.;Almond, G.W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권10호
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    • pp.1438-1445
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    • 2004
  • The objective of this study was to determine the effects of dietary Zn level on performance, serum Zn concentrations, alkaline phosphatase activity (ALP), and immune response of pigs inoculated with Porcine Reproductive and Respiratory Syndrome virus (PRRSv) and Mycoplasma hyopneumoniae. A $2{\times}4$ factorial arrangement of treatments was used in a randomized design. Factors included; 1) PRRSv and M. hyopneumoniae inoculation (n=36 pigs) or sham inoculation (n=36 pigs) with media when pigs entered the grower facility (d 0) at 9 weeks of age and 2) 10, 50, 150 ppm supplemental Zn sulfate (${ZnSO}_4$) from weaning until the completion of the study, or 2,000 ppm supplemental ${ZnSO}_4$for two weeks in the nursery and then supplementation with 150 ppm ${ZnSO}_4$for the remainder of the trial. The basal diet contained 34 ppm Zn. Pigs were weighed on d 0, 10, 17, 24 and 31 and blood samples were collected on d 0, 7, 14, 21 and 28. Pigs inoculated with PRRSv were serologically positive at d 28 and control pigs remained negative to PRRSv. In contrast, the M hyopneumoniae inoculation was inconsistent with 33.3% and 52.8% of pigs serologically positive at d 28 in the control and infected groups, respectively. A febrile response was observed for approximately one week after inoculation with PRRSv. Feed intake (p<0.01) and gain (p<0.1) were less in PRRSv infected pigs than control pigs for the 31 d study. However, performance did not differ among pigs in the four levels of ${ZnSO}_4$. Assessments of immune responses failed to provide unequivocal influence of either PRRSv inoculation or ${ZnSO}_4$level. These data suggest that PRRSv and M. hyopneumoniae act to produce some performance deficits and the influence of Zn supplementation of nursery age pigs does not have clear effect in grower pigs affected with disease.

가정학 전공자의 취업과 전망 (A Study of Current Employment and Future Trends for Young Home Economists)

  • 문수재
    • 대한가정학회지
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    • 제20권3호
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    • pp.85-102
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    • 1982
  • The current employment status among young home economists and perspectives in occupations for prospective Home Economics graduates were explored in this study which utilized information from 17 to 21 colleges in Korea and colleges in the United States during the five years of 1977∼1981. The Home Economics content areas covered in this study were Clothing and Textiles, Foods and Nutrition, Housing and Interior Design, and Child Development and Family Life. The highest percentage of Korean graduates in Clothing and Textiles was employed either as teachers at the junior high school level or as designers in the clothing and textile industries. Quite a number of the graduates were engaged in further studies at the graduate level. Korean graduates with a master's degree were teaching at the college level and some had furthered their studies at the doctorate level either here or abroad. Koreans with a bachelor's degree in Foods and Nutrition held jobs as teachers in junior high school, dieticians at mass feeding institutions and hospitals, food scientists in food industries, and researchers in institutions. Those with a master's degree were teaching at the college level. Americans with a bachelor's degree worked as dieticians, supervisors in restaurants and institutions, extension workers, researchers at various facilities, teachers and clerks. Americans with a master's or doctorate degree were engaged in teaching at colleges or supervising at research or working as extension specialists. In general, Korean graduates were found to hold positions in less varied areas than their American counter-parts. Among forty-nine graduates those working in their professional field reported less sex discrimination that those working in other fields. The major area of employment in Housing and Interior Design or Home Management graduates in Korea was teaching while in the United States it was extension work, business, governmental work and teaching. It was suggested that in the future, career development in Korea be further explored to include extension service, research, social welfare, financial planning, business, free-lancing, funeral home, home-call, and correctional education. Interviews with executives from 6 business enterprises indicated that most of them were aware of the potential contribution home economists could make for their companies but they expressed a negative attitude towards women in general due to their short stay on the job. Jobs held by Child Development and Family Life majors with a bachelor's degree in Korea were mostly teaching positions in public, junior and senior high school. However, jobs such as nursery school teaching, working in clinical setting, business, and teaching at public, junior and senior high school predominated in the United states. Most Korean graduates with a master's degree were teaching in professional colleges while in the United Stated the job variation among the graduates was rather evenly distributed among teaching at college level, public and high school, nursery school and administration areas. Reports from 7 child development majors on the job indicated that they were paid less that secretarial workers. Only half of them were working in their major area and these expressed satisfaction with their work. Two thirds of the respondents indicated no sex discrimination. It was suggested that in the future Child Development and Family Life majors pursue employment in counseling, guidance, recreation, mass media, administration and outreach work as well as education, research and parent education in services for children, teen-agers, adults and families.

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상토에 함유된 Na함량이 오이와 참외의 생육, 광합성 및 잎의 수분상태에 미치는 영향 (Effect of Sodium in Artificial substrate on the Growth, Gas Exchange and Leaf Water Status of Cucumber (Cucumis sativa L.) and Korea Melon(Cucumis melo L.))

  • 서영진;김종수;김찬용;박소득;박만
    • 한국토양비료학회지
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    • 제41권3호
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    • pp.177-183
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    • 2008
  • 상토에 함유된 Na이온이 과채류의 육묘에 미치는 영향을 조사하기 위하여 온도조건에 따른 상토의 수분변화, Na이온 및 EC의 경시적 변화, 광합성율, 증산율, 잎의 수분상태, 묘소질, 생육 및 수량성에 대한 실험을 실시하였다. 육묘온도가 $15^{\circ}C$에서는 상토의 수분감소는 적어 3일이 경과하더라도 관수개시점에 도달하지 않았다. 관수 1시간 후 부터 상토로부터 Na 이온이 유리되어 22시간이 지날 때 까지 Na의 농도가 계속 증가하였으며 Na 함량의 증가에 따라 EC의 상승이 있었다. 또한 관수회수가 증가함에 따라 Na의 세탈에 따른 Na 농도가 감소되었고 Na 농도감소에 비해 EC의 감소폭은 적은 편이었다. 양이온 비율중 Na 함량이 60% 이상일 경우 저온에서는 광합성율, 증산율의 감소가 큰 편이었고 포화수분부족도 또한 저온에서 큰 폭으로 높아졌다. Na 함량이 높은 상토에서 육묘된 오이묘는 초장, 세근수 등이 대조상토에 비해 낮은 편이었고 본포 정식후에도 생육이 불량하여 48%정도의 수량감소를 초래하였다.

Differential Expression of a Chimeric nos-npt II Gene in 9 Years Old Hybrid Poplars (Populus koreana x P. nigra)

  • Noh, Eun Woon;Lee, Jae Soon;Choi, Young Im;Lee, Hyo Shin;Bae, Eun Kyung;Lee, Ji Hee
    • Journal of Plant Biotechnology
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    • 제6권1호
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    • pp.15-19
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    • 2004
  • The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

Effects of Organic Manure on Seedling Growth and Nodulation Capabilities of Five Popular Leguminous Agroforestry Tree Components of Bangladesh

  • Uddin, Mohammad Belal;Mukul, Sharif Ahmed;Hossain, Mohammed Kamal
    • Journal of Forest and Environmental Science
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    • 제28권4호
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    • pp.212-219
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    • 2012
  • An experiment was set to understand the seedlings growth and nodulation capabilities of five potentially important leguminous agroforestry tree species of Bangladesh in response to application of organic fertilizer. Study was carried out in nursery bed on the seedlings of Acacia mangium Willd., Acacia hybrid, Acacia auriculiformis A. Cunn. ex Benth, Albizia lebbeck (L.) Benth. and Leucaena leucocephala (Lam.) de Wit. after sowing their seeds in polybags filled with different medium. Farmyard manure or decomposed cow dung was applied as the source of organic fertilizer with other potting media. The growth and nodulation performance of seedlings of the selected species were measured three moths after the first seed was emerged. The different soil: organic manure ratios' used during the experiment were, 1:1, 2:1 and 3:1. The effects of organic fertilizer on growth and nodulation were compared to that of seedlings grown in control. The results revealed a positive correlation between seedling growth and the different doses of organic fertilization (i.e. seedling growth was enhanced significantly with different treatments containing higher proportion of organic fertilizer). In few cases a negative effect was observed after applying higher levels of organic manure. It was also apparent that the nodulation in terms of nodule number and size was inhibited significantly by the application of organic fertilizer.

상토의 물리.화학성이 시설하우스 묘삼의 생육에 미치는 영향 (Influence of Various Substrates on the Growth and Yield of Organically Grown Ginseng Seedlings in the Shaded Plastic House)

  • 최재을;이누리;한진수;김정선;조서리;심창용;최종명
    • 한국약용작물학회지
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    • 제19권6호
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    • pp.441-445
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    • 2011
  • This research was conducted to investigate the influence of variouis organic substrates on growth and yield of organically grown ginseng seedlings in a shaded plastic house. In the investigation of optimal substrate, the eight substrate were formulated by adjusting blending rate of peatmoss, perlite, coir dust(coco peat), and vermiculite. Then, the changes in physico chemical properties of root substrates as well as their influences on the growth characteristics and yield were determined at six months after sowing. The elevation of the blending rate of peatmoss from 50% to 70% with decrease in the rate of inorganic component (mixture of perlite and vermiculite) from 50 to 30% resulted in the increase in container capacities and decrease in total porosities and air-filled capacities. The concentrations of $NH_4-N$, $P_2O_5$ and K increased as the incorporation rate of castor seed meal, phosphate ore, and langbenite, respectively, were elevated during the root medium formulations. The PPV-1 and PPV-4 substrates produced high stem length, stem diameter, shoot fresh weight, leaf area and root length among eight substrate. Root fresh weight was heaviest in PPV-4 compound nursery media. The results of this experiment will be utilized in the new substrate application for ginseng organic culture in shaded vinyl house.

Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

  • Ali, Jafari mofidabadi;Mansooreh, Kamandloo;Hamid, Selamti
    • Journal of Forest and Environmental Science
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    • 제33권3호
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    • pp.197-201
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    • 2017
  • Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

줄기 절간조직 배양에 의한 교잡종 사시나무의 대량증식 (Multiple Shoot Induction from Ex Vitro and In Vitro Derived Stein Node Culture of Populus alba L.$\times$P.grandidentata Michx.)

  • Sung Ho SON;Richard B. HALL
    • 식물조직배양학회지
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    • 제22권3호
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    • pp.131-135
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    • 1995
  • 잡종 사기나무의 기내 대량증식을 목적으로 줄기절간 유래의 기내 및 기외시료를 이용하여 다경줄기 유도에 관여하는 식물생장 조절물질의 효과에 대하여 조사하였다. 기외시료의 측아를 제거한 다음 20에서 30$\mu$M 의 zeatin이 함유된 WPM 배지에 6주간 배양하였을때 각각 11개와 13개의 다경줄기가 유도되었다. 그러나 기외시료의 측아가 붙어있는 상태에서 기내배양하여 2주후에 측아를 제거하고 동일 배지에 배양하였을 경우에는 BA가 1.0에서 2.0 $\mu$M 함유된 배지에서도 13개와 15개의 다경줄기를 유도할 수 있었으며, 특히 이때 생산된 줄기의 상태는 아주 건전한 것으로 나타났다. 기내줄기를 사용하였을때는 20 $\mu$M의 zeatin을 처리하였을때 가장 많은 줄기를 생산할 수 있었으며, 생산된 조직배양묘는 일정기간 순화를 시킨 후 온실이나 포지에 식재가 가능하였다.

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농작업시 발생하는 화학적 및 생물학적 위험요인에 대한 유사노출작업군 설정 연구 (A Study on Establishment of Similar Expousre Groups(SEGs) for Chemical and Biological Risk Factors in Farm Work)

  • 이민지;신소정;김효철;허진영;안민지;김경란;김경수
    • 한국산업보건학회지
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    • 제30권3호
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    • pp.292-298
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    • 2020
  • Objectives: The aim of this research is to establish Similar Exposure Groups (SEGs) for chemical and biological risk factors that occur in farm work involving 24 tasks among 15 crops. Methods: To categorize SEGs, work type, work environment, and similar tasks for each crop were considered. After confirming the chemical risk factors (pesticides, inorganic dust-total dust and PM10, ammonia, and hydrogen sulfide) and biological factors (organic dust-total dust and PM10, and endotoxins) that occur in the crops and tasks, similar crops and tasks were selected as SEGs. Results: Among chemical risk factors, pesticides was selected for the SEGs, which was categorized by open field, greenhouse, fruit, and specialty crops. For inorganic dust, open field (plowing harrowing, seedling, planting, harvest, and sorting and packing) and specialty crops (plowing harrowing, seedling, planting, and harvest) were selected as SEGs. For ammonia and hydrogen sulfide, livestock (preparation of farm, management of nursery bed, feeding, shipment and manure treatment) were selected as SEGs. For biological risk factors such as organic dust (total dust, PM10) and endotoxins, open field (manure application), greenhouse (plowing harrowing, planting, manure application, and harvest), fruit (manure application), specialty crops (manure application, making furrows, mixing mushroom media, harvest, and sorting and packing), and livestock (preparation of farm, maintaining poultry litter, feeding, shipment and manure treatment) were selected as SEGs. Conclusions: To establish similar exposure groups in agriculture, it is important that the characteristics of each hazard factor are categorized by identifying risk factors occurring by tasks.

수경재배에서 토마토풋마름병의 전염경로 (Infection Route of Bacterial Wilt of Tomato Caused by Ralstonia. solanacearum in Hydroponic Culture)

  • 남기웅;문병우;김영호;이창희
    • 생물환경조절학회지
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    • 제18권2호
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    • pp.171-176
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    • 2009
  • 본 연구는 토마토 수경재배에서 풋마름 병원균의 분포와 침입 및 전파경로를 구명하여 풋마름병 방제의 기초 자료를 얻고자 수행하였다. 배양액 재배시스템에서 토마토 풋미름병의 발생정도별로 배양액탱크, 배지, 폐액에서 병원균의 밀도를 검정한 결과 20% 정도 발병된 포장의 폐액에서는 19,000cfu/mL의 밀도로 검출되었으며 연작연수가 많을수록 병 발생이 심하였다. 토마토 펄라이트 수경재배시스템에서 토마토 풋마름병의 발생전파 과정은 최초 발생지점으로부터 좌우로 급속히 전파되었다. 토마토 풋마름병 발생포쟁에서 병원균의 유입경로를 추적한 결과 육묘 중에 감염되는 경우와 웹스 주변의 이병된 토양에서 감염되는 경우로 크게 두 가지 방법으로 유입되는 것으로 생각된다. 또한 시판용 토마토 종자에서는 풋마름병원균이 검출되지 않았다.