• International Journal of Reliability and Applications
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• 제1권1호
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• pp.65-79
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• 2000

This research considers a system which has an ultimate terminal event such as death, critical failure, bankruptcy together with a certain indicative events (temporary malfunction, special treatment, kind of defaults) that frequently occurs before the terminal event comes to the system. Some investigation of a model for the corresponding bivariate data of the system have been done with an explanation of the situation in terms of two continuous variables instead of continuous-discrete variables and some other properties. Also an analysis has been carried out to evaluate the effect of intermediate observation of occurrence of indicative event so that the result can be used for a possible suggestion of an intermediate observing schedule.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.384-391
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• 2009

Previously, two events (H15 and B20) of transgenic pepper (Capsicum annuum L.) that enhanced resistance to Cucumber mosaic virus (CMV) by the introduction of CMV coat protein (CP) gene were constructed. Presently, a single copy number of the CP gene was revealed in H15 and B20 by Southern blot. To predict possible unintended effects due to transgene insertion in an endogenous gene, we carried out sequencing of the 5'-flanking region of the CP gene and a Blastbased search. The results revealed that insertion of the transgene into genes encoding putative proteins may occur in the H15 and B20 transgenic event. Mutiplex polymerase chain reaction (PCR) for simultaneous detection and identification of transgenic pepper was conducted with a set of nine primers. Both transgenic event were differentiated from non-transgenic event by the presence of 267 bp and 430 bp PCR products indicative of CP gene specific primer pairs and primer pairs targeting the CP gene and 35S promoter. H15 and B20 uniquely possessed a 390 bp and 596 bp PCR product, respectively. The presence of a 1115 bp product corresponding to intrinsic pepper actin gene confirmed the use of pepper DNA as the PCR template. The primer set and PCR conditions used presently may allow the accurate and simple identification of CMV resistant transgenic pepper.