• Title/Summary/Keyword: nuclear ribosomal DNA

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Re-description of Chrysaora pacifica (Goette, 1886) (Cnidaria, Scyphozoa) from Korean Coastal Waters: Morphology and Molecular Comparisons

  • Lee, Hye Eun;Yoon, Won Duk;Chae, Jinho;Ki, Jang-Seu
    • Ocean and Polar Research
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    • v.38 no.4
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    • pp.295-301
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    • 2016
  • The nomenclature of the sea nettle jellyfish from Korea was initially described as Dactylometra quinquecirrha Agassiz, 1862. However its identity has been questioned on the basis of its local distribution and molecular data. Here, we examined morphology and DNA sequences of nuclear rDNA using specimens collected from southern Korean waters in August 2014. Based on morphological characteristics (bell size, umbrella pattern, number of tentacles and lappets) and distribution locality, we reassign the Korean D. quinquecirrha to Chrysaora pacifica (Goette, 1886), and provide a re-description accordingly. The molecular identity of C. pacifica was further confirmed by comparison of nuclear ribosomal DNA sequences.

PCR-mediated RFLP to Identify 'Bangpoong', a Crude Drug (유전자 감식에 의한 방풍(防風)의 감별)

  • Choi, Ho-Young;Lee, Sang-In;Suh, Young-Bae
    • Korean Journal of Pharmacognosy
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    • v.28 no.1
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    • pp.1-8
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    • 1997
  • Bangpoong (防風) is a popular crude drug used to expel wind from the body surface (祛風解表) to remove dampness (勝濕). And to relieve pain (正痛) and spasm (正痙). In China and Japan, roots of Saposhnikovia divaricata (Turcz.) Schischk. Is used as Bangpoong. However, the roots of Peucedanum japonicum Thunb. Or Glehnia littoralis (A. Gray) Fr. Schmidt ex Miquel, being called Sikbangpoong (植防風) and Wonbangpoong (元防風) respectively are used instead of Bangpoong in Korea. The ITS regions of nuclear ribosomal DNA were analyzed to determine original plants and to design a molecular identification method for the crude drugs used as Bangpoong in Korea and China. It is demonstrated that RFLP analysis via PCR has the great potential as a novel tool to test crude drugs for the quality control and standardization.

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Comparative Analysis of ITS Sequences from Acer Species (Aceraceae) in Korea

  • Suh, Young-Bae;Cho, Hye-Jeong;Kim, Sang-Tae;Park, Chong-Wook
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.1-8
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    • 1996
  • Sequences from the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA were determined to assess their potential as a phylogenetic tool for Korean Acer species, including A. okamotoannum and A. takesimense which are endemic on Ullung Island of volcanic origin. Although the genus Acer has been studied by various authors, different infrageneric dispositions have been suggested, and the phylogeny of the genus has been in dispute. The variation of ITS sequences from seven species of Acer was very low among species within the same section, but comparative analysesof the molecular data obtained suggest that ITS sequences may provide enough phylogenetic resolution for sectional relationships in the genus.

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Molecular Characterization of Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae) from Goats in the Western Part of India by LSU of Nuclear Ribosomal DNA

  • Kumar, Ashwani;Chaudhary, Anshu;Verma, Chandni;Singh, Hridaya Shanker
    • Parasites, Hosts and Diseases
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    • v.52 no.6
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    • pp.701-705
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    • 2014
  • The rumen parasite, Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae), is a highly pathogenic trematode parasite of goat (Capra hircus). It sucks blood that causes acute disease like anemia, and severe economic losses occur due to morbidity and mortality of the ruminant infected by these worms. The study of these rumen paramphistomes, their infection, and public health importance remains unclear in India especially in the western part of state Uttar Pradesh (U.P.), Meerut, India, where the goat meat consumption is very high. This paper provides the molecular characterization of G. crumenifer recovered from the rumen of Capra hircus from Meerut, U.P., India by the partial sequence of 28S rDNA. Nucleotide sequence similarity searching on BLAST of 28S rDNA from parasites showed the highest identity with those of G. crumenifer from the same host Capra hircus. This is the first report of molecular identification of G. crumenifer from this part of India.

A new species of Zabelia(Linnaeaceae) from Korea

  • Hong, Moon-Pyo;Kim, Young-Chul;Nam, Gi-Heum;Lee, Byoung Yoon
    • Journal of Species Research
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    • v.1 no.1
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    • pp.1-3
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    • 2012
  • A new species, Zabelia densipila M.-P. Hong, Y.-C. Kim & B.Y. Lee (Linnaeaceae) is described from Gangwon-do, Korea and illustrated. The new taxon resembles Z. biflora (Turcz.) Makino, but it can be distinguished by its different flowering seasons, ovaries with densely long-pilose hairs, and sequence differences in nuclear ribosomal DNA and chloroplast DNA coding regions.

Population Genetic Structure of the Bumblebee, Bombus ignitus (Hymenoptera: Apidae), Based on Mitochondrial COI Gene and Nuclear Ribosomal ITS2 Sequences

  • Oh, Hyung Keun;Yoon, Hyung Joo;Lee, Joo Young;Park, Jeong Sun;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.27 no.1
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    • pp.142-158
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    • 2013
  • The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.