• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.980-987
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• 2003

In this report, we investigated the detoxication effects of Saururus chinenis, Geranium nepalense, Lonicera japonica, Cassia obtusifolia, Glycyrrhiza uralensis, or their mixtures by employing acute toxicity tests for nicotine and dioxin. When fatal doses $(LD_{100}\;=\;42\;mg/kg)$ of nicotine were injected into the abdominal cavities of ICR mice, those treated with OHEM showed delayed paralysis, half the duration of hyperactivity, and a 73 % survival rate. The results revealed the strong detoxicating effects of the mixtures. We also measured the amount of the degradation product of nicotine and cotinine in humans. Consumption of OHEM promoted (he more specific) the metabolic pathways of nicotine, increasing continine excretion by 1.5 times. As a result the amount of cotinine in urine was reduced to less than 5% after treatment with OHEM. In order to test the toxicity of dioxin, we used TcnN(SD)BR rats exposed to TCDD. While TCDD treatment reduced the blood levels of hemoglobin and platelet, OHEM consumption relieved these effects and, furthermore, helped to recover the number of platelet to the normal level (p<0.05). Moreover, neutrophils (%) and monocytes (%), which were reduced by the injection of TCDD, recovered to normal levels upon treatment with OHEM. The amount albumin reduced by TCDD (p<0.05) normalized, while the activities of GOT and GTP increased by TCDD were reduced. Increases in total cholesterol and neutral fatty acids induced by TCDD were also reduced by OHEM injection (p<0.05). In the kidney, TCDD-induced rises in creatinine were suppressed by OHEM treatment, while decreases in iron levels from TCDD were raised to normal. The treatment of TCDD had more toxic effects in the blood and pancreas than on the liver, kidney and heart. On the other hand, the detoxication of OHEM had significant effects on the liver and pancreas. The normalization by OHEM of various clinical abnormalities induced by TCDD demonstrates the detoxicating effect of OHEM that ameliorates systemic metabolism not properly functioning.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.3
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• pp.294-298
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• 2009

A glasshous experiment was carried out in 2002 to evaluate growth differences between two wick types, and to determine the optimum number of wicks in a wick-based hydroponics for production of 'Dejima' seed potatoes (Solanum tuberosum L.). The six minitubers ($7.0{\pm}0.2g$/tuber) produced through aeroponics were planted in a polystyrene box (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing growth medium (perlite+peatmoss 1:2, v/v mixtures). Before the boxes were filled with the medium, 6 wicks per box were vertically and 2 to 10 wicks were horizontally installed, respectively, at the bottom of the boxes through holes. Comparing with 6 vertical wick treatment, 6 horizontal wick treatment increased the number of tubers per plant, but decreased average tuber weight. Yield of tubers greater than 5 g (seed potatoes) was not significantly affected by wick type. As the number of the horizontal wicks per box was increased from 2 to 6, the number of seed potatoes increased from 2.8 to 8.0/plant and then leveled off. Average tuber weight increased with increasing the number of the wicks up to 8 and then leveled off. As the number of the wicks per box was increased from 2 to 6, seed potato yield increased from 1.81 to 6.59 kg/$m^2$ and then slowly increased up to 10 wicks per box, reaching 12.9 kg/$m^2$. The results indicate that installing 8 wicks per box ($0.16m^2$) horizontally for the wick culture system in production of 'Dejima' seed potatoes is beneficial considering seed potato yield, cost of wicks, and convenient filling with medium.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.3
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• pp.11-19
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• 1988

The purpose of this study is to investigate ways to control the wind dispersal of contton-wrapped seeds of such poplars and willows as Poplus alba, P. tomentiglandulosa, P. euramericana, P. deltoides, and Salix pseudo-lasiogyne. These trees are hated by many people because of their seeds blowing all over the place during May. These cottony seeds can be nuisance to various types of outdoor activities, pose safely threat to drivers, become fire hazards during prolonged spring dry spells, and cause many types of health hazards of allergy such as sneezing. rhinitis, asthma, and skin rashes. Four control methods can be used to resolve the problem. First, pruning can be a solution, but it is unsatisfactory in terms of costs and outcome. Second, planting of male trees only can be a solution, but it is hard to identify sexes of saplings. Third, female trees can he replaced with other species. But it requires high costs and takes at least ten years to functionally replace the removed ones. As an alternative to such unsatisfactory control methods, the possibility of applying plant growth regulators has been investigated since 1983. During the pre-test, various concentrations and mixtures of them were either sprayed or injected, but failed to achieve any promising results. But the injection of a mixture made up of 0.75g of 2-chloroethane phosphonic acid with 0.2 mg of GA in 300cc water in the end of March produced premature falling of almost all aments and capsules of treated poplars and willows. It was found that the effect of the injection lasts two years. The results of the main experiment of 1987 can be summarized as follows ; First, the injection of the mixture of 2-chloroethane phosphonic acid and GA increases the premature abscisin of aments and capsules, thus reducing the wind dispersal of the cottony seeds of S. pseudolasiogyne, P. tomentiglandulosa, and P. euramericana 1644.09, 1200.61, and 1485.11 times, respectively, than that of the naural abscisin, It is estimated that the average number of wind-blown seeds reduced are approximatively 6,185,100, 4062,900, and 2,830,670 per tree, respectively. Second, the treatment causes no observable side effects on the growth of the samples tested.

• Archives of Pharmacal Research
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• v.26 no.7
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• pp.526-531
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• 2003

KR-31378 is a new drug candidate intended for the use in the prevention of ischemia-reperfusion damage. The objective of this preformulation study was to determine the physicochemical properties of KR-31378. The n-octanol to water partition coefficients of KR-31378 were 0.0504 at pH 3 and 0.8874 at pH 10. Accelerated stability of KR-31378 in solution and solid state was studied at 5, 40, $60^{\circ}C$. The stability testing indicated that the t90 for the drug in solid was estimated to be 2 years and 128.6 days at $25^{\circ}C$, while the that in aquesou solution was 68.6 days at $25^{\circ}C$. The KR-31378 was also found to be unstable under the relative humidity of 76%, probably because of the hygroscopic nature of the drug. In order to study compatibility of KR-31378 with typical excipients, potential change in differential scanning calorimetry spectrum was studied in 1:1 binary mixtures of KR-31378 and Aerosil, Avicel, Eudragit, lactose, PEG, talc, CMC, PVP, starch. As a result, CMC, PVP, and starch were found to be incompatible with KR-31378, indicating the addition of these excipients may complicate the manufacturing of the formulation for the drug. Particle size distribution of KR-31378 powder was in the size range of 9-93 $\mu$ m with the mean particle size of 37.9 $\mu$ m. The flowability of KR-31378 was apparently inadequate, indicating the granulation may be necessary for the processing of the drug to solid dosage forms. Crystallization of the drug with a number of organic solvents did not lead a crystalline polymorphism. In addition, dissolution of the drug from the powder was adequately rapid at $37^{\circ}C$ in water.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.53-61
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• 1999

This study was conducted to determine the optimal collecting time and methods of utilization of forest topsoil as a seed-bank source for revegetation materials of slopes. From April to November of 1998, nursery seedbeds in the greenhouse of Seoul National University were seeded with mixtures of forest topsoil and seeds. The nursery seedbeds were treated to evaluate the effect of depths of forest topsoil and seeding amounts. Seasonal experiments with $3{\times}3$ factorial design were conducted in April, June and August. More than 40 seedlings/$m^2$ were observed as the naturally-emerged species in the plot, treated with the top-layer forest soil within the depth of 10cm. However, it was found, as seeding more seeds, the number of appearance of the naturally-emerged species reduced because of the heavy competition between the seeded and the naturally-emerged. According to the results of this research, it is recommended that seeding amount should be no more than 1,000 seeds/$m^2$ to avoid the severe competition. Also it was observed that the forest topsoil collected in spring(April) is better than that collected in summer. The more species of the naturally-emerged were found in the forest topsoil collected in spring. Forest-topsoil-mixed-materials as a seed-bank source could significantly increase the plant diversity and productivity. Effective use of forest topsoil as a seed-bank source may become a valuable tool in future restoration of disturbed slopes for promoting plant community diversity and recycling of spoiled-soils from the slopes.

• Structural Engineering and Mechanics
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• v.85 no.1
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• pp.1-14
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• 2023

The use of functionally graded materials in applications involving severe thermal gradients is quickly gaining acceptance in the composite mechanics community, the aerospace and aircraft industry. In the present study, a refined sandwich plate model is applied to study the free vibration analysis of porous functionally graded material (FGM) sandwich plates with various distribution rate of porosity. Two types of common FG sandwich plates are considered. The first sandwich plate is composed of two FG material (FGM) face sheets and a homogeneous ceramic or metal core. The second one consists of two homogeneous fully metal and ceramic face sheets at the top and bottom, respectively, and a FGM core. The displacement field of the present theory is chosen based on nonlinear variations in the in-plane displacements through the thickness of the sandwich plate. The number of unknowns and equations of motion of the present theory is reduced and hence makes them simple to use. In the analysis, the equation of motion for simply supported sandwich plates is obtained using Hamilton's principle. In order to present the effect of the variation of the porosity distribution on the dynamic behavior of the FGM sandwich plates, new mixtures are proposed which take into account different rate of porosity distribution between the ceramic and the metal. The present method is applicable to study the dynamic behavior of FGM plates and sandwich plates. The frequencies of two kinds of FGM sandwich structures are analyzed and discussed. Several numerical results have been compared with the ones available in the literature.

• Bulletin of the Korean Chemical Society
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• v.28 no.1
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• pp.108-114
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• 2007

The lyotropic behaviors to form the structure of distearoylphosphatidylcholine (DSPC)-based liquid crystal (LC) hydrated by only propylene glycol (PG) without water were examined by differential scanning calorimetry (DSC), X-ray diffractions (XRD), polarized microscope (PM) and transmission electron microscope (TEM). By increasing the amount of PG instead of water, it showed the phase transition to be gradually changed from anisotropic structures to other structures more close to isotropic ones and their appearance to be changed from solid-like states to liquid-like ones with more fluidity. Below 50% w/w PG, the mixtures of DSPC and PG resulted in no direct observation of LC structure through PM because they were very close to solid-states. From 55% w/w to 90% w/w of PG, the dense lamella crystalline structures were observed through PM, and their thickness and area decreased as the content of PG increased. Measured by DSC with heating process, the main phase transition from α -lamella phase to isotropic phase appeared from 52.89 °C to 47.41 °C to show linearly decreasing behaviors because PG affects the hydrophobic region of DSPC-based lamella phase. The repeating distance of the lamella phase and the interlayer distance between bilayers were calculated with XRDs and the average number of bilayers related to the thickness in LC structure was approximately estimated by combining with TEM results. The WAXS and DSC measurements showed that all of PG molecules contributed to swelling both the lipid layer in the edge region of lamella phase close to phosphate groups and the interlayer between bilayers below 90% w/w of PG. The phase and thermal behaviors were found to depend on the amount of PG used by means of dissolving DSPC as a phospholipid and rearranging its structure. Instead of water, the inducement of PG as a polar solvent in solid-lamella phase is discussed in terms of the swelling effect of PG for DSPC-based lamella membrane.

• Mass Spectrometry Letters
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• v.5 no.3
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• pp.63-69
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• 2014

In this study, we present a new isotope-coded carbamidomethylation (iCCM)-based quantitative proteomics, as a complementary strategy for conventional isotope labeling strategies, with providing the simplicity, ease of use, and robustness. In iCCM-based quantification, two proteome samples can be separately isotope-labeled by means of covalently reaction of all cysteinyl residues in proteins with iodoacetamide (IAA) and its isotope (IAA-$^{13}C_2$, $D_2$), denoted as CM and iCCM, respectively, leading to a mass shift of all cysteinyl residues to be + 4 Da. To evaluate iCCM-based isotope labeling in proteomic quantification, 6 protein standards (i.e., bovine serum albumin, serotransferrin, lysozyme, beta-lactoglobulin, beta-galactosidase, and alpha-lactalbumin) isotopically labeled with IAA and its isotope, mixed equally, and followed by proteolytic digestion. The resulting CM-/iCCM-labeled peptide mixtures were analyzed using a nLC-ESI-FT orbitrap-MS/MS. From our experimental results, we found that the efficiency of iCCM-based quantification is more superior to that of mTRAQ, as a conventional nonisobaric labeling method, in which both of a number of identified peptides from 6 protein standards and the less quantitative variations in the relative abundance ratios of heavy-/light-labeled corresponding peptide pairs. Finally, we applied the developed iCCM-based quantitative method to lung cancer serum proteome in order to evaluate the potential in biomarker discovery study.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.151-161
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• 2019

Ashwagandha (Withania sominifera Duanal) is an important medicinal herb with increased demand after discovering its anti-stress and sex stimulating properties that are attributed to the presence of biologically active alkaloid compounds. The aim of this study was to elucidate a proper agro technological package that ensures the optimum growth of Ashwagandha to obtain the finest quality without degrading the pharmacologically active constituents. Mixtures of organic and inorganic fertilizers were combined with direct seeding and transplanted as four different treatments in this study. The fresh and dry weights of the tubers were recorded up to 12 months starting from two months after sowing (MAS) while the shoot height, root length, number of leaves, fresh and dry weights of the shoot and the root with a shoot ratio of up to 6 MAS were determined. The results revealed that the growth of Ashwagandha was not affected significantly by the method of planting, type of fertilizer or their combinations during most of the harvests. However, tubers harvested at 6 MAS had the highest recorded dry tuber weight per plant in all four treatments compared to the early harvests where two direct seeded treatments had the best results. Comparison of the phytochemical compounds showed that direct seeding with organic fertilizer had the highest recorded values for alkaloid and withaferine A contents with a lower percentage of fiber compared to the treatments with inorganic fertilizer. In conclusion, direct seeding with organic fertilizer and tubers harvested at 6 MAS are recommended as the best cultivation conditions and harvesting stage to obtain high quality tubers of Ashwagandha, respectively.

• IMMUNE NETWORK
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• v.22 no.1
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• pp.3.1-3.21
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• 2022

Cancer is one of the leading causes of death worldwide and the number of cancer patients is expected to continuously increase in the future. Traditional cancer therapies focus on inhibiting cancer growth while largely ignoring the contribution of the immune system in eliminating cancer cells. Recently, better understanding of immunological mechanisms pertaining to cancer progress has led to development of several immunotherapies, which revolutionized cancer treatment. Nonetheless, only a small proportion of cancer patients respond to immunotherapy and maintain a durable response. Among multiple factors contributing to the variability of immunotherapy response rates, commensal microbiota inhabiting patients have been identified as one of the most critical factors determining the success of immunotherapy. The functional diversity of microbiota differentially affects the host immune system and controls the efficacy of immunotherapy in individual cancer patients. Moreover, clinical studies have demonstrated that changing the gut microbiota composition by fecal microbiota transplantation in patients who failed a previous immunotherapy converts them to responders of the same therapy. Consequently, both academic and industrial researchers are putting extensive efforts to identify and develop specific bacteria or bacteria mixtures for cancer immunotherapy. In this review, we will summarize the immunological roles of commensal microbiota in cancer treatment and give specific examples of bacteria that show anticancer effect when administered as a monotherapy or as an adjuvant agent for immunotherapy. We will also list ongoing clinical trials testing the anticancer effect of commensal bacteria.